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The map label for this gene is bmr3 [H]

Identifier: 28378058

GI number: 28378058

Start: 1191259

End: 1192761

Strand: Direct

Name: bmr3 [H]

Synonym: lp_1302

Alternate gene names: 28378058

Gene position: 1191259-1192761 (Clockwise)

Preceding gene: 28378057

Following gene: 28378059

Centisome position: 36.01

GC content: 46.44

Gene sequence:

>1503_bases
ATGCAACAGCGTAAAACTAACGTAATGGCGGTTACGGTTGCCATTTATGTCGCGACGTTTATGAGTGCGATTGAAGGGAC
AATCGTGTCGACCGCCTTGCCAACGATTGTTGGTGATTTACACGGGGTTTCATTGATGAATTGGGTTTTCTCAATTTATT
TATTGACTAATGCGATGATGACGCCAATTTATGGAAAGCTGACTGACTTGGTCGGCCGAAAACCAGTGATGCAAGCCGGA
CTAATTATTTTTATTATCGGGTCTATGATGAGCGGTTTGTCTAATTCGATGCCGGTGCTAATTTTTTGGCGGGCCATTCA
GGGGATCGGTGCCGGGGCGCTAATGCCAGTCTCAATGACAATTATCGCAGATATTTATTCATTCGAACGCCGGGCCAAAG
TGCTAGGCTTCAATAGTTCAGCGTGGGGCATTGCTTCAGTGCTGGCACCGCTGATTGGTGGACTAATTGTCGATAAGCTC
AGCTGGCATTGGATTTTCTTCATTAATGTGCCAGTTGGGTTGATCACCCTTTTCCTATTTCAATTTTATTTACGAGAACC
CAAACGTCACAATAACGTGAAAATCGACTACTTGGGTAGCTTTTGGCTGATGCTCTTCTTGCTGTGCTTGATGCTAAGCT
TCCAACTATTGGGAAACGCCACTATCAGTTGGGTGGCCGTTGGGACTGCTTGGATCATTAGTGCGCTCAGTTTGTGGCTA
TTTATTCGTCGTGAAAGTCGCACGGATGAACCAGTGATTTCGATGGATTTATTCAAAAACCACACGTTTATTATTCAGAA
TGCCGTGGCGGCCTTAGTCAGTGGTTTCCTAATGGCCGTTGAAGTTTATATTCCAACTTGGACTCAGGGGATACTGGGTG
TACCGGCTTCTTTGGCGGGATTCGCAGTGACACCGAGCTCGTTAATGTGGATCGTCGGGTCATTCGTGACGGGGCGGTTA
TTGGCAAAGTGGGCGCCGCGACGGATTTTGTATCTGAGTTTGGCTTTCATTCTCGTGACAAGCATTTGCTTAGCGTGGTT
ACCAGTTAATACTGCTTTCCCATGGTTCTTCCTGATCACAGCGATTGGTGGCGTGGGCTTTGGAATTACAATTACAGCAA
CGACCGTCACGTCGCAACACCTTGTGCCAGCTGAGAACGTGGGCGTCGCAACGTCCTTCAATACGTTGAGTCGAACGATT
GGCCAAACGTTGATGATTTCAATCTTTGGAATTGCGTTGAACGTTGGGATGAACCAAGGTATTCAGCACCATGCAGGAAC
GAACATGGCGATGATGAACAAGTTGATCAATCCACAGACAGCAACGCAATTACCGTCTCATTTGTTGCCGACACTTCATC
GTATCTTATATACGGGCTTACATTGGGTCTACCTGATTGGCCTTGGTTTGGTTATTCTAGCCTTTATTGTGAATAGTTTG
GATCGCGGACCACAAAAAACGGCAGCACAACACGCGGCTGATTTAAACCAAAATCAGGATTAG

Upstream 100 bases:

>100_bases
CGACGCTAAAAGCGACCTTTTTTCAAGGTCGCTTTTTTTTGCGGGCTATTTTAGATGCACGGAGTTTTAAAATGATTGAT
AGAGAAGGAGAATTACTAAG

Downstream 100 bases:

>100_bases
TCGGCTTAGTATCAACAAATTAATTGTCAAAGCGACTCATAATTTGTCGTTTATTTGATATTGACGTAAAAAAAATCTGA
AAAATTTCCAATAAAATTTC

Product: multidrug transport protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MQQRKTNVMAVTVAIYVATFMSAIEGTIVSTALPTIVGDLHGVSLMNWVFSIYLLTNAMMTPIYGKLTDLVGRKPVMQAG
LIIFIIGSMMSGLSNSMPVLIFWRAIQGIGAGALMPVSMTIIADIYSFERRAKVLGFNSSAWGIASVLAPLIGGLIVDKL
SWHWIFFINVPVGLITLFLFQFYLREPKRHNNVKIDYLGSFWLMLFLLCLMLSFQLLGNATISWVAVGTAWIISALSLWL
FIRRESRTDEPVISMDLFKNHTFIIQNAVAALVSGFLMAVEVYIPTWTQGILGVPASLAGFAVTPSSLMWIVGSFVTGRL
LAKWAPRRILYLSLAFILVTSICLAWLPVNTAFPWFFLITAIGGVGFGITITATTVTSQHLVPAENVGVATSFNTLSRTI
GQTLMISIFGIALNVGMNQGIQHHAGTNMAMMNKLINPQTATQLPSHLLPTLHRILYTGLHWVYLIGLGLVILAFIVNSL
DRGPQKTAAQHAADLNQNQD

Sequences:

>Translated_500_residues
MQQRKTNVMAVTVAIYVATFMSAIEGTIVSTALPTIVGDLHGVSLMNWVFSIYLLTNAMMTPIYGKLTDLVGRKPVMQAG
LIIFIIGSMMSGLSNSMPVLIFWRAIQGIGAGALMPVSMTIIADIYSFERRAKVLGFNSSAWGIASVLAPLIGGLIVDKL
SWHWIFFINVPVGLITLFLFQFYLREPKRHNNVKIDYLGSFWLMLFLLCLMLSFQLLGNATISWVAVGTAWIISALSLWL
FIRRESRTDEPVISMDLFKNHTFIIQNAVAALVSGFLMAVEVYIPTWTQGILGVPASLAGFAVTPSSLMWIVGSFVTGRL
LAKWAPRRILYLSLAFILVTSICLAWLPVNTAFPWFFLITAIGGVGFGITITATTVTSQHLVPAENVGVATSFNTLSRTI
GQTLMISIFGIALNVGMNQGIQHHAGTNMAMMNKLINPQTATQLPSHLLPTLHRILYTGLHWVYLIGLGLVILAFIVNSL
DRGPQKTAAQHAADLNQNQD
>Mature_500_residues
MQQRKTNVMAVTVAIYVATFMSAIEGTIVSTALPTIVGDLHGVSLMNWVFSIYLLTNAMMTPIYGKLTDLVGRKPVMQAG
LIIFIIGSMMSGLSNSMPVLIFWRAIQGIGAGALMPVSMTIIADIYSFERRAKVLGFNSSAWGIASVLAPLIGGLIVDKL
SWHWIFFINVPVGLITLFLFQFYLREPKRHNNVKIDYLGSFWLMLFLLCLMLSFQLLGNATISWVAVGTAWIISALSLWL
FIRRESRTDEPVISMDLFKNHTFIIQNAVAALVSGFLMAVEVYIPTWTQGILGVPASLAGFAVTPSSLMWIVGSFVTGRL
LAKWAPRRILYLSLAFILVTSICLAWLPVNTAFPWFFLITAIGGVGFGITITATTVTSQHLVPAENVGVATSFNTLSRTI
GQTLMISIFGIALNVGMNQGIQHHAGTNMAMMNKLINPQTATQLPSHLLPTLHRILYTGLHWVYLIGLGLVILAFIVNSL
DRGPQKTAAQHAADLNQNQD

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=457, Percent_Identity=28.2275711159737, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1790195, Length=319, Percent_Identity=32.2884012539185, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788710, Length=415, Percent_Identity=29.8795180722892, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87081983, Length=423, Percent_Identity=26.7139479905437, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1788392, Length=428, Percent_Identity=26.8691588785047, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790146, Length=299, Percent_Identity=28.7625418060201, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081738, Length=129, Percent_Identity=34.8837209302326, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1788509, Length=148, Percent_Identity=31.7567567567568, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319770, Length=399, Percent_Identity=28.3208020050125, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323735, Length=327, Percent_Identity=25.3822629969419, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321663, Length=146, Percent_Identity=33.5616438356164, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322958, Length=441, Percent_Identity=24.0362811791383, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6325455, Length=166, Percent_Identity=30.1204819277108, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324264, Length=147, Percent_Identity=29.2517006802721, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54808; Mature: 54808

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure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CCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]