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The map label for this gene is citA [H]

Identifier: 29140597

GI number: 29140597

Start: 61791

End: 63410

Strand: Reverse

Name: citA [H]

Synonym: t0055

Alternate gene names: 29140597

Gene position: 63410-61791 (Counterclockwise)

Preceding gene: 29140598

Following gene: 29140596

Centisome position: 1.32

GC content: 47.84

Gene sequence:

>1620_bases
ATGTTTATACGGGAAATTACACACTGGTTTGCCAGACGCTCGTTTCAAAACCGGATATTTCTACTGATCCTGTTTACATC
CACCATCGTGATGCTGGCAATCACCTGGTATCTGACCGATATCACGAAAGAGCGCCTGCATTATCAGGTGGGCCAACGTG
CGCTTATTCAAGCAATGCAAATATCCGCTATGCCAGAATTAGTTGAAGCCGTAGAGGCGCATGATTTGTCACGCATCAAA
GCGCTGATTGACCCAATGCGCTCGTTTTCGGACGCGACTTACATTACCGTTGGCAACGAAAAAGGTCAGCGACTGTATCA
CGTCAACCCGGATGAGATCGGCAAATATATGGAGGGCGGCGATAGCGACGACGCGCTGTATAATGCTAAAAGCTACGTAT
CGGTGCGCAAAGGTTCATTAGGCTCTTCCTTGCGTGGTAAATCCCCGATTCAGGACAGTACCGGTAAAGTCATCGGTATT
GTTTCCGTCGGCTATACTCTGGAACAGTTGGAGAGCTGGCTTAACTTACAAATCAGCTCGTTGCTGTTCCCGATGGCGAT
ACTGTTGCTGGTGCTGTTGTACTGCGCCCGCCGCTTCTCGCTGCATATTAAAAAGCAGATGCTTAACATGGAGCCGCAGC
AGCTTTCACAACTATTAATTCAGCAAAGCGTGTTATTTGAGTCGGTATTTGAAGGGTTGATCGCCATTGACTCCCATCAC
CGTATTACCGCCATTAATCAAACGGCTCGCCGCTTACTGAATCTGAGCCAACCGGAGTCTGAACTCATTGGCTATGCAAT
CCACCATATCGTGTTGCCGGAGGCCTTCTTTTACGATGCGCCGCAAAAAAACAAAAAAGATGAGATCGTCACTTTTAACC
AAATGAAAGTCATCGCCAGTCGCATGGCGGTCATTATTGACAACCAGCCTCAGGGCTGGGTTATTAGCTTTCGCAACAAA
GACGATATCAATACACTCAACCTGCAGTTAAGTCAGGTGCGGCAATACGCCGATAACCTGCGCGCCGTGCAGCATGAGCA
TCGCAACCTTATTTCCACCATTGCCGGACTGCTGTTCCTGAAGCGCTACGATAATGCGCTGGCGCTGATTCAGCAGCAGT
CGGAAAGCCACCAAAAAGTACTCGATTTTATTTCCCATAATTTCCGCGACCACCACCTTGCGGGGCTATTAATCGGTAAA
TATTATCGGGCAAAAGAGCTGGGGCTGGAGCTGATTTTCGATCCTTCAAGCTTTGTTGAACACCTGCCGTCATCGCTCTC
GCATAATGAATGGATTTCGATTGTTGGCAATTTATTGGACAACGCCTATAACGCCACCCTGCTCAATCGCGAAGGCTCGC
GACAGATTGAGTGCCTGATCAATAGCGAAGGTAATGAAGTCATTATTGAAGTTGCCGATCAGGGATGTGGTATTGACGAA
AATATCCGCCAGCATATTTTCGAGCGTGGAGTGACCACCCACAACAACGGCGATCACGGAATTGGATTATGGTTGGTGCA
CAGCTACGTCAAGCAGGCCAATGGCGACATTATCGTCGATAACAATAATTCTTTTGGCACCATTTTTACCCTCTATATCC
CTTTTACCAGAGAAGAATAA

Upstream 100 bases:

>100_bases
CATCCAAATTTTTTCTGAAAGAAAAGTAAGTATGAACTGGTTCAGGTTCCGGTGATGCCGAATGCAAGAAAATACCGTAA
TTAAATATCTAAATACCAAT

Downstream 100 bases:

>100_bases
CATGGATAGCATTACGACACTCATCGTTGAAGACGAACCGATGCTAGCGGAAATCCTGGTGGACACCATCAAGCTTTTTC
CTCAGTTTTCAATCGTCGGT

Product: sensor kinase CitA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MFIREITHWFARRSFQNRIFLLILFTSTIVMLAITWYLTDITKERLHYQVGQRALIQAMQISAMPELVEAVEAHDLSRIK
ALIDPMRSFSDATYITVGNEKGQRLYHVNPDEIGKYMEGGDSDDALYNAKSYVSVRKGSLGSSLRGKSPIQDSTGKVIGI
VSVGYTLEQLESWLNLQISSLLFPMAILLLVLLYCARRFSLHIKKQMLNMEPQQLSQLLIQQSVLFESVFEGLIAIDSHH
RITAINQTARRLLNLSQPESELIGYAIHHIVLPEAFFYDAPQKNKKDEIVTFNQMKVIASRMAVIIDNQPQGWVISFRNK
DDINTLNLQLSQVRQYADNLRAVQHEHRNLISTIAGLLFLKRYDNALALIQQQSESHQKVLDFISHNFRDHHLAGLLIGK
YYRAKELGLELIFDPSSFVEHLPSSLSHNEWISIVGNLLDNAYNATLLNREGSRQIECLINSEGNEVIIEVADQGCGIDE
NIRQHIFERGVTTHNNGDHGIGLWLVHSYVKQANGDIIVDNNNSFGTIFTLYIPFTREE

Sequences:

>Translated_539_residues
MFIREITHWFARRSFQNRIFLLILFTSTIVMLAITWYLTDITKERLHYQVGQRALIQAMQISAMPELVEAVEAHDLSRIK
ALIDPMRSFSDATYITVGNEKGQRLYHVNPDEIGKYMEGGDSDDALYNAKSYVSVRKGSLGSSLRGKSPIQDSTGKVIGI
VSVGYTLEQLESWLNLQISSLLFPMAILLLVLLYCARRFSLHIKKQMLNMEPQQLSQLLIQQSVLFESVFEGLIAIDSHH
RITAINQTARRLLNLSQPESELIGYAIHHIVLPEAFFYDAPQKNKKDEIVTFNQMKVIASRMAVIIDNQPQGWVISFRNK
DDINTLNLQLSQVRQYADNLRAVQHEHRNLISTIAGLLFLKRYDNALALIQQQSESHQKVLDFISHNFRDHHLAGLLIGK
YYRAKELGLELIFDPSSFVEHLPSSLSHNEWISIVGNLLDNAYNATLLNREGSRQIECLINSEGNEVIIEVADQGCGIDE
NIRQHIFERGVTTHNNGDHGIGLWLVHSYVKQANGDIIVDNNNSFGTIFTLYIPFTREE
>Mature_539_residues
MFIREITHWFARRSFQNRIFLLILFTSTIVMLAITWYLTDITKERLHYQVGQRALIQAMQISAMPELVEAVEAHDLSRIK
ALIDPMRSFSDATYITVGNEKGQRLYHVNPDEIGKYMEGGDSDDALYNAKSYVSVRKGSLGSSLRGKSPIQDSTGKVIGI
VSVGYTLEQLESWLNLQISSLLFPMAILLLVLLYCARRFSLHIKKQMLNMEPQQLSQLLIQQSVLFESVFEGLIAIDSHH
RITAINQTARRLLNLSQPESELIGYAIHHIVLPEAFFYDAPQKNKKDEIVTFNQMKVIASRMAVIIDNQPQGWVISFRNK
DDINTLNLQLSQVRQYADNLRAVQHEHRNLISTIAGLLFLKRYDNALALIQQQSESHQKVLDFISHNFRDHHLAGLLIGK
YYRAKELGLELIFDPSSFVEHLPSSLSHNEWISIVGNLLDNAYNATLLNREGSRQIECLINSEGNEVIIEVADQGCGIDE
NIRQHIFERGVTTHNNGDHGIGLWLVHSYVKQANGDIIVDNNNSFGTIFTLYIPFTREE

Specific function: Member of the two-component regulatory system CitA/CitB. Probably activates CitB by phosphorylation. The periplasmic domain binds H-citrate(2-), which is essential for induction of the citrate-fermentation genes [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=532, Percent_Identity=42.1052631578947, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1790567, Length=530, Percent_Identity=29.0566037735849, Blast_Score=182, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 61430; Mature: 61430

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIREITHWFARRSFQNRIFLLILFTSTIVMLAITWYLTDITKERLHYQVGQRALIQAMQ
CCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISAMPELVEAVEAHDLSRIKALIDPMRSFSDATYITVGNEKGQRLYHVNPDEIGKYMEGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCHHHHHHHHCCC
DSDDALYNAKSYVSVRKGSLGSSLRGKSPIQDSTGKVIGIVSVGYTLEQLESWLNLQISS
CCCCHHHCHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
LLFPMAILLLVLLYCARRFSLHIKKQMLNMEPQQLSQLLIQQSVLFESVFEGLIAIDSHH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCC
RITAINQTARRLLNLSQPESELIGYAIHHIVLPEAFFYDAPQKNKKDEIVTFNQMKVIAS
EEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCEEEHHHHHHHHH
RMAVIIDNQPQGWVISFRNKDDINTLNLQLSQVRQYADNLRAVQHEHRNLISTIAGLLFL
HHEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRYDNALALIQQQSESHQKVLDFISHNFRDHHLAGLLIGKYYRAKELGLELIFDPSSFVE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEECCHHHHH
HLPSSLSHNEWISIVGNLLDNAYNATLLNREGSRQIECLINSEGNEVIIEVADQGCGIDE
HCHHHCCCHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEECCCCEEEEEEECCCCCCCH
NIRQHIFERGVTTHNNGDHGIGLWLVHSYVKQANGDIIVDNNNSFGTIFTLYIPFTREE
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEEEECCCC
>Mature Secondary Structure
MFIREITHWFARRSFQNRIFLLILFTSTIVMLAITWYLTDITKERLHYQVGQRALIQAMQ
CCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISAMPELVEAVEAHDLSRIKALIDPMRSFSDATYITVGNEKGQRLYHVNPDEIGKYMEGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCHHHHHHHHCCC
DSDDALYNAKSYVSVRKGSLGSSLRGKSPIQDSTGKVIGIVSVGYTLEQLESWLNLQISS
CCCCHHHCHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
LLFPMAILLLVLLYCARRFSLHIKKQMLNMEPQQLSQLLIQQSVLFESVFEGLIAIDSHH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCC
RITAINQTARRLLNLSQPESELIGYAIHHIVLPEAFFYDAPQKNKKDEIVTFNQMKVIAS
EEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCEEEHHHHHHHHH
RMAVIIDNQPQGWVISFRNKDDINTLNLQLSQVRQYADNLRAVQHEHRNLISTIAGLLFL
HHEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRYDNALALIQQQSESHQKVLDFISHNFRDHHLAGLLIGKYYRAKELGLELIFDPSSFVE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEECCHHHHH
HLPSSLSHNEWISIVGNLLDNAYNATLLNREGSRQIECLINSEGNEVIIEVADQGCGIDE
HCHHHCCCHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEECCCCEEEEEEECCCCCCCH
NIRQHIFERGVTTHNNGDHGIGLWLVHSYVKQANGDIIVDNNNSFGTIFTLYIPFTREE
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8748036; 10447894 [H]