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The map label for this gene is mdlA [H]

Identifier: 29144101

GI number: 29144101

Start: 3941984

End: 3943180

Strand: Reverse

Name: mdlA [H]

Synonym: t3822

Alternate gene names: 29144101

Gene position: 3943180-3941984 (Counterclockwise)

Preceding gene: 29144103

Following gene: 161486772

Centisome position: 82.29

GC content: 54.47

Gene sequence:

>1197_bases
ATGGCTTTAAGCGCGAATTCCGACGCCGTAACGTATGCAAAAGCGGCGAATACCAGAACCGCGGCAGAAACCGGCGATCG
TATCGAATGGGTGAAGCTATCACTGGCTTTTCTACCGCTGGCGACGCCAGTGAGTGACGCGAAGGTACTGACCGGTCGCC
AGAAACCTTTGACCGAAGTGGCAATCATCATTGCCGAAATCCGCAGTCGCGATGGCTTTGAGGGCGTTGGTTTTAGCTAC
TCCAAACGTGCTGGCGGCCAGAGTATTTATGCTCACGCCAAAGAGATAGCCGACAATCTACTGGGCGAAGACCCCAATGA
TATCGACAAAATATACACCAAGCTGCTGTGGGCCGGAGCCTCGGTGGGGCGTAGCGGGATGGCGGTACAGGCTATCTCCC
CTATCGATATCGCCTTATGGGATATGAAGGCTAAACGTGCCGGTCTGCCACTGGCAAAACTGTTGGGCGCGCACCGCGAC
TCCGTTCAGTGTTACAACACCTCAGGGGGGTTCTTGCATACACCGCTCGATCAAGTGCTGAAAAATGTGGTGATTTCCCG
CGAAAATGGCATTGGCGGTATTAAGTTGAAAGTCGGACAACCCAACTGCGCTGAGGATATTCGCCGCTTAACCGCAGTAC
GTGAAGCGCTTGGGGATGAGTTCCCGTTAATGGTTGACGCTAACCAGCAGTGGGATCGCGAAACGGCTATCCGCATGGGG
CGTAAAATGGAACAGTTCAATCTTATCTGGATTGAAGAACCATTAGATGCTTACGACATCGAAGGCCACGCCCAGCTTGC
TGCCGCGCTGGATACGCCTATCGCCACCGGGGAAATGCTGACCAGTTTCCGGGAACACGAGCAGTTGATTCTGGGCAATG
CCAGCGATTTCGTTCAGCCAGATGCGCCGCGTGTCGGCGGTATCTCTCCGTTCCTGAAGATTATGGATCTGGCGGCGAAA
CACGGGCGTAAGCTGGCGCCGCACTTTGCGATGGAAGTACACCTGCACCTTTCCGCAGCGTATCCCCTGGAGCCGTGGCT
GGAACATTTCGAGTGGCTGAACCCATTATTCAACGAGCAACTTGAGCTGCGCGATGGCCGCATGTGGATTTCCGATCGTC
ATGGTCTGGGTTTCACGCTCAGTGAACAAGCGCGCCGCTGGACACAATTAACATGTGAATTTGGCAAACGCCCTTAA

Upstream 100 bases:

>100_bases
GTGATCAAATGTTCAAATCAACATCAACTTTCTGGATCCTGCGTTCGCTTAATAATAAAAATGTTAGCGCAATCATTTTG
TGTTTATCAGGAGAAAAAAA

Downstream 100 bases:

>100_bases
CCCGACTGGCGGGAATCCGTCCCGCCTCTCTTACCTTACAGGATGCATTCTGCCCTTATCTGAGGAGTAGATAATGAACA
CAATAATAAAGTGCACAAAA

Product: racemase

Products: NA

Alternate protein names: MR [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MALSANSDAVTYAKAANTRTAAETGDRIEWVKLSLAFLPLATPVSDAKVLTGRQKPLTEVAIIIAEIRSRDGFEGVGFSY
SKRAGGQSIYAHAKEIADNLLGEDPNDIDKIYTKLLWAGASVGRSGMAVQAISPIDIALWDMKAKRAGLPLAKLLGAHRD
SVQCYNTSGGFLHTPLDQVLKNVVISRENGIGGIKLKVGQPNCAEDIRRLTAVREALGDEFPLMVDANQQWDRETAIRMG
RKMEQFNLIWIEEPLDAYDIEGHAQLAAALDTPIATGEMLTSFREHEQLILGNASDFVQPDAPRVGGISPFLKIMDLAAK
HGRKLAPHFAMEVHLHLSAAYPLEPWLEHFEWLNPLFNEQLELRDGRMWISDRHGLGFTLSEQARRWTQLTCEFGKRP

Sequences:

>Translated_398_residues
MALSANSDAVTYAKAANTRTAAETGDRIEWVKLSLAFLPLATPVSDAKVLTGRQKPLTEVAIIIAEIRSRDGFEGVGFSY
SKRAGGQSIYAHAKEIADNLLGEDPNDIDKIYTKLLWAGASVGRSGMAVQAISPIDIALWDMKAKRAGLPLAKLLGAHRD
SVQCYNTSGGFLHTPLDQVLKNVVISRENGIGGIKLKVGQPNCAEDIRRLTAVREALGDEFPLMVDANQQWDRETAIRMG
RKMEQFNLIWIEEPLDAYDIEGHAQLAAALDTPIATGEMLTSFREHEQLILGNASDFVQPDAPRVGGISPFLKIMDLAAK
HGRKLAPHFAMEVHLHLSAAYPLEPWLEHFEWLNPLFNEQLELRDGRMWISDRHGLGFTLSEQARRWTQLTCEFGKRP
>Mature_397_residues
ALSANSDAVTYAKAANTRTAAETGDRIEWVKLSLAFLPLATPVSDAKVLTGRQKPLTEVAIIIAEIRSRDGFEGVGFSYS
KRAGGQSIYAHAKEIADNLLGEDPNDIDKIYTKLLWAGASVGRSGMAVQAISPIDIALWDMKAKRAGLPLAKLLGAHRDS
VQCYNTSGGFLHTPLDQVLKNVVISRENGIGGIKLKVGQPNCAEDIRRLTAVREALGDEFPLMVDANQQWDRETAIRMGR
KMEQFNLIWIEEPLDAYDIEGHAQLAAALDTPIATGEMLTSFREHEQLILGNASDFVQPDAPRVGGISPFLKIMDLAAKH
GRKLAPHFAMEVHLHLSAAYPLEPWLEHFEWLNPLFNEQLELRDGRMWISDRHGLGFTLSEQARRWTQLTCEFGKRP

Specific function: Probably Involved In The Degradation Of Homoserine Lactone (Hsl) Or Of A Metabolite Of Hsl That Signals Starvation. [C]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=328, Percent_Identity=27.1341463414634, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI42544117, Length=325, Percent_Identity=25.5384615384615, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI186972148, Length=167, Percent_Identity=34.1317365269461, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI1787864, Length=368, Percent_Identity=24.7282608695652, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI48994953, Length=269, Percent_Identity=28.2527881040892, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI226510960, Length=324, Percent_Identity=26.2345679012346, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.1.2.2 [H]

Molecular weight: Translated: 44058; Mature: 43927

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSANSDAVTYAKAANTRTAAETGDRIEWVKLSLAFLPLATPVSDAKVLTGRQKPLTEV
CCCCCCCCCEEEECCCCCCCHHHCCCCEEEEEEEEHHHCCCCCCCCCHHHCCCCCCHHHH
AIIIAEIRSRDGFEGVGFSYSKRAGGQSIYAHAKEIADNLLGEDPNDIDKIYTKLLWAGA
HHHHHHHHCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
SVGRSGMAVQAISPIDIALWDMKAKRAGLPLAKLLGAHRDSVQCYNTSGGFLHTPLDQVL
CCCCCCCEEEECCCCEEEEECCHHHHCCCCHHHHHCCCCCCCEEECCCCCEEECCHHHHH
KNVVISRENGIGGIKLKVGQPNCAEDIRRLTAVREALGDEFPLMVDANQQWDRETAIRMG
HHHHEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHC
RKMEQFNLIWIEEPLDAYDIEGHAQLAAALDTPIATGEMLTSFREHEQLILGNASDFVQP
CCHHHEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCHHCCCC
DAPRVGGISPFLKIMDLAAKHGRKLAPHFAMEVHLHLSAAYPLEPWLEHFEWLNPLFNEQ
CCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCC
LELRDGRMWISDRHGLGFTLSEQARRWTQLTCEFGKRP
EEECCCEEEEECCCCCCEEHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALSANSDAVTYAKAANTRTAAETGDRIEWVKLSLAFLPLATPVSDAKVLTGRQKPLTEV
CCCCCCCCEEEECCCCCCCHHHCCCCEEEEEEEEHHHCCCCCCCCCHHHCCCCCCHHHH
AIIIAEIRSRDGFEGVGFSYSKRAGGQSIYAHAKEIADNLLGEDPNDIDKIYTKLLWAGA
HHHHHHHHCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
SVGRSGMAVQAISPIDIALWDMKAKRAGLPLAKLLGAHRDSVQCYNTSGGFLHTPLDQVL
CCCCCCCEEEECCCCEEEEECCHHHHCCCCHHHHHCCCCCCCEEECCCCCEEECCHHHHH
KNVVISRENGIGGIKLKVGQPNCAEDIRRLTAVREALGDEFPLMVDANQQWDRETAIRMG
HHHHEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHC
RKMEQFNLIWIEEPLDAYDIEGHAQLAAALDTPIATGEMLTSFREHEQLILGNASDFVQP
CCHHHEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCHHCCCC
DAPRVGGISPFLKIMDLAAKHGRKLAPHFAMEVHLHLSAAYPLEPWLEHFEWLNPLFNEQ
CCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCC
LELRDGRMWISDRHGLGFTLSEQARRWTQLTCEFGKRP
EEECCCEEEEECCCCCCEEHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2831968; 2271624; 2215699; 1909893; 1892834; 7893689; 7893690 [H]