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The map label for this gene is ilvI [H]

Identifier: 29140661

GI number: 29140661

Start: 137241

End: 138965

Strand: Direct

Name: ilvI [H]

Synonym: t0120

Alternate gene names: 29140661

Gene position: 137241-138965 (Clockwise)

Preceding gene: 29140660

Following gene: 29140662

Centisome position: 2.86

GC content: 54.55

Gene sequence:

>1725_bases
GTGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCTTATCGATCAAGGCGTAAAGCAGGTGTTCGGCTATCCTGG
AGGCGCAGTGCTTGATATTTATGATGCGCTACATACCGTTGGAGGGATCGATCATGTGCTGGTGCGTCATGAGCAGGCCG
CGGTGCATATGGCGGATGGCCTGGCGCGAGCCACTGGCGACGTTGGCGTGGTGCTGGTGACTTCCGGACCGGGAGCGACC
AATGCGATTACAGGGATTGCTACGGCCTATATGGATTCCATTCCGCTGGTGATTCTTTCCGGCCAGGTCGCCACCTCATT
GATTGGCTATGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCCCGACCGGTGGTCAAACATAGCTTCCTGGTCAAAC
AGACGGAAGATATTCCCCTGGTGCTTAAAAAGGCCTTCTGGCTGGCGGCAAGCGGACGTCCTGGGCCGGTGGTCGTGGAT
CTGCCGAAAGATATTTTGAATCCGGCGAAAAAAATGCCGTATGTCTGGCCGGAAACGGTCAGTATGCGCTCTTACAATCC
CACAACATCAGGACATAAAGGACAAATTAAGCGTGCCTTGCAAACGCTGGCGTCGGCAAAAAAGCCGGTGGTCTATGTTG
GGGGAGGGGCGATAAGCGCGGCCTGCTATGCGCCGCTGCGCCACATCATTGAAACCTTTAACCTGCCGGTGGTCTCTTCA
TTAATGGGACTGGGCGCGTTTCCTGCTACTCATCGCCAGTCGTTAGGAATGCTGGGAATGCATGGCACTTATGAAGCCAA
TATGACCATGCATAACGCCGACGTGATTTTCGCTGTTGGCGTACGATTTGACGACCGCACGACGAATAATCTCGCTAAAT
ATTGTCCGAACGCGACGGTATTACACATCGACATTGACCCGACGTCCATATCCAAAACGGTTAACGCTGATATCCCGGTG
GTGGGCGATGCACGCCTGGTACTGGAACAAATGCTGGAGCTACTGGCGCAGGATGCGCCGTCTCAGCCGCAGGATGATAT
TCGCGACTGGTGGCAGCAGATAGAGAGCTGGCGCGCCCGTCAGTGCCTGAAATATGACGCGGAAAGCGAAAGCATTAAAC
CCCAGGCGGTGATTGAAACTCTCTGGCGTCTGACGAAGGGTGATGCGTACGTGACGTCCGATGTAGGACAACACCAGATG
TTTGCCGCCCTCTACTACCCGTTCGATAAGCCACGTCGCTGGATTAACTCTGGCGGGCTCGGCACGATGGGGTTTGGCCT
ACCGGCTGCACTGGGCGTTAAAATGGCCCTGCCGAAAGAAATGGTGGTCTGCGTGACCGGCGATGGCAGTATACAGATGA
ACATTCAGGAGCTGTCGACAGCCTTACAGTATGAACTGCCGGTACTGGTTCTGAACCTGAACAACCGTTACCTGGGGATG
GTGAAACAGTGGCAGGATATGATCTATTCCGGTCGCCATTCTCAGTCTTATATGCAGTCGTTACCGGATTTTGTGCGCCT
GGCGGAAGCATATGGCCATGTCGGGCTGCAGATTAACCGTCCGGATGAGCTGGAAAGTAAACTCAGCGAAGCCCTTGAGC
ATGTGCGTAATAACCGACTGGTGTTCGTCGATGTCACCGTTGATGGCAGCGAGCATGTCTATCCGATGCAGATTCGCGGG
GGCGGGATGGACGAAATGTGGTTAAGCAAAACGGAGAGGACCTGA

Upstream 100 bases:

>100_bases
TTCCCCATCTTTACGTCATTCCACCCAATTGCCAGGCGTGATAGCGAGCGGTTAAGGTGTCCGTCAAACCGTAATGACAG
GTATAAAGTGGAGGCAAGCC

Downstream 100 bases:

>100_bases
TGATGCGCCGGATTTTATCGGTATTACTGGAAAACGAATCTGGGGCGTTATCGCGGGTTATCGGCCTCTTTTCGCAACGC
GGATATAATATTGAAAGCCT

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADGLARATGDVGVVLVTSGPGAT
NAITGIATAYMDSIPLVILSGQVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPLVLKKAFWLAASGRPGPVVVD
LPKDILNPAKKMPYVWPETVSMRSYNPTTSGHKGQIKRALQTLASAKKPVVYVGGGAISAACYAPLRHIIETFNLPVVSS
LMGLGAFPATHRQSLGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVNADIPV
VGDARLVLEQMLELLAQDAPSQPQDDIRDWWQQIESWRARQCLKYDAESESIKPQAVIETLWRLTKGDAYVTSDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPKEMVVCVTGDGSIQMNIQELSTALQYELPVLVLNLNNRYLGM
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGLQINRPDELESKLSEALEHVRNNRLVFVDVTVDGSEHVYPMQIRG
GGMDEMWLSKTERT

Sequences:

>Translated_574_residues
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADGLARATGDVGVVLVTSGPGAT
NAITGIATAYMDSIPLVILSGQVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPLVLKKAFWLAASGRPGPVVVD
LPKDILNPAKKMPYVWPETVSMRSYNPTTSGHKGQIKRALQTLASAKKPVVYVGGGAISAACYAPLRHIIETFNLPVVSS
LMGLGAFPATHRQSLGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVNADIPV
VGDARLVLEQMLELLAQDAPSQPQDDIRDWWQQIESWRARQCLKYDAESESIKPQAVIETLWRLTKGDAYVTSDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPKEMVVCVTGDGSIQMNIQELSTALQYELPVLVLNLNNRYLGM
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGLQINRPDELESKLSEALEHVRNNRLVFVDVTVDGSEHVYPMQIRG
GGMDEMWLSKTERT
>Mature_574_residues
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADGLARATGDVGVVLVTSGPGAT
NAITGIATAYMDSIPLVILSGQVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPLVLKKAFWLAASGRPGPVVVD
LPKDILNPAKKMPYVWPETVSMRSYNPTTSGHKGQIKRALQTLASAKKPVVYVGGGAISAACYAPLRHIIETFNLPVVSS
LMGLGAFPATHRQSLGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVNADIPV
VGDARLVLEQMLELLAQDAPSQPQDDIRDWWQQIESWRARQCLKYDAESESIKPQAVIETLWRLTKGDAYVTSDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPKEMVVCVTGDGSIQMNIQELSTALQYELPVLVLNLNNRYLGM
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGLQINRPDELESKLSEALEHVRNNRLVFVDVTVDGSEHVYPMQIRG
GGMDEMWLSKTERT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=562, Percent_Identity=26.8683274021352, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI21361361, Length=586, Percent_Identity=26.7918088737201, Blast_Score=166, Evalue=5e-41,
Organism=Escherichia coli, GI87081685, Length=574, Percent_Identity=92.3344947735192, Blast_Score=1110, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=567, Percent_Identity=39.1534391534392, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1786717, Length=549, Percent_Identity=34.0619307832423, Blast_Score=311, Evalue=6e-86,
Organism=Escherichia coli, GI1787096, Length=560, Percent_Identity=28.5714285714286, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1788716, Length=556, Percent_Identity=25, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17531299, Length=561, Percent_Identity=26.9162210338681, Blast_Score=166, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17531301, Length=561, Percent_Identity=26.9162210338681, Blast_Score=166, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17542570, Length=576, Percent_Identity=26.3888888888889, Blast_Score=154, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6323755, Length=584, Percent_Identity=43.1506849315069, Blast_Score=448, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320816, Length=475, Percent_Identity=25.2631578947368, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI19922626, Length=508, Percent_Identity=28.5433070866142, Blast_Score=193, Evalue=3e-49,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62925; Mature: 62925

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LARATGDVGVVLVTSGPGATNAITGIATAYMDSIPLVILSGQVATSLIGYDAFQECDMVG
HHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCC
ISRPVVKHSFLVKQTEDIPLVLKKAFWLAASGRPGPVVVDLPKDILNPAKKMPYVWPETV
CCCHHHHHHHHHCCCCCCHHHHHHHHHEEECCCCCCEEEECCHHHCCHHHHCCCCCCCCC
SMRSYNPTTSGHKGQIKRALQTLASAKKPVVYVGGGAISAACYAPLRHIIETFNLPVVSS
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHH
LMGLGAFPATHRQSLGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATV
HHHCCCCCCHHHHCCCCCCCCCCEECCEEEECCCEEEEEEEEECCCCHHHHHHHCCCCEE
LHIDIDPTSISKTVNADIPVVGDARLVLEQMLELLAQDAPSQPQDDIRDWWQQIESWRAR
EEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
QCLKYDAESESIKPQAVIETLWRLTKGDAYVTSDVGQHQMFAALYYPFDKPRRWINSGGL
HHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHEECCCHHHHHHHCCCCC
GTMGFGLPAALGVKMALPKEMVVCVTGDGSIQMNIQELSTALQYELPVLVLNLNNRYLGM
CCCCCCCHHHHCCCEECCCEEEEEEECCCCEEEEHHHHHHHHHEECCEEEEECCCCHHHH
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGLQINRPDELESKLSEALEHVRNNRL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHCCCE
VFVDVTVDGSEHVYPMQIRGGGMDEMWLSKTERT
EEEEEEECCCCCEEEEEECCCCCCHHHHHHCCCC
>Mature Secondary Structure
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LARATGDVGVVLVTSGPGATNAITGIATAYMDSIPLVILSGQVATSLIGYDAFQECDMVG
HHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCC
ISRPVVKHSFLVKQTEDIPLVLKKAFWLAASGRPGPVVVDLPKDILNPAKKMPYVWPETV
CCCHHHHHHHHHCCCCCCHHHHHHHHHEEECCCCCCEEEECCHHHCCHHHHCCCCCCCCC
SMRSYNPTTSGHKGQIKRALQTLASAKKPVVYVGGGAISAACYAPLRHIIETFNLPVVSS
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHH
LMGLGAFPATHRQSLGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATV
HHHCCCCCCHHHHCCCCCCCCCCEECCEEEECCCEEEEEEEEECCCCHHHHHHHCCCCEE
LHIDIDPTSISKTVNADIPVVGDARLVLEQMLELLAQDAPSQPQDDIRDWWQQIESWRAR
EEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
QCLKYDAESESIKPQAVIETLWRLTKGDAYVTSDVGQHQMFAALYYPFDKPRRWINSGGL
HHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHEECCCHHHHHHHCCCCC
GTMGFGLPAALGVKMALPKEMVVCVTGDGSIQMNIQELSTALQYELPVLVLNLNNRYLGM
CCCCCCCHHHHCCCEECCCEEEEEEECCCCEEEEHHHHHHHHHEECCEEEEECCCCHHHH
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGLQINRPDELESKLSEALEHVRNNRL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHCCCE
VFVDVTVDGSEHVYPMQIRGGGMDEMWLSKTERT
EEEEEEECCCCCEEEEEECCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]