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The map label for this gene is asnC

Identifier: 28378421

GI number: 28378421

Start: 1579487

End: 1580785

Strand: Direct

Name: asnC

Synonym: lp_1740

Alternate gene names: 28378421

Gene position: 1579487-1580785 (Clockwise)

Preceding gene: 28378420

Following gene: 28378422

Centisome position: 47.74

GC content: 43.42

Gene sequence:

>1299_bases
ATGGTGCAACAGATCAACATTATCGATGCTAAGGACCATGTCGACGAAAAAGTAAAGATTGGGGCTTGGCTCACTAATAA
ACGTTCAAGTGGTAAGATTGCCTTCCTACAATTACGTGATGGCTCCGCCTTTTTCCAAGGTGTCGTTGTTAAGAGCCAAG
TTAGCGAAGAAGTTTTTGAACTCGCTAAGGAAGTTAAGCAAGAAGCCAGTATGTGGATTACGGGTGTGATTCATGAAGAC
AGTCGTTCAAAGTTCGGTTATGAAATCGAAGTTGAAGACATTGTAGTCGTTGGTGAAAGTGAAGAATACCCAATTACGCC
TAAGGAACATGGGGTGGACTTCTTACTAGATCACCGGCATCTATGGTTACGTTCTAAGCGGCCATGGGCAATCATGAACA
TTCGTAATGAAGTCATTCGCGGAACGTATGAATTTTTTAATCAAGAAGGTTTCATTAAGATGGACTCACCAATTTTGACT
GGGAGTGCACCTGAAGGGACAACTGAATTGTTCCATACTGAATATTTTGATCGGGATGCCTACTTATCACAGAGTGGTCA
GTTATACGCCGAAGCCGGAGCTTTGGCCTACGGAAAGGTCTTTACTTTTGGCCCAACATTCCGGGCTGAAAAGTCCAAGA
CTCGGCGGCATTTAATTGAGTTCTGGATGATTGAACCAGAAATGGCCTTCATGCATCAAGAACAAAGTTTGGAAGTTCAA
GAACGTTATATTGCCTTTCTTGTTCAAGGTGTGATTGATCATTGTCAATACGCACTCGACATTTTAGGTCGTGATGTTGA
AACGTTGAAGAAGTACACCAAGTTACCTTATCCACGAATCAGTTACGATGAAGCCATTGAATTGTTAAAAGAAAATGACT
TTGACGTGGATTGGGGTGTTGATTTTGGTTCACCAGAAGAAACCTTCTTGGCTGATCACTTCGATCAACCCGTCTTCGTT
TTGAATTACCCGAAAGCCATCAAGCCATTTTATATGAAGCCGCATCCAACCCGGGACGACGTGGTGATTTGTGCGGATTT
ATTAGCCCCAGAAGGCTATGGTGAGATTATTGGCGGGTCAGAACGAGCAACTGATTACAATTACTTACTTGACCAGATCA
AACAAGCCGGCTTAAATCCAGACGACTATGCTTGGTACTTAGATTTACGTAAGTATGGTAGTGTTCCTCATGCTGGTTTT
GGTTTGGGACTCGAGCGATTCTTGACTTGGATTACGGCCGAAGATCACGTGCGGGAGACAATTCCGTTCCCACGGTTGTT
GAACCGGATTTACCCGTAA

Upstream 100 bases:

>100_bases
CAACTGATATCGATTCCTTGCGAGAGGCCCATCGACGGTTACAAAAATTTATGACAGAAAAAATTGCTGAACAAGCAAAG
TAATTGGAGGATGTAAGTAA

Downstream 100 bases:

>100_bases
ACTTTAATTGAAGGTTAGTTTTATTAAGAGCGTGGGACCTAGTCTCACGCTCTTAATAGTGAAAGGATGATAACGAGATG
GAGTCCTTAGCTGCCGCGTT

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase 2; AsnRS 2

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MVQQINIIDAKDHVDEKVKIGAWLTNKRSSGKIAFLQLRDGSAFFQGVVVKSQVSEEVFELAKEVKQEASMWITGVIHED
SRSKFGYEIEVEDIVVVGESEEYPITPKEHGVDFLLDHRHLWLRSKRPWAIMNIRNEVIRGTYEFFNQEGFIKMDSPILT
GSAPEGTTELFHTEYFDRDAYLSQSGQLYAEAGALAYGKVFTFGPTFRAEKSKTRRHLIEFWMIEPEMAFMHQEQSLEVQ
ERYIAFLVQGVIDHCQYALDILGRDVETLKKYTKLPYPRISYDEAIELLKENDFDVDWGVDFGSPEETFLADHFDQPVFV
LNYPKAIKPFYMKPHPTRDDVVICADLLAPEGYGEIIGGSERATDYNYLLDQIKQAGLNPDDYAWYLDLRKYGSVPHAGF
GLGLERFLTWITAEDHVRETIPFPRLLNRIYP

Sequences:

>Translated_432_residues
MVQQINIIDAKDHVDEKVKIGAWLTNKRSSGKIAFLQLRDGSAFFQGVVVKSQVSEEVFELAKEVKQEASMWITGVIHED
SRSKFGYEIEVEDIVVVGESEEYPITPKEHGVDFLLDHRHLWLRSKRPWAIMNIRNEVIRGTYEFFNQEGFIKMDSPILT
GSAPEGTTELFHTEYFDRDAYLSQSGQLYAEAGALAYGKVFTFGPTFRAEKSKTRRHLIEFWMIEPEMAFMHQEQSLEVQ
ERYIAFLVQGVIDHCQYALDILGRDVETLKKYTKLPYPRISYDEAIELLKENDFDVDWGVDFGSPEETFLADHFDQPVFV
LNYPKAIKPFYMKPHPTRDDVVICADLLAPEGYGEIIGGSERATDYNYLLDQIKQAGLNPDDYAWYLDLRKYGSVPHAGF
GLGLERFLTWITAEDHVRETIPFPRLLNRIYP
>Mature_432_residues
MVQQINIIDAKDHVDEKVKIGAWLTNKRSSGKIAFLQLRDGSAFFQGVVVKSQVSEEVFELAKEVKQEASMWITGVIHED
SRSKFGYEIEVEDIVVVGESEEYPITPKEHGVDFLLDHRHLWLRSKRPWAIMNIRNEVIRGTYEFFNQEGFIKMDSPILT
GSAPEGTTELFHTEYFDRDAYLSQSGQLYAEAGALAYGKVFTFGPTFRAEKSKTRRHLIEFWMIEPEMAFMHQEQSLEVQ
ERYIAFLVQGVIDHCQYALDILGRDVETLKKYTKLPYPRISYDEAIELLKENDFDVDWGVDFGSPEETFLADHFDQPVFV
LNYPKAIKPFYMKPHPTRDDVVICADLLAPEGYGEIIGGSERATDYNYLLDQIKQAGLNPDDYAWYLDLRKYGSVPHAGF
GLGLERFLTWITAEDHVRETIPFPRLLNRIYP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI226491502, Length=445, Percent_Identity=34.8314606741573, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI4758762, Length=435, Percent_Identity=32.183908045977, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI45439306, Length=451, Percent_Identity=28.6031042128603, Blast_Score=168, Evalue=7e-42,
Organism=Escherichia coli, GI1787161, Length=463, Percent_Identity=34.5572354211663, Blast_Score=271, Evalue=5e-74,
Organism=Escherichia coli, GI1789256, Length=456, Percent_Identity=23.6842105263158, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788173, Length=253, Percent_Identity=24.1106719367589, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71984122, Length=441, Percent_Identity=34.6938775510204, Blast_Score=244, Evalue=8e-65,
Organism=Caenorhabditis elegans, GI17556372, Length=412, Percent_Identity=31.5533980582524, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17551876, Length=472, Percent_Identity=26.9067796610169, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI32566633, Length=302, Percent_Identity=25.1655629139073, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6321807, Length=447, Percent_Identity=33.7807606263982, Blast_Score=233, Evalue=7e-62,
Organism=Saccharomyces cerevisiae, GI6319872, Length=452, Percent_Identity=34.7345132743363, Blast_Score=232, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6323011, Length=467, Percent_Identity=28.051391862955, Blast_Score=161, Evalue=2e-40,
Organism=Drosophila melanogaster, GI19921528, Length=446, Percent_Identity=33.1838565022422, Blast_Score=228, Evalue=5e-60,
Organism=Drosophila melanogaster, GI19922416, Length=445, Percent_Identity=31.2359550561798, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI17136276, Length=438, Percent_Identity=28.310502283105, Blast_Score=161, Evalue=8e-40,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): SYN2_LACPL (Q88WA8)

Other databases:

- EMBL:   AL935257
- RefSeq:   NP_785313.1
- ProteinModelPortal:   Q88WA8
- SMR:   Q88WA8
- GeneID:   1064108
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1740
- NMPDR:   fig|220668.1.peg.1447
- HOGENOM:   HBG745843
- OMA:   CREDMEF
- ProtClustDB:   PRK03932
- BioCyc:   LPLA220668:LP_1740-MONOMER
- BRENDA:   6.1.1.22
- GO:   GO:0005737
- HAMAP:   MF_00534
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00457

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.22

Molecular weight: Translated: 49868; Mature: 49868

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQQINIIDAKDHVDEKVKIGAWLTNKRSSGKIAFLQLRDGSAFFQGVVVKSQVSEEVFE
CCCEEEEECCCCCCCCCEEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
LAKEVKQEASMWITGVIHEDSRSKFGYEIEVEDIVVVGESEEYPITPKEHGVDFLLDHRH
HHHHHHHHHCEEEEEEEECCCCCCCCEEEEEEEEEEEECCCCCCCCCHHCCCCEEECCCE
LWLRSKRPWAIMNIRNEVIRGTYEFFNQEGFIKMDSPILTGSAPEGTTELFHTEYFDRDA
EEEECCCCEEEEHHHHHHHHHHHHHHCCCCCEEECCCEEECCCCCCCHHHHHHHHCCCHH
YLSQSGQLYAEAGALAYGKVFTFGPTFRAEKSKTRRHLIEFWMIEPEMAFMHQEQSLEVQ
HHCCCCCEEEECCCEEECCEEEECCCCCCHHHHHHHHHHHHHEECCHHHHHHCCCCHHHH
ERYIAFLVQGVIDHCQYALDILGRDVETLKKYTKLPYPRISYDEAIELLKENDFDVDWGV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEECC
DFGSPEETFLADHFDQPVFVLNYPKAIKPFYMKPHPTRDDVVICADLLAPEGYGEIIGGS
CCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHCCCCCCCCCCCC
ERATDYNYLLDQIKQAGLNPDDYAWYLDLRKYGSVPHAGFGLGLERFLTWITAEDHVRET
CCCCHHHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCCCCHHHCHHHHHHHHCCCHHHHHC
IPFPRLLNRIYP
CCHHHHHHHHCC
>Mature Secondary Structure
MVQQINIIDAKDHVDEKVKIGAWLTNKRSSGKIAFLQLRDGSAFFQGVVVKSQVSEEVFE
CCCEEEEECCCCCCCCCEEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
LAKEVKQEASMWITGVIHEDSRSKFGYEIEVEDIVVVGESEEYPITPKEHGVDFLLDHRH
HHHHHHHHHCEEEEEEEECCCCCCCCEEEEEEEEEEEECCCCCCCCCHHCCCCEEECCCE
LWLRSKRPWAIMNIRNEVIRGTYEFFNQEGFIKMDSPILTGSAPEGTTELFHTEYFDRDA
EEEECCCCEEEEHHHHHHHHHHHHHHCCCCCEEECCCEEECCCCCCCHHHHHHHHCCCHH
YLSQSGQLYAEAGALAYGKVFTFGPTFRAEKSKTRRHLIEFWMIEPEMAFMHQEQSLEVQ
HHCCCCCEEEECCCEEECCEEEECCCCCCHHHHHHHHHHHHHEECCHHHHHHCCCCHHHH
ERYIAFLVQGVIDHCQYALDILGRDVETLKKYTKLPYPRISYDEAIELLKENDFDVDWGV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEECC
DFGSPEETFLADHFDQPVFVLNYPKAIKPFYMKPHPTRDDVVICADLLAPEGYGEIIGGS
CCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHCCCCCCCCCCCC
ERATDYNYLLDQIKQAGLNPDDYAWYLDLRKYGSVPHAGFGLGLERFLTWITAEDHVRET
CCCCHHHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCCCCHHHCHHHHHHHHCCCHHHHHC
IPFPRLLNRIYP
CCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566