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The map label for this gene is metK

Identifier: 28378057

GI number: 28378057

Start: 1189935

End: 1191122

Strand: Direct

Name: metK

Synonym: lp_1301

Alternate gene names: 28378057

Gene position: 1189935-1191122 (Clockwise)

Preceding gene: 28378055

Following gene: 28378058

Centisome position: 35.97

GC content: 47.47

Gene sequence:

>1188_bases
GTGAGTGAAAGACACTTATTTACATCTGAATCTGTCTCTGAAGGTCATCCTGATAAGATTGCTGACCAAATCAGTGACGC
TATCCTGGACGCAATGTTAGCCCAGGATCCACAAGCACGGGTCGCTGTTGAAACTTCAGTGACGACTGGATTAGTGCTGG
TCTTCGGTGAAGTTTCGACCAAGGCCTACGTTGATATTCAAAAGGTCGTGCGTGACACGATCAAGTCGATTGGCTATGTT
GATGGTCAATACGGCTTTGACGGTGACAACTGTGCCGTCCTTGTCTCATTGGATGAACAATCACCAGATATCGCGCAAGG
GGTCGATGATTCCTTGGAGACGCGTTCTGGCGATGCTGACCCATTAGATCAAATCGGTGCTGGTGATCAAGGGATGATGT
TTGGTTATGCCATCAACGAAACCCCTGAATTAATGCCACTGCCAATCGCATTGAGCCATCGGTTGATGCGTAAAATTGCA
GCGCTACGTAAGGATGGTACCATTAAGTGGTTACGTCCAGATGCTAAGGCCCAAGTCACGGTCGAATATGACGAAGACAA
CCAACCTAAGCGAATCGACACGGTGGTCTTGTCTACTCAGCATGATCCTGATGTTGATTTGGACACAATCCGGCAAACGG
TCATTGATCAAGTCATCAAGGCTGTGTTACCAGCTGATTTATTAGATGACCAAACTAAGTATCTTGTGAACCCAACTGGT
CGTTTTGTTATTGGTGGTCCTCAAGGGGATGCCGGTTTGACTGGTCGGAAAGTCATCGTGGATACGTATGGTGGTTTTGC
CCATCATGGTGGTGGTGCCTTTTCTGGTAAGGACGCGACCAAGGTTGATCGTTCTGCAAGTTACGCTGCTCGTTACATTG
CCAAAAACGTTGTGGCTGCCGGTTTAGCTGATCAGGTCGAAGTGCAATTGGCATATGCCATCGGGGTTGCCGAACCAGTT
TCGATTGCAGTGGATACTGCCGGGACCGGGAAGGTTAGTGATGAAGCGTTGATTAACGCCATTCGCGAAAACTTTGATTT
ACGCCCAGCTGGGATTATCAAAATGCTCGATTTGCAACGGCCAATTTATCGGCAAACTGCGGCATACGGCCACTTTGGTC
GGACCGATATTGATTTGCCATGGGAACATACTGATAAAGTGGATGCTTTAAAAGCAGTATTTAAATAA

Upstream 100 bases:

>100_bases
AGTTATGAGCCGTTCCCGAAAGGATAAGTTTAGCTTGTTCGACGAAGAATAAGTGATGCTTAGATGCCTTGTAACCGAAA
TTATCAAGGGGGAATTTATT

Downstream 100 bases:

>100_bases
TGCGGTAACGTTGATTCGTTACGGTCTGTAGGTACTCGACGCTAAAAGCGACCTTTTTTCAAGGTCGCTTTTTTTTGCGG
GCTATTTTAGATGCACGGAG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MSERHLFTSESVSEGHPDKIADQISDAILDAMLAQDPQARVAVETSVTTGLVLVFGEVSTKAYVDIQKVVRDTIKSIGYV
DGQYGFDGDNCAVLVSLDEQSPDIAQGVDDSLETRSGDADPLDQIGAGDQGMMFGYAINETPELMPLPIALSHRLMRKIA
ALRKDGTIKWLRPDAKAQVTVEYDEDNQPKRIDTVVLSTQHDPDVDLDTIRQTVIDQVIKAVLPADLLDDQTKYLVNPTG
RFVIGGPQGDAGLTGRKVIVDTYGGFAHHGGGAFSGKDATKVDRSASYAARYIAKNVVAAGLADQVEVQLAYAIGVAEPV
SIAVDTAGTGKVSDEALINAIRENFDLRPAGIIKMLDLQRPIYRQTAAYGHFGRTDIDLPWEHTDKVDALKAVFK

Sequences:

>Translated_395_residues
MSERHLFTSESVSEGHPDKIADQISDAILDAMLAQDPQARVAVETSVTTGLVLVFGEVSTKAYVDIQKVVRDTIKSIGYV
DGQYGFDGDNCAVLVSLDEQSPDIAQGVDDSLETRSGDADPLDQIGAGDQGMMFGYAINETPELMPLPIALSHRLMRKIA
ALRKDGTIKWLRPDAKAQVTVEYDEDNQPKRIDTVVLSTQHDPDVDLDTIRQTVIDQVIKAVLPADLLDDQTKYLVNPTG
RFVIGGPQGDAGLTGRKVIVDTYGGFAHHGGGAFSGKDATKVDRSASYAARYIAKNVVAAGLADQVEVQLAYAIGVAEPV
SIAVDTAGTGKVSDEALINAIRENFDLRPAGIIKMLDLQRPIYRQTAAYGHFGRTDIDLPWEHTDKVDALKAVFK
>Mature_394_residues
SERHLFTSESVSEGHPDKIADQISDAILDAMLAQDPQARVAVETSVTTGLVLVFGEVSTKAYVDIQKVVRDTIKSIGYVD
GQYGFDGDNCAVLVSLDEQSPDIAQGVDDSLETRSGDADPLDQIGAGDQGMMFGYAINETPELMPLPIALSHRLMRKIAA
LRKDGTIKWLRPDAKAQVTVEYDEDNQPKRIDTVVLSTQHDPDVDLDTIRQTVIDQVIKAVLPADLLDDQTKYLVNPTGR
FVIGGPQGDAGLTGRKVIVDTYGGFAHHGGGAFSGKDATKVDRSASYAARYIAKNVVAAGLADQVEVQLAYAIGVAEPVS
IAVDTAGTGKVSDEALINAIRENFDLRPAGIIKMLDLQRPIYRQTAAYGHFGRTDIDLPWEHTDKVDALKAVFK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=58.6842105263158, Blast_Score=437, Evalue=1e-123,
Organism=Homo sapiens, GI5174529, Length=380, Percent_Identity=58.9473684210526, Blast_Score=433, Evalue=1e-121,
Organism=Escherichia coli, GI1789311, Length=388, Percent_Identity=63.1443298969072, Blast_Score=506, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17538498, Length=383, Percent_Identity=57.7023498694517, Blast_Score=438, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17538494, Length=383, Percent_Identity=57.1801566579634, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17551082, Length=385, Percent_Identity=55.5844155844156, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI25145633, Length=388, Percent_Identity=55.9278350515464, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32565905, Length=383, Percent_Identity=51.6971279373368, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32565911, Length=336, Percent_Identity=55.3571428571429, Blast_Score=366, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25145635, Length=340, Percent_Identity=53.5294117647059, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI32565903, Length=308, Percent_Identity=55.1948051948052, Blast_Score=331, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI32565909, Length=86, Percent_Identity=59.3023255813954, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=39, Percent_Identity=79.4871794871795, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=385, Percent_Identity=58.7012987012987, Blast_Score=449, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6320710, Length=390, Percent_Identity=57.1794871794872, Blast_Score=443, Evalue=1e-125,
Organism=Drosophila melanogaster, GI45552159, Length=381, Percent_Identity=56.4304461942257, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580529, Length=381, Percent_Identity=56.4304461942257, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580537, Length=381, Percent_Identity=56.4304461942257, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580533, Length=381, Percent_Identity=56.4304461942257, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580535, Length=381, Percent_Identity=56.4304461942257, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580531, Length=381, Percent_Identity=56.4304461942257, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580527, Length=381, Percent_Identity=55.6430446194226, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580525, Length=381, Percent_Identity=55.6430446194226, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580541, Length=383, Percent_Identity=53.2637075718016, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580539, Length=125, Percent_Identity=55.2, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_LACPL (Q88XB8)

Other databases:

- EMBL:   AL935255
- RefSeq:   NP_784949.1
- ProteinModelPortal:   Q88XB8
- SMR:   Q88XB8
- GeneID:   1062756
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1301
- NMPDR:   fig|220668.1.peg.1083
- HOGENOM:   HBG443662
- OMA:   QDEHARV
- ProtClustDB:   PRK05250
- BioCyc:   LPLA220668:LP_1301-MONOMER
- BRENDA:   2.5.1.6
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42679; Mature: 42548

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERHLFTSESVSEGHPDKIADQISDAILDAMLAQDPQARVAVETSVTTGLVLVFGEVST
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCC
KAYVDIQKVVRDTIKSIGYVDGQYGFDGDNCAVLVSLDEQSPDIAQGVDDSLETRSGDAD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCHHHHHCCCCCC
PLDQIGAGDQGMMFGYAINETPELMPLPIALSHRLMRKIAALRKDGTIKWLRPDAKAQVT
CHHHHCCCCCCEEEEEEECCCCCCEECCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEE
VEYDEDNQPKRIDTVVLSTQHDPDVDLDTIRQTVIDQVIKAVLPADLLDDQTKYLVNPTG
EEECCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCCEEECCCC
RFVIGGPQGDAGLTGRKVIVDTYGGFAHHGGGAFSGKDATKVDRSASYAARYIAKNVVAA
CEEEECCCCCCCCCCCEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GLADQVEVQLAYAIGVAEPVSIAVDTAGTGKVSDEALINAIRENFDLRPAGIIKMLDLQR
CCCHHHEEEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCC
PIYRQTAAYGHFGRTDIDLPWEHTDKVDALKAVFK
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
SERHLFTSESVSEGHPDKIADQISDAILDAMLAQDPQARVAVETSVTTGLVLVFGEVST
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCC
KAYVDIQKVVRDTIKSIGYVDGQYGFDGDNCAVLVSLDEQSPDIAQGVDDSLETRSGDAD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCHHHHHCCCCCC
PLDQIGAGDQGMMFGYAINETPELMPLPIALSHRLMRKIAALRKDGTIKWLRPDAKAQVT
CHHHHCCCCCCEEEEEEECCCCCCEECCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEE
VEYDEDNQPKRIDTVVLSTQHDPDVDLDTIRQTVIDQVIKAVLPADLLDDQTKYLVNPTG
EEECCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCCEEECCCC
RFVIGGPQGDAGLTGRKVIVDTYGGFAHHGGGAFSGKDATKVDRSASYAARYIAKNVVAA
CEEEECCCCCCCCCCCEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GLADQVEVQLAYAIGVAEPVSIAVDTAGTGKVSDEALINAIRENFDLRPAGIIKMLDLQR
CCCHHHEEEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCC
PIYRQTAAYGHFGRTDIDLPWEHTDKVDALKAVFK
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566