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The map label for this gene is smpB [H]

Identifier: 29143011

GI number: 29143011

Start: 2717618

End: 2718100

Strand: Direct

Name: smpB [H]

Synonym: t2642

Alternate gene names: 29143011

Gene position: 2717618-2718100 (Clockwise)

Preceding gene: 29143008

Following gene: 29143012

Centisome position: 56.71

GC content: 49.69

Gene sequence:

>483_bases
ATGACGAAGAAAAAAGCACATAAACCGGGCTCAGCCACCATCGCGCTGAATAAGCGCGCCCGGCACGAATACTTTATCGA
AGAAGAGTTCGAGGCTGGTCTCGCCCTGCAAGGCTGGGAAGTAAAATCTCTGCGCGCAGGAAAAGCCAATATCGGCGACA
GTTACGTTATCCTCAAAGATGGCGAGGCCTGGCTGTTTGGCGCAAACTTTACGCCGATGGCGGTCGCCTCGACACATGTC
GTATGCGACCCAACCCGAACCCGCAAACTGTTGTTAAATCAGCGTGAGCTTGATTCGCTGTATGGCCGTATTAATCGTGA
AGGTTATACTGTCGTCGCGCTTTCTCTGTACTGGAAAAATGCCTGGTGCAAAGTGAAGATCGGCGTGGCGAAAGGTAAAA
AACAACATGATAAACGTTCCGATCTTAAAGAACGTGAGTGGCAACTGGATAAAGCACGCATTATGAAAAATGCAGGCCGA
TAA

Upstream 100 bases:

>100_bases
TGCGTGCATAGCCCAACTCATGCTAAGATAGATTTTTGCACCTCATCAGGACGTAATGGGGTGTTTTCGATATCAGATTA
CCTATGAATTCACGACACTT

Downstream 100 bases:

>100_bases
TAGACATTTTTTGTGACCAGCTTCTCAGTATGAAGCTGGTCAATGTTCATATCTCATGCATGCCTCGAAAATATATTCTC
TGTGTCTGACGCTTATCAAT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSLRAGKANIGDSYVILKDGEAWLFGANFTPMAVASTHV
VCDPTRTRKLLLNQRELDSLYGRINREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDLKEREWQLDKARIMKNAGR

Sequences:

>Translated_160_residues
MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSLRAGKANIGDSYVILKDGEAWLFGANFTPMAVASTHV
VCDPTRTRKLLLNQRELDSLYGRINREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDLKEREWQLDKARIMKNAGR
>Mature_159_residues
TKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSLRAGKANIGDSYVILKDGEAWLFGANFTPMAVASTHVV
CDPTRTRKLLLNQRELDSLYGRINREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDLKEREWQLDKARIMKNAGR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=160, Percent_Identity=94.375, Blast_Score=317, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18232; Mature: 18101

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSLRAGKANIGDSYVILKD
CCCCCCCCCCCEEEEECCCCCCCCCCCHHHCCCEEECCCEEEEEECCCCCCCCEEEEEEC
GEAWLFGANFTPMAVASTHVVCDPTRTRKLLLNQRELDSLYGRINREGYTVVALSLYWKN
CCEEEEECCCCCEEEECEEEEECCCHHHHHHHCHHHHHHHHHHCCCCCEEEEEEEEEECC
AWCKVKIGVAKGKKQHDKRSDLKEREWQLDKARIMKNAGR
CEEEEEEECCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSLRAGKANIGDSYVILKD
CCCCCCCCCCEEEEECCCCCCCCCCCHHHCCCEEECCCEEEEEECCCCCCCCEEEEEEC
GEAWLFGANFTPMAVASTHVVCDPTRTRKLLLNQRELDSLYGRINREGYTVVALSLYWKN
CCEEEEECCCCCEEEECEEEEECCCHHHHHHHCHHHHHHHHHHCCCCCEEEEEEEEEECC
AWCKVKIGVAKGKKQHDKRSDLKEREWQLDKARIMKNAGR
CEEEEEEECCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA