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The map label for this gene is dacA2 [H]

Identifier: 28378040

GI number: 28378040

Start: 1163084

End: 1164322

Strand: Direct

Name: dacA2 [H]

Synonym: lp_1278

Alternate gene names: 28378040

Gene position: 1163084-1164322 (Clockwise)

Preceding gene: 28378039

Following gene: 28378042

Centisome position: 35.16

GC content: 48.91

Gene sequence:

>1239_bases
ATGTTAAAGTTAATCAAACAACGGTTGGTATGGGGACTAGTGTTGACAGCGACAGTCAGCGGGGTGTTGGGCTGCAATGT
AGCGGCACACGCGACCAGTAGTAAGGCGGATGAGGTCAAAGCCGCCTACGTGATGGATGCCAAGTCTGGTCAGGTCGTTT
ATGCGCAGAATGCCGATCAGAAATTACCAATTGCGTCCTTGAGTAAGCTCATGACGCTGTATTTAGTTGAACGGGCCATC
GAGCATGGTCAAATCAAGTGGACGGATGCAGTCCCAATTCGCAAAAATGTCCGCAAGATGGCAACCAGTGCAACTTTATC
CGTAATGCCGATGCCTGCCAAGGAGACGTTTACGGTTAGGGAGTTATTCGCAGCTACCTTGGTAGGGTCTTCGAATAGTG
GTGCCATTGCGCTGGGGGAGTATGTTGCTGGCAGTAATGATAAGTTCATTAAATTAATGAATCAACAAGCGGCCAAATGG
CATTTGGATGCGAGTTTTATTAGCGCTTCGGGACTTGATAATACTGATTTAAAAAATTACGATTTAAAAATCAAGGGAAC
CAGCGATCAGGACCAAAATCTGGTGTCAGCAAGGGCTGTGACGACCATTGCGCAACATTTGATTGATGCTTACCCCGATG
TTATTCAAGTTGCTAATCAACGTTCAGTCAAAATTCATAAGTATACGGTCCCAACTTCGGTGGAGATCCTGAAGGGCGCC
GAGTACTATGATCCCCGCGTGCCAGTCGATGGACTGAAGACCGGTTATACGAATCAGGCCGGTGCTTGCTTAGTCGCTAC
ATACCGGCAGAACGGCCACCGGATGATTGCAACAACACTTGGCGGTGCGTGGCAGTTTACGGCGAACAACAACTTGCGGC
TGATGCTCAAAACGACCGAGCAGTATAAGCAGGTTGTGCCTAAGAGCATCAATTATCAGATTCCGGGGACGCAGACCAAA
GTGCGTCTGGTGACGAAGTACAATTCAAAACTGTGGGTGAATAGTAAGCAGCCAATGACGCAGACCAAAATTGCTTTGTG
GCCATTACATCGGACAGCGCCAAATTATCTGGCTGCTAATACGGCCGTTCTTAAATTGCGATTGACGGATCCATTGTCAG
GAACGACGAAGGAAGTCATTTATCGGACGCCTGAAGCGCTGACGATGCTTGGACCACTTCAATGGGCAACAGCGCCCGCA
AAACGGACCACCACGGGCACGTATGCGTTCGCAAAGTGA

Upstream 100 bases:

>100_bases
TGCGGCACCTAGCCGGTCGGATTCACAAGAACAGTAATTTTAATGGGCCGACATCATTGTTCGACCCATTTTTCGCGGTT
ACGAATTAAAGGAAGGTACT

Downstream 100 bases:

>100_bases
TGCTCGCTTAATAGATCGTGTGATGCTGAAGACAAATAAAGGTGCGCCAACTATCGTAATTTACGAGTAGTTGGCGCACC
TTTTTGAGTTGAATCCAATT

Product: serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MLKLIKQRLVWGLVLTATVSGVLGCNVAAHATSSKADEVKAAYVMDAKSGQVVYAQNADQKLPIASLSKLMTLYLVERAI
EHGQIKWTDAVPIRKNVRKMATSATLSVMPMPAKETFTVRELFAATLVGSSNSGAIALGEYVAGSNDKFIKLMNQQAAKW
HLDASFISASGLDNTDLKNYDLKIKGTSDQDQNLVSARAVTTIAQHLIDAYPDVIQVANQRSVKIHKYTVPTSVEILKGA
EYYDPRVPVDGLKTGYTNQAGACLVATYRQNGHRMIATTLGGAWQFTANNNLRLMLKTTEQYKQVVPKSINYQIPGTQTK
VRLVTKYNSKLWVNSKQPMTQTKIALWPLHRTAPNYLAANTAVLKLRLTDPLSGTTKEVIYRTPEALTMLGPLQWATAPA
KRTTTGTYAFAK

Sequences:

>Translated_412_residues
MLKLIKQRLVWGLVLTATVSGVLGCNVAAHATSSKADEVKAAYVMDAKSGQVVYAQNADQKLPIASLSKLMTLYLVERAI
EHGQIKWTDAVPIRKNVRKMATSATLSVMPMPAKETFTVRELFAATLVGSSNSGAIALGEYVAGSNDKFIKLMNQQAAKW
HLDASFISASGLDNTDLKNYDLKIKGTSDQDQNLVSARAVTTIAQHLIDAYPDVIQVANQRSVKIHKYTVPTSVEILKGA
EYYDPRVPVDGLKTGYTNQAGACLVATYRQNGHRMIATTLGGAWQFTANNNLRLMLKTTEQYKQVVPKSINYQIPGTQTK
VRLVTKYNSKLWVNSKQPMTQTKIALWPLHRTAPNYLAANTAVLKLRLTDPLSGTTKEVIYRTPEALTMLGPLQWATAPA
KRTTTGTYAFAK
>Mature_412_residues
MLKLIKQRLVWGLVLTATVSGVLGCNVAAHATSSKADEVKAAYVMDAKSGQVVYAQNADQKLPIASLSKLMTLYLVERAI
EHGQIKWTDAVPIRKNVRKMATSATLSVMPMPAKETFTVRELFAATLVGSSNSGAIALGEYVAGSNDKFIKLMNQQAAKW
HLDASFISASGLDNTDLKNYDLKIKGTSDQDQNLVSARAVTTIAQHLIDAYPDVIQVANQRSVKIHKYTVPTSVEILKGA
EYYDPRVPVDGLKTGYTNQAGACLVATYRQNGHRMIATTLGGAWQFTANNNLRLMLKTTEQYKQVVPKSINYQIPGTQTK
VRLVTKYNSKLWVNSKQPMTQTKIALWPLHRTAPNYLAANTAVLKLRLTDPLSGTTKEVIYRTPEALTMLGPLQWATAPA
KRTTTGTYAFAK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=247, Percent_Identity=31.9838056680162, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87082022, Length=241, Percent_Identity=29.4605809128631, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786851, Length=249, Percent_Identity=29.718875502008, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 45207; Mature: 45207

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLIKQRLVWGLVLTATVSGVLGCNVAAHATSSKADEVKAAYVMDAKSGQVVYAQNADQ
CHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHCEEEEEEECCCCCEEEECCCCC
KLPIASLSKLMTLYLVERAIEHGQIKWTDAVPIRKNVRKMATSATLSVMPMPAKETFTVR
CCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCEEEEEECCCCCCHHHH
ELFAATLVGSSNSGAIALGEYVAGSNDKFIKLMNQQAAKWHLDASFISASGLDNTDLKNY
HHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEHHHHHHEEEECHHHEECCCCCCCCCCCE
DLKIKGTSDQDQNLVSARAVTTIAQHLIDAYPDVIQVANQRSVKIHKYTVPTSVEILKGA
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEEEEECCCCCEEECCC
EYYDPRVPVDGLKTGYTNQAGACLVATYRQNGHRMIATTLGGAWQFTANNNLRLMLKTTE
CCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCEEEEECCCCEEEEEECHH
QYKQVVPKSINYQIPGTQTKVRLVTKYNSKLWVNSKQPMTQTKIALWPLHRTAPNYLAAN
HHHHHCCCCCEEECCCCCEEEEEEEEECCEEEECCCCCCCCCEEEEEECCCCCCCCEECC
TAVLKLRLTDPLSGTTKEVIYRTPEALTMLGPLQWATAPAKRTTTGTYAFAK
EEEEEEEECCCCCCCCHHHEEECCCHHEEECCCHHCCCCCCCCCCCCEEEEC
>Mature Secondary Structure
MLKLIKQRLVWGLVLTATVSGVLGCNVAAHATSSKADEVKAAYVMDAKSGQVVYAQNADQ
CHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHCEEEEEEECCCCCEEEECCCCC
KLPIASLSKLMTLYLVERAIEHGQIKWTDAVPIRKNVRKMATSATLSVMPMPAKETFTVR
CCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCEEEEEECCCCCCHHHH
ELFAATLVGSSNSGAIALGEYVAGSNDKFIKLMNQQAAKWHLDASFISASGLDNTDLKNY
HHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEHHHHHHEEEECHHHEECCCCCCCCCCCE
DLKIKGTSDQDQNLVSARAVTTIAQHLIDAYPDVIQVANQRSVKIHKYTVPTSVEILKGA
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEEEEECCCCCEEECCC
EYYDPRVPVDGLKTGYTNQAGACLVATYRQNGHRMIATTLGGAWQFTANNNLRLMLKTTE
CCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCEEEEECCCCEEEEEECHH
QYKQVVPKSINYQIPGTQTKVRLVTKYNSKLWVNSKQPMTQTKIALWPLHRTAPNYLAAN
HHHHHCCCCCEEECCCCCEEEEEEEEECCEEEECCCCCCCCCEEEEEECCCCCCCCEECC
TAVLKLRLTDPLSGTTKEVIYRTPEALTMLGPLQWATAPAKRTTTGTYAFAK
EEEEEEEECCCCCCCCHHHEEECCCHHEEECCCHHCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 6768745; 3087956; 6780559; 111240 [H]