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The map label for this gene is ddl

Identifier: 29142870

GI number: 29142870

Start: 2554963

End: 2556057

Strand: Direct

Name: ddl

Synonym: t2484

Alternate gene names: 29142870

Gene position: 2554963-2556057 (Clockwise)

Preceding gene: 29142864

Following gene: 29142872

Centisome position: 53.32

GC content: 52.24

Gene sequence:

>1095_bases
ATGGCGAAATTGCGGGTAGGAATAGTATTTGGCGGTAAGTCGGCGGAACATGAAGTGTCTTTGCAATCAGCGAAAAATAT
CGTTGATGCGATCGATAAAACCCGCTTCGACGTGGTGCTGTTAGGGATAGATAAGGCGGGACAATGGCATGTTAACGATG
CGGAAAATTATTTGCAGAATGCAGACGATCCGGCACATATCGCGCTACGTCCTTCGGCAATCAGTCTGGCCCAGGTGCCT
GGCAAACACCAACACCAATTGATTAACGCTCAGAACGGGCAGCCGCTACCGACGGTAGATGTTATTTTTCCTATCGTCCA
CGGTACGCTGGGCGAAGACGGCTCATTGCAGGGGATGCTGCGCGTCGCAAACTTACCGTTTGTCGGTTCAGACGTGCTGA
GCTCCGCAGCCTGCATGGATAAAGACGTCGCCAAACGACTGCTACGCGATGCAGGACTGAATATCGCCCCGTTTATTACA
CTCACCCGAACGAATCGTCACGCGTTTAGTTTTGCCGAGGTTGAATCCCGTCTGGGACTGCCGCTGTTCGTTAAGCCCGC
CAATCAGGGCTCTTCGGTCGGCGTCAGTAAAGTCGCTAACGAAGCGCAATATCAGCAGGCGGTCGCGCTGGCTTTCGAAT
TCGATCATAAAGTGGTGGTGGAGCAAGGGATCAAAGGGCGCGAGATCGAATGCGCCGTATTAGGTAACGATAACCCACAG
GCCAGTACCTGCGGCGAAATCGTACTGAACAGCGAATTTTACGCCTACGATACTAAGTATATTGATGATAATGGCGCGCA
AGTTGTCGTGCCTGCGCAGATCCCTTCCGAGGTGAATGACAAGATCCGCGCGATCGCGATCCAGGCTTATCAGACGCTGG
GGTGCGCGGGTATGGCGCGTGTCGATGTCTTCTTAACGGCAGACAATGAGGTCGTGATTAACGAGATTAATACGCTGCCA
GGTTTTACCAATATCAGTATGTATCCAAAACTCTGGCAGGCGAGCGGATTGGGCTATACCGATTTGATCAGCCGCCTGAT
TGAGCTGGCGCTGGAACGCCATACGGCGAATAACGCCTTAAAAACCACGATGTAA

Upstream 100 bases:

>100_bases
CCGTTCTCACATCCCATAAACATTATGGCAGAAGCCCGCGAAACCCGCGTTTTGTCGTGGTAATGTATACGCTTTGCATT
CGATGGAATGGGTTTTTAAG

Downstream 100 bases:

>100_bases
TAGCGCACGCCCGATAGCGCAACGTTTATCGGGCGTCTTTTACCTCATCATCTTCGCGCCGGCGAATGATGAACCCCGCC
AGCCAGAAGCTGATAATCCA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase A; D-alanylalanine synthetase A

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MAKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKTRFDVVLLGIDKAGQWHVNDAENYLQNADDPAHIALRPSAISLAQVP
GKHQHQLINAQNGQPLPTVDVIFPIVHGTLGEDGSLQGMLRVANLPFVGSDVLSSAACMDKDVAKRLLRDAGLNIAPFIT
LTRTNRHAFSFAEVESRLGLPLFVKPANQGSSVGVSKVANEAQYQQAVALAFEFDHKVVVEQGIKGREIECAVLGNDNPQ
ASTCGEIVLNSEFYAYDTKYIDDNGAQVVVPAQIPSEVNDKIRAIAIQAYQTLGCAGMARVDVFLTADNEVVINEINTLP
GFTNISMYPKLWQASGLGYTDLISRLIELALERHTANNALKTTM

Sequences:

>Translated_364_residues
MAKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKTRFDVVLLGIDKAGQWHVNDAENYLQNADDPAHIALRPSAISLAQVP
GKHQHQLINAQNGQPLPTVDVIFPIVHGTLGEDGSLQGMLRVANLPFVGSDVLSSAACMDKDVAKRLLRDAGLNIAPFIT
LTRTNRHAFSFAEVESRLGLPLFVKPANQGSSVGVSKVANEAQYQQAVALAFEFDHKVVVEQGIKGREIECAVLGNDNPQ
ASTCGEIVLNSEFYAYDTKYIDDNGAQVVVPAQIPSEVNDKIRAIAIQAYQTLGCAGMARVDVFLTADNEVVINEINTLP
GFTNISMYPKLWQASGLGYTDLISRLIELALERHTANNALKTTM
>Mature_363_residues
AKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKTRFDVVLLGIDKAGQWHVNDAENYLQNADDPAHIALRPSAISLAQVPG
KHQHQLINAQNGQPLPTVDVIFPIVHGTLGEDGSLQGMLRVANLPFVGSDVLSSAACMDKDVAKRLLRDAGLNIAPFITL
TRTNRHAFSFAEVESRLGLPLFVKPANQGSSVGVSKVANEAQYQQAVALAFEFDHKVVVEQGIKGREIECAVLGNDNPQA
STCGEIVLNSEFYAYDTKYIDDNGAQVVVPAQIPSEVNDKIRAIAIQAYQTLGCAGMARVDVFLTADNEVVINEINTLPG
FTNISMYPKLWQASGLGYTDLISRLIELALERHTANNALKTTM

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=364, Percent_Identity=89.8351648351648, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI1786280, Length=253, Percent_Identity=39.9209486166008, Blast_Score=184, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDLA_SALTI (P0A1F1)

Other databases:

- EMBL:   AL627266
- EMBL:   AE014613
- RefSeq:   NP_454975.1
- RefSeq:   NP_806212.1
- ProteinModelPortal:   P0A1F1
- SMR:   P0A1F1
- PRIDE:   P0A1F1
- GeneID:   1070527
- GeneID:   1246892
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t2484
- KEGG:   sty:STY0412
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- BioCyc:   SENT209261:T2484-MONOMER
- BioCyc:   SENT220341:STY0412-MONOMER
- BRENDA:   6.3.2.4
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 39357; Mature: 39226

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKTRFDVVLLGIDKAGQWHVNDAENYLQN
CCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHCCCEEEEEEEECCCCCEECCCHHHHHHC
ADDPAHIALRPSAISLAQVPGKHQHQLINAQNGQPLPTVDVIFPIVHGTLGEDGSLQGML
CCCCCEEEECCCCEEHHHCCCCHHHHEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEE
RVANLPFVGSDVLSSAACMDKDVAKRLLRDAGLNIAPFITLTRTNRHAFSFAEVESRLGL
EEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEECCCCEEEHHHHHHHCCC
PLFVKPANQGSSVGVSKVANEAQYQQAVALAFEFDHKVVVEQGIKGREIECAVLGNDNPQ
EEEEEECCCCCCCCHHHHHHHHHHHHHEEEEEECCCEEEECCCCCCCEEEEEEECCCCCC
ASTCGEIVLNSEFYAYDTKYIDDNGAQVVVPAQIPSEVNDKIRAIAIQAYQTLGCAGMAR
CCCCCCEEECCEEEEEECEEECCCCCEEEECCCCCCHHCCHHEEEEEEHHHHHCCCCEEE
VDVFLTADNEVVINEINTLPGFTNISMYPKLWQASGLGYTDLISRLIELALERHTANNAL
EEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCE
KTTM
ECCC
>Mature Secondary Structure 
AKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKTRFDVVLLGIDKAGQWHVNDAENYLQN
CEEEEEEEECCCCCCCEEHHHHHHHHHHHHHCCCEEEEEEEECCCCCEECCCHHHHHHC
ADDPAHIALRPSAISLAQVPGKHQHQLINAQNGQPLPTVDVIFPIVHGTLGEDGSLQGML
CCCCCEEEECCCCEEHHHCCCCHHHHEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEE
RVANLPFVGSDVLSSAACMDKDVAKRLLRDAGLNIAPFITLTRTNRHAFSFAEVESRLGL
EEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEECCCCEEEHHHHHHHCCC
PLFVKPANQGSSVGVSKVANEAQYQQAVALAFEFDHKVVVEQGIKGREIECAVLGNDNPQ
EEEEEECCCCCCCCHHHHHHHHHHHHHEEEEEECCCEEEECCCCCCCEEEEEEECCCCCC
ASTCGEIVLNSEFYAYDTKYIDDNGAQVVVPAQIPSEVNDKIRAIAIQAYQTLGCAGMAR
CCCCCCEEECCEEEEEECEEECCCCCEEEECCCCCCHHCCHHEEEEEEHHHHHCCCCEEE
VDVFLTADNEVVINEINTLPGFTNISMYPKLWQASGLGYTDLISRLIELALERHTANNAL
EEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCE
KTTM
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504