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The map label for this gene is pflB1 [H]

Identifier: 28379125

GI number: 28379125

Start: 2315865

End: 2318309

Strand: Direct

Name: pflB1 [H]

Synonym: lp_2598

Alternate gene names: 28379125

Gene position: 2315865-2318309 (Clockwise)

Preceding gene: 28379124

Following gene: 28379126

Centisome position: 70.0

GC content: 42.21

Gene sequence:

>2445_bases
ATGATCATGTCTGAAACTTTAACTAAAACAACGACAACTATTAACCACTTCGGTAAATTGACGCCAATGATGGATCGCTT
ACGCGATAGCATCATTGATGCAAAACCTTATGTCGATCCAGAACGGGCGATTCTCACAACCGAAACTTATCGACAACACC
AAGACGAACAAGTCGATATATTACGGGCTAAAATGCTTGAACACGTTCTTGATAAAATGAGTATCTTCATTGAAGATGAT
ACTTTAATTGTTGGTAACCAAGCACGCCAAAATCGTTGGGCACCAGTATTCCCTGAGTATTCTATGAATTGGGTCATTGA
TGAATTAGATACATTTGAGAAGCGTCCTGGTGACGTTTTCTATATTACGGAGAAATCCAAGGAAGAACTTCGTGCGATTG
CGCCTTTCTGGAAACATAATACCTTGGAAGACCGCGGCTACGCTAGTTTTCCAGAAGCAAGTCGTATTTTTTATGATTTA
GGTATTATTGGAGCCGATGGTAATATCACTTCTGGTGATGGTCACATTGCGGTCGACTATAAAAACGTTGTTAATAAGGG
ACTTAAATGGTATGAAGACCGCATTAAGACAGCACTTGCTAATCTTGACCTTACTGATTTTAACCAGCAAAAACAATACT
ATTTCTATAAAGCGGGCCTAATTGTAATTGATGCCATTCACAATTTTGCTAAACGTTACGCCCAATTAGCGTCCAAGCAA
GCTCAAAACACGACATCCGCAACTCGCAAAGCACAACTTGAAAAAATCGCCCAAATTCTAAACAAGGTTCCTTACGAACC
TGCAAATTCATTTTATGAAGCGATTCAAGCTGTCTGGTTAGTTCATCTGACCTTACAAATCGAATCCAACGGTCATTCTG
TCTCATATGGTCGTCTAGATCAGTACCTAGCTCCATTCTATGAGCACGATTTAAAAACTGGTGCTATTGACGCCAACGGT
GCAACCGAATTACTCACAAACTTATGTCTTAAGACGTTAACGATTAATAAAGTACGCTCATGGCAACATACTGAATTTTC
TGCAGGGAGTCCCCTCTACCAAAACATTACGATTGGTGGTCAAACACCAGATGGTAAAGATGCCGTTAATCCGACGTCCT
ATCTGATTTTACGAGCAATTGCGCAAGCACATTTACCACAACCCAACTTAACGGTCCGTTATCACCATGGCTTAAGCGAT
AAGTTTATGCGTGAATGTGTCGAAGTTATTAAACAAGGCTTAGGTATGCCTGCGTTTAATAACGACGAAATTATTATTCC
GTCGTTTATTCGTCGTGGCGTCAAGAAAGAAGACGCCTATAATTACAGTGCCATCGGTTGTGTCGAAACAGCGATCCCTG
GAAAATGGGGCTATCGTTGCACCGGGATGAGCTTCATTAACTTCCCACGCGTTCTCTTACTCATTATGAATGGTGGCATT
GATCCTGAATCTGGCAAACGGTTATTACCCGATTATGGTAAGTTCACTGATATGACTTCTTTTGATCAACTTATGACTGC
TTGGGACAAAGCGCTCCGTGAAATGACACGACAAAGTGTGATTATCGAAAATAGTTGTGATTTGGCTTTGGAACAAAATT
ATCCTGATATTCTCTGCTCCGTTTTAACCGACGATTGTATCGGTCGTGGTAAGACCATTAAAGAAGGTGGCGCGGTATAC
GACTTTATCAGTGGATTACAAGTTGGTATTGCTAACCTAGCGGACTCCCTAGCTGCAATCAAGAAACTTGTCTTTGAAGA
AAAGAAGTTGACAACAACCCAACTTTGGCACGCACTTACCACTGATTTTGCGGATGAAGATGGTGAAAAGATTCGGCAGA
TGCTCATTAATGATGCCCCAAAGTATGGTAACGATGATGATTATGTTGATGATTTGATTGTTGAAGCTTATAAACCATAT
ATTGATGAAATTGCCAAGTACAAAAACACGCGCTACGGTCGCGGCCCTATTGGTGGCTTGCGCTACGCAGGAACCTCTTC
TATTTCGGCCAACGTTGGTCAAGGGCACAGCACTTTGGCTACACCAGATGGTCGGCACGCTCGGACACCATTAGCCGAAG
GTTGCTCACCAGAACATGCAATGGATACTGATGGCCCAACTGCTGTGTTCAAATCAGTTTCCAAATTATCCACTAAGGAC
ATCACTGGTGGCGTATTACTGAACCAAAAGATGTCACCACAAATTCTACGGAGTGATGAAAGCTGCATGAAATTGGTTGC
ACTACTACGGACCTTCTTCAATCGACTTCATGGTTACCATGTCCAATACAACATTGTTTCACGGGATACCTTGATTGATG
CACAGAACCATCCTGACAAGCACCGTGACTTGATTGTTCGGGTTGCTGGATATTCCGCCTTCTTCGTGGGCCTATCCAAA
GAAACCCAAGATGATATTATCGAACGGACGGAGCAGTCTCTATAA

Upstream 100 bases:

>100_bases
ATTTTATAATTATTCATTATTTTGATGATATTTCTAGTCAAAAGATGCTATGATTATTACGTAAGAAAGTCATTTCAGTT
TCTAACGATAGAAAGGAAGA

Downstream 100 bases:

>100_bases
TCTATATAGTTTGCTAGTCCAAGTAATTTAAAAAGTATTGTCTTAAGGCTCCTACCAATCCACTCTCAGCTGGTTTCGCT
TGGAGCAACAATTAAGAAGT

Product: formate C-acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 3 [H]

Number of amino acids: Translated: 814; Mature: 814

Protein sequence:

>814_residues
MIMSETLTKTTTTINHFGKLTPMMDRLRDSIIDAKPYVDPERAILTTETYRQHQDEQVDILRAKMLEHVLDKMSIFIEDD
TLIVGNQARQNRWAPVFPEYSMNWVIDELDTFEKRPGDVFYITEKSKEELRAIAPFWKHNTLEDRGYASFPEASRIFYDL
GIIGADGNITSGDGHIAVDYKNVVNKGLKWYEDRIKTALANLDLTDFNQQKQYYFYKAGLIVIDAIHNFAKRYAQLASKQ
AQNTTSATRKAQLEKIAQILNKVPYEPANSFYEAIQAVWLVHLTLQIESNGHSVSYGRLDQYLAPFYEHDLKTGAIDANG
ATELLTNLCLKTLTINKVRSWQHTEFSAGSPLYQNITIGGQTPDGKDAVNPTSYLILRAIAQAHLPQPNLTVRYHHGLSD
KFMRECVEVIKQGLGMPAFNNDEIIIPSFIRRGVKKEDAYNYSAIGCVETAIPGKWGYRCTGMSFINFPRVLLLIMNGGI
DPESGKRLLPDYGKFTDMTSFDQLMTAWDKALREMTRQSVIIENSCDLALEQNYPDILCSVLTDDCIGRGKTIKEGGAVY
DFISGLQVGIANLADSLAAIKKLVFEEKKLTTTQLWHALTTDFADEDGEKIRQMLINDAPKYGNDDDYVDDLIVEAYKPY
IDEIAKYKNTRYGRGPIGGLRYAGTSSISANVGQGHSTLATPDGRHARTPLAEGCSPEHAMDTDGPTAVFKSVSKLSTKD
ITGGVLLNQKMSPQILRSDESCMKLVALLRTFFNRLHGYHVQYNIVSRDTLIDAQNHPDKHRDLIVRVAGYSAFFVGLSK
ETQDDIIERTEQSL

Sequences:

>Translated_814_residues
MIMSETLTKTTTTINHFGKLTPMMDRLRDSIIDAKPYVDPERAILTTETYRQHQDEQVDILRAKMLEHVLDKMSIFIEDD
TLIVGNQARQNRWAPVFPEYSMNWVIDELDTFEKRPGDVFYITEKSKEELRAIAPFWKHNTLEDRGYASFPEASRIFYDL
GIIGADGNITSGDGHIAVDYKNVVNKGLKWYEDRIKTALANLDLTDFNQQKQYYFYKAGLIVIDAIHNFAKRYAQLASKQ
AQNTTSATRKAQLEKIAQILNKVPYEPANSFYEAIQAVWLVHLTLQIESNGHSVSYGRLDQYLAPFYEHDLKTGAIDANG
ATELLTNLCLKTLTINKVRSWQHTEFSAGSPLYQNITIGGQTPDGKDAVNPTSYLILRAIAQAHLPQPNLTVRYHHGLSD
KFMRECVEVIKQGLGMPAFNNDEIIIPSFIRRGVKKEDAYNYSAIGCVETAIPGKWGYRCTGMSFINFPRVLLLIMNGGI
DPESGKRLLPDYGKFTDMTSFDQLMTAWDKALREMTRQSVIIENSCDLALEQNYPDILCSVLTDDCIGRGKTIKEGGAVY
DFISGLQVGIANLADSLAAIKKLVFEEKKLTTTQLWHALTTDFADEDGEKIRQMLINDAPKYGNDDDYVDDLIVEAYKPY
IDEIAKYKNTRYGRGPIGGLRYAGTSSISANVGQGHSTLATPDGRHARTPLAEGCSPEHAMDTDGPTAVFKSVSKLSTKD
ITGGVLLNQKMSPQILRSDESCMKLVALLRTFFNRLHGYHVQYNIVSRDTLIDAQNHPDKHRDLIVRVAGYSAFFVGLSK
ETQDDIIERTEQSL
>Mature_814_residues
MIMSETLTKTTTTINHFGKLTPMMDRLRDSIIDAKPYVDPERAILTTETYRQHQDEQVDILRAKMLEHVLDKMSIFIEDD
TLIVGNQARQNRWAPVFPEYSMNWVIDELDTFEKRPGDVFYITEKSKEELRAIAPFWKHNTLEDRGYASFPEASRIFYDL
GIIGADGNITSGDGHIAVDYKNVVNKGLKWYEDRIKTALANLDLTDFNQQKQYYFYKAGLIVIDAIHNFAKRYAQLASKQ
AQNTTSATRKAQLEKIAQILNKVPYEPANSFYEAIQAVWLVHLTLQIESNGHSVSYGRLDQYLAPFYEHDLKTGAIDANG
ATELLTNLCLKTLTINKVRSWQHTEFSAGSPLYQNITIGGQTPDGKDAVNPTSYLILRAIAQAHLPQPNLTVRYHHGLSD
KFMRECVEVIKQGLGMPAFNNDEIIIPSFIRRGVKKEDAYNYSAIGCVETAIPGKWGYRCTGMSFINFPRVLLLIMNGGI
DPESGKRLLPDYGKFTDMTSFDQLMTAWDKALREMTRQSVIIENSCDLALEQNYPDILCSVLTDDCIGRGKTIKEGGAVY
DFISGLQVGIANLADSLAAIKKLVFEEKKLTTTQLWHALTTDFADEDGEKIRQMLINDAPKYGNDDDYVDDLIVEAYKPY
IDEIAKYKNTRYGRGPIGGLRYAGTSSISANVGQGHSTLATPDGRHARTPLAEGCSPEHAMDTDGPTAVFKSVSKLSTKD
ITGGVLLNQKMSPQILRSDESCMKLVALLRTFFNRLHGYHVQYNIVSRDTLIDAQNHPDKHRDLIVRVAGYSAFFVGLSK
ETQDDIIERTEQSL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787044, Length=806, Percent_Identity=50.8684863523573, Blast_Score=814, Evalue=0.0,
Organism=Escherichia coli, GI1790388, Length=798, Percent_Identity=37.34335839599, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI1787131, Length=580, Percent_Identity=27.4137931034483, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI48994926, Length=584, Percent_Identity=26.541095890411, Blast_Score=184, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 91616; Mature: 91616

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMSETLTKTTTTINHFGKLTPMMDRLRDSIIDAKPYVDPERAILTTETYRQHQDEQVDI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHCCCHHHHH
LRAKMLEHVLDKMSIFIEDDTLIVGNQARQNRWAPVFPEYSMNWVIDELDTFEKRPGDVF
HHHHHHHHHHHHHEEEEECCEEEECCCHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEE
YITEKSKEELRAIAPFWKHNTLEDRGYASFPEASRIFYDLGIIGADGNITSGDGHIAVDY
EEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEEEEEECCCCEECCCCEEEEEH
KNVVNKGLKWYEDRIKTALANLDLTDFNQQKQYYFYKAGLIVIDAIHNFAKRYAQLASKQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
AQNTTSATRKAQLEKIAQILNKVPYEPANSFYEAIQAVWLVHLTLQIESNGHSVSYGRLD
HCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEEEEECCCCEECHHHHH
QYLAPFYEHDLKTGAIDANGATELLTNLCLKTLTINKVRSWQHTEFSAGSPLYQNITIGG
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
QTPDGKDAVNPTSYLILRAIAQAHLPQPNLTVRYHHGLSDKFMRECVEVIKQGLGMPAFN
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCC
NDEIIIPSFIRRGVKKEDAYNYSAIGCVETAIPGKWGYRCTGMSFINFPRVLLLIMNGGI
CCCEECHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHCCCC
DPESGKRLLPDYGKFTDMTSFDQLMTAWDKALREMTRQSVIIENSCDLALEQNYPDILCS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEECCCCHHHHHH
VLTDDCIGRGKTIKEGGAVYDFISGLQVGIANLADSLAAIKKLVFEEKKLTTTQLWHALT
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDFADEDGEKIRQMLINDAPKYGNDDDYVDDLIVEAYKPYIDEIAKYKNTRYGRGPIGGL
HHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
RYAGTSSISANVGQGHSTLATPDGRHARTPLAEGCSPEHAMDTDGPTAVFKSVSKLSTKD
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHCC
ITGGVLLNQKMSPQILRSDESCMKLVALLRTFFNRLHGYHVQYNIVSRDTLIDAQNHPDK
CCCCEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCEEECCCCCCCH
HRDLIVRVAGYSAFFVGLSKETQDDIIERTEQSL
HHEEEEEEECCEEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIMSETLTKTTTTINHFGKLTPMMDRLRDSIIDAKPYVDPERAILTTETYRQHQDEQVDI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHCCCHHHHH
LRAKMLEHVLDKMSIFIEDDTLIVGNQARQNRWAPVFPEYSMNWVIDELDTFEKRPGDVF
HHHHHHHHHHHHHEEEEECCEEEECCCHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEE
YITEKSKEELRAIAPFWKHNTLEDRGYASFPEASRIFYDLGIIGADGNITSGDGHIAVDY
EEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEEEEEECCCCEECCCCEEEEEH
KNVVNKGLKWYEDRIKTALANLDLTDFNQQKQYYFYKAGLIVIDAIHNFAKRYAQLASKQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
AQNTTSATRKAQLEKIAQILNKVPYEPANSFYEAIQAVWLVHLTLQIESNGHSVSYGRLD
HCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEEEEECCCCEECHHHHH
QYLAPFYEHDLKTGAIDANGATELLTNLCLKTLTINKVRSWQHTEFSAGSPLYQNITIGG
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
QTPDGKDAVNPTSYLILRAIAQAHLPQPNLTVRYHHGLSDKFMRECVEVIKQGLGMPAFN
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCC
NDEIIIPSFIRRGVKKEDAYNYSAIGCVETAIPGKWGYRCTGMSFINFPRVLLLIMNGGI
CCCEECHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHCCCC
DPESGKRLLPDYGKFTDMTSFDQLMTAWDKALREMTRQSVIIENSCDLALEQNYPDILCS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEECCCCHHHHHH
VLTDDCIGRGKTIKEGGAVYDFISGLQVGIANLADSLAAIKKLVFEEKKLTTTQLWHALT
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDFADEDGEKIRQMLINDAPKYGNDDDYVDDLIVEAYKPYIDEIAKYKNTRYGRGPIGGL
HHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
RYAGTSSISANVGQGHSTLATPDGRHARTPLAEGCSPEHAMDTDGPTAVFKSVSKLSTKD
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHCC
ITGGVLLNQKMSPQILRSDESCMKLVALLRTFFNRLHGYHVQYNIVSRDTLIDAQNHPDK
CCCCEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCEEECCCCCCCH
HRDLIVRVAGYSAFFVGLSKETQDDIIERTEQSL
HHEEEEEEECCEEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]