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The map label for this gene is truA

Identifier: 28377870

GI number: 28377870

Start: 978088

End: 978876

Strand: Direct

Name: truA

Synonym: lp_1076

Alternate gene names: 28377870

Gene position: 978088-978876 (Clockwise)

Preceding gene: 28377869

Following gene: 28377871

Centisome position: 29.56

GC content: 48.54

Gene sequence:

>789_bases
GTGACCACGCGGTATAAAGTAGTTTTAGCATATGACGGCACCAATTTTGCGGGTTTTCAACGCCAGCCACACCAGCGAAC
TGTCGAGCAGGTCGTCACTAAGGCGGTCAATAAGATGGCGAAAGATCCCGTTGAACCGATTGTGATCTACGGCGCGGGCC
GAACGGATGCTGGTGTACATGCGTTTGGACAGACGTTGCATTTTGACTTGCCGTACGAAATTAATCCAGAAGGGGTTCGG
CGCGGCCTGAACAGCATGTTACCGATGGATACAATCGTAAAGGCCGTTTCCATTGTGCCGAATGATTTTCACGCGCGTTA
TGATACGGTCGGTAAGCGGTACTGGTATCGGGCCTATCAAAATGAATTTGTAGATCCATTTAAACGGCACTATACGGGGC
ATTTTAAGTTCGCTGCGGATATCGACCGAATTCAGCAAGCCATCGGTGACTTAGAAGGACGGCACGATTTCTCGACCTTC
GTTGCGTCCGGTTCACAAGCACATGATCATGTTCGTGAGATCTATTCAGCCAAAGCGTGGGCCTTGCCGGATGAACGCGA
AATTCAGTTTGAATTTTGCGGTAGTGGTTTTTTATATAACCAGGTTCGAATCATGGTGGCTGTTTTGATGGAAATTGGTC
AGGGCCGGCGCGCAGTTGACTGTATTCCAGCGTTACTAGCGGCTAAGGACCGTGAGCAAGCGCGGGGAACGGCTCCCGCG
GCTGGACTTTATATGAAAAAAGTGTATTATGAACAAAATGAATTACAAGCTGATTTGATAAAGTATTGA

Upstream 100 bases:

>100_bases
ATCTTGACCGTTGCATTGATTTTCTTGCGTGCATAAATGAAGACTGATTAACGTGGGCGTCGCACTGATGGCGACCCACG
TTTTTAACTGGAGGGATATT

Downstream 100 bases:

>100_bases
CTTCTGAGGCGAAATTAGGTAGACTAGTCGTTGGTATTGTTTGCCCCACGATAAGCCCCGGAAATCTTATTGAGTGTCAA
ACAAACACAGAAAATGGAGG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MTTRYKVVLAYDGTNFAGFQRQPHQRTVEQVVTKAVNKMAKDPVEPIVIYGAGRTDAGVHAFGQTLHFDLPYEINPEGVR
RGLNSMLPMDTIVKAVSIVPNDFHARYDTVGKRYWYRAYQNEFVDPFKRHYTGHFKFAADIDRIQQAIGDLEGRHDFSTF
VASGSQAHDHVREIYSAKAWALPDEREIQFEFCGSGFLYNQVRIMVAVLMEIGQGRRAVDCIPALLAAKDREQARGTAPA
AGLYMKKVYYEQNELQADLIKY

Sequences:

>Translated_262_residues
MTTRYKVVLAYDGTNFAGFQRQPHQRTVEQVVTKAVNKMAKDPVEPIVIYGAGRTDAGVHAFGQTLHFDLPYEINPEGVR
RGLNSMLPMDTIVKAVSIVPNDFHARYDTVGKRYWYRAYQNEFVDPFKRHYTGHFKFAADIDRIQQAIGDLEGRHDFSTF
VASGSQAHDHVREIYSAKAWALPDEREIQFEFCGSGFLYNQVRIMVAVLMEIGQGRRAVDCIPALLAAKDREQARGTAPA
AGLYMKKVYYEQNELQADLIKY
>Mature_261_residues
TTRYKVVLAYDGTNFAGFQRQPHQRTVEQVVTKAVNKMAKDPVEPIVIYGAGRTDAGVHAFGQTLHFDLPYEINPEGVRR
GLNSMLPMDTIVKAVSIVPNDFHARYDTVGKRYWYRAYQNEFVDPFKRHYTGHFKFAADIDRIQQAIGDLEGRHDFSTFV
ASGSQAHDHVREIYSAKAWALPDEREIQFEFCGSGFLYNQVRIMVAVLMEIGQGRRAVDCIPALLAAKDREQARGTAPAA
GLYMKKVYYEQNELQADLIKY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=278, Percent_Identity=32.7338129496403, Blast_Score=105, Evalue=3e-23,
Organism=Escherichia coli, GI1788657, Length=248, Percent_Identity=35.0806451612903, Blast_Score=139, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321112, Length=228, Percent_Identity=28.5087719298246, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI116007752, Length=308, Percent_Identity=27.5974025974026, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281362146, Length=261, Percent_Identity=25.2873563218391, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648455, Length=261, Percent_Identity=25.2873563218391, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_LACPL (Q88XU9)

Other databases:

- EMBL:   AL935254
- RefSeq:   NP_784762.1
- ProteinModelPortal:   Q88XU9
- GeneID:   1063415
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1076
- NMPDR:   fig|220668.1.peg.896
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   LPLA220668:LP_1076-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29805; Mature: 29674

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: ACT_SITE 56-56

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRYKVVLAYDGTNFAGFQRQPHQRTVEQVVTKAVNKMAKDPVEPIVIYGAGRTDAGVH
CCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHH
AFGQTLHFDLPYEINPEGVRRGLNSMLPMDTIVKAVSIVPNDFHARYDTVGKRYWYRAYQ
HHCCEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
NEFVDPFKRHYTGHFKFAADIDRIQQAIGDLEGRHDFSTFVASGSQAHDHVREIYSAKAW
HHHCCHHHHHCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHC
ALPDEREIQFEFCGSGFLYNQVRIMVAVLMEIGQGRRAVDCIPALLAAKDREQARGTAPA
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHCCCCCH
AGLYMKKVYYEQNELQADLIKY
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTRYKVVLAYDGTNFAGFQRQPHQRTVEQVVTKAVNKMAKDPVEPIVIYGAGRTDAGVH
CCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHH
AFGQTLHFDLPYEINPEGVRRGLNSMLPMDTIVKAVSIVPNDFHARYDTVGKRYWYRAYQ
HHCCEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
NEFVDPFKRHYTGHFKFAADIDRIQQAIGDLEGRHDFSTFVASGSQAHDHVREIYSAKAW
HHHCCHHHHHCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHC
ALPDEREIQFEFCGSGFLYNQVRIMVAVLMEIGQGRRAVDCIPALLAAKDREQARGTAPA
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHCCCCCH
AGLYMKKVYYEQNELQADLIKY
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566