Click here to switch to the map view.

The map label for this gene is citB [H]

Identifier: 29140596

GI number: 29140596

Start: 61103

End: 61789

Strand: Reverse

Name: citB [H]

Synonym: t0054

Alternate gene names: 29140596

Gene position: 61789-61103 (Counterclockwise)

Preceding gene: 29140597

Following gene: 29140587

Centisome position: 1.29

GC content: 48.47

Gene sequence:

>687_bases
ATGGATAGCATTACGACACTCATCGTTGAAGACGAACCGATGCTAGCGGAAATCCTGGTGGACACCATCAAGCTTTTTCC
TCAGTTTTCAATCGTCGGTATCGCAGACAAGCTGGAAAGTGCGAAGAAGCAAATACGCCTCTATCAACCACAGTTAATTC
TATTGGATAATTTTTTACCCGATGGCAAAGGGATCGATTTAATTCGTCATACCATCAGCACCAACTACACGGGGCGAATT
ATTTTCATTACCGCCGACAATCATATGGATACCATCAGCGATGCCTTACGCATGGGCGTATTCGATTATCTGATTAAGCC
GGTACATTATCAGCGCCTGCAGCATACCCTGGAGCGGTTCACCCGTTACCGCAGTTCGTTACGTTCCAGTGAGCAGGCAA
ACCAGACGCACGTTGATGCCTTATTTAATATCCAGGCCAAAGAACAAACCGCAGAACCGAATAGCGGACTGCGGGGAATT
GATGAAAACACCTTCAACCGGGTACGACAAATATTCGCCGACCCGCAGGCAGTACATACAGCAGACACTCTGGCGCAAAT
TCTGGGCAGCAGTAAAACCACTGCCCGACGCTATCTGGAACAAGGCGTGAAAAATAACTTTCTCGAAGCGGAGATCAGCT
ACGGGAAAGTGGGCAGGCCGGAGAGACTCTATCGCGGCAAAGTATGA

Upstream 100 bases:

>100_bases
AGCTACGTCAAGCAGGCCAATGGCGACATTATCGTCGATAACAATAATTCTTTTGGCACCATTTTTACCCTCTATATCCC
TTTTACCAGAGAAGAATAAC

Downstream 100 bases:

>100_bases
GATTATGGCGCCAACATGTAATTACGGCACATAGGCAAACACCTCAGCCACCCACTGACGAAAACCGTCCACATCCAGCG
CCAGCGCCACCTGCGCGTTG

Product: transcriptional regulator CitB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MDSITTLIVEDEPMLAEILVDTIKLFPQFSIVGIADKLESAKKQIRLYQPQLILLDNFLPDGKGIDLIRHTISTNYTGRI
IFITADNHMDTISDALRMGVFDYLIKPVHYQRLQHTLERFTRYRSSLRSSEQANQTHVDALFNIQAKEQTAEPNSGLRGI
DENTFNRVRQIFADPQAVHTADTLAQILGSSKTTARRYLEQGVKNNFLEAEISYGKVGRPERLYRGKV

Sequences:

>Translated_228_residues
MDSITTLIVEDEPMLAEILVDTIKLFPQFSIVGIADKLESAKKQIRLYQPQLILLDNFLPDGKGIDLIRHTISTNYTGRI
IFITADNHMDTISDALRMGVFDYLIKPVHYQRLQHTLERFTRYRSSLRSSEQANQTHVDALFNIQAKEQTAEPNSGLRGI
DENTFNRVRQIFADPQAVHTADTLAQILGSSKTTARRYLEQGVKNNFLEAEISYGKVGRPERLYRGKV
>Mature_228_residues
MDSITTLIVEDEPMLAEILVDTIKLFPQFSIVGIADKLESAKKQIRLYQPQLILLDNFLPDGKGIDLIRHTISTNYTGRI
IFITADNHMDTISDALRMGVFDYLIKPVHYQRLQHTLERFTRYRSSLRSSEQANQTHVDALFNIQAKEQTAEPNSGLRGI
DENTFNRVRQIFADPQAVHTADTLAQILGSSKTTARRYLEQGVKNNFLEAEISYGKVGRPERLYRGKV

Specific function: Member of the two-component regulatory system CitA/CitB essential for expression of citrate-specific fermentation genes. Phosphorylated CitB binds to two sites in the citS-citC intergenic region where it probably activates transcription of both genes [H]

COG id: COG4565

COG function: function code KT; Response regulator of citrate/malate metabolism

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786838, Length=222, Percent_Identity=47.2972972972973, Blast_Score=204, Evalue=3e-54,
Organism=Escherichia coli, GI1790566, Length=225, Percent_Identity=29.7777777777778, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 25996; Mature: 25996

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSITTLIVEDEPMLAEILVDTIKLFPQFSIVGIADKLESAKKQIRLYQPQLILLDNFLP
CCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEECCCC
DGKGIDLIRHTISTNYTGRIIFITADNHMDTISDALRMGVFDYLIKPVHYQRLQHTLERF
CCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRYRSSLRSSEQANQTHVDALFNIQAKEQTAEPNSGLRGIDENTFNRVRQIFADPQAVHT
HHHHHHHHHHHHCCHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHH
ADTLAQILGSSKTTARRYLEQGVKNNFLEAEISYGKVGRPERLYRGKV
HHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHCCCC
>Mature Secondary Structure
MDSITTLIVEDEPMLAEILVDTIKLFPQFSIVGIADKLESAKKQIRLYQPQLILLDNFLP
CCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEECCCC
DGKGIDLIRHTISTNYTGRIIFITADNHMDTISDALRMGVFDYLIKPVHYQRLQHTLERF
CCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRYRSSLRSSEQANQTHVDALFNIQAKEQTAEPNSGLRGIDENTFNRVRQIFADPQAVHT
HHHHHHHHHHHHCCHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHH
ADTLAQILGSSKTTARRYLEQGVKNNFLEAEISYGKVGRPERLYRGKV
HHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8748036; 9223636; 10447894 [H]