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The map label for this gene is rnhA [H]

Identifier: 29142976

GI number: 29142976

Start: 2678918

End: 2679385

Strand: Direct

Name: rnhA [H]

Synonym: t2602

Alternate gene names: 29142976

Gene position: 2678918-2679385 (Clockwise)

Preceding gene: 29142974

Following gene: 29142978

Centisome position: 55.9

GC content: 50.21

Gene sequence:

>468_bases
ATGCTTAAACAGGTAGAAATTTTCACCGATGGCTCTTGCCTGGGGAATCCAGGGCCTGGTGGTTATGGCGCTATCTTACG
CTATCGCGGTCATGAAAAAACGTTTAGTGAAGGTTACACGCTGACCACCAATAACCGTATGGAACTGATGGCGGCGATCG
TCGCGCTTGAAGCGTTAAAAGAACATTGCGAAGTGACATTGAGCACCGACAGCCAATATGTGCGGCAAGGAATTACCCAA
TGGATTCATAACTGGAAGAAACGCGGCTGGAAAACAGCGGAAAAGAAACCTGTAAAAAATGTCGATCTCTGGAAACGTCT
CGATGCTGCGTTAGGCCAGCATCAGATCAAATGGGTATGGGTCAAAGGCCATGCAGGCCATCCCGAAAACGAGCGTTGTG
ATGAACTGGCGCGCGCCGCGGCAATGAATCCCACGCAGGAAGATAGTGGCTACCAGGCAGAAGCTTAG

Upstream 100 bases:

>100_bases
TATCGAGGACAATCTGTCGTGTAATTGCAGTGCTCATATCGGTCATTTATGTCAGACTTGCGTTTTGTTATCGATTTCAA
TTACAGGAAGTCTACCAGAG

Downstream 100 bases:

>100_bases
CCATCCGGTTTGCGATATTGCCGGGTGGCGCCAACGGTCTGGCGGATAGGGGTTTTACTTTTATTATGTCGCAGCGGATT
AAGCGTAAGCGGAATGGTCC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MLKQVEIFTDGSCLGNPGPGGYGAILRYRGHEKTFSEGYTLTTNNRMELMAAIVALEALKEHCEVTLSTDSQYVRQGITQ
WIHNWKKRGWKTAEKKPVKNVDLWKRLDAALGQHQIKWVWVKGHAGHPENERCDELARAAAMNPTQEDSGYQAEA

Sequences:

>Translated_155_residues
MLKQVEIFTDGSCLGNPGPGGYGAILRYRGHEKTFSEGYTLTTNNRMELMAAIVALEALKEHCEVTLSTDSQYVRQGITQ
WIHNWKKRGWKTAEKKPVKNVDLWKRLDAALGQHQIKWVWVKGHAGHPENERCDELARAAAMNPTQEDSGYQAEA
>Mature_155_residues
MLKQVEIFTDGSCLGNPGPGGYGAILRYRGHEKTFSEGYTLTTNNRMELMAAIVALEALKEHCEVTLSTDSQYVRQGITQ
WIHNWKKRGWKTAEKKPVKNVDLWKRLDAALGQHQIKWVWVKGHAGHPENERCDELARAAAMNPTQEDSGYQAEA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=142, Percent_Identity=36.6197183098592, Blast_Score=77, Evalue=6e-15,
Organism=Escherichia coli, GI1786408, Length=155, Percent_Identity=92.9032258064516, Blast_Score=304, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI115532542, Length=140, Percent_Identity=36.4285714285714, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17137476, Length=141, Percent_Identity=38.2978723404255, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45552509, Length=141, Percent_Identity=38.2978723404255, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 17510; Mature: 17510

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKQVEIFTDGSCLGNPGPGGYGAILRYRGHEKTFSEGYTLTTNNRMELMAAIVALEALK
CCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHCCEEEECCCHHHHHHHHHHHHHHH
EHCEVTLSTDSQYVRQGITQWIHNWKKRGWKTAEKKPVKNVDLWKRLDAALGQHQIKWVW
HHCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEE
VKGHAGHPENERCDELARAAAMNPTQEDSGYQAEA
EECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLKQVEIFTDGSCLGNPGPGGYGAILRYRGHEKTFSEGYTLTTNNRMELMAAIVALEALK
CCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHCCEEEECCCHHHHHHHHHHHHHHH
EHCEVTLSTDSQYVRQGITQWIHNWKKRGWKTAEKKPVKNVDLWKRLDAALGQHQIKWVW
HHCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEE
VKGHAGHPENERCDELARAAAMNPTQEDSGYQAEA
EECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA