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The map label for this gene is guaA

Identifier: 28377744

GI number: 28377744

Start: 844615

End: 846171

Strand: Direct

Name: guaA

Synonym: lp_0914

Alternate gene names: 28377744

Gene position: 844615-846171 (Clockwise)

Preceding gene: 28377743

Following gene: 28377747

Centisome position: 25.53

GC content: 47.33

Gene sequence:

>1557_bases
TTGGCAAAAACGGATACCGCGTCTTTCGATTCCATTTTGGTATTGGATTTCGGTAGTCAGTATAATCAATTGATTACCCG
ACGCATTCGTGATTTCGGCGTTTATTCGGAACTACTTCCGAACACGATCACCGCTGAAGAAATCAAGGCCCGTCACCCGA
AAGGTATTATCTTTTCTGGTGGCCCCAACAGTGTTTACGATGATGGCGCCTTTCGGGTTGACCCGGAGATTTTCAAGTTG
GGCTTACCAATCTTGGGGATTTGTTACGGCATGCAACTGATGACCTACACGCTTGCGGGCGGGAAGGTCGAATCAGCTGA
TAACCGTGAATACGGCAAGGCGAACATTGACGTGACGAGTGACTCGGCGACCCTATTTAAGGACACGCCAGCTGAACAGT
CAGTGTGGATGAGTCATGGTGACTTGGTTACTCAAGCACCTGATGGTTTTGACGTGGTGGCCACTTCTAAGAACTGCCCG
ATTGCAGCAATTCAAGATGTTGACCGGAAGTTATATGGGATTCAATTCCATGCTGAGGTTCGGAATACGGACTACGGTAA
TGATATTCTACGGCACTTTGCATTTGACGTTTGCCAAGCTGAAGCTAACTGGTCAATGGATGATTTCATTGATATGCAGA
TTGAAAAGATCCGCGCTGAAGTTGGCGACAAAAAGGTCTTACTGGGACTTTCCGGTGGGGTTGATTCCAGTGTCGTCGGT
GTGTTATTGCACAAGGCCATTGGCACGCAATTAACCAGTATTTTCGTTGACCATGGTTTGTTACGGAAGGGCGAAGCTGA
TCAAGTTATGGCGAGCCTAGAAGGTAAGTTCGGCTTGAACATCATCAAAGTTGATGCTAAGGACCGCTTCTTAAGCAAGT
TGGCTGGTGTCAGTGATCCTGAACGGAAACGTAAGATTATTGGTAACGAATTTATCCAAGTGTTTGATGAAGAAGCCACG
AAGTTAAACGGGATGGAATTCCTCGCACAAGGGACCCTCTACACCGATGTGATTGAAAGTGGGACTTCAACGGCCCAAAC
AATCAAGTCACATCATAATGTTGGTGGTTTGCCAGAAGACATGCAGTTCAAGTTGATTGAACCGCTACGGACGTTATTTA
AGGACGAAGCACGGGAACTCGGTGAAAAGCTAGGGATGCCGTCTGACCTTGTTTGGCGTCAACCATTCCCCGGCCCTGGT
CTCGGAATTCGGGTTATTGGTGAGATTACGGAAGATAAGTTAGAAATCGTGCGCGATAGTGACTTTATCTTACGTGATGA
AATCAAAAAAGCCGGCTTGGACCGTGAAATCTGGCAATACTTCACCGTATTACCAGGCATCAAGTCAGTCGGTGTCATGG
GTGACGGCCGGACCTACGATTACACCGTTGGTATTCGGGCCGTGACATCAATCGACGGTATGACCGCCGACTTTGCACGT
ATTCCATGGGATGTCTTACAAAAGATTTCAGTGCGGATCGTCAATGAAGTCGATCACGTTAACCGCATCGTGTACGATGT
TACGAGTAAGCCACCTTCGACAATTGAATGGGAATAG

Upstream 100 bases:

>100_bases
ATATTAGCACCTAAGAAATTTCTCATACTGGTTGACCGAACGTTTAGAAATCAGTATGATAGACCTATTGAGCATTAATT
AATCCAAAGGAGTGAACAAC

Downstream 100 bases:

>100_bases
AATTTATTGAAATTAAACGACTTCTATCCCATTAGAACGAGATTCGTAAGGATTTCAAAGTTGAACAAAGCTGATAAATT
TAAAGAAAATCACTAAAAAA

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MAKTDTASFDSILVLDFGSQYNQLITRRIRDFGVYSELLPNTITAEEIKARHPKGIIFSGGPNSVYDDGAFRVDPEIFKL
GLPILGICYGMQLMTYTLAGGKVESADNREYGKANIDVTSDSATLFKDTPAEQSVWMSHGDLVTQAPDGFDVVATSKNCP
IAAIQDVDRKLYGIQFHAEVRNTDYGNDILRHFAFDVCQAEANWSMDDFIDMQIEKIRAEVGDKKVLLGLSGGVDSSVVG
VLLHKAIGTQLTSIFVDHGLLRKGEADQVMASLEGKFGLNIIKVDAKDRFLSKLAGVSDPERKRKIIGNEFIQVFDEEAT
KLNGMEFLAQGTLYTDVIESGTSTAQTIKSHHNVGGLPEDMQFKLIEPLRTLFKDEARELGEKLGMPSDLVWRQPFPGPG
LGIRVIGEITEDKLEIVRDSDFILRDEIKKAGLDREIWQYFTVLPGIKSVGVMGDGRTYDYTVGIRAVTSIDGMTADFAR
IPWDVLQKISVRIVNEVDHVNRIVYDVTSKPPSTIEWE

Sequences:

>Translated_518_residues
MAKTDTASFDSILVLDFGSQYNQLITRRIRDFGVYSELLPNTITAEEIKARHPKGIIFSGGPNSVYDDGAFRVDPEIFKL
GLPILGICYGMQLMTYTLAGGKVESADNREYGKANIDVTSDSATLFKDTPAEQSVWMSHGDLVTQAPDGFDVVATSKNCP
IAAIQDVDRKLYGIQFHAEVRNTDYGNDILRHFAFDVCQAEANWSMDDFIDMQIEKIRAEVGDKKVLLGLSGGVDSSVVG
VLLHKAIGTQLTSIFVDHGLLRKGEADQVMASLEGKFGLNIIKVDAKDRFLSKLAGVSDPERKRKIIGNEFIQVFDEEAT
KLNGMEFLAQGTLYTDVIESGTSTAQTIKSHHNVGGLPEDMQFKLIEPLRTLFKDEARELGEKLGMPSDLVWRQPFPGPG
LGIRVIGEITEDKLEIVRDSDFILRDEIKKAGLDREIWQYFTVLPGIKSVGVMGDGRTYDYTVGIRAVTSIDGMTADFAR
IPWDVLQKISVRIVNEVDHVNRIVYDVTSKPPSTIEWE
>Mature_517_residues
AKTDTASFDSILVLDFGSQYNQLITRRIRDFGVYSELLPNTITAEEIKARHPKGIIFSGGPNSVYDDGAFRVDPEIFKLG
LPILGICYGMQLMTYTLAGGKVESADNREYGKANIDVTSDSATLFKDTPAEQSVWMSHGDLVTQAPDGFDVVATSKNCPI
AAIQDVDRKLYGIQFHAEVRNTDYGNDILRHFAFDVCQAEANWSMDDFIDMQIEKIRAEVGDKKVLLGLSGGVDSSVVGV
LLHKAIGTQLTSIFVDHGLLRKGEADQVMASLEGKFGLNIIKVDAKDRFLSKLAGVSDPERKRKIIGNEFIQVFDEEATK
LNGMEFLAQGTLYTDVIESGTSTAQTIKSHHNVGGLPEDMQFKLIEPLRTLFKDEARELGEKLGMPSDLVWRQPFPGPGL
GIRVIGEITEDKLEIVRDSDFILRDEIKKAGLDREIWQYFTVLPGIKSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARI
PWDVLQKISVRIVNEVDHVNRIVYDVTSKPPSTIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=564, Percent_Identity=36.7021276595745, Blast_Score=303, Evalue=2e-82,
Organism=Escherichia coli, GI1788854, Length=525, Percent_Identity=51.0476190476191, Blast_Score=553, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI71992710, Length=574, Percent_Identity=37.6306620209059, Blast_Score=310, Evalue=9e-85,
Organism=Caenorhabditis elegans, GI71992717, Length=574, Percent_Identity=37.6306620209059, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI133901714, Length=574, Percent_Identity=37.6306620209059, Blast_Score=310, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6323873, Length=518, Percent_Identity=53.2818532818533, Blast_Score=543, Evalue=1e-155,
Organism=Drosophila melanogaster, GI281365319, Length=548, Percent_Identity=37.4087591240876, Blast_Score=297, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_LACPL (Q88Y74)

Other databases:

- EMBL:   AL935254
- RefSeq:   NP_784636.1
- ProteinModelPortal:   Q88Y74
- SMR:   Q88Y74
- GeneID:   1061959
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0914
- NMPDR:   fig|220668.1.peg.770
- HOGENOM:   HBG391775
- OMA:   VVNAAHS
- ProtClustDB:   PRK00074
- BioCyc:   LPLA220668:LP_0914-MONOMER
- BRENDA:   6.3.5.2
- HAMAP:   MF_00344
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 57458; Mature: 57327

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 88-88 ACT_SITE 177-177 ACT_SITE 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTDTASFDSILVLDFGSQYNQLITRRIRDFGVYSELLPNTITAEEIKARHPKGIIFSG
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEECC
GPNSVYDDGAFRVDPEIFKLGLPILGICYGMQLMTYTLAGGKVESADNREYGKANIDVTS
CCCCCCCCCCEEECHHHHHHCCHHHHHHHHHHHHHHEECCCEECCCCCCCCCCEEEEEEC
DSATLFKDTPAEQSVWMSHGDLVTQAPDGFDVVATSKNCPIAAIQDVDRKLYGIQFHAEV
CCCEEEECCCCCHHHHHCCCCEEECCCCCCEEEEECCCCCCHHHHHHHHHHEEEEEEEEE
RNTDYGNDILRHFAFDVCQAEANWSMDDFIDMQIEKIRAEVGDKKVLLGLSGGVDSSVVG
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
VLLHKAIGTQLTSIFVDHGLLRKGEADQVMASLEGKFGLNIIKVDAKDRFLSKLAGVSDP
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHCCCCCH
ERKRKIIGNEFIQVFDEEATKLNGMEFLAQGTLYTDVIESGTSTAQTIKSHHNVGGLPED
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCC
MQFKLIEPLRTLFKDEARELGEKLGMPSDLVWRQPFPGPGLGIRVIGEITEDKLEIVRDS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCC
DFILRDEIKKAGLDREIWQYFTVLPGIKSVGVMGDGRTYDYTVGIRAVTSIDGMTADFAR
CCHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCCCCCEEEEEEEEEEEECCCCCCCHHHH
IPWDVLQKISVRIVNEVDHVNRIVYDVTSKPPSTIEWE
CCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCC
>Mature Secondary Structure 
AKTDTASFDSILVLDFGSQYNQLITRRIRDFGVYSELLPNTITAEEIKARHPKGIIFSG
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEECC
GPNSVYDDGAFRVDPEIFKLGLPILGICYGMQLMTYTLAGGKVESADNREYGKANIDVTS
CCCCCCCCCCEEECHHHHHHCCHHHHHHHHHHHHHHEECCCEECCCCCCCCCCEEEEEEC
DSATLFKDTPAEQSVWMSHGDLVTQAPDGFDVVATSKNCPIAAIQDVDRKLYGIQFHAEV
CCCEEEECCCCCHHHHHCCCCEEECCCCCCEEEEECCCCCCHHHHHHHHHHEEEEEEEEE
RNTDYGNDILRHFAFDVCQAEANWSMDDFIDMQIEKIRAEVGDKKVLLGLSGGVDSSVVG
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
VLLHKAIGTQLTSIFVDHGLLRKGEADQVMASLEGKFGLNIIKVDAKDRFLSKLAGVSDP
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHCCCCCH
ERKRKIIGNEFIQVFDEEATKLNGMEFLAQGTLYTDVIESGTSTAQTIKSHHNVGGLPED
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCC
MQFKLIEPLRTLFKDEARELGEKLGMPSDLVWRQPFPGPGLGIRVIGEITEDKLEIVRDS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCC
DFILRDEIKKAGLDREIWQYFTVLPGIKSVGVMGDGRTYDYTVGIRAVTSIDGMTADFAR
CCHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCCCCCCEEEEEEEEEEEECCCCCCCHHHH
IPWDVLQKISVRIVNEVDHVNRIVYDVTSKPPSTIEWE
CCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566