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The map label for this gene is leuO [H]

Identifier: 29140660

GI number: 29140660

Start: 135975

End: 136919

Strand: Direct

Name: leuO [H]

Synonym: t0119

Alternate gene names: 29140660

Gene position: 135975-136919 (Clockwise)

Preceding gene: 29140650

Following gene: 29140661

Centisome position: 2.84

GC content: 48.36

Gene sequence:

>945_bases
ATGCCAGAGGTCAAAACCGAAAAGCCGCATCTTTTAGATATGGGCAAACCACAGCTTCGCATGGTTGATTTGAACCTATT
GACCGTGTTCGATGCGGTAATGCAAGAGCAGAATATTACGCGCGCCGCCCACACGCTGGGAATGTCGCAGCCTGCGGTCA
GTAACGCCGTAGCGCGTCTGAAGGTTATGTTTAATGACGAACTTTTTGTTCGATATGGACGAGGAATTCAGCCGACTGCC
CGTGCATTTCAGTTATTTGGTTCAGTCCGTCAGGCGTTGCAATTGGTGCAAAATGAATTGCCGGGATCGGGGTTTGAGCC
GACCAGCAGCGAACGTGTATTCAATCTTTGCGTGTGCAGTCCGCTGGATAATATCCTGACGTCACAGATTTATAATCGTG
TAGAAAAAATTGCGCCAAATATTCATGTCGTTTTTAAAGCGTCGTTGAATCAGAATACTGAGCATCAGTTACGCTATCAG
GAAACCGAGTTCGTTATTAGTTATGAAGAATTCCGTCGTCCTGAGTTTACCAGCGTACCGCTATTTAAAGATGAAATGGT
TTTAGTCGCCAGCCGAAAACACCCGCGTATTAGCGGCCCGCTACTGGAAGGCGATGTTTATAATGAACAACATGCGGTTG
TTTCTCTCGATCGTTATGCGTCATTTAGTCGGCCGTGGTATGACACGCCGGATAAACAGTCGAGCGTGGCTTATCAGGGC
ATGGCGCTTATCAGCGTTCTGAACGTGGTTTCGCAGACGCATTTGGTCGCTATTGCCCCGCGCTGGCTGGCGGAAGAGTT
TGCGGAGTCGCTGGAGCTGCAAATACTGCCGTTGCCTTTAAAACTGAATAGCCGGACATGCTACCTTTCCTGGCATGAAG
CGGCTGGGCGTGATAAAGGGCATCAATGGATGGAAGATTTATTAGTCTCTGTTTGTAAGCGATAA

Upstream 100 bases:

>100_bases
TCCCTTTTTCTTATTTTATATGCATGGTAAATCATATTTTCAGGAATTATTTCTGCCTTCAGCCAGAAAAAGGGAGTTAA
GCGTGACAGTGGAGTTAAAT

Downstream 100 bases:

>100_bases
AACCGGGCAGAATAAATCAGAAACAAATTCTGGTTTATTCTGCTTTTTAGCGTATGTCAAAAATTTTATGCTGAATTAAC
GGTGAGCGATTCTTTTCGTC

Product: leucine transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MPEVKTEKPHLLDMGKPQLRMVDLNLLTVFDAVMQEQNITRAAHTLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTA
RAFQLFGSVRQALQLVQNELPGSGFEPTSSERVFNLCVCSPLDNILTSQIYNRVEKIAPNIHVVFKASLNQNTEHQLRYQ
ETEFVISYEEFRRPEFTSVPLFKDEMVLVASRKHPRISGPLLEGDVYNEQHAVVSLDRYASFSRPWYDTPDKQSSVAYQG
MALISVLNVVSQTHLVAIAPRWLAEEFAESLELQILPLPLKLNSRTCYLSWHEAAGRDKGHQWMEDLLVSVCKR

Sequences:

>Translated_314_residues
MPEVKTEKPHLLDMGKPQLRMVDLNLLTVFDAVMQEQNITRAAHTLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTA
RAFQLFGSVRQALQLVQNELPGSGFEPTSSERVFNLCVCSPLDNILTSQIYNRVEKIAPNIHVVFKASLNQNTEHQLRYQ
ETEFVISYEEFRRPEFTSVPLFKDEMVLVASRKHPRISGPLLEGDVYNEQHAVVSLDRYASFSRPWYDTPDKQSSVAYQG
MALISVLNVVSQTHLVAIAPRWLAEEFAESLELQILPLPLKLNSRTCYLSWHEAAGRDKGHQWMEDLLVSVCKR
>Mature_313_residues
PEVKTEKPHLLDMGKPQLRMVDLNLLTVFDAVMQEQNITRAAHTLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTAR
AFQLFGSVRQALQLVQNELPGSGFEPTSSERVFNLCVCSPLDNILTSQIYNRVEKIAPNIHVVFKASLNQNTEHQLRYQE
TEFVISYEEFRRPEFTSVPLFKDEMVLVASRKHPRISGPLLEGDVYNEQHAVVSLDRYASFSRPWYDTPDKQSSVAYQGM
ALISVLNVVSQTHLVAIAPRWLAEEFAESLELQILPLPLKLNSRTCYLSWHEAAGRDKGHQWMEDLLVSVCKR

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=314, Percent_Identity=87.2611464968153, Blast_Score=584, Evalue=1e-168,
Organism=Escherichia coli, GI87081766, Length=289, Percent_Identity=28.3737024221453, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1786820, Length=289, Percent_Identity=23.8754325259516, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35758; Mature: 35627

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEVKTEKPHLLDMGKPQLRMVDLNLLTVFDAVMQEQNITRAAHTLGMSQPAVSNAVARL
CCCCCCCCCCEECCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHE
KVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPGSGFEPTSSERVFNLCVCS
EEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
PLDNILTSQIYNRVEKIAPNIHVVFKASLNQNTEHQLRYQETEFVISYEEFRRPEFTSVP
CHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHEEECHHEEEEHHHHCCCCCCCCC
LFKDEMVLVASRKHPRISGPLLEGDVYNEQHAVVSLDRYASFSRPWYDTPDKQSSVAYQG
CCCCCEEEEECCCCCCCCCCEECCCCCCCCCCEEEHHHHHCCCCCCCCCCCCCCHHHHHH
MALISVLNVVSQTHLVAIAPRWLAEEFAESLELQILPLPLKLNSRTCYLSWHEAAGRDKG
HHHHHHHHHHHHHHEEEECHHHHHHHHHHHCCEEEEEEEEEECCCEEEEEEHHHCCCCCH
HQWMEDLLVSVCKR
HHHHHHHHHHHHCC
>Mature Secondary Structure 
PEVKTEKPHLLDMGKPQLRMVDLNLLTVFDAVMQEQNITRAAHTLGMSQPAVSNAVARL
CCCCCCCCCEECCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHE
KVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPGSGFEPTSSERVFNLCVCS
EEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
PLDNILTSQIYNRVEKIAPNIHVVFKASLNQNTEHQLRYQETEFVISYEEFRRPEFTSVP
CHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHEEECHHEEEEHHHHCCCCCCCCC
LFKDEMVLVASRKHPRISGPLLEGDVYNEQHAVVSLDRYASFSRPWYDTPDKQSSVAYQG
CCCCCEEEEECCCCCCCCCCEECCCCCCCCCCEEEHHHHHCCCCCCCCCCCCCCHHHHHH
MALISVLNVVSQTHLVAIAPRWLAEEFAESLELQILPLPLKLNSRTCYLSWHEAAGRDKG
HHHHHHHHHHHHHHEEEECHHHHHHHHHHHCCEEEEEEEEEECCCEEEEEEHHHCCCCCH
HQWMEDLLVSVCKR
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3519576 [H]