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The map label for this gene is ynfL [H]

Identifier: 29141861

GI number: 29141861

Start: 1452506

End: 1453405

Strand: Direct

Name: ynfL [H]

Synonym: t1408

Alternate gene names: 29141861

Gene position: 1452506-1453405 (Clockwise)

Preceding gene: 29141859

Following gene: 29141862

Centisome position: 30.31

GC content: 56.44

Gene sequence:

>900_bases
ATGAATATCGAATTACGTCATTTACGTTACTTTGTGGCGGTAGCAGAAGAGCTGCATTTTGGTCGCGCCGCCGCGCGCCT
CAATATCTCGCAGCCGCCGCTCAGTCAGCAGATACAAATTCTGGAGCAGCAGGTCGGTGCGCGATTACTGGCGCGGACCA
ACCGCAGCGTAGCGTTAACCGCCGCCGGGCGACAGTTTCTGGCGGATAGCCGACACATCCTGAGTCTGGTAAACGACGCC
GCCGCTCGCGCCGAGCGGTTGCATCAGGGCGAAGCGGGCGAAATCCGTATTGGTTTTACGTCTTCAGCGCCTTTTATTCG
GGCGGTATCGCATACCTTTTCGTTGTTCCGCCAGTCCTATCCGGGGGTGCATATGCAAACGCGTGAAATGAATACGCGTG
AGCAAATCGCGCCGCTCAACGAAGGCGTACTGGATATTGGCTTGCTGCGGAATACGCCGCTGCCAGACACACTTAATCGC
GAGGTGATTTTGCATGAGCCGCTTATGGCCATGATCCCGCGCGAACATCGGCTGGCGCAAAAGCCAGTCGTCAGTCTGAC
AGAACTGGCGGAAGAACCTTTCGTCTTTTTCGATCCGCATGTCGGGACGGGTCTCTACGACGATATTCTCGGCCTGATGC
GGCGCTATAACCTGTCGCCGACCATCACGCAGGAGGTTGGCGAGGCAATGACTATTATCGGTCTGGTGGCGGCGGGACTG
GGCGTCTCGATTCTGCCGGCATCCTTTAAGCGTGTACAGATGAGCGAGATGCGCTGGGTTCCCCTGGCAGAAGAGGACGC
CGTATCTGAAATGTGGCTGGTATGGCCAAAGCATCATGAACAGAGTCATGCCGTACAGCGGTTTTGCCAACTGCTATTAC
TGGCTGCCCGACAGGATTAA

Upstream 100 bases:

>100_bases
TCTCCGTCAACAGGAGACGGGTATAACCGACATGTAAAGCATAGAAAAAGCAAAATATTCTGTCTAATATATTAATTGTC
TCAAATAAGACGTTAAAAAT

Downstream 100 bases:

>100_bases
TTACGCTTAAAAAGGGTGAAAATGTGCAGTTAATCACACGGCTAAGCAAAAAGTTGACGGCGAGTATTGAAGTGTTTCAC
CATAGCACACAGATTATTTC

Product: regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQVGARLLARTNRSVALTAAGRQFLADSRHILSLVNDA
AARAERLHQGEAGEIRIGFTSSAPFIRAVSHTFSLFRQSYPGVHMQTREMNTREQIAPLNEGVLDIGLLRNTPLPDTLNR
EVILHEPLMAMIPREHRLAQKPVVSLTELAEEPFVFFDPHVGTGLYDDILGLMRRYNLSPTITQEVGEAMTIIGLVAAGL
GVSILPASFKRVQMSEMRWVPLAEEDAVSEMWLVWPKHHEQSHAVQRFCQLLLLAARQD

Sequences:

>Translated_299_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQVGARLLARTNRSVALTAAGRQFLADSRHILSLVNDA
AARAERLHQGEAGEIRIGFTSSAPFIRAVSHTFSLFRQSYPGVHMQTREMNTREQIAPLNEGVLDIGLLRNTPLPDTLNR
EVILHEPLMAMIPREHRLAQKPVVSLTELAEEPFVFFDPHVGTGLYDDILGLMRRYNLSPTITQEVGEAMTIIGLVAAGL
GVSILPASFKRVQMSEMRWVPLAEEDAVSEMWLVWPKHHEQSHAVQRFCQLLLLAARQD
>Mature_299_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQVGARLLARTNRSVALTAAGRQFLADSRHILSLVNDA
AARAERLHQGEAGEIRIGFTSSAPFIRAVSHTFSLFRQSYPGVHMQTREMNTREQIAPLNEGVLDIGLLRNTPLPDTLNR
EVILHEPLMAMIPREHRLAQKPVVSLTELAEEPFVFFDPHVGTGLYDDILGLMRRYNLSPTITQEVGEAMTIIGLVAAGL
GVSILPASFKRVQMSEMRWVPLAEEDAVSEMWLVWPKHHEQSHAVQRFCQLLLLAARQD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=297, Percent_Identity=83.5016835016835, Blast_Score=502, Evalue=1e-144,
Organism=Escherichia coli, GI2367136, Length=257, Percent_Identity=38.5214007782101, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1788887, Length=290, Percent_Identity=33.448275862069, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1790399, Length=248, Percent_Identity=30.6451612903226, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788748, Length=252, Percent_Identity=30.5555555555556, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI145693105, Length=294, Percent_Identity=26.1904761904762, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081904, Length=195, Percent_Identity=34.3589743589744, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI157672245, Length=297, Percent_Identity=28.6195286195286, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788297, Length=256, Percent_Identity=27.734375, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790208, Length=258, Percent_Identity=27.5193798449612, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789506, Length=295, Percent_Identity=23.0508474576271, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786713, Length=246, Percent_Identity=28.0487804878049, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787589, Length=129, Percent_Identity=37.2093023255814, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790262, Length=258, Percent_Identity=24.4186046511628, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787530, Length=269, Percent_Identity=24.1635687732342, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788481, Length=293, Percent_Identity=25.5972696245734, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787601, Length=254, Percent_Identity=28.3464566929134, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787806, Length=299, Percent_Identity=23.7458193979933, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33611; Mature: 33611

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQVGARLLARTNRSVALT
CCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
AAGRQFLADSRHILSLVNDAAARAERLHQGEAGEIRIGFTSSAPFIRAVSHTFSLFRQSY
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC
PGVHMQTREMNTREQIAPLNEGVLDIGLLRNTPLPDTLNREVILHEPLMAMIPREHRLAQ
CCCEEECCCCCCHHHHCCCCCCCEEEHHHCCCCCCCCCCCCEEEECCHHHHCCCHHHHHH
KPVVSLTELAEEPFVFFDPHVGTGLYDDILGLMRRYNLSPTITQEVGEAMTIIGLVAAGL
CHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
GVSILPASFKRVQMSEMRWVPLAEEDAVSEMWLVWPKHHEQSHAVQRFCQLLLLAARQD
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQVGARLLARTNRSVALT
CCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
AAGRQFLADSRHILSLVNDAAARAERLHQGEAGEIRIGFTSSAPFIRAVSHTFSLFRQSY
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC
PGVHMQTREMNTREQIAPLNEGVLDIGLLRNTPLPDTLNREVILHEPLMAMIPREHRLAQ
CCCEEECCCCCCHHHHCCCCCCCEEEHHHCCCCCCCCCCCCEEEECCHHHHCCCHHHHHH
KPVVSLTELAEEPFVFFDPHVGTGLYDDILGLMRRYNLSPTITQEVGEAMTIIGLVAAGL
CHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
GVSILPASFKRVQMSEMRWVPLAEEDAVSEMWLVWPKHHEQSHAVQRFCQLLLLAARQD
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]