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The map label for this gene is fabI [H]

Identifier: 28378371

GI number: 28378371

Start: 1531315

End: 1532073

Strand: Direct

Name: fabI [H]

Synonym: lp_1681

Alternate gene names: 28378371

Gene position: 1531315-1532073 (Clockwise)

Preceding gene: 28378370

Following gene: 28378372

Centisome position: 46.29

GC content: 47.04

Gene sequence:

>759_bases
ATGGATGGAATTTTATCGGGTAAGACCATTGTGGTCATGGGTGTGGCCAATCAGCGCAGTATTGCCTGGGGGTGTACCGA
GGCATTAATTGCACAGGGGGCCCAGGTTATCTTGACTTACCAAAATGACCGTTTGAAGCAAAGCTTACAACGGTTTGTTG
CGCCAGATGTGCCGTTAATTGCCTGTGATGTTGCTGATGATGACAATGTTGAGCGGGCATTTGCAAGCATTAAACAACAG
TATGGTGCCATCGATGGGATTATCCATGCGATTGCTTATGCGGATAAAGCAACCTTAGAAGGTGATTTTGTGAATACCAC
GAAAGCTGGATATGATTTGGCACAAAATATTAGTGCGTATTCGCTGATTGCAGTTGCCCGAGCAGCTCGGCCAATGCTGA
AACCAGGAGCCAGTCTCGTAACGTTGACGTATTTTGGATCAGAGCGAGCCGTACCAAATTACAATATGATGGGGGTTGCT
AAGGCCGCGTTGGAAGCAAATGTGCGTTACTTGGCGCGTGACCTTGGACCACAACAAGTCCGCGTGAATGCAATTTCAGC
CGGAGCAGTCAAAACGTTGGCGGTAACGGGTATTCATGAGCATCAGCAATTATTAAAATTATCTCGCAGTATGACAGTTG
ATGGAGAACCGGTAAAAACGCGTGAGATCGGCAACGTGGCTGCCTTTTTATTAAGCAATCTATCGACTGGAATGACCGGG
GACGTGGTATACGTGGATAAAGGGGTCCACTTAAGTTAA

Upstream 100 bases:

>100_bases
ACGAGACATTAACTGCTTTAACAGCAAAGTCGGTGGCGGAACTGGTTACGCAACGCCAAGCCCGTTATCGGCAATTTTAA
ATAAGTGAGGAATGATAAGT

Downstream 100 bases:

>100_bases
TGCCAATCACTCAAGTTGTCTTTAAACGGCAGTGGCTTCAGATGCCGGTCGATGTTTCTAAAAAAATGCGGCGGGTCACT
CAGCGAACCGTGAGTCGCCA

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: Cold shock-induced protein 15; CSI15; NADH-dependent enoyl-ACP reductase; Vegetative protein 241; VEG241 [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MDGILSGKTIVVMGVANQRSIAWGCTEALIAQGAQVILTYQNDRLKQSLQRFVAPDVPLIACDVADDDNVERAFASIKQQ
YGAIDGIIHAIAYADKATLEGDFVNTTKAGYDLAQNISAYSLIAVARAARPMLKPGASLVTLTYFGSERAVPNYNMMGVA
KAALEANVRYLARDLGPQQVRVNAISAGAVKTLAVTGIHEHQQLLKLSRSMTVDGEPVKTREIGNVAAFLLSNLSTGMTG
DVVYVDKGVHLS

Sequences:

>Translated_252_residues
MDGILSGKTIVVMGVANQRSIAWGCTEALIAQGAQVILTYQNDRLKQSLQRFVAPDVPLIACDVADDDNVERAFASIKQQ
YGAIDGIIHAIAYADKATLEGDFVNTTKAGYDLAQNISAYSLIAVARAARPMLKPGASLVTLTYFGSERAVPNYNMMGVA
KAALEANVRYLARDLGPQQVRVNAISAGAVKTLAVTGIHEHQQLLKLSRSMTVDGEPVKTREIGNVAAFLLSNLSTGMTG
DVVYVDKGVHLS
>Mature_252_residues
MDGILSGKTIVVMGVANQRSIAWGCTEALIAQGAQVILTYQNDRLKQSLQRFVAPDVPLIACDVADDDNVERAFASIKQQ
YGAIDGIIHAIAYADKATLEGDFVNTTKAGYDLAQNISAYSLIAVARAARPMLKPGASLVTLTYFGSERAVPNYNMMGVA
KAALEANVRYLARDLGPQQVRVNAISAGAVKTLAVTGIHEHQQLLKLSRSMTVDGEPVKTREIGNVAAFLLSNLSTGMTG
DVVYVDKGVHLS

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI7705925, Length=254, Percent_Identity=28.740157480315, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1787545, Length=254, Percent_Identity=46.8503937007874, Blast_Score=246, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17562906, Length=272, Percent_Identity=28.6764705882353, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71994604, Length=202, Percent_Identity=25.2475247524752, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI21357041, Length=253, Percent_Identity=24.901185770751, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24644339, Length=201, Percent_Identity=23.8805970149254, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 26881; Mature: 26881

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGILSGKTIVVMGVANQRSIAWGCTEALIAQGAQVILTYQNDRLKQSLQRFVAPDVPLI
CCCCCCCCEEEEEEECCCCCEEHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCEE
ACDVADDDNVERAFASIKQQYGAIDGIIHAIAYADKATLEGDFVNTTKAGYDLAQNISAY
EEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHH
SLIAVARAARPMLKPGASLVTLTYFGSERAVPNYNMMGVAKAALEANVRYLARDLGPQQV
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
RVNAISAGAVKTLAVTGIHEHQQLLKLSRSMTVDGEPVKTREIGNVAAFLLSNLSTGMTG
EEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
DVVYVDKGVHLS
CEEEEECCCCCC
>Mature Secondary Structure
MDGILSGKTIVVMGVANQRSIAWGCTEALIAQGAQVILTYQNDRLKQSLQRFVAPDVPLI
CCCCCCCCEEEEEEECCCCCEEHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCEE
ACDVADDDNVERAFASIKQQYGAIDGIIHAIAYADKATLEGDFVNTTKAGYDLAQNISAY
EEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHH
SLIAVARAARPMLKPGASLVTLTYFGSERAVPNYNMMGVAKAALEANVRYLARDLGPQQV
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
RVNAISAGAVKTLAVTGIHEHQQLLKLSRSMTVDGEPVKTREIGNVAAFLLSNLSTGMTG
EEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
DVVYVDKGVHLS
CEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 8755892; 9298659 [H]