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The map label for this gene is mepA [H]

Identifier: 29140997

GI number: 29140997

Start: 555155

End: 555979

Strand: Direct

Name: mepA [H]

Synonym: t0481

Alternate gene names: 29140997

Gene position: 555155-555979 (Clockwise)

Preceding gene: 29140996

Following gene: 29140998

Centisome position: 11.59

GC content: 58.67

Gene sequence:

>825_bases
ATGAAAAAAACCGCGATTGCGCTGCTGGCATGGTTTGTCAGTAGCGCCAGCCTGGCGGCGACGCCGTGGCAGAAAATAAC
CCATCCTGTCCCCGGCGCCGCCCAGTCTATCGGTAGCTTTGCCAACGGATGCATCATTGGCGCCGACACGTTGCCGGTAC
AGTCCGATAATTATCAGGTGATGCGCACCGATCAGCGCCGTTATTTCGGCCACCCGGATCTGGTCATGTTTATCCAGCGG
TTGAGTCATCAGGCGCAGCAACGGGGGCTCGGAACCGTCCTGATAGGCGACATGGGGATGCCTGCCGGAGGCCGCTTTAA
TGGCGGACACGCCAGTCATCAGACCGGGCTTGATGTGGATATTTTCTTGCAGTTGCCGAAAACGCGCTGGAGCCAGGCGC
AGCTATTGCGCCCGCAGGCGTTAGATCTGGTGTCCCGCGACGGTAAACATGTCGTGCCGTCGCGCTGGTCGTCGGATATC
GCCAGTCTGATCAAACTGGCGGCACAAGACAATGACGTCACCCGTATTTTCGTCAATCCGGCTATTAAACAACAGCTTTG
CCTCGATGCCGGAAGCGATCGTGACTGGCTACGTAAAGTACGCCCCTGGTTCCAGCATCGCGCGCATATGCACGTGCGTT
TACGCTGCCCTGCCGACAGCCTGGAGTGCGAAGATCAACCTTTACCCCCGCCGGGCGATGGATGCGGCGCTGAACTGCAA
AGCTGGTTCGAACCGCCAAAACCTGGCACCACAAAGCCTGAGAAGAAGACACCGCCGCCGTTGCCGCCTTCCTGCCAGGC
GCTACTGGATGAGCATGTACTCTGA

Upstream 100 bases:

>100_bases
GATCGCAGAAGCCATGCTGGCGATCGTACTGATGGATCACCTGCTGCGCCATCGGGCACAGAATGCGGATGTAAAGACAG
AGATTCCACGCTGGTAAGAA

Downstream 100 bases:

>100_bases
TGGACAATTTTTATGATCTGTTTATGGTCTCCCCGCTGCTGCTGGTGGTGCTGTTTTTTGTCGCCGTACTGGCAGGATTT
ATCGATTCTATCGCCGGAGG

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: D-alanyl-D-alanine-endopeptidase; DD-endopeptidase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MKKTAIALLAWFVSSASLAATPWQKITHPVPGAAQSIGSFANGCIIGADTLPVQSDNYQVMRTDQRRYFGHPDLVMFIQR
LSHQAQQRGLGTVLIGDMGMPAGGRFNGGHASHQTGLDVDIFLQLPKTRWSQAQLLRPQALDLVSRDGKHVVPSRWSSDI
ASLIKLAAQDNDVTRIFVNPAIKQQLCLDAGSDRDWLRKVRPWFQHRAHMHVRLRCPADSLECEDQPLPPPGDGCGAELQ
SWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVL

Sequences:

>Translated_274_residues
MKKTAIALLAWFVSSASLAATPWQKITHPVPGAAQSIGSFANGCIIGADTLPVQSDNYQVMRTDQRRYFGHPDLVMFIQR
LSHQAQQRGLGTVLIGDMGMPAGGRFNGGHASHQTGLDVDIFLQLPKTRWSQAQLLRPQALDLVSRDGKHVVPSRWSSDI
ASLIKLAAQDNDVTRIFVNPAIKQQLCLDAGSDRDWLRKVRPWFQHRAHMHVRLRCPADSLECEDQPLPPPGDGCGAELQ
SWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVL
>Mature_274_residues
MKKTAIALLAWFVSSASLAATPWQKITHPVPGAAQSIGSFANGCIIGADTLPVQSDNYQVMRTDQRRYFGHPDLVMFIQR
LSHQAQQRGLGTVLIGDMGMPAGGRFNGGHASHQTGLDVDIFLQLPKTRWSQAQLLRPQALDLVSRDGKHVVPSRWSSDI
ASLIKLAAQDNDVTRIFVNPAIKQQLCLDAGSDRDWLRKVRPWFQHRAHMHVRLRCPADSLECEDQPLPPPGDGCGAELQ
SWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVL

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=274, Percent_Identity=89.051094890511, Blast_Score=487, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 30235; Mature: 30235

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTAIALLAWFVSSASLAATPWQKITHPVPGAAQSIGSFANGCIIGADTLPVQSDNYQV
CCHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHCCEEECCCCCCCCCCCEEE
MRTDQRRYFGHPDLVMFIQRLSHQAQQRGLGTVLIGDMGMPAGGRFNGGHASHQTGLDVD
EECCCHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEE
IFLQLPKTRWSQAQLLRPQALDLVSRDGKHVVPSRWSSDIASLIKLAAQDNDVTRIFVNP
EEEECCCCCCCHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECH
AIKQQLCLDAGSDRDWLRKVRPWFQHRAHMHVRLRCPADSLECEDQPLPPPGDGCGAELQ
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
SWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVL
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKKTAIALLAWFVSSASLAATPWQKITHPVPGAAQSIGSFANGCIIGADTLPVQSDNYQV
CCHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHCCEEECCCCCCCCCCCEEE
MRTDQRRYFGHPDLVMFIQRLSHQAQQRGLGTVLIGDMGMPAGGRFNGGHASHQTGLDVD
EECCCHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEE
IFLQLPKTRWSQAQLLRPQALDLVSRDGKHVVPSRWSSDIASLIKLAAQDNDVTRIFVNP
EEEECCCCCCCHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECH
AIKQQLCLDAGSDRDWLRKVRPWFQHRAHMHVRLRCPADSLECEDQPLPPPGDGCGAELQ
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
SWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVL
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA