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The map label for this gene is gloB [H]

Identifier: 29142978

GI number: 29142978

Start: 2680139

End: 2680894

Strand: Direct

Name: gloB [H]

Synonym: t2604

Alternate gene names: 29142978

Gene position: 2680139-2680894 (Clockwise)

Preceding gene: 29142976

Following gene: 29142979

Centisome position: 55.93

GC content: 43.78

Gene sequence:

>756_bases
ATGAATCTTAACAGTATTCCCGCGTTTCAGGACAATTACATCTGGGTGCTGACCAATGATGAAGGCCGCTGTGTGATTGT
TGATCCCGGCGAAGCGGCCCCCGTCTTAAAGGCCATTGCTGCACATAAGTGGATGCCGGAGGCGATTTTTCTGACGCATC
ACCATTACGACCATGTTGGCGGAGTCAAAGAGCTGTTGCAACACTTCCCGCAAATGACGGTTTATGGACCGGCGGAAACG
CAAGACAAGGGAGCAACCCATCTTGTTGGCGATGGCGATACTATTCGCGTTTTAGGCGAGAAATTTACTCTTTTTGCCAC
GCCGGGCCACACGTTAGGACACGTCTGTTACTTTAGCCATCCTTACTTGTTCTGCGGCGACACGCTGTTCTCTGGCGGCT
GTGGTAGATTGTTTGAAGGCACGCCATCACAGATGTATCAGTCACTTATGAAAATTAACTCTCTGCCTGACGACACACTC
ATTTGCAGCGCTCACGAATACACTTTAGCTAACATTAAGTTCGCATTGAGCATACTTCCGCACGATTCGTTCATAAATGA
ATATTATCGTAAAGTTAAAGAGTTACGTGTAAAAAAACAAATGACACTACCCGTTATTCTTAAAAATGAGCGTAAGATTA
ATCTTTTTTTAAGAACTGAAGATATTGATTTAATTAATGAAATAAACAAAGAAACAATATTGCAACAACCAGAAGCGCGT
TTTGCATGGTTAAGGTCAAAGAAAGACACGTTCTGA

Upstream 100 bases:

>100_bases
GCCCCAGGGCAACTCACCCCAACATCCGGGCGCAACGACAGTTTGAGAGAGCCTTGCCGGTTTCATCGCAACCTTCCGCT
AATTATTTGAGAGGTAATAT

Downstream 100 bases:

>100_bases
TAATTCTTACTTGTCATTCGCTAACTTCGCCGTTATGATCGGTCGTCTTTTAAGCAACTATTGACACACACATGAAGGCA
AAAGCGATATTACTCGCCTC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNLNSIPAFQDNYIWVLTNDEGRCVIVDPGEAAPVLKAIAAHKWMPEAIFLTHHHYDHVGGVKELLQHFPQMTVYGPAET
QDKGATHLVGDGDTIRVLGEKFTLFATPGHTLGHVCYFSHPYLFCGDTLFSGGCGRLFEGTPSQMYQSLMKINSLPDDTL
ICSAHEYTLANIKFALSILPHDSFINEYYRKVKELRVKKQMTLPVILKNERKINLFLRTEDIDLINEINKETILQQPEAR
FAWLRSKKDTF

Sequences:

>Translated_251_residues
MNLNSIPAFQDNYIWVLTNDEGRCVIVDPGEAAPVLKAIAAHKWMPEAIFLTHHHYDHVGGVKELLQHFPQMTVYGPAET
QDKGATHLVGDGDTIRVLGEKFTLFATPGHTLGHVCYFSHPYLFCGDTLFSGGCGRLFEGTPSQMYQSLMKINSLPDDTL
ICSAHEYTLANIKFALSILPHDSFINEYYRKVKELRVKKQMTLPVILKNERKINLFLRTEDIDLINEINKETILQQPEAR
FAWLRSKKDTF
>Mature_251_residues
MNLNSIPAFQDNYIWVLTNDEGRCVIVDPGEAAPVLKAIAAHKWMPEAIFLTHHHYDHVGGVKELLQHFPQMTVYGPAET
QDKGATHLVGDGDTIRVLGEKFTLFATPGHTLGHVCYFSHPYLFCGDTLFSGGCGRLFEGTPSQMYQSLMKINSLPDDTL
ICSAHEYTLANIKFALSILPHDSFINEYYRKVKELRVKKQMTLPVILKNERKINLFLRTEDIDLINEINKETILQQPEAR
FAWLRSKKDTF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=260, Percent_Identity=32.6923076923077, Blast_Score=144, Evalue=8e-35,
Organism=Homo sapiens, GI94538322, Length=260, Percent_Identity=32.6923076923077, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI116642887, Length=227, Percent_Identity=35.2422907488987, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI21703352, Length=227, Percent_Identity=35.2422907488987, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI14150041, Length=229, Percent_Identity=32.7510917030568, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI46361987, Length=194, Percent_Identity=33.5051546391753, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1786406, Length=251, Percent_Identity=78.0876494023904, Blast_Score=412, Evalue=1e-117,
Organism=Escherichia coli, GI1787158, Length=192, Percent_Identity=29.6875, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17536925, Length=214, Percent_Identity=33.6448598130841, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17538952, Length=158, Percent_Identity=31.0126582278481, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320478, Length=232, Percent_Identity=30.6034482758621, Blast_Score=92, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324614, Length=231, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24667711, Length=261, Percent_Identity=34.0996168582375, Blast_Score=157, Evalue=4e-39,
Organism=Drosophila melanogaster, GI21356335, Length=261, Percent_Identity=34.0996168582375, Blast_Score=157, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24667703, Length=261, Percent_Identity=34.0996168582375, Blast_Score=157, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28566; Mature: 28566

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNSIPAFQDNYIWVLTNDEGRCVIVDPGEAAPVLKAIAAHKWMPEAIFLTHHHYDHVG
CCCCCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHH
GVKELLQHFPQMTVYGPAETQDKGATHLVGDGDTIRVLGEKFTLFATPGHTLGHVCYFSH
HHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCEEEEEECEEEEEECCCCHHHEEEEECC
PYLFCGDTLFSGGCGRLFEGTPSQMYQSLMKINSLPDDTLICSAHEYTLANIKFALSILP
CEEEECCCHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEEEEEEECC
HDSFINEYYRKVKELRVKKQMTLPVILKNERKINLFLRTEDIDLINEINKETILQQPEAR
CHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEECCHHHHHHCCHHHHHCCCCHH
FAWLRSKKDTF
HHHHHCCCCCC
>Mature Secondary Structure
MNLNSIPAFQDNYIWVLTNDEGRCVIVDPGEAAPVLKAIAAHKWMPEAIFLTHHHYDHVG
CCCCCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHH
GVKELLQHFPQMTVYGPAETQDKGATHLVGDGDTIRVLGEKFTLFATPGHTLGHVCYFSH
HHHHHHHHCCCEEEECCCCCCCCCCEEEECCCCEEEEEECEEEEEECCCCHHHEEEEECC
PYLFCGDTLFSGGCGRLFEGTPSQMYQSLMKINSLPDDTLICSAHEYTLANIKFALSILP
CEEEECCCHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEEEEEEECC
HDSFINEYYRKVKELRVKKQMTLPVILKNERKINLFLRTEDIDLINEINKETILQQPEAR
CHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEECCHHHHHHCCHHHHHCCCCHH
FAWLRSKKDTF
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA