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The map label for this gene is yibK [H]

Identifier: 29144103

GI number: 29144103

Start: 3944571

End: 3945044

Strand: Reverse

Name: yibK [H]

Synonym: t3824

Alternate gene names: 29144103

Gene position: 3945044-3944571 (Counterclockwise)

Preceding gene: 29144104

Following gene: 29144101

Centisome position: 82.33

GC content: 56.33

Gene sequence:

>474_bases
ATGCTTAACATCGTACTCTTCGAACCAGAAATCCCGCCGAATACCGGCAACATCATTCGTCTTTGCGCCAATACCGGCTT
TCGTCTGCACATCATCGAACCGATGGGGTTTGCCTGGGATGATAAGCGCTTACGCCGCGCAGGGTTGGACTATCACGAAT
TCGCCGCGGTTCAGCGCCATCACGATTATGCCGCCTTTGTCGCCGCCGAAAATCCACAGCGCCTGTTTGCGTTGACGACA
AAGGGCACGCCCGCCCATAGCGCCGTAAGCTATCAGGATGGCGATTATTTGATGTTTGGCCCGGAAACGCGCGGCCTGCC
CGCCAGCATTCTCGATGCGTTGCCTAAAGAGCAGAAGATTCGCATTCCGATGATGCCGGATAGCCGTAGCATGAACCTGT
CGAATGCGGTGTCGGTGGTGGTGTATGAAGCGTGGCGCCAGTTAGGATACCCCGGCGCGGTATTGCGGTCGTAA

Upstream 100 bases:

>100_bases
GCGCTGGCGCCAATGAGCAAAGGCGATGCCGCCTGAACGTCTGGCGCGTGAGAGCCCGGTAAATTTCTGCTATTCTGCCC
CCCGCATTTAAGGGGGCATC

Downstream 100 bases:

>100_bases
TATATTCAATGCCGGATGGCGGCTAACGCCTGATCCGGCCTGGCCGATCCCGATGGCATGTTCCCGTTTATGCGGACTCT
CGCTCAATCAAATGAAAGCC

Product: tRNA/rRNA methyltransferase YibK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFAAVQRHHDYAAFVAAENPQRLFALTT
KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPKEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRS

Sequences:

>Translated_157_residues
MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFAAVQRHHDYAAFVAAENPQRLFALTT
KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPKEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRS
>Mature_157_residues
MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFAAVQRHHDYAAFVAAENPQRLFALTT
KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPKEQKIRIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRS

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=156, Percent_Identity=94.8717948717949, Blast_Score=311, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17611; Mature: 17611

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFAAVQRH
CEEEEEECCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHC
HDYAAFVAAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPKEQKI
CCEEEEEEECCCCEEEEEEECCCCCCCEEEECCCCEEEECCCCCCCCHHHHHCCCCCCEE
RIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRS
EECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure
MLNIVLFEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFAAVQRH
CEEEEEECCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHC
HDYAAFVAAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPKEQKI
CCEEEEEEECCCCEEEEEEECCCCCCCEEEECCCCEEEECCCCCCCCHHHHHCCCCCCEE
RIPMMPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRS
EECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]