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The map label for this gene is rsmF [H]

Identifier: 28378408

GI number: 28378408

Start: 1562118

End: 1563485

Strand: Direct

Name: rsmF [H]

Synonym: lp_1724

Alternate gene names: 28378408

Gene position: 1562118-1563485 (Clockwise)

Preceding gene: 28378406

Following gene: 28378409

Centisome position: 47.22

GC content: 50.8

Gene sequence:

>1368_bases
GTGCAATTACCAGCAGAATTTATTCAAAAATATCAACGACTACTAGGCGATCAAGCGCCCGCTTTTTTGGCAGCTTTCGA
TGGCCCTGTTGAAAAAGGGTTTCGGATTAATCCGAACAAGACTGTGACGGCCACGATGCGCGCCAAGATGACAGCACCCG
TTCCGTATAGCCCAATTGGGTATTATGGCAAGGTGAACGGCAATGCGCTGGAACACTTGGCTGGTGCCGTCTACAGTCAA
GAACCAAGTGCCATGACCGTTGGCGAGTTTGCAAAACCAGAACCTGGTGAACGAGTGTTAGACTTATGTGCGGCACCGGG
TGGCAAAACGACGCATCTGTTAAGCTACTTGCATCAGACCGGTCTACTCGTGACAAATGAGATCAATCGGCAACGGGTGA
CCGCCCTTGGCGATAATGTGGAACGCTACGGCGCCCGCAATACAGTGATTACCAACGATACACCCGCCGCCCTTGCCAAG
GAATTGCCAGGCTTTTTTGACCGGATCTTGGTGGATGCGCCGTGTTCGGGTGAAGGGATGTTTCGTAAGGATCATGACGC
AGTTCAGTATTGGACGCCCGACTATCCAGCGGCGTGTGCTGAACGCCAACGGGAGATTTTGACGGAAGCGGTCAAGATGT
TGCGTCCGGGTGGACATTTGATTTATTCGACCTGTACCTTTGCACCGGAAGAGGACGAACAGATGATGGCCTGGCTGTTA
AAAACTTATCCTGAGTTTGAGCTGGAGCCACTGGCCAAGACGGCTGGGATGGTCGATGCAAAGCCGGAATGGGCGGATGG
CAATCCTGAACTGGCTAAAGCGGCCCGGTTATTTCCGCACTTGATGCGCGGTGAGGGTCACTTTATTGCCAAATTGGTCT
ATCATGGCACGACGGTGGGCAAGGACAAAAAAGTGGTTCGCGAACAGTTAAACGCCACGCAACGGCAGCTATGGACGGAC
TTTTTAAAAAAACAAGCAATGGCCGGTTTACCACAGCTCGTGTTACAGGCACATGGTGATCAGTTATACGGCGTGCCAGC
GATGATGCCCGTTACGCGAAAGCTCAAGGTCTTTCGCCCCGGGATTTTATTGGGAACGTTTAAGAAGAAACGGTTTGAAC
CAAGTTACGCGTTAGCCTTAGCGAGTGATGATTTGTCCTTACCCAAAACTGAAATAACGCGTGAACAGTGGGCATTGTAT
GTCCATGGTGAGACATTTGAATTGACAAGTGCGCCTGTAGCTGGTTTTCGGGTCTTAACTTGTGATGGGTTGCCAGTTGG
GTTTGGAAAAATCGTCGCCAAGACGGTTAAAAATTTCTTTCCTAAGGGCTTACGGTTCTTGGCGACCTCTGAAAACGCGA
CGCTCTAA

Upstream 100 bases:

>100_bases
AGCATAAATCTCACCTAATTGCGAATCTTTGCTACCTTACAGTCGGGGCAAAATGTGGTAATCTAATTGAGGATGCTTTT
TAATTGAGGAGGAAATTTGG

Downstream 100 bases:

>100_bases
CCTGACATGATATTGACTAAGTCATAACGTCCGCGTGCGATTGACGCTGAACAGGTGATGAGCAGCGGCCGTCATGAATC
ATTCGATCCGTGGTAAAATC

Product: rRNA methylase (putative)

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MQLPAEFIQKYQRLLGDQAPAFLAAFDGPVEKGFRINPNKTVTATMRAKMTAPVPYSPIGYYGKVNGNALEHLAGAVYSQ
EPSAMTVGEFAKPEPGERVLDLCAAPGGKTTHLLSYLHQTGLLVTNEINRQRVTALGDNVERYGARNTVITNDTPAALAK
ELPGFFDRILVDAPCSGEGMFRKDHDAVQYWTPDYPAACAERQREILTEAVKMLRPGGHLIYSTCTFAPEEDEQMMAWLL
KTYPEFELEPLAKTAGMVDAKPEWADGNPELAKAARLFPHLMRGEGHFIAKLVYHGTTVGKDKKVVREQLNATQRQLWTD
FLKKQAMAGLPQLVLQAHGDQLYGVPAMMPVTRKLKVFRPGILLGTFKKKRFEPSYALALASDDLSLPKTEITREQWALY
VHGETFELTSAPVAGFRVLTCDGLPVGFGKIVAKTVKNFFPKGLRFLATSENATL

Sequences:

>Translated_455_residues
MQLPAEFIQKYQRLLGDQAPAFLAAFDGPVEKGFRINPNKTVTATMRAKMTAPVPYSPIGYYGKVNGNALEHLAGAVYSQ
EPSAMTVGEFAKPEPGERVLDLCAAPGGKTTHLLSYLHQTGLLVTNEINRQRVTALGDNVERYGARNTVITNDTPAALAK
ELPGFFDRILVDAPCSGEGMFRKDHDAVQYWTPDYPAACAERQREILTEAVKMLRPGGHLIYSTCTFAPEEDEQMMAWLL
KTYPEFELEPLAKTAGMVDAKPEWADGNPELAKAARLFPHLMRGEGHFIAKLVYHGTTVGKDKKVVREQLNATQRQLWTD
FLKKQAMAGLPQLVLQAHGDQLYGVPAMMPVTRKLKVFRPGILLGTFKKKRFEPSYALALASDDLSLPKTEITREQWALY
VHGETFELTSAPVAGFRVLTCDGLPVGFGKIVAKTVKNFFPKGLRFLATSENATL
>Mature_455_residues
MQLPAEFIQKYQRLLGDQAPAFLAAFDGPVEKGFRINPNKTVTATMRAKMTAPVPYSPIGYYGKVNGNALEHLAGAVYSQ
EPSAMTVGEFAKPEPGERVLDLCAAPGGKTTHLLSYLHQTGLLVTNEINRQRVTALGDNVERYGARNTVITNDTPAALAK
ELPGFFDRILVDAPCSGEGMFRKDHDAVQYWTPDYPAACAERQREILTEAVKMLRPGGHLIYSTCTFAPEEDEQMMAWLL
KTYPEFELEPLAKTAGMVDAKPEWADGNPELAKAARLFPHLMRGEGHFIAKLVYHGTTVGKDKKVVREQLNATQRQLWTD
FLKKQAMAGLPQLVLQAHGDQLYGVPAMMPVTRKLKVFRPGILLGTFKKKRFEPSYALALASDDLSLPKTEITREQWALY
VHGETFELTSAPVAGFRVLTCDGLPVGFGKIVAKTVKNFFPKGLRFLATSENATL

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=294, Percent_Identity=30.2721088435374, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI76150623, Length=294, Percent_Identity=30.2721088435374, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI32698918, Length=217, Percent_Identity=33.6405529953917, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI39995082, Length=183, Percent_Identity=31.6939890710383, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI301336155, Length=186, Percent_Identity=31.7204301075269, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI11545785, Length=160, Percent_Identity=34.375, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI8922322, Length=184, Percent_Identity=29.3478260869565, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI270288818, Length=184, Percent_Identity=29.3478260869565, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI23199998, Length=184, Percent_Identity=29.3478260869565, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI270288816, Length=183, Percent_Identity=28.9617486338798, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI40316918, Length=154, Percent_Identity=30.5194805194805, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI87081985, Length=462, Percent_Identity=31.3852813852814, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI2367212, Length=276, Percent_Identity=32.9710144927536, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17536757, Length=228, Percent_Identity=35.0877192982456, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25143471, Length=178, Percent_Identity=32.5842696629214, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324268, Length=286, Percent_Identity=31.1188811188811, Blast_Score=126, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6319447, Length=190, Percent_Identity=30.5263157894737, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI22024126, Length=279, Percent_Identity=30.8243727598566, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI21356513, Length=279, Percent_Identity=31.1827956989247, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24668781, Length=205, Percent_Identity=34.6341463414634, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21355201, Length=234, Percent_Identity=29.0598290598291, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19920494, Length=174, Percent_Identity=32.7586206896552, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 50303; Mature: 50303

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLPAEFIQKYQRLLGDQAPAFLAAFDGPVEKGFRINPNKTVTATMRAKMTAPVPYSPIG
CCCCHHHHHHHHHHHCCCCCCEEEECCCHHHCCEEECCCCEEEEEEEEEECCCCCCCCCC
YYGKVNGNALEHLAGAVYSQEPSAMTVGEFAKPEPGERVLDLCAAPGGKTTHLLSYLHQT
CEECCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHC
GLLVTNEINRQRVTALGDNVERYGARNTVITNDTPAALAKELPGFFDRILVDAPCSGEGM
CCEEECCCCHHHHHHHCCCHHHHCCCCCEEECCCHHHHHHHCCHHHHHHHCCCCCCCCCC
FRKDHDAVQYWTPDYPAACAERQREILTEAVKMLRPGGHLIYSTCTFAPEEDEQMMAWLL
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
KTYPEFELEPLAKTAGMVDAKPEWADGNPELAKAARLFPHLMRGEGHFIAKLVYHGTTVG
HHCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCCC
KDKKVVREQLNATQRQLWTDFLKKQAMAGLPQLVLQAHGDQLYGVPAMMPVTRKLKVFRP
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEECCCCCCCHHHHHHHHCC
GILLGTFKKKRFEPSYALALASDDLSLPKTEITREQWALYVHGETFELTSAPVAGFRVLT
CEEEECCHHHHCCCCEEEEEECCCCCCCHHHHCHHHEEEEECCCEEEECCCCCCCEEEEE
CDGLPVGFGKIVAKTVKNFFPKGLRFLATSENATL
ECCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCC
>Mature Secondary Structure
MQLPAEFIQKYQRLLGDQAPAFLAAFDGPVEKGFRINPNKTVTATMRAKMTAPVPYSPIG
CCCCHHHHHHHHHHHCCCCCCEEEECCCHHHCCEEECCCCEEEEEEEEEECCCCCCCCCC
YYGKVNGNALEHLAGAVYSQEPSAMTVGEFAKPEPGERVLDLCAAPGGKTTHLLSYLHQT
CEECCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHC
GLLVTNEINRQRVTALGDNVERYGARNTVITNDTPAALAKELPGFFDRILVDAPCSGEGM
CCEEECCCCHHHHHHHCCCHHHHCCCCCEEECCCHHHHHHHCCHHHHHHHCCCCCCCCCC
FRKDHDAVQYWTPDYPAACAERQREILTEAVKMLRPGGHLIYSTCTFAPEEDEQMMAWLL
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
KTYPEFELEPLAKTAGMVDAKPEWADGNPELAKAARLFPHLMRGEGHFIAKLVYHGTTVG
HHCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCCC
KDKKVVREQLNATQRQLWTDFLKKQAMAGLPQLVLQAHGDQLYGVPAMMPVTRKLKVFRP
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEECCCCCCCHHHHHHHHCC
GILLGTFKKKRFEPSYALALASDDLSLPKTEITREQWALYVHGETFELTSAPVAGFRVLT
CEEEECCHHHHCCCCEEEEEECCCCCCCHHHHCHHHEEEEECCCEEEECCCCCCCEEEEE
CDGLPVGFGKIVAKTVKNFFPKGLRFLATSENATL
ECCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA