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The map label for this gene is bsh4 [H]

Identifier: 28379105

GI number: 28379105

Start: 2290119

End: 2291072

Strand: Direct

Name: bsh4 [H]

Synonym: lp_2572

Alternate gene names: 28379105

Gene position: 2290119-2291072 (Clockwise)

Preceding gene: 28379100

Following gene: 28379108

Centisome position: 69.22

GC content: 52.31

Gene sequence:

>954_bases
ATGTGTACCAGCTTAACTTATCTTGATACTGACAATCACCGCTACTTCGCCCGCACCATGGACTTTCCAACAACGACACC
TTGGCGGCCAATTTTTTTGCCGCGCCGTTATCCGTGGCCAACTGGGTTAGCGACGACGCGTATGACGCAGTATGCCATTC
TCGGTGGTGGTCGGCTACCTGACCACTTTAAGGCTTGTTTGATGGCTGACAGCATTAACGAAGCTGGTTTGGTGTGTGCT
GAACTGTACTTGCCCCACGCCGTTGAATACGCCACTCAATCACAAGTCAACCAAATTAATTTAACACCCCAAGCCTTCAT
CAACTGGGCTTTAGGTGAACACCAATCAGTCGCAGCCGTGATCGCCGATCTGCCAAGTGTTAACCTGGTCGGTGCGTCCT
GGGGTGATGACACTGGTGAAGTCTATCCCTTTCACTGGTATCTCAGTGATGCACACACCAGTGCCGTCATCGAACCCACT
GGTGGCCCACTGACGGTGCAACCGAATCCAGCCGGCGTCCTGACCAATACACCAGTCCTAAGCGACCATCAGCGCCGACT
AAATCGTTATTTAGCAGTATCTGGCAACCAGATTACAACTGCCACTCGTCAGGCTGCTCAGCACGTGATTCAGACTAAGC
AACCATTACCGAGCGGGCCGATTCCCACTGATCGTTTCATTCACATGGCACTTCGACGACTGGGAACACCGCAGCTAGCA
CCGCAACAAGTGCCGACCACTTTATTCCGCTGGCTACAAGAAGTAAGCTTGCCATACCACGCCGACCGTCGCCATCTCAT
CAGCCACAACTACACGCACTATCGTTGTTTGATCACGTTAGCGACTCGTACTTACCGCTTTATTCCACGCACGACTGGTC
ACGAACAACGACTGACACTAACACCTGAAATGGCAACAACCTGGCGAACACCGTACCTCTTTCCTGCCGATTGA

Upstream 100 bases:

>100_bases
AGATCATCAAAAAAGTAACCGTTACCATCGTTAATTAAACAGTCATTTAGCTGTCACCCATTTTGACGTATAATGAACCT
AATTAAAAGGAGGCCGCGCC

Downstream 100 bases:

>100_bases
AACTAAAAAATCACGATGTTGAGACTGAACTCACATCGTGATTTTTAATTAATGACGCTGCTTCAAAAAATGATCAAATT
CCTGATTGATTGCTGCTCGT

Product: choloylglycine hydrolase

Products: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate; glycine

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MCTSLTYLDTDNHRYFARTMDFPTTTPWRPIFLPRRYPWPTGLATTRMTQYAILGGGRLPDHFKACLMADSINEAGLVCA
ELYLPHAVEYATQSQVNQINLTPQAFINWALGEHQSVAAVIADLPSVNLVGASWGDDTGEVYPFHWYLSDAHTSAVIEPT
GGPLTVQPNPAGVLTNTPVLSDHQRRLNRYLAVSGNQITTATRQAAQHVIQTKQPLPSGPIPTDRFIHMALRRLGTPQLA
PQQVPTTLFRWLQEVSLPYHADRRHLISHNYTHYRCLITLATRTYRFIPRTTGHEQRLTLTPEMATTWRTPYLFPAD

Sequences:

>Translated_317_residues
MCTSLTYLDTDNHRYFARTMDFPTTTPWRPIFLPRRYPWPTGLATTRMTQYAILGGGRLPDHFKACLMADSINEAGLVCA
ELYLPHAVEYATQSQVNQINLTPQAFINWALGEHQSVAAVIADLPSVNLVGASWGDDTGEVYPFHWYLSDAHTSAVIEPT
GGPLTVQPNPAGVLTNTPVLSDHQRRLNRYLAVSGNQITTATRQAAQHVIQTKQPLPSGPIPTDRFIHMALRRLGTPQLA
PQQVPTTLFRWLQEVSLPYHADRRHLISHNYTHYRCLITLATRTYRFIPRTTGHEQRLTLTPEMATTWRTPYLFPAD
>Mature_317_residues
MCTSLTYLDTDNHRYFARTMDFPTTTPWRPIFLPRRYPWPTGLATTRMTQYAILGGGRLPDHFKACLMADSINEAGLVCA
ELYLPHAVEYATQSQVNQINLTPQAFINWALGEHQSVAAVIADLPSVNLVGASWGDDTGEVYPFHWYLSDAHTSAVIEPT
GGPLTVQPNPAGVLTNTPVLSDHQRRLNRYLAVSGNQITTATRQAAQHVIQTKQPLPSGPIPTDRFIHMALRRLGTPQLA
PQQVPTTLFRWLQEVSLPYHADRRHLISHNYTHYRCLITLATRTYRFIPRTTGHEQRLTLTPEMATTWRTPYLFPAD

Specific function: Unknown

COG id: COG3049

COG function: function code M; Penicillin V acylase and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C59 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003199 [H]

Pfam domain/function: PF02275 CBAH [H]

EC number: 3.5.1.24

Molecular weight: Translated: 35767; Mature: 35767

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTSLTYLDTDNHRYFARTMDFPTTTPWRPIFLPRRYPWPTGLATTRMTQYAILGGGRLP
CCCEEEEEECCCCEEEEEEECCCCCCCCCEEECCCCCCCCCCCHHHHHEEEEEECCCCCC
DHFKACLMADSINEAGLVCAELYLPHAVEYATQSQVNQINLTPQAFINWALGEHQSVAAV
HHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHCCEEEECCHHHHHHHHCCCCHHHHHH
IADLPSVNLVGASWGDDTGEVYPFHWYLSDAHTSAVIEPTGGPLTVQPNPAGVLTNTPVL
HHCCCCCEEEECCCCCCCCCEEEEEEEEECCCCEEEEECCCCEEEECCCCCCEEECCCCC
SDHQRRLNRYLAVSGNQITTATRQAAQHVIQTKQPLPSGPIPTDRFIHMALRRLGTPQLA
CHHHHHHHHEEEECCCEEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCC
PQQVPTTLFRWLQEVSLPYHADRRHLISHNYTHYRCLITLATRTYRFIPRTTGHEQRLTL
CHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEEEEEECHHEEECCCCCCCCCEEEE
TPEMATTWRTPYLFPAD
CCCHHHHCCCCEEECCC
>Mature Secondary Structure
MCTSLTYLDTDNHRYFARTMDFPTTTPWRPIFLPRRYPWPTGLATTRMTQYAILGGGRLP
CCCEEEEEECCCCEEEEEEECCCCCCCCCEEECCCCCCCCCCCHHHHHEEEEEECCCCCC
DHFKACLMADSINEAGLVCAELYLPHAVEYATQSQVNQINLTPQAFINWALGEHQSVAAV
HHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHCCEEEECCHHHHHHHHCCCCHHHHHH
IADLPSVNLVGASWGDDTGEVYPFHWYLSDAHTSAVIEPTGGPLTVQPNPAGVLTNTPVL
HHCCCCCEEEECCCCCCCCCEEEEEEEEECCCCEEEEECCCCEEEECCCCCCEEECCCCC
SDHQRRLNRYLAVSGNQITTATRQAAQHVIQTKQPLPSGPIPTDRFIHMALRRLGTPQLA
CHHHHHHHHEEEECCCEEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCC
PQQVPTTLFRWLQEVSLPYHADRRHLISHNYTHYRCLITLATRTYRFIPRTTGHEQRLTL
CHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEEEEEECHHEEECCCCCCCCCEEEE
TPEMATTWRTPYLFPAD
CCCHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine; H2O

Specific reaction: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine + H2O = 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]