Click here to switch to the map view.

The map label for this gene is pbp2A [H]

Identifier: 28378142

GI number: 28378142

Start: 1292852

End: 1294981

Strand: Direct

Name: pbp2A [H]

Synonym: lp_1413

Alternate gene names: 28378142

Gene position: 1292852-1294981 (Clockwise)

Preceding gene: 28378141

Following gene: 28378143

Centisome position: 39.08

GC content: 44.6

Gene sequence:

>2130_bases
ATGCAAAAAAATGGTTTTTGGGCCACGATCAAAGACGGCTTACGAGTCATTGGCAATTGGTTAGCACCCTATTGGCAGCG
ATTCGCTGCCGTCGTCGGCTACCAATGGCACCGGCGACAAATCACACGGTGGCTAATTGTACTCGTCTTGACCGTCATCT
TAATTGGGAGTGCCTATTTAACTTATGAAGCCAAGACGGCTAAAGTTGGTAATTTACAGGCGGAGCTAGAAAAGACAACT
GAAATCTATGATAAGGACAATAAAAAGGCCGGTTCACTCTATTCACAAAAGGGAACCTATGTCCATTTGAGTAGTATTTC
AAAAAACTTACAGAACGCGGTAATTTCGACTGAAGACCGTAATTTCTATAAAGAACATGGTTTCTCAGTCAAAGGAATCG
GGCGGGCATTTGTCCTGCTCGTTATCAATAAGATACTTGGCCGTGACTACATTAGTGGTGGTGGGAGTACGTTGACGCAA
CAATTGGTCAAAAACGCCTACCTAACACAACAACAGACCTTCTCACGTAAGTTTCGGGAAATCTTTTTGGCGATTGAAAC
TGAAAATGTCTATTCTAAGGGCCAAATTTTAGCGATGTATTTGAATAACGCTTATTTCGGCCACGGTGTCTGGGGAGCTG
AGGATGCGTCTGAACGATATTTTGGCGTACACGCGTCAGAGTTGTCTGTTGACCAGGCGGCGACCTTGGCCGGAATGCTG
AGCTCGCCCAGTGGTTATGATCCAATCAATCATCCGAAAGCGTCTACGGCTCGTCGAAACGTCGTCTTGAATAACATGGT
CGCTAATAATAAGCTTTCCAAAAGTGAGTATAAGCTTTATTCGCAAAAGGCGATGACGCTGACGAATAACTACCATTATG
AAAGTGGTTATAATTATCCATATTATTTCGATGCGGTGATTGATGAAGCAATTAATAAGTACGGGCTAACGGAATCTGAC
ATTATGAATCGGGGCTATAAGATTTATACGTCGCTTGATCAAGATGATCAGACACAGATGCAAGATTCTTTTAAGGATAG
TACGTTGTTCCCGGCCAACGCGGACGATGGTACGAAGGTCCAAGGGGCCTCAATTGCGGTTGATCCGTCGACGGGTGGTG
TGTTAGCCGTAGTCGGTGGTCGGGGCAAACACGTCTTCCGGGGCTTTAACCGTGCGACTCAGATTAAGCGGCAGCCGGGT
TCGACGATCAAACCGTTGGCAGTGTATACACCAGCACTACAAAATGGTTATACGTATGATTCGAACCTTTCTAATAAGAA
GCAAACTTTTGGGGCCAATAAATATGCGCCCAAAAACTATGATAATGTTTATAGTAAATCGGTCCCAATGTATACCGCGT
TATCGCAAAGTATGAATATTCCGGCGGTTTGGTTATTGAATAAGATTGGTGTGAATAAAGGGTATCAATCGGTGAAAAAG
TTTGGCTTACCAGTTACCAAGTCGGATGATAACCTGGCACTTGCCTTAGGTGGTTTGACGACGGGGGTATCACCCGCTCA
AATGACCAGTGCCTATACAGCGTTTGCGAATGGTGGTAAGAAAACGACGGCCCACTTTATTACGAAAATTGTCGATGCTA
GTGGCAATGTCGTTGTCGATAATACGAAGACTAAGACGAAGAAAATTATGTCCGCCAGCGTTGCTAAAGAAATGACCAGT
ATGATGCTTGGCACGTACAATAGTGGGACCGGGGCTGCGGCCAAGCCGTATGGATACTCGGTTGCTGGTAAGACTGGGAG
TACGCAGGCTGATTACAGTACGGGTTCGGGAACGAAGGATCAGTGGATGATTGCTTATACCCCGGACATTGTGGTCACGA
CCTGGATCGGATTTGATACGACGAACAGTACTCATTATTTGAAGAGTTTATCTGAAAATCAACTTTCCTCGTTATTCAAG
AATGAATTACAAAATATTTTGCCGAATACGAATAATACTAGTTTTGGTACGAAGGATGCTGCGACGCTGGCAACCGAATC
TAATAGTAGTGATTCGGATAGTTCTGATAGTGGATCATCCGTATGGGAAAACGTTGAGAAGGGCGCTAATGCAGTTAAGA
ACAAGGCTAAAGACTGGTTCTCCAAAGCCAAAAGTTTCCTTGGCAACTAG

Upstream 100 bases:

>100_bases
GGTCAATTACTGAGCATCGGAAAATTTATGATACAATTGTAAAAGCTAACAAGTAACGCGGACGTTGTGACAGGTGCGTC
GGCTGAACAAGGGGAAGATT

Downstream 100 bases:

>100_bases
TAAAGCTAGCTTCTGAACGTACTAATGCTATTCAAGTTCCGTTAAAAATGCTAAAATAATGACGACACATACTTCGAGGA
GGAACTCACGATGGCCGTAA

Product: penicillin binding protein 2A

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 709; Mature: 709

Protein sequence:

>709_residues
MQKNGFWATIKDGLRVIGNWLAPYWQRFAAVVGYQWHRRQITRWLIVLVLTVILIGSAYLTYEAKTAKVGNLQAELEKTT
EIYDKDNKKAGSLYSQKGTYVHLSSISKNLQNAVISTEDRNFYKEHGFSVKGIGRAFVLLVINKILGRDYISGGGSTLTQ
QLVKNAYLTQQQTFSRKFREIFLAIETENVYSKGQILAMYLNNAYFGHGVWGAEDASERYFGVHASELSVDQAATLAGML
SSPSGYDPINHPKASTARRNVVLNNMVANNKLSKSEYKLYSQKAMTLTNNYHYESGYNYPYYFDAVIDEAINKYGLTESD
IMNRGYKIYTSLDQDDQTQMQDSFKDSTLFPANADDGTKVQGASIAVDPSTGGVLAVVGGRGKHVFRGFNRATQIKRQPG
STIKPLAVYTPALQNGYTYDSNLSNKKQTFGANKYAPKNYDNVYSKSVPMYTALSQSMNIPAVWLLNKIGVNKGYQSVKK
FGLPVTKSDDNLALALGGLTTGVSPAQMTSAYTAFANGGKKTTAHFITKIVDASGNVVVDNTKTKTKKIMSASVAKEMTS
MMLGTYNSGTGAAAKPYGYSVAGKTGSTQADYSTGSGTKDQWMIAYTPDIVVTTWIGFDTTNSTHYLKSLSENQLSSLFK
NELQNILPNTNNTSFGTKDAATLATESNSSDSDSSDSGSSVWENVEKGANAVKNKAKDWFSKAKSFLGN

Sequences:

>Translated_709_residues
MQKNGFWATIKDGLRVIGNWLAPYWQRFAAVVGYQWHRRQITRWLIVLVLTVILIGSAYLTYEAKTAKVGNLQAELEKTT
EIYDKDNKKAGSLYSQKGTYVHLSSISKNLQNAVISTEDRNFYKEHGFSVKGIGRAFVLLVINKILGRDYISGGGSTLTQ
QLVKNAYLTQQQTFSRKFREIFLAIETENVYSKGQILAMYLNNAYFGHGVWGAEDASERYFGVHASELSVDQAATLAGML
SSPSGYDPINHPKASTARRNVVLNNMVANNKLSKSEYKLYSQKAMTLTNNYHYESGYNYPYYFDAVIDEAINKYGLTESD
IMNRGYKIYTSLDQDDQTQMQDSFKDSTLFPANADDGTKVQGASIAVDPSTGGVLAVVGGRGKHVFRGFNRATQIKRQPG
STIKPLAVYTPALQNGYTYDSNLSNKKQTFGANKYAPKNYDNVYSKSVPMYTALSQSMNIPAVWLLNKIGVNKGYQSVKK
FGLPVTKSDDNLALALGGLTTGVSPAQMTSAYTAFANGGKKTTAHFITKIVDASGNVVVDNTKTKTKKIMSASVAKEMTS
MMLGTYNSGTGAAAKPYGYSVAGKTGSTQADYSTGSGTKDQWMIAYTPDIVVTTWIGFDTTNSTHYLKSLSENQLSSLFK
NELQNILPNTNNTSFGTKDAATLATESNSSDSDSSDSGSSVWENVEKGANAVKNKAKDWFSKAKSFLGN
>Mature_709_residues
MQKNGFWATIKDGLRVIGNWLAPYWQRFAAVVGYQWHRRQITRWLIVLVLTVILIGSAYLTYEAKTAKVGNLQAELEKTT
EIYDKDNKKAGSLYSQKGTYVHLSSISKNLQNAVISTEDRNFYKEHGFSVKGIGRAFVLLVINKILGRDYISGGGSTLTQ
QLVKNAYLTQQQTFSRKFREIFLAIETENVYSKGQILAMYLNNAYFGHGVWGAEDASERYFGVHASELSVDQAATLAGML
SSPSGYDPINHPKASTARRNVVLNNMVANNKLSKSEYKLYSQKAMTLTNNYHYESGYNYPYYFDAVIDEAINKYGLTESD
IMNRGYKIYTSLDQDDQTQMQDSFKDSTLFPANADDGTKVQGASIAVDPSTGGVLAVVGGRGKHVFRGFNRATQIKRQPG
STIKPLAVYTPALQNGYTYDSNLSNKKQTFGANKYAPKNYDNVYSKSVPMYTALSQSMNIPAVWLLNKIGVNKGYQSVKK
FGLPVTKSDDNLALALGGLTTGVSPAQMTSAYTAFANGGKKTTAHFITKIVDASGNVVVDNTKTKTKKIMSASVAKEMTS
MMLGTYNSGTGAAAKPYGYSVAGKTGSTQADYSTGSGTKDQWMIAYTPDIVVTTWIGFDTTNSTHYLKSLSENQLSSLFK
NELQNILPNTNNTSFGTKDAATLATESNSSDSDSSDSGSSVWENVEKGANAVKNKAKDWFSKAKSFLGN

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=544, Percent_Identity=29.5955882352941, Blast_Score=188, Evalue=9e-49,
Organism=Escherichia coli, GI87082258, Length=317, Percent_Identity=29.9684542586751, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1788867, Length=530, Percent_Identity=27.5471698113208, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1789601, Length=161, Percent_Identity=39.1304347826087, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 77774; Mature: 77774

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKNGFWATIKDGLRVIGNWLAPYWQRFAAVVGYQWHRRQITRWLIVLVLTVILIGSAYL
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHE
TYEAKTAKVGNLQAELEKTTEIYDKDNKKAGSLYSQKGTYVHLSSISKNLQNAVISTEDR
EEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHCCCCCEEEHHHHHHHHHHHEECCCCC
NFYKEHGFSVKGIGRAFVLLVINKILGRDYISGGGSTLTQQLVKNAYLTQQQTFSRKFRE
HHHHHCCCCEEHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IFLAIETENVYSKGQILAMYLNNAYFGHGVWGAEDASERYFGVHASELSVDQAATLAGML
HEEEEECCCCCCCCCEEEEEECCCEECCCCCCCCCCCCCEEECCHHHCCHHHHHHHHHHH
SSPSGYDPINHPKASTARRNVVLNNMVANNKLSKSEYKLYSQKAMTLTNNYHYESGYNYP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEECCEEECCCCCCC
YYFDAVIDEAINKYGLTESDIMNRGYKIYTSLDQDDQTQMQDSFKDSTLFPANADDGTKV
EEHHHHHHHHHHHCCCCHHHHHHCCEEEEEECCCCHHHHHHHHCCCCEECCCCCCCCCEE
QGASIAVDPSTGGVLAVVGGRGKHVFRGFNRATQIKRQPGSTIKPLAVYTPALQNGYTYD
CCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHCCCCCC
SNLSNKKQTFGANKYAPKNYDNVYSKSVPMYTALSQSMNIPAVWLLNKIGVNKGYQSVKK
CCCCCCHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHCCCCCCEEHHHHHCCCCCHHHHHH
FGLPVTKSDDNLALALGGLTTGVSPAQMTSAYTAFANGGKKTTAHFITKIVDASGNVVVD
HCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEC
NTKTKTKKIMSASVAKEMTSMMLGTYNSGTGAAAKPYGYSVAGKTGSTQADYSTGSGTKD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCC
QWMIAYTPDIVVTTWIGFDTTNSTHYLKSLSENQLSSLFKNELQNILPNTNNTSFGTKDA
CEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
ATLATESNSSDSDSSDSGSSVWENVEKGANAVKNKAKDWFSKAKSFLGN
HEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQKNGFWATIKDGLRVIGNWLAPYWQRFAAVVGYQWHRRQITRWLIVLVLTVILIGSAYL
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHE
TYEAKTAKVGNLQAELEKTTEIYDKDNKKAGSLYSQKGTYVHLSSISKNLQNAVISTEDR
EEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHCCCCCEEEHHHHHHHHHHHEECCCCC
NFYKEHGFSVKGIGRAFVLLVINKILGRDYISGGGSTLTQQLVKNAYLTQQQTFSRKFRE
HHHHHCCCCEEHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IFLAIETENVYSKGQILAMYLNNAYFGHGVWGAEDASERYFGVHASELSVDQAATLAGML
HEEEEECCCCCCCCCEEEEEECCCEECCCCCCCCCCCCCEEECCHHHCCHHHHHHHHHHH
SSPSGYDPINHPKASTARRNVVLNNMVANNKLSKSEYKLYSQKAMTLTNNYHYESGYNYP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEECCEEECCCCCCC
YYFDAVIDEAINKYGLTESDIMNRGYKIYTSLDQDDQTQMQDSFKDSTLFPANADDGTKV
EEHHHHHHHHHHHCCCCHHHHHHCCEEEEEECCCCHHHHHHHHCCCCEECCCCCCCCCEE
QGASIAVDPSTGGVLAVVGGRGKHVFRGFNRATQIKRQPGSTIKPLAVYTPALQNGYTYD
CCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHCCCCCC
SNLSNKKQTFGANKYAPKNYDNVYSKSVPMYTALSQSMNIPAVWLLNKIGVNKGYQSVKK
CCCCCCHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHCCCCCCEEHHHHHCCCCCHHHHHH
FGLPVTKSDDNLALALGGLTTGVSPAQMTSAYTAFANGGKKTTAHFITKIVDASGNVVVD
HCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEC
NTKTKTKKIMSASVAKEMTSMMLGTYNSGTGAAAKPYGYSVAGKTGSTQADYSTGSGTKD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCC
QWMIAYTPDIVVTTWIGFDTTNSTHYLKSLSENQLSSLFKNELQNILPNTNNTSFGTKDA
CEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
ATLATESNSSDSDSSDSGSSVWENVEKGANAVKNKAKDWFSKAKSFLGN
HEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]