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The map label for this gene is gatA

Identifier: 28377926

GI number: 28377926

Start: 1044413

End: 1045876

Strand: Direct

Name: gatA

Synonym: lp_1148

Alternate gene names: 28377926

Gene position: 1044413-1045876 (Clockwise)

Preceding gene: 28377925

Following gene: 28377927

Centisome position: 31.57

GC content: 47.61

Gene sequence:

>1464_bases
ATGACTTATTTCGACCAAGATCTAACTAGTCTGCACGCTGACTTGGTAGCCAAGAAGATCAGTGCCACGGAACTAGCGAA
GGCCACTTTTGCGAATATGAATCAGGTTGACCCTAAGCTAGGGGCGTTCTTGAATTTAAATGAAGAACAGGCCTTACAAC
AGGCGGCTGCTTTGGATCAGGATGGCATCGACGCGAACAACGTTTTTGCTGGGATTCCGATGGCAGTCAAGGATAATATT
GTGACGAAGGGCCTAACGACGACGGCGGCTTCTAAAATGTTGGAAAACTTCGTGCCGGTCTATAATGCCACGGTGGTTGA
CAAACTATTAGCAAGCAACGCGTTGATCGTTGGTAAGACCAACATGGATGAATTTGCCATGGGGGGGTCAACTGAAACGT
CGGCGTTCCATGTCACTCGTAATCCTTGGGACATCAGTCGGGTGCCCGGTGGGTCTTCAGGTGGCTCAGCTGTCGCAGTG
GCTTCTGGACAAGTGCCAGTGGCACTCGGTTCTGATACGGGTGGTTCGATTCGGCAACCATCATCATTTAACGGTATTGT
CGGTATGAAACCAACGTACGGTCGGGTCTCACGCTGGGGGCTGATTGCCTTTGGATCTTCATTAGATCAAATTGGCCCAA
TGACACGGACAGTCAAGGATAACGCGGCCGCTTTGAATATGATTGCAGGTAACGATGTGCATGATACCACGTCATCCAAA
CAAACTGTACCAGACTTTACGGCCGGTTTGACGGGTGATATCAAGGGTATGAAGATCGGGTTACCAAAAGAATACATGGG
CGACGGGATCGATCCTAAAGTTCGTGAAGTCATTCAACAAGCAGTCAAGCAGTTTGAAGACTTAGGTGCTACGGTTGAAG
AAGTCTCCTTACCAAACAGTCGCTACGGTGTGGCTGCTTATTACATCATTGCGTCTTCAGAAGCTTCTTCAAACTTACAA
CGGTTTGACGGGATTCGTTACGGTTTCCGGGCTGACGATGTTAAGAATTTGGAAGATGTCTATGTCCGTAGTCGTTCCGA
GGGCTTTGGTGATGAAGTTAAGCGCCGGATTATGTTAGGGACCTTCTCCTTGTCAGCTGGTTATTACGATGCGTACTTCC
ACAAAGCTGGTCAAGTTCGGACGATGATTATCAATGACTTTAATAAGGTCTTTGAAGATTACGATTTGATTATGGGACCA
GTTGCACCAACGCCGGCCTTTAAGTTGGGTGATGAATTGTCTGATCCAATTACGATGTATATGAACGATGTCTTGACGAT
TCCAGTCAACTTGGCTGGGTTACCAGGAATGTCAGTACCAGCTGGGTTTGCAGACGGGTTACCAGTTGGCTTGCAATTAA
TTGGTAAGGCGTTTGATGAAAGCACAATGTATCGAGCGGGCTATGCCTTTGAACAAGCAACGCAAGTTTACAAGCAAACC
CCGAAAGTCGGAGGTGCTAACTAA

Upstream 100 bases:

>100_bases
ATGAAGCACTCCGGGAAGCATTGCTGAAGAATGCCCCAGAAACGGCCAATGGCTTGATCAAAGTGCCAGCAATTATTGAT
GAAAGTGGGGACGGTGAATA

Downstream 100 bases:

>100_bases
TGAATTTTGTAACCACGATTGGTTTGGAAGTCCACGTTGAATTAAAAACGAACTCAAAAATCTTTAGTCCTTCACCCGTG
CAATTTGGTTCCGAACCAAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MTYFDQDLTSLHADLVAKKISATELAKATFANMNQVDPKLGAFLNLNEEQALQQAAALDQDGIDANNVFAGIPMAVKDNI
VTKGLTTTAASKMLENFVPVYNATVVDKLLASNALIVGKTNMDEFAMGGSTETSAFHVTRNPWDISRVPGGSSGGSAVAV
ASGQVPVALGSDTGGSIRQPSSFNGIVGMKPTYGRVSRWGLIAFGSSLDQIGPMTRTVKDNAAALNMIAGNDVHDTTSSK
QTVPDFTAGLTGDIKGMKIGLPKEYMGDGIDPKVREVIQQAVKQFEDLGATVEEVSLPNSRYGVAAYYIIASSEASSNLQ
RFDGIRYGFRADDVKNLEDVYVRSRSEGFGDEVKRRIMLGTFSLSAGYYDAYFHKAGQVRTMIINDFNKVFEDYDLIMGP
VAPTPAFKLGDELSDPITMYMNDVLTIPVNLAGLPGMSVPAGFADGLPVGLQLIGKAFDESTMYRAGYAFEQATQVYKQT
PKVGGAN

Sequences:

>Translated_487_residues
MTYFDQDLTSLHADLVAKKISATELAKATFANMNQVDPKLGAFLNLNEEQALQQAAALDQDGIDANNVFAGIPMAVKDNI
VTKGLTTTAASKMLENFVPVYNATVVDKLLASNALIVGKTNMDEFAMGGSTETSAFHVTRNPWDISRVPGGSSGGSAVAV
ASGQVPVALGSDTGGSIRQPSSFNGIVGMKPTYGRVSRWGLIAFGSSLDQIGPMTRTVKDNAAALNMIAGNDVHDTTSSK
QTVPDFTAGLTGDIKGMKIGLPKEYMGDGIDPKVREVIQQAVKQFEDLGATVEEVSLPNSRYGVAAYYIIASSEASSNLQ
RFDGIRYGFRADDVKNLEDVYVRSRSEGFGDEVKRRIMLGTFSLSAGYYDAYFHKAGQVRTMIINDFNKVFEDYDLIMGP
VAPTPAFKLGDELSDPITMYMNDVLTIPVNLAGLPGMSVPAGFADGLPVGLQLIGKAFDESTMYRAGYAFEQATQVYKQT
PKVGGAN
>Mature_486_residues
TYFDQDLTSLHADLVAKKISATELAKATFANMNQVDPKLGAFLNLNEEQALQQAAALDQDGIDANNVFAGIPMAVKDNIV
TKGLTTTAASKMLENFVPVYNATVVDKLLASNALIVGKTNMDEFAMGGSTETSAFHVTRNPWDISRVPGGSSGGSAVAVA
SGQVPVALGSDTGGSIRQPSSFNGIVGMKPTYGRVSRWGLIAFGSSLDQIGPMTRTVKDNAAALNMIAGNDVHDTTSSKQ
TVPDFTAGLTGDIKGMKIGLPKEYMGDGIDPKVREVIQQAVKQFEDLGATVEEVSLPNSRYGVAAYYIIASSEASSNLQR
FDGIRYGFRADDVKNLEDVYVRSRSEGFGDEVKRRIMLGTFSLSAGYYDAYFHKAGQVRTMIINDFNKVFEDYDLIMGPV
APTPAFKLGDELSDPITMYMNDVLTIPVNLAGLPGMSVPAGFADGLPVGLQLIGKAFDESTMYRAGYAFEQATQVYKQTP
KVGGAN

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=497, Percent_Identity=37.0221327967807, Blast_Score=310, Evalue=1e-84,
Organism=Homo sapiens, GI195972892, Length=469, Percent_Identity=23.8805970149254, Blast_Score=125, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17543272, Length=429, Percent_Identity=38.6946386946387, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17537465, Length=485, Percent_Identity=23.2989690721649, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=304, Percent_Identity=26.3157894736842, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71990152, Length=268, Percent_Identity=28.3582089552239, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=303, Percent_Identity=25.4125412541254, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=303, Percent_Identity=29.042904290429, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=303, Percent_Identity=29.042904290429, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=421, Percent_Identity=34.2042755344418, Blast_Score=233, Evalue=6e-62,
Organism=Saccharomyces cerevisiae, GI6319685, Length=466, Percent_Identity=29.3991416309013, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6320448, Length=524, Percent_Identity=23.2824427480916, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648113, Length=451, Percent_Identity=41.9068736141907, Blast_Score=308, Evalue=5e-84,
Organism=Drosophila melanogaster, GI24652985, Length=476, Percent_Identity=25.8403361344538, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI19922090, Length=476, Percent_Identity=25.8403361344538, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24652981, Length=476, Percent_Identity=25.8403361344538, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24652983, Length=476, Percent_Identity=25.8403361344538, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI45550774, Length=246, Percent_Identity=31.7073170731707, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648435, Length=246, Percent_Identity=31.7073170731707, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648437, Length=246, Percent_Identity=31.7073170731707, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24644968, Length=499, Percent_Identity=24.6492985971944, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161078093, Length=495, Percent_Identity=24.4444444444444, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24648441, Length=184, Percent_Identity=35.3260869565217, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648439, Length=184, Percent_Identity=35.3260869565217, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21356731, Length=336, Percent_Identity=25.297619047619, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_LACPL (Q88XP7)

Other databases:

- EMBL:   AL935255
- RefSeq:   NP_784818.1
- ProteinModelPortal:   Q88XP7
- SMR:   Q88XP7
- GeneID:   1063235
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1148
- NMPDR:   fig|220668.1.peg.952
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- BioCyc:   LPLA220668:LP_1148-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52089; Mature: 51958

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 77-77 ACT_SITE 152-152 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYFDQDLTSLHADLVAKKISATELAKATFANMNQVDPKLGAFLNLNEEQALQQAAALDQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEECCCHHHHHHHHHHCCC
DGIDANNVFAGIPMAVKDNIVTKGLTTTAASKMLENFVPVYNATVVDKLLASNALIVGKT
CCCCCCCEEECCCHHHHCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEECC
NMDEFAMGGSTETSAFHVTRNPWDISRVPGGSSGGSAVAVASGQVPVALGSDTGGSIRQP
CCHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCCC
SSFNGIVGMKPTYGRVSRWGLIAFGSSLDQIGPMTRTVKDNAAALNMIAGNDVHDTTSSK
CCCCCCCCCCCCCCCHHHCEEEEECCCHHHHCCCHHHHCCCCCEEEEECCCCCCCCCCCC
QTVPDFTAGLTGDIKGMKIGLPKEYMGDGIDPKVREVIQQAVKQFEDLGATVEEVSLPNS
CCCCCHHCCCCCCCCCEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
RYGVAAYYIIASSEASSNLQRFDGIRYGFRADDVKNLEDVYVRSRSEGFGDEVKRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHEEE
TFSLSAGYYDAYFHKAGQVRTMIINDFNKVFEDYDLIMGPVAPTPAFKLGDELSDPITMY
EEECCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCHHHH
MNDVLTIPVNLAGLPGMSVPAGFADGLPVGLQLIGKAFDESTMYRAGYAFEQATQVYKQT
HCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHC
PKVGGAN
CCCCCCC
>Mature Secondary Structure 
TYFDQDLTSLHADLVAKKISATELAKATFANMNQVDPKLGAFLNLNEEQALQQAAALDQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEECCCHHHHHHHHHHCCC
DGIDANNVFAGIPMAVKDNIVTKGLTTTAASKMLENFVPVYNATVVDKLLASNALIVGKT
CCCCCCCEEECCCHHHHCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCEEEEECC
NMDEFAMGGSTETSAFHVTRNPWDISRVPGGSSGGSAVAVASGQVPVALGSDTGGSIRQP
CCHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCCC
SSFNGIVGMKPTYGRVSRWGLIAFGSSLDQIGPMTRTVKDNAAALNMIAGNDVHDTTSSK
CCCCCCCCCCCCCCCHHHCEEEEECCCHHHHCCCHHHHCCCCCEEEEECCCCCCCCCCCC
QTVPDFTAGLTGDIKGMKIGLPKEYMGDGIDPKVREVIQQAVKQFEDLGATVEEVSLPNS
CCCCCHHCCCCCCCCCEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
RYGVAAYYIIASSEASSNLQRFDGIRYGFRADDVKNLEDVYVRSRSEGFGDEVKRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHEEE
TFSLSAGYYDAYFHKAGQVRTMIINDFNKVFEDYDLIMGPVAPTPAFKLGDELSDPITMY
EEECCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCHHHH
MNDVLTIPVNLAGLPGMSVPAGFADGLPVGLQLIGKAFDESTMYRAGYAFEQATQVYKQT
HCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHC
PKVGGAN
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566