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The map label for this gene is mlc [H]

Identifier: 29141862

GI number: 29141862

Start: 1453593

End: 1454753

Strand: Direct

Name: mlc [H]

Synonym: t1409

Alternate gene names: 29141862

Gene position: 1453593-1454753 (Clockwise)

Preceding gene: 29141861

Following gene: 29141863

Centisome position: 30.33

GC content: 51.68

Gene sequence:

>1161_bases
GTGTATCGCCTGATTGATCAGCTCGGACCGGTATCGCGAATTGACCTGTCTCGTCTGGCGCAGTTGGCGCCTGCCAGTAT
TACGAAAATTGTTCGCGAAATGCTGGAAGCGCATTTGGTTCAGGAACTTGAAATTAAAGAGGCGGGCAGTCGTGGACGTC
CCGCCGTCGGGCTGATGGTGGAAACGGAAGCCTGGCACTATTTATCTATTCGTATTAGCCGTGGCGAAATTTTCCTTGCA
CTGCGCGATCTTAGCAGCAAACTGGTGGTAGAAGAGTGTCTGGCGCTGCCGTTAAACGAAGCTACGCCGTTGCTTGAGCG
AATTATTACGCACGTTGATCGGTTTTTTACCCGCCATCAGCAGAAACTGGAGCGTTTGACCTCCATTGCCATTACGTTAC
CGGGCATTATCGATACCGAAAACGGTGTTGTGCACCGGATGCCGTATTACGAAGATGTCAAAGAGATGCCTTTGGGAGAT
GCGCTGGAGCGGCACACCGGCGTACCGGTTTACATTCAGCATGATATTAGCGCCTGGACGATGGCAGAGGCGCTTTTTGG
CGCCTCACGCGGCGCGCGCGACGTTATCCAGGTGGTGATTGATCATAATGTGGGGGCGGGCGTTATCACCGACGGTCATT
TGCTTCATGCGGGCAGTAGCAGTCTGGTAGAGATTGGGCATACCCAGGTCAATCCTTATGGTAAGCGCTGTTATTGCGGT
AATCATGGCTGTCTGGAGACCATCGCCAGCGTCGATAGTGTGCTGGAACTTACGCAGCTTCGGCTTAATCAGTCGATAAG
TTCAATGTTGCATGGCCAGCCGTTAACGGTAGATTCGCTGTGTCAGGCGGCGATGCAGGGAGATCTATTAGCAAAAGATA
TTATTAGCGGCGTTGGCGCGCATGTCGGACGCATTCTGGCTATCATGGTGAATTTATTTAATCCGCAAAAAATTCTTATT
GGTTCGCCGCTAAGTAAAGCGGCTGATATTCTTTTTCCGGCCATTGCTGACAGTATCCGTCAGCAGGCGCTGCCCGCCTA
CAGTAGGAATACGGTAGTGGAAAGCACGCAGTTTACCAACCAGAGCACGATGGCCGGGGCGGCGTTGGTAAAAGACGCGA
TGTATAACGGCTCTTTGTTGATTCGTCTATTACAGGGTTAA

Upstream 100 bases:

>100_bases
CACAGATTATTTCGGAGCGCGAAAATAAAGGGAGTGAGCGGTGGTTGCTGATAGTCAGCCTGGGCATATCGATCAAATTA
AGCAGACCAATGCTGGCGCA

Downstream 100 bases:

>100_bases
CATTTTTTAACTGTTGTACAAAAATTTGCGCTAACTCAAGCTGATTGCCCTTGCCATATCTTAGACTTTCTCCACTGTAT
TATTTTCCTGGCTTATATTT

Product: regulatory protein

Products: NA

Alternate protein names: Making large colonies protein [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MYRLIDQLGPVSRIDLSRLAQLAPASITKIVREMLEAHLVQELEIKEAGSRGRPAVGLMVETEAWHYLSIRISRGEIFLA
LRDLSSKLVVEECLALPLNEATPLLERIITHVDRFFTRHQQKLERLTSIAITLPGIIDTENGVVHRMPYYEDVKEMPLGD
ALERHTGVPVYIQHDISAWTMAEALFGASRGARDVIQVVIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVNPYGKRCYCG
NHGCLETIASVDSVLELTQLRLNQSISSMLHGQPLTVDSLCQAAMQGDLLAKDIISGVGAHVGRILAIMVNLFNPQKILI
GSPLSKAADILFPAIADSIRQQALPAYSRNTVVESTQFTNQSTMAGAALVKDAMYNGSLLIRLLQG

Sequences:

>Translated_386_residues
MYRLIDQLGPVSRIDLSRLAQLAPASITKIVREMLEAHLVQELEIKEAGSRGRPAVGLMVETEAWHYLSIRISRGEIFLA
LRDLSSKLVVEECLALPLNEATPLLERIITHVDRFFTRHQQKLERLTSIAITLPGIIDTENGVVHRMPYYEDVKEMPLGD
ALERHTGVPVYIQHDISAWTMAEALFGASRGARDVIQVVIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVNPYGKRCYCG
NHGCLETIASVDSVLELTQLRLNQSISSMLHGQPLTVDSLCQAAMQGDLLAKDIISGVGAHVGRILAIMVNLFNPQKILI
GSPLSKAADILFPAIADSIRQQALPAYSRNTVVESTQFTNQSTMAGAALVKDAMYNGSLLIRLLQG
>Mature_386_residues
MYRLIDQLGPVSRIDLSRLAQLAPASITKIVREMLEAHLVQELEIKEAGSRGRPAVGLMVETEAWHYLSIRISRGEIFLA
LRDLSSKLVVEECLALPLNEATPLLERIITHVDRFFTRHQQKLERLTSIAITLPGIIDTENGVVHRMPYYEDVKEMPLGD
ALERHTGVPVYIQHDISAWTMAEALFGASRGARDVIQVVIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVNPYGKRCYCG
NHGCLETIASVDSVLELTQLRLNQSISSMLHGQPLTVDSLCQAAMQGDLLAKDIISGVGAHVGRILAIMVNLFNPQKILI
GSPLSKAADILFPAIADSIRQQALPAYSRNTVVESTQFTNQSTMAGAALVKDAMYNGSLLIRLLQG

Specific function: Transcriptional repressor that regulates the expression of proteins that are part of the phosphotransferase system for sugar uptake. Regulates the expression of malT [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI190014632, Length=227, Percent_Identity=26.431718061674, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI298566325, Length=227, Percent_Identity=26.431718061674, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI4885285, Length=227, Percent_Identity=26.431718061674, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI298566315, Length=227, Percent_Identity=26.431718061674, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1787878, Length=386, Percent_Identity=90.4145077720207, Blast_Score=723, Evalue=0.0,
Organism=Escherichia coli, GI1786891, Length=385, Percent_Identity=39.7402597402597, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI87082127, Length=309, Percent_Identity=24.2718446601942, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI87081733, Length=302, Percent_Identity=24.5033112582781, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: NA

Molecular weight: Translated: 42186; Mature: 42186

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRLIDQLGPVSRIDLSRLAQLAPASITKIVREMLEAHLVQELEIKEAGSRGRPAVGLMV
CCCHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
ETEAWHYLSIRISRGEIFLALRDLSSKLVVEECLALPLNEATPLLERIITHVDRFFTRHQ
ECCCEEEEEEEEECCEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
QKLERLTSIAITLPGIIDTENGVVHRMPYYEDVKEMPLGDALERHTGVPVYIQHDISAWT
HHHHHHHHHHEECCCEEECCCCCEEECCCHHHHHHCCCCHHHHHHCCCCEEEECCCHHHH
MAEALFGASRGARDVIQVVIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVNPYGKRCYCG
HHHHHHHCCCCHHHHHHHHHHCCCCCCEEECCEEEECCCCCCEECCCCCCCCCCCEEEEC
NHGCLETIASVDSVLELTQLRLNQSISSMLHGQPLTVDSLCQAAMQGDLLAKDIISGVGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
HVGRILAIMVNLFNPQKILIGSPLSKAADILFPAIADSIRQQALPAYSRNTVVESTQFTN
HHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHCCC
QSTMAGAALVKDAMYNGSLLIRLLQG
CHHHHHHHHHHHHHCCCHHHHEEECC
>Mature Secondary Structure
MYRLIDQLGPVSRIDLSRLAQLAPASITKIVREMLEAHLVQELEIKEAGSRGRPAVGLMV
CCCHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
ETEAWHYLSIRISRGEIFLALRDLSSKLVVEECLALPLNEATPLLERIITHVDRFFTRHQ
ECCCEEEEEEEEECCEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
QKLERLTSIAITLPGIIDTENGVVHRMPYYEDVKEMPLGDALERHTGVPVYIQHDISAWT
HHHHHHHHHHEECCCEEECCCCCEEECCCHHHHHHCCCCHHHHHHCCCCEEEECCCHHHH
MAEALFGASRGARDVIQVVIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVNPYGKRCYCG
HHHHHHHCCCCHHHHHHHHHHCCCCCCEEECCEEEECCCCCCEECCCCCCCCCCCEEEEC
NHGCLETIASVDSVLELTQLRLNQSISSMLHGQPLTVDSLCQAAMQGDLLAKDIISGVGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
HVGRILAIMVNLFNPQKILIGSPLSKAADILFPAIADSIRQQALPAYSRNTVVESTQFTN
HHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHCCC
QSTMAGAALVKDAMYNGSLLIRLLQG
CHHHHHHHHHHHHHCCCHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7766024; 9097039; 9278503 [H]