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The map label for this gene is thrS

Identifier: 28378228

GI number: 28378228

Start: 1385839

End: 1387803

Strand: Direct

Name: thrS

Synonym: lp_1514

Alternate gene names: 28378228

Gene position: 1385839-1387803 (Clockwise)

Preceding gene: 28378227

Following gene: 28378229

Centisome position: 41.89

GC content: 45.19

Gene sequence:

>1965_bases
ATGGCAAGTATTAATGTTAAGTTCCCAGATGGTGCTGAAAAGCAATTTGATGCCGGCGTAACGACGGAAGCAATTGCAAA
GTCAATTAGTCCTTCATTGGCTAAACGGTCGGTTGCTGGTAAGTTCAACGACCAGATCGTGGACTACCGCCAACCACTGA
CAACCGATGGTAGTATCGAAATTATCGCTGCTGATAGTGAAGACGGCCTCAATGTTTTACGGCAAACGGCTGCCCAAGTA
TTAGCTAACGCGGGTAAACAATTATTCCCTAACATTCATTTTGGGAGTGGTGAAGGGAATGCCAACGGTTTCTTCTTTGA
TACCGACAATCCTGACGAAGACGGTAAACAAGTCAGTGAAGATGATTTGGAAGCCATCAGTGATAAGATGGCTGCAATTA
TCAAACAAGATTTACCAATCGAACGCGAAGTCTTATCAAAGGCTGACGCTTTAGCTTTAGTTGGTGACAACCCTTACCAA
CAAGACTTAGTTAACGAACGTGCCGCTGCCAACAATGATCAAGTCGTTGTTTACAAGCAAGGGGACTTCGTTGATTTATC
TGACGGTGCCCAATTAGCTTCAACGGGGAAAGTCAAGGTCTTCAAGTTATTATCCGTTGCCGGTGCATACTGGCAAGGTA
AGTCCAGCAATCCAATGTTACAACGAATCTACGGGACGGCCTTCTTAAAGCAAAAAGACTTAGATGCTGACCTCAAGCGG
CGTCAAGAAGCCCGCGAACGTGATCACCGGGTCATTGGTAACGAATTAGACTTATTCTTCGTTGATCCTAAGGTCGGGAA
TGGCTTGCCTTACTGGATGCCAAATGGTGCAACGATTCGTCGCCAAATCGAACGTTACATTATCGACAAGGAAGTTGCAA
ATGGCTACCAACATGTATACACGCCAGTTTTAGCTAACTTGGACGTTTACAAGCAATCTGGTCACTGGGACCACTACCGT
GAGGACATGTTCCCACCAATGGACATGGGTGACGGCGAACAATTGGAATTACGGCCGATGAACTGCCCATCACATATCCA
GATTTACAACCATCACATTCGCTCATACCGTGAATTACCATTGCGGATCGCTGAACTCGGCATGATGCACCGTTACGAAA
AGTCTGGCGCCTTAACTGGATTATCACGGGTTCGTGAAATGACTTTGAACGATGGTCATACCTTCGTTGCGTTGGATCAA
ATTGAAGAAGAATTCAAAAAAATCTTGAGTTTGATGGTTGAAGTATACGAAGACTTTGACATTTCTGATTACCGGTTCCG
TTTGAGCTACCGTGATCCTGAAAACAAGGAAAAGTACTTCGATGATGACGCTATGTGGGAACGCAGTCAAAAGATGTTGA
AGTCCGCTATGGATGATATGGGCTTGGATTACTTCGAAGCTGAAGGTGAAGCTGCTTTCTATGGTCCTAAGTTGGATGTT
CAAACTAAGACGGCCCTTGGTGGTGAAGAAACCTTATCAACTATCCAATTGGACTTCTTGTTACCAGAACGCTTTGACTT
GAAGTACGTTGGTGCTGATGGTGAAGAACATCGTCCAGTTATGATTCACCGTGGTCTTGTTTCAACGATGGAACGGTTCG
TCGCTTACTTAACTGAAATTTACAAGGGTGCTTTCCCAACCTGGTTAGCACCTAAGCAAGTAACAATCATCCCAGTTAAC
AATGGTGCGCACGGTGCCTACGCTGAAACCGTTCGTCGTCGTTTGGCAGCTGAAGGTGTTCGGGTAAGTATCGATGACCG
TAACGAAAAGATGGGTTACAAGATTCGCGAATCACAAACGAAGAAGGTTCCATACTTGTTAGTTGTCGGTGATCAAGAAG
TTGCTAACGGGAGTGTTTCAGTTCGGAAGTACGGTGAAGAACGGACTGAATCCGAAGCCGTTGACATGTTCATTGGTGCC
ATCACGCAAGAAATCAAGCATTACAGTCGTGGTGCAAGCAAATAA

Upstream 100 bases:

>100_bases
GACGTCCCTAATGCTAGTTCAGTCTGGCATTAGGGATTTTTTTGTCGTCCCGGCGCGGTGTTTTGGTGAGTGGGTAAATT
TTAAGGAGAGTGTCAAGTAT

Downstream 100 bases:

>100_bases
TTAACAGCCAATGGTATCAATATTGATTGATGGGTTGACAAGGGCCGTGGTTCTTGTTAATATATTGGAGTTGAGAAAAA
AAGCAGAAGCACCCGCTTCT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 654; Mature: 653

Protein sequence:

>654_residues
MASINVKFPDGAEKQFDAGVTTEAIAKSISPSLAKRSVAGKFNDQIVDYRQPLTTDGSIEIIAADSEDGLNVLRQTAAQV
LANAGKQLFPNIHFGSGEGNANGFFFDTDNPDEDGKQVSEDDLEAISDKMAAIIKQDLPIEREVLSKADALALVGDNPYQ
QDLVNERAAANNDQVVVYKQGDFVDLSDGAQLASTGKVKVFKLLSVAGAYWQGKSSNPMLQRIYGTAFLKQKDLDADLKR
RQEARERDHRVIGNELDLFFVDPKVGNGLPYWMPNGATIRRQIERYIIDKEVANGYQHVYTPVLANLDVYKQSGHWDHYR
EDMFPPMDMGDGEQLELRPMNCPSHIQIYNHHIRSYRELPLRIAELGMMHRYEKSGALTGLSRVREMTLNDGHTFVALDQ
IEEEFKKILSLMVEVYEDFDISDYRFRLSYRDPENKEKYFDDDAMWERSQKMLKSAMDDMGLDYFEAEGEAAFYGPKLDV
QTKTALGGEETLSTIQLDFLLPERFDLKYVGADGEEHRPVMIHRGLVSTMERFVAYLTEIYKGAFPTWLAPKQVTIIPVN
NGAHGAYAETVRRRLAAEGVRVSIDDRNEKMGYKIRESQTKKVPYLLVVGDQEVANGSVSVRKYGEERTESEAVDMFIGA
ITQEIKHYSRGASK

Sequences:

>Translated_654_residues
MASINVKFPDGAEKQFDAGVTTEAIAKSISPSLAKRSVAGKFNDQIVDYRQPLTTDGSIEIIAADSEDGLNVLRQTAAQV
LANAGKQLFPNIHFGSGEGNANGFFFDTDNPDEDGKQVSEDDLEAISDKMAAIIKQDLPIEREVLSKADALALVGDNPYQ
QDLVNERAAANNDQVVVYKQGDFVDLSDGAQLASTGKVKVFKLLSVAGAYWQGKSSNPMLQRIYGTAFLKQKDLDADLKR
RQEARERDHRVIGNELDLFFVDPKVGNGLPYWMPNGATIRRQIERYIIDKEVANGYQHVYTPVLANLDVYKQSGHWDHYR
EDMFPPMDMGDGEQLELRPMNCPSHIQIYNHHIRSYRELPLRIAELGMMHRYEKSGALTGLSRVREMTLNDGHTFVALDQ
IEEEFKKILSLMVEVYEDFDISDYRFRLSYRDPENKEKYFDDDAMWERSQKMLKSAMDDMGLDYFEAEGEAAFYGPKLDV
QTKTALGGEETLSTIQLDFLLPERFDLKYVGADGEEHRPVMIHRGLVSTMERFVAYLTEIYKGAFPTWLAPKQVTIIPVN
NGAHGAYAETVRRRLAAEGVRVSIDDRNEKMGYKIRESQTKKVPYLLVVGDQEVANGSVSVRKYGEERTESEAVDMFIGA
ITQEIKHYSRGASK
>Mature_653_residues
ASINVKFPDGAEKQFDAGVTTEAIAKSISPSLAKRSVAGKFNDQIVDYRQPLTTDGSIEIIAADSEDGLNVLRQTAAQVL
ANAGKQLFPNIHFGSGEGNANGFFFDTDNPDEDGKQVSEDDLEAISDKMAAIIKQDLPIEREVLSKADALALVGDNPYQQ
DLVNERAAANNDQVVVYKQGDFVDLSDGAQLASTGKVKVFKLLSVAGAYWQGKSSNPMLQRIYGTAFLKQKDLDADLKRR
QEARERDHRVIGNELDLFFVDPKVGNGLPYWMPNGATIRRQIERYIIDKEVANGYQHVYTPVLANLDVYKQSGHWDHYRE
DMFPPMDMGDGEQLELRPMNCPSHIQIYNHHIRSYRELPLRIAELGMMHRYEKSGALTGLSRVREMTLNDGHTFVALDQI
EEEFKKILSLMVEVYEDFDISDYRFRLSYRDPENKEKYFDDDAMWERSQKMLKSAMDDMGLDYFEAEGEAAFYGPKLDVQ
TKTALGGEETLSTIQLDFLLPERFDLKYVGADGEEHRPVMIHRGLVSTMERFVAYLTEIYKGAFPTWLAPKQVTIIPVNN
GAHGAYAETVRRRLAAEGVRVSIDDRNEKMGYKIRESQTKKVPYLLVVGDQEVANGSVSVRKYGEERTESEAVDMFIGAI
TQEIKHYSRGASK

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=641, Percent_Identity=36.6614664586583, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI38570111, Length=622, Percent_Identity=36.4951768488746, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI20070344, Length=644, Percent_Identity=33.8509316770186, Blast_Score=346, Evalue=5e-95,
Organism=Escherichia coli, GI1788013, Length=646, Percent_Identity=36.8421052631579, Blast_Score=452, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI71984184, Length=621, Percent_Identity=33.9774557165861, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71984192, Length=621, Percent_Identity=33.9774557165861, Blast_Score=380, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6322112, Length=634, Percent_Identity=35.0157728706625, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6322654, Length=421, Percent_Identity=35.3919239904988, Blast_Score=248, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24583842, Length=623, Percent_Identity=36.5971107544141, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI28574141, Length=623, Percent_Identity=36.5971107544141, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24583839, Length=623, Percent_Identity=36.5971107544141, Blast_Score=404, Evalue=1e-113,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_LACPL (Q88WU9)

Other databases:

- EMBL:   AL935256
- RefSeq:   NP_785120.1
- ProteinModelPortal:   Q88WU9
- SMR:   Q88WU9
- GeneID:   1064188
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1514
- NMPDR:   fig|220668.1.peg.1254
- HOGENOM:   HBG352811
- OMA:   LFVTPDQ
- ProtClustDB:   PRK00413
- BioCyc:   LPLA220668:LP_1514-MONOMER
- BRENDA:   6.1.1.3
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 73778; Mature: 73647

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASINVKFPDGAEKQFDAGVTTEAIAKSISPSLAKRSVAGKFNDQIVDYRQPLTTDGSIE
CCEEEEECCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCHHHHHHCCCCCCCCCEE
IIAADSEDGLNVLRQTAAQVLANAGKQLFPNIHFGSGEGNANGFFFDTDNPDEDGKQVSE
EEEECCCCHHHHHHHHHHHHHHHCCHHHCCCCEECCCCCCCCEEEEECCCCCCCCCCCCH
DDLEAISDKMAAIIKQDLPIEREVLSKADALALVGDNPYQQDLVNERAAANNDQVVVYKQ
HHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEC
GDFVDLSDGAQLASTGKVKVFKLLSVAGAYWQGKSSNPMLQRIYGTAFLKQKDLDADLKR
CCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHH
RQEARERDHRVIGNELDLFFVDPKVGNGLPYWMPNGATIRRQIERYIIDKEVANGYQHVY
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHH
TPVLANLDVYKQSGHWDHYREDMFPPMDMGDGEQLELRPMNCPSHIQIYNHHIRSYRELP
HHHHHCCHHEECCCCCCHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
LRIAELGMMHRYEKSGALTGLSRVREMTLNDGHTFVALDQIEEEFKKILSLMVEVYEDFD
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC
ISDYRFRLSYRDPENKEKYFDDDAMWERSQKMLKSAMDDMGLDYFEAEGEAAFYGPKLDV
CCCEEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCC
QTKTALGGEETLSTIQLDFLLPERFDLKYVGADGEEHRPVMIHRGLVSTMERFVAYLTEI
EEHHCCCCHHHHHEEEEEEECCCCCCEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHH
YKGAFPTWLAPKQVTIIPVNNGAHGAYAETVRRRLAAEGVRVSIDDRNEKMGYKIRESQT
HHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCCC
KKVPYLLVVGDQEVANGSVSVRKYGEERTESEAVDMFIGAITQEIKHYSRGASK
CCCCEEEEECCCHHCCCCEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ASINVKFPDGAEKQFDAGVTTEAIAKSISPSLAKRSVAGKFNDQIVDYRQPLTTDGSIE
CEEEEECCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCHHHHHHCCCCCCCCCEE
IIAADSEDGLNVLRQTAAQVLANAGKQLFPNIHFGSGEGNANGFFFDTDNPDEDGKQVSE
EEEECCCCHHHHHHHHHHHHHHHCCHHHCCCCEECCCCCCCCEEEEECCCCCCCCCCCCH
DDLEAISDKMAAIIKQDLPIEREVLSKADALALVGDNPYQQDLVNERAAANNDQVVVYKQ
HHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEC
GDFVDLSDGAQLASTGKVKVFKLLSVAGAYWQGKSSNPMLQRIYGTAFLKQKDLDADLKR
CCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHH
RQEARERDHRVIGNELDLFFVDPKVGNGLPYWMPNGATIRRQIERYIIDKEVANGYQHVY
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHH
TPVLANLDVYKQSGHWDHYREDMFPPMDMGDGEQLELRPMNCPSHIQIYNHHIRSYRELP
HHHHHCCHHEECCCCCCHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
LRIAELGMMHRYEKSGALTGLSRVREMTLNDGHTFVALDQIEEEFKKILSLMVEVYEDFD
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC
ISDYRFRLSYRDPENKEKYFDDDAMWERSQKMLKSAMDDMGLDYFEAEGEAAFYGPKLDV
CCCEEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCC
QTKTALGGEETLSTIQLDFLLPERFDLKYVGADGEEHRPVMIHRGLVSTMERFVAYLTEI
EEHHCCCCHHHHHEEEEEEECCCCCCEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHH
YKGAFPTWLAPKQVTIIPVNNGAHGAYAETVRRRLAAEGVRVSIDDRNEKMGYKIRESQT
HHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCCC
KKVPYLLVVGDQEVANGSVSVRKYGEERTESEAVDMFIGAITQEIKHYSRGASK
CCCCEEEEECCCHHCCCCEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566