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The map label for this gene is ribH

Identifier: 28378160

GI number: 28378160

Start: 1317658

End: 1318137

Strand: Direct

Name: ribH

Synonym: lp_1438

Alternate gene names: 28378160

Gene position: 1317658-1318137 (Clockwise)

Preceding gene: 28378159

Following gene: 28378163

Centisome position: 39.83

GC content: 48.96

Gene sequence:

>480_bases
ATGACAACTTTTAACGGCAAAATTAATGGCAACGGGCTTAAGATTGGGATTGCCGTGGCGCGTTTCAATGCGTTTGTAAC
GCAGCAACTTTTAACTGGGGCACAAGAAAGTTTGGTACAGCATGGGGTCAATGAGACCGATATTGACGTGGCCTGGGTAC
CGGGAGCTTTTGAGATTCCACTGACGGTTCAAAAAATGATTACAACCAAGCGCTATGATGGCGTGATTGCACTTGGGGCA
GTCATTCGGGGTGCAACCGCGCACTTTGACTATGTTTGTAGTGGCGTGACGACGGGGATTGCGACCGTCAGTTTGGCAAC
CGATACGCCCATAATGTTCGGCGTTTTAACCACGGATACCATTGAACAAGCTATGGATCGCGCTGGCTTTAAGAGTGGCA
ATAAGGGTGCTGACTGTGCGGTCAGTTTGTTAGAAACGTTGGACGTGCAGCGGGCAATATTAAAAGCTGACTTGGCCTAA

Upstream 100 bases:

>100_bases
GGGTCCCCTTAGAAATACCAGCCAATCAGTACGATCAAGCTTACTTAAAAACAAAACGTGATCGATTTCATCATCAACTC
ACACTAACGGAGGGACACGA

Downstream 100 bases:

>100_bases
ATGACGTAAATTAAGGGTATGCATAATATTTCAAATACCATCAATTTGTAACTTGGGCCATTGTTGATGCCACTGCTTCT
GTGCCATTCGCTGCTGATAG

Product: riboflavin synthase, beta chain

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MTTFNGKINGNGLKIGIAVARFNAFVTQQLLTGAQESLVQHGVNETDIDVAWVPGAFEIPLTVQKMITTKRYDGVIALGA
VIRGATAHFDYVCSGVTTGIATVSLATDTPIMFGVLTTDTIEQAMDRAGFKSGNKGADCAVSLLETLDVQRAILKADLA

Sequences:

>Translated_159_residues
MTTFNGKINGNGLKIGIAVARFNAFVTQQLLTGAQESLVQHGVNETDIDVAWVPGAFEIPLTVQKMITTKRYDGVIALGA
VIRGATAHFDYVCSGVTTGIATVSLATDTPIMFGVLTTDTIEQAMDRAGFKSGNKGADCAVSLLETLDVQRAILKADLA
>Mature_158_residues
TTFNGKINGNGLKIGIAVARFNAFVTQQLLTGAQESLVQHGVNETDIDVAWVPGAFEIPLTVQKMITTKRYDGVIALGAV
IRGATAHFDYVCSGVTTGIATVSLATDTPIMFGVLTTDTIEQAMDRAGFKSGNKGADCAVSLLETLDVQRAILKADLA

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=141, Percent_Identity=50.354609929078, Blast_Score=150, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6324429, Length=146, Percent_Identity=32.8767123287671, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_LACPL (Q88X16)

Other databases:

- EMBL:   AL935256
- RefSeq:   NP_785052.1
- ProteinModelPortal:   Q88X16
- SMR:   Q88X16
- GeneID:   1062420
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1438
- NMPDR:   fig|220668.1.peg.1186
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- ProtClustDB:   CLSK2520205
- BioCyc:   LPLA220668:LP_1438-MONOMER
- BRENDA:   2.5.1.9
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16748; Mature: 16617

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFNGKINGNGLKIGIAVARFNAFVTQQLLTGAQESLVQHGVNETDIDVAWVPGAFEIP
CCCCCCEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEECC
LTVQKMITTKRYDGVIALGAVIRGATAHFDYVCSGVTTGIATVSLATDTPIMFGVLTTDT
HHHHHHHHHHHCCCEEEEHHHHHCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECHHH
IEQAMDRAGFKSGNKGADCAVSLLETLDVQRAILKADLA
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTFNGKINGNGLKIGIAVARFNAFVTQQLLTGAQESLVQHGVNETDIDVAWVPGAFEIP
CCCCCEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEECC
LTVQKMITTKRYDGVIALGAVIRGATAHFDYVCSGVTTGIATVSLATDTPIMFGVLTTDT
HHHHHHHHHHHCCCEEEEHHHHHCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECHHH
IEQAMDRAGFKSGNKGADCAVSLLETLDVQRAILKADLA
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566