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The map label for this gene is greA

Identifier: 28378269

GI number: 28378269

Start: 1428800

End: 1429282

Strand: Direct

Name: greA

Synonym: lp_1563

Alternate gene names: 28378269

Gene position: 1428800-1429282 (Clockwise)

Preceding gene: 28378268

Following gene: 28378272

Centisome position: 43.19

GC content: 41.2

Gene sequence:

>483_bases
TTGGCTGAAGATAAGACATATCCAATGACAGAAGAAGGCAAGGTCAAGCTGGAAAAAGAGCTTGAAGACCTTCGAATTAA
TCAACGTCCAGAAATTATTAATCGTATTAAGATCGCACGTAGCTACGGTGATTTATCAGAAAACTCTGAGTATGAATCAG
CAAAGAATGAACAAAGCTTGCTAGAAAACCGGATTAAGACGGTTGAACACATGCTTCAATATGCTGAAATCATCGATAGT
GACAAGATTGACGAAACGGAAGTCTCCGTTGGTAAAATCGTGACGTTTAAAGAATTACCTGATGAAGAACCAGAAAGCTA
CACCATTGTTGGTGCTGCCGAAGCTGATCCAATGGTTGGCAAGATTTCTAATGATTCCCCGATCGCCAAGGGCTTGATTG
GGCATCACGTTGATGAAGAAGTTGCCATCAACATTCCAGCCGGTACCATGACGGTTAAAATTTTGAAGGTTGAAAACGCG
TAA

Upstream 100 bases:

>100_bases
CTTAACCAGCGCTATTTTAGATTTAAGAGTTTACTTTTAAAATCTGAAATGGTAGGCTGGTTTTTGATAACTATATTGCG
TATTTGAGGAGTGAAACACA

Downstream 100 bases:

>100_bases
TTTTATGCTTAAGACTGTTTTGAGGTAGCGGATACCTTGAAACAGTCTTTTTTGGTGGTTTAGAAGCGCGATTACTGATT
ACCAGACACTATTTAACGAA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA 2

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MAEDKTYPMTEEGKVKLEKELEDLRINQRPEIINRIKIARSYGDLSENSEYESAKNEQSLLENRIKTVEHMLQYAEIIDS
DKIDETEVSVGKIVTFKELPDEEPESYTIVGAAEADPMVGKISNDSPIAKGLIGHHVDEEVAINIPAGTMTVKILKVENA

Sequences:

>Translated_160_residues
MAEDKTYPMTEEGKVKLEKELEDLRINQRPEIINRIKIARSYGDLSENSEYESAKNEQSLLENRIKTVEHMLQYAEIIDS
DKIDETEVSVGKIVTFKELPDEEPESYTIVGAAEADPMVGKISNDSPIAKGLIGHHVDEEVAINIPAGTMTVKILKVENA
>Mature_159_residues
AEDKTYPMTEEGKVKLEKELEDLRINQRPEIINRIKIARSYGDLSENSEYESAKNEQSLLENRIKTVEHMLQYAEIIDSD
KIDETEVSVGKIVTFKELPDEEPESYTIVGAAEADPMVGKISNDSPIAKGLIGHHVDEEVAINIPAGTMTVKILKVENA

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=155, Percent_Identity=43.2258064516129, Blast_Score=113, Evalue=7e-27,
Organism=Escherichia coli, GI87082261, Length=154, Percent_Identity=31.8181818181818, Blast_Score=71, Evalue=3e-14,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA2_LACPL (Q88WQ9)

Other databases:

- EMBL:   AL935256
- RefSeq:   NP_785161.1
- ProteinModelPortal:   Q88WQ9
- SMR:   Q88WQ9
- GeneID:   1061434
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1563
- NMPDR:   fig|220668.1.peg.1295
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   LPLA220668:LP_1563-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17914; Mature: 17783

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEDKTYPMTEEGKVKLEKELEDLRINQRPEIINRIKIARSYGDLSENSEYESAKNEQSL
CCCCCCCCCCCCCCEEHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LENRIKTVEHMLQYAEIIDSDKIDETEVSVGKIVTFKELPDEEPESYTIVGAAEADPMVG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCEE
KISNDSPIAKGLIGHHVDEEVAINIPAGTMTVKILKVENA
ECCCCCCHHHHHHHCCCCCCEEEECCCCCEEEEEEEECCC
>Mature Secondary Structure 
AEDKTYPMTEEGKVKLEKELEDLRINQRPEIINRIKIARSYGDLSENSEYESAKNEQSL
CCCCCCCCCCCCCEEHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LENRIKTVEHMLQYAEIIDSDKIDETEVSVGKIVTFKELPDEEPESYTIVGAAEADPMVG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCEE
KISNDSPIAKGLIGHHVDEEVAINIPAGTMTVKILKVENA
ECCCCCCHHHHHHHCCCCCCEEEECCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566