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The map label for this gene is lepA2

Identifier: 28379530

GI number: 28379530

Start: 2784795

End: 2786582

Strand: Direct

Name: lepA2

Synonym: lp_3120

Alternate gene names: 28379530

Gene position: 2784795-2786582 (Clockwise)

Preceding gene: 28379529

Following gene: 28379534

Centisome position: 84.18

GC content: 48.83

Gene sequence:

>1788_bases
ATGAAACAATCCCATATCCGTAACTTCGCCATCATCGCCCACATTGATCACGGTAAATCAACGTTGGCGGATCAAATCAT
GTCGCTGACCCAGACCGTCAGTGCGCGTGAACAGCACGCCCAATTATTAGACGATATGACCGTTGAACAGGCTCATGGCG
TGACTGTCAAAGCACGTACAGTTCGGAATTACTATCAGGCCGATGATGGTCAAGAATATGAATATAACTTAATTGACACC
CCTGGTCACGTTGATTTCAATTACGAAGTGGCTAAAAGCTTGGCTGCTACCGAGGGTGCCATCCTATTAGTAGACGCCAC
TCAGGGCGTTCAAGCGCAGACCATTGCCAATTACCGAATTGCCAAACAACGTCAATTAACCTTGATACCAGTTCTCAATA
AAGTTGATCTGCCCTCTGCGGATATTGACGCTGCGTTGGCCCAATTGAACGACCTCGACTCAGCTTTCACACCCGAGCAA
GTCTTGCAAATCTCCGCTAAAACCGGGCAAGGCGTGCCGGCCGTCTTGGAAGCCATCAAGCAGCGGTTGCCAGCTCCCCA
AGGCGACTTACACCAGCCGCTGAAAGCTTTAGTCTTTGATTCGTTGTATGACCCATATCAAGGTGTCATTGCCTATGTCC
GACTGATTGATGGTCAGCTCAAATCACAACAAGCACTGTGCTTAATGCAAGGACAACAGGATTTTAACGGCAAGGCCATT
GGTGTCTTCGCGCCTCAAATGCACCCCCAAGAATCGCTAAGCGCTGGTGACGTCGGTTACGTGGTCACTGGTATTAAAGA
TCCACGAAAAGTCCGTGTTGGTGATACGCTGACGTCCGTCGCCACCCCCACGCAGCGGCCGCTAGCCGGCTACCAACCAG
CCAAATCAATGGTCTTCGCGGGCTTATATCCTAAGAATAATGACTATCCGGCACTTAAGGAAGCCGTTCAAAAATTGAAC
TTGAACGACCCGTCATTTACCTATGTTGAAGAACGATCCGAGGCGCTTGGCGTTGGCTTCCGTTGCGGATTTCTAGGAAC
CTTTCACCTCCAAATCATTCGTGAACGACTCCACGATGAGTACGGGGTCGACGTGCTAACAACGGCTCCCAATGTGACTT
ACCACGTTACACTCACCAATGGTCAGCAAGTGATCGTCAATAATCCCGTTCAGTTTCCGGCATTCAGTCTCATTAAAGAA
GTGACTGAACCCTTTATGAAAGCCGAGATCACGATGCCTGCTGACAATCTAAATGCCGTATTAAAGCTTGCGGAACAGCA
TAAAGGGACCTTGATCGACTTGGCAAACAGCGGGGACTTGATTGTTGCAAGTCTCAAGATACCCTTGTCAGAAATCGCCT
ACCACTTTTTCTCAGAACTAAAATCAGTCTCACATGGCTTTGCGAGTTTAAGCACCGCCTTTATGGCTAATGAAGTCAGT
GATCTAGTCAAAGTTGAAGTTGATATCAACTATGCGCCCGTAGATGCGTTGACCTTCATCGTCCATCGTGAAGACGCCCC
CAACATGACGCAGCAATTGGTCGCCAATCTGAAGACGACGGTTCCACGGCAACTATATCCCACACCCGTCCAAGCACGTG
TTGAAGGTAAGGTTATCGCCCGCGTCGATGTCCCACCATTGCGTAAAAATGCAGCGGTGAACGGCGAACAACACAGTACT
TCCAAAAAAGCGGCCCTACTACGACGGCAAAGCGCTAATAAACGCCGCGCGTCCAAAAATACGATTAAGCTGCCACAAAG
TGTTTTTAATGCAATTTTAAGCCTTTAA

Upstream 100 bases:

>100_bases
TATCCGTTGATTATCCCGACTTCGAAATTCGACTCATACCGGAGGCCCAACAACAAATTCGTTACTACTTGACCCAATAT
TTGAAGGAGTTTTCGCACAA

Downstream 100 bases:

>100_bases
TCAAGTTAGACCATCCGTTGATTTCTGGGTTTCAATCAATCGGGTGAAACTAGCCCTTGCAATGCAGCCAATCAGACGTT
GGTTTTCACGTTTAAACAAC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4 2; Ribosomal back-translocase LepA 2

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MKQSHIRNFAIIAHIDHGKSTLADQIMSLTQTVSAREQHAQLLDDMTVEQAHGVTVKARTVRNYYQADDGQEYEYNLIDT
PGHVDFNYEVAKSLAATEGAILLVDATQGVQAQTIANYRIAKQRQLTLIPVLNKVDLPSADIDAALAQLNDLDSAFTPEQ
VLQISAKTGQGVPAVLEAIKQRLPAPQGDLHQPLKALVFDSLYDPYQGVIAYVRLIDGQLKSQQALCLMQGQQDFNGKAI
GVFAPQMHPQESLSAGDVGYVVTGIKDPRKVRVGDTLTSVATPTQRPLAGYQPAKSMVFAGLYPKNNDYPALKEAVQKLN
LNDPSFTYVEERSEALGVGFRCGFLGTFHLQIIRERLHDEYGVDVLTTAPNVTYHVTLTNGQQVIVNNPVQFPAFSLIKE
VTEPFMKAEITMPADNLNAVLKLAEQHKGTLIDLANSGDLIVASLKIPLSEIAYHFFSELKSVSHGFASLSTAFMANEVS
DLVKVEVDINYAPVDALTFIVHREDAPNMTQQLVANLKTTVPRQLYPTPVQARVEGKVIARVDVPPLRKNAAVNGEQHST
SKKAALLRRQSANKRRASKNTIKLPQSVFNAILSL

Sequences:

>Translated_595_residues
MKQSHIRNFAIIAHIDHGKSTLADQIMSLTQTVSAREQHAQLLDDMTVEQAHGVTVKARTVRNYYQADDGQEYEYNLIDT
PGHVDFNYEVAKSLAATEGAILLVDATQGVQAQTIANYRIAKQRQLTLIPVLNKVDLPSADIDAALAQLNDLDSAFTPEQ
VLQISAKTGQGVPAVLEAIKQRLPAPQGDLHQPLKALVFDSLYDPYQGVIAYVRLIDGQLKSQQALCLMQGQQDFNGKAI
GVFAPQMHPQESLSAGDVGYVVTGIKDPRKVRVGDTLTSVATPTQRPLAGYQPAKSMVFAGLYPKNNDYPALKEAVQKLN
LNDPSFTYVEERSEALGVGFRCGFLGTFHLQIIRERLHDEYGVDVLTTAPNVTYHVTLTNGQQVIVNNPVQFPAFSLIKE
VTEPFMKAEITMPADNLNAVLKLAEQHKGTLIDLANSGDLIVASLKIPLSEIAYHFFSELKSVSHGFASLSTAFMANEVS
DLVKVEVDINYAPVDALTFIVHREDAPNMTQQLVANLKTTVPRQLYPTPVQARVEGKVIARVDVPPLRKNAAVNGEQHST
SKKAALLRRQSANKRRASKNTIKLPQSVFNAILSL
>Mature_595_residues
MKQSHIRNFAIIAHIDHGKSTLADQIMSLTQTVSAREQHAQLLDDMTVEQAHGVTVKARTVRNYYQADDGQEYEYNLIDT
PGHVDFNYEVAKSLAATEGAILLVDATQGVQAQTIANYRIAKQRQLTLIPVLNKVDLPSADIDAALAQLNDLDSAFTPEQ
VLQISAKTGQGVPAVLEAIKQRLPAPQGDLHQPLKALVFDSLYDPYQGVIAYVRLIDGQLKSQQALCLMQGQQDFNGKAI
GVFAPQMHPQESLSAGDVGYVVTGIKDPRKVRVGDTLTSVATPTQRPLAGYQPAKSMVFAGLYPKNNDYPALKEAVQKLN
LNDPSFTYVEERSEALGVGFRCGFLGTFHLQIIRERLHDEYGVDVLTTAPNVTYHVTLTNGQQVIVNNPVQFPAFSLIKE
VTEPFMKAEITMPADNLNAVLKLAEQHKGTLIDLANSGDLIVASLKIPLSEIAYHFFSELKSVSHGFASLSTAFMANEVS
DLVKVEVDINYAPVDALTFIVHREDAPNMTQQLVANLKTTVPRQLYPTPVQARVEGKVIARVDVPPLRKNAAVNGEQHST
SKKAALLRRQSANKRRASKNTIKLPQSVFNAILSL

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=603, Percent_Identity=38.1426202321725, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI94966754, Length=237, Percent_Identity=32.4894514767932, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI4503483, Length=147, Percent_Identity=36.734693877551, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI25306283, Length=186, Percent_Identity=34.4086021505376, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI18390331, Length=199, Percent_Identity=29.1457286432161, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI19923640, Length=186, Percent_Identity=34.4086021505376, Blast_Score=93, Evalue=9e-19,
Organism=Homo sapiens, GI25306287, Length=186, Percent_Identity=34.4086021505376, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=37.8378378378378, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=37.8378378378378, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=37.8378378378378, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI217272894, Length=178, Percent_Identity=30.3370786516854, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI217272892, Length=178, Percent_Identity=30.3370786516854, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI34147630, Length=291, Percent_Identity=26.8041237113402, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1788922, Length=591, Percent_Identity=42.6395939086294, Blast_Score=475, Evalue=1e-135,
Organism=Escherichia coli, GI48994988, Length=497, Percent_Identity=25.5533199195171, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789738, Length=148, Percent_Identity=32.4324324324324, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1790835, Length=138, Percent_Identity=30.4347826086957, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1789559, Length=227, Percent_Identity=31.7180616740088, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1789737, Length=132, Percent_Identity=32.5757575757576, Blast_Score=63, Evalue=6e-11,
Organism=Escherichia coli, GI1790412, Length=132, Percent_Identity=32.5757575757576, Blast_Score=63, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=34.1503267973856, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=36.0902255639098, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=183, Percent_Identity=32.7868852459016, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=36.0902255639098, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17506493, Length=159, Percent_Identity=31.4465408805031, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17556745, Length=166, Percent_Identity=30.7228915662651, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17533571, Length=156, Percent_Identity=32.0512820512821, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17556456, Length=139, Percent_Identity=33.0935251798561, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=599, Percent_Identity=36.7278797996661, Blast_Score=411, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6323098, Length=195, Percent_Identity=31.2820512820513, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6324707, Length=147, Percent_Identity=38.0952380952381, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320593, Length=147, Percent_Identity=38.0952380952381, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322359, Length=156, Percent_Identity=29.4871794871795, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324166, Length=143, Percent_Identity=36.3636363636364, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=239, Percent_Identity=25.9414225941423, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI78706572, Length=604, Percent_Identity=36.7549668874172, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI28574573, Length=174, Percent_Identity=37.9310344827586, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585711, Length=151, Percent_Identity=35.7615894039735, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585713, Length=151, Percent_Identity=35.7615894039735, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585709, Length=151, Percent_Identity=35.7615894039735, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI221458488, Length=191, Percent_Identity=33.5078534031414, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24582462, Length=150, Percent_Identity=32.6666666666667, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=32.3308270676692, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28572034, Length=268, Percent_Identity=28.3582089552239, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281363316, Length=290, Percent_Identity=26.2068965517241, Blast_Score=67, Evalue=5e-11,
Organism=Drosophila melanogaster, GI17864358, Length=290, Percent_Identity=26.2068965517241, Blast_Score=67, Evalue=5e-11,
Organism=Drosophila melanogaster, GI19921738, Length=220, Percent_Identity=28.6363636363636, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA2_LACPL (Q88T65)

Other databases:

- EMBL:   AL935261
- RefSeq:   NP_786422.1
- ProteinModelPortal:   Q88T65
- SMR:   Q88T65
- GeneID:   1063821
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_3120
- NMPDR:   fig|220668.1.peg.2556
- HOGENOM:   HBG286375
- OMA:   FMANEVS
- ProtClustDB:   PRK05433
- BioCyc:   LPLA220668:LP_3120-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 65272; Mature: 65272

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSHIRNFAIIAHIDHGKSTLADQIMSLTQTVSAREQHAQLLDDMTVEQAHGVTVKART
CCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHH
VRNYYQADDGQEYEYNLIDTPGHVDFNYEVAKSLAATEGAILLVDATQGVQAQTIANYRI
HHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
AKQRQLTLIPVLNKVDLPSADIDAALAQLNDLDSAFTPEQVLQISAKTGQGVPAVLEAIK
HCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHEEECCCCCCCHHHHHHHH
QRLPAPQGDLHQPLKALVFDSLYDPYQGVIAYVRLIDGQLKSQQALCLMQGQQDFNGKAI
HHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHCCCHHHHEECCCCCCCCCEE
GVFAPQMHPQESLSAGDVGYVVTGIKDPRKVRVGDTLTSVATPTQRPLAGYQPAKSMVFA
EEECCCCCCHHCCCCCCCCEEEECCCCCCEEEECCHHHHHCCCCCCCCCCCCCHHHEEEE
GLYPKNNDYPALKEAVQKLNLNDPSFTYVEERSEALGVGFRCGFLGTFHLQIIRERLHDE
EECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHH
YGVDVLTTAPNVTYHVTLTNGQQVIVNNPVQFPAFSLIKEVTEPFMKAEITMPADNLNAV
HCCEEEEECCCEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHEEEECCCHHHHHH
LKLAEQHKGTLIDLANSGDLIVASLKIPLSEIAYHFFSELKSVSHGFASLSTAFMANEVS
HHHHHHCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLVKVEVDINYAPVDALTFIVHREDAPNMTQQLVANLKTTVPRQLYPTPVQARVEGKVIA
CEEEEEEECCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCEEEE
RVDVPPLRKNAAVNGEQHSTSKKAALLRRQSANKRRASKNTIKLPQSVFNAILSL
EECCCCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHCC
>Mature Secondary Structure
MKQSHIRNFAIIAHIDHGKSTLADQIMSLTQTVSAREQHAQLLDDMTVEQAHGVTVKART
CCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHH
VRNYYQADDGQEYEYNLIDTPGHVDFNYEVAKSLAATEGAILLVDATQGVQAQTIANYRI
HHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
AKQRQLTLIPVLNKVDLPSADIDAALAQLNDLDSAFTPEQVLQISAKTGQGVPAVLEAIK
HCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHEEECCCCCCCHHHHHHHH
QRLPAPQGDLHQPLKALVFDSLYDPYQGVIAYVRLIDGQLKSQQALCLMQGQQDFNGKAI
HHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHCCCHHHHEECCCCCCCCCEE
GVFAPQMHPQESLSAGDVGYVVTGIKDPRKVRVGDTLTSVATPTQRPLAGYQPAKSMVFA
EEECCCCCCHHCCCCCCCCEEEECCCCCCEEEECCHHHHHCCCCCCCCCCCCCHHHEEEE
GLYPKNNDYPALKEAVQKLNLNDPSFTYVEERSEALGVGFRCGFLGTFHLQIIRERLHDE
EECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHH
YGVDVLTTAPNVTYHVTLTNGQQVIVNNPVQFPAFSLIKEVTEPFMKAEITMPADNLNAV
HCCEEEEECCCEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHEEEECCCHHHHHH
LKLAEQHKGTLIDLANSGDLIVASLKIPLSEIAYHFFSELKSVSHGFASLSTAFMANEVS
HHHHHHCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLVKVEVDINYAPVDALTFIVHREDAPNMTQQLVANLKTTVPRQLYPTPVQARVEGKVIA
CEEEEEEECCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCEEEE
RVDVPPLRKNAAVNGEQHSTSKKAALLRRQSANKRRASKNTIKLPQSVFNAILSL
EECCCCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12566566