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The map label for this gene is miaA

Identifier: 28378283

GI number: 28378283

Start: 1442079

End: 1443014

Strand: Direct

Name: miaA

Synonym: lp_1579

Alternate gene names: 28378283

Gene position: 1442079-1443014 (Clockwise)

Preceding gene: 28378282

Following gene: 28378284

Centisome position: 43.59

GC content: 50.32

Gene sequence:

>936_bases
GTGACAACAGTAACTAAACATAAAGTATTACTAATCGCGGGACCAACAGCAGTCGGTAAGACGGCATTATCGCTAGCGCT
GGCTAAACAGCTGAATGGTGAAATCATTTCAGGAGACTCAATGCAGGTCTATCGTCACTTGGATATTGGTACGGCTAAAA
TAATGCCGGAAGAGCAGGCGGGCATTCCCCACCATCTAATCGATATCAAAAATATTGATCAGCGTTTTACAGTCGCTGAA
TTTGTCAGTCGGACGACGGCGTTGATCATTGATATTAGTGCTCGGGGTAAACTGCCTATTATTGTGGGTGGAACTGGCTT
TTACTTGCAGTCCTTGTTGGCGGGCTATCAGTTTGGACCAGCGGATAATGAACCGGATATGGCGTATCGTCAAGCCTGGT
TTGATCGTGCCGCTGTCGAAGGTTCGGATGTGGCGTGGATGGCGTTGAAACAGCGTGATCCGCAGGCGGCCACCGCAATT
GCGCCCGCTAACTTGGTACGGGTCGTACGGGCGTTGGAGTACGTGCATACAACTGGTCAGCTTTTTTCTGAGCAGGCGGA
TACGCACGGTGACACACTGGATGCTTATACCTTATGTCTGACCGCGGAGCGAGCGCTGCTGTATACTCGGATCAATCAAC
GTGTCGATCAAATGGTAGCGGCCGGATTGGAACAAGAGGCGCGGTGGCTATTTGACCAAGGTGGCGCCATGTTACCGGCT
GGTAAGGGGATCGGGTATCACGAATGGTTCCCTTACTTTAATGGGGAGCAGACGCGTGACGAATCAATTGCTAAGATCAA
ACAGGACTCACGTCGTTATGCGAAGCGTCAGTTGACTTGGTTCCGTAACAAGATGTCGGTCGACTGGATCAATCTATTGG
AACACCCTGAATTACGGGCCTCGATTGACCAACGGCTAGCTAGTTGGTTAAGCTAA

Upstream 100 bases:

>100_bases
GATATGCGGTTCTGTTATCGTCATCGGTTTGCGGGACTGATTACCGACTACCCTGGATTAGCACGAAGAATTCGTAAGCA
GGTACAAGGAGGTTAAACGA

Downstream 100 bases:

>100_bases
ATTATAAATTGGTATACCTATGATAAAACGTGTGATATTTTCTAACATGAATGATTGACATTCGATGATAACATTGCTAA
TATTAAGTCTGTATTAAAGA

Product: tRNA isopentenyltransferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTTVTKHKVLLIAGPTAVGKTALSLALAKQLNGEIISGDSMQVYRHLDIGTAKIMPEEQAGIPHHLIDIKNIDQRFTVAE
FVSRTTALIIDISARGKLPIIVGGTGFYLQSLLAGYQFGPADNEPDMAYRQAWFDRAAVEGSDVAWMALKQRDPQAATAI
APANLVRVVRALEYVHTTGQLFSEQADTHGDTLDAYTLCLTAERALLYTRINQRVDQMVAAGLEQEARWLFDQGGAMLPA
GKGIGYHEWFPYFNGEQTRDESIAKIKQDSRRYAKRQLTWFRNKMSVDWINLLEHPELRASIDQRLASWLS

Sequences:

>Translated_311_residues
MTTVTKHKVLLIAGPTAVGKTALSLALAKQLNGEIISGDSMQVYRHLDIGTAKIMPEEQAGIPHHLIDIKNIDQRFTVAE
FVSRTTALIIDISARGKLPIIVGGTGFYLQSLLAGYQFGPADNEPDMAYRQAWFDRAAVEGSDVAWMALKQRDPQAATAI
APANLVRVVRALEYVHTTGQLFSEQADTHGDTLDAYTLCLTAERALLYTRINQRVDQMVAAGLEQEARWLFDQGGAMLPA
GKGIGYHEWFPYFNGEQTRDESIAKIKQDSRRYAKRQLTWFRNKMSVDWINLLEHPELRASIDQRLASWLS
>Mature_310_residues
TTVTKHKVLLIAGPTAVGKTALSLALAKQLNGEIISGDSMQVYRHLDIGTAKIMPEEQAGIPHHLIDIKNIDQRFTVAEF
VSRTTALIIDISARGKLPIIVGGTGFYLQSLLAGYQFGPADNEPDMAYRQAWFDRAAVEGSDVAWMALKQRDPQAATAIA
PANLVRVVRALEYVHTTGQLFSEQADTHGDTLDAYTLCLTAERALLYTRINQRVDQMVAAGLEQEARWLFDQGGAMLPAG
KGIGYHEWFPYFNGEQTRDESIAKIKQDSRRYAKRQLTWFRNKMSVDWINLLEHPELRASIDQRLASWLS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=318, Percent_Identity=32.3899371069182, Blast_Score=142, Evalue=5e-34,
Organism=Escherichia coli, GI1790613, Length=305, Percent_Identity=37.7049180327869, Blast_Score=181, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI25144712, Length=300, Percent_Identity=29.6666666666667, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324848, Length=296, Percent_Identity=33.7837837837838, Blast_Score=157, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24649830, Length=338, Percent_Identity=31.6568047337278, Blast_Score=141, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_LACPL (Q88WP5)

Other databases:

- EMBL:   AL935256
- RefSeq:   NP_785175.1
- ProteinModelPortal:   Q88WP5
- SMR:   Q88WP5
- GeneID:   1061495
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1579
- NMPDR:   fig|220668.1.peg.1309
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   CLSK2520228
- BioCyc:   LPLA220668:LP_1579-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34730; Mature: 34599

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVTKHKVLLIAGPTAVGKTALSLALAKQLNGEIISGDSMQVYRHLDIGTAKIMPEEQA
CCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEECCCCEEEEEEECCCHHCCCCCHHC
GIPHHLIDIKNIDQRFTVAEFVSRTTALIIDISARGKLPIIVGGTGFYLQSLLAGYQFGP
CCCCEEEECCCCCHHHHHHHHHHCCEEEEEEEECCCCEEEEECCHHHHHHHHHHCCCCCC
ADNEPDMAYRQAWFDRAAVEGSDVAWMALKQRDPQAATAIAPANLVRVVRALEYVHTTGQ
CCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LFSEQADTHGDTLDAYTLCLTAERALLYTRINQRVDQMVAAGLEQEARWLFDQGGAMLPA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCEECCC
GKGIGYHEWFPYFNGEQTRDESIAKIKQDSRRYAKRQLTWFRNKMSVDWINLLEHPELRA
CCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHH
SIDQRLASWLS
HHHHHHHHHCC
>Mature Secondary Structure 
TTVTKHKVLLIAGPTAVGKTALSLALAKQLNGEIISGDSMQVYRHLDIGTAKIMPEEQA
CCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEECCCCEEEEEEECCCHHCCCCCHHC
GIPHHLIDIKNIDQRFTVAEFVSRTTALIIDISARGKLPIIVGGTGFYLQSLLAGYQFGP
CCCCEEEECCCCCHHHHHHHHHHCCEEEEEEEECCCCEEEEECCHHHHHHHHHHCCCCCC
ADNEPDMAYRQAWFDRAAVEGSDVAWMALKQRDPQAATAIAPANLVRVVRALEYVHTTGQ
CCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LFSEQADTHGDTLDAYTLCLTAERALLYTRINQRVDQMVAAGLEQEARWLFDQGGAMLPA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCEECCC
GKGIGYHEWFPYFNGEQTRDESIAKIKQDSRRYAKRQLTWFRNKMSVDWINLLEHPELRA
CCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHH
SIDQRLASWLS
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566