Click here to switch to the map view.

The map label for this gene is birA2 [H]

Identifier: 28377696

GI number: 28377696

Start: 793924

End: 794913

Strand: Direct

Name: birA2 [H]

Synonym: lp_0854

Alternate gene names: 28377696

Gene position: 793924-794913 (Clockwise)

Preceding gene: 28377694

Following gene: 28377697

Centisome position: 24.0

GC content: 49.09

Gene sequence:

>990_bases
ATGGCAAACCAAGTCGCAGTTTTACGCTTGTTAATCCAAGCGGCGCCCGCGTACTGTTCGGGTGACTGGTTGGCACAACG
ACTTGCAATCAGTCGAACGGCTATTTGGAAATTAGTGCAAGGATTGCAAGCGGCGGGCCATACTATTGAGAGCCGGCATG
GGCGAGGTTATCGGTATGTGGCCAATAATCAATTGAGTGCGCCGGTGATTGAATACGCGCTTACAGCGTTACCACCGTTT
GATTTGACCGTGCTACCAACGGTTGATTCAACGAATGCCTATTTACGGCGGTTAGTCACGCAGACGCCTTTAGCACTTCC
GAAAATCGTGGTTGCGGATACGCAAACTGCTGGTTACGGTCGGTCGGGGCGGGCATTTTATTCGCCAGCACAGACTGGAA
TTTACATGAGCATTGGGTTACCACTCAGTGACCAGAAGGCGTTAGACGCTGGTTTGTTGACAACGAGTACTGCGGTAGTC
GTTGCCCAGACGCTGCAACAGCTGTTTGCGGTTGACGTTTCATTAAAGTGGGTCAATGATGTGTTAGTCAATCATCACAA
GGTGGTTGGTATCTTATCAGAAGGCATCACTAGTCTTGAAACGGGGCAAATTTCAGCAGTCATTATTGGAATTGGCATTA
ACTTAACAACGACAGCTTTTCCAGCGCTACTAGCGCACAAAGCGGGCGCAGTCGTTGCCAATCCAAATGGTGTGACGCGC
AATCAAGTGATTTGTGAGCTACTGCGGCGCTTTTTCAATAGCTATGGGACTTATCGTAGTGGGACTTTCATGGACGCTTA
TCGGAAACTCTCCATGGTGATCGATCAACAGGTCGAGTTGAAGAGCGGAACGCAAAAGTATCGTGGAGTCGTCACGGATA
TTGATGCACACGGGGCGTTAGTCGTCAAATTAGAGACTGGTCAGAGTCGTCATTTTAGTTCTGGTGAGATTACTAAGGTG
AACCTCTTGTCCGGTGGTTATCATGGCTAG

Upstream 100 bases:

>100_bases
CAGCACAGAAAGATGTAATATTACATTCTTTAAATTCAATTGCGGTTAGTTAACCAATATGAAATAATGGGTTAACTAAT
TAGAGATGGGTGAGATTGAT

Downstream 100 bases:

>100_bases
CAAGTTTGTAAAATCAATTTATCAAGTTACCGAACGAATCGCGTCAATCTTTTAAAATGATTTCTGATTCAATGTTTTAC
TGGTTCATACTGTTGTATAG

Product: biotin--[acetyl-CoA-carboxylase] ligase and biotin operon repressor

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MANQVAVLRLLIQAAPAYCSGDWLAQRLAISRTAIWKLVQGLQAAGHTIESRHGRGYRYVANNQLSAPVIEYALTALPPF
DLTVLPTVDSTNAYLRRLVTQTPLALPKIVVADTQTAGYGRSGRAFYSPAQTGIYMSIGLPLSDQKALDAGLLTTSTAVV
VAQTLQQLFAVDVSLKWVNDVLVNHHKVVGILSEGITSLETGQISAVIIGIGINLTTTAFPALLAHKAGAVVANPNGVTR
NQVICELLRRFFNSYGTYRSGTFMDAYRKLSMVIDQQVELKSGTQKYRGVVTDIDAHGALVVKLETGQSRHFSSGEITKV
NLLSGGYHG

Sequences:

>Translated_329_residues
MANQVAVLRLLIQAAPAYCSGDWLAQRLAISRTAIWKLVQGLQAAGHTIESRHGRGYRYVANNQLSAPVIEYALTALPPF
DLTVLPTVDSTNAYLRRLVTQTPLALPKIVVADTQTAGYGRSGRAFYSPAQTGIYMSIGLPLSDQKALDAGLLTTSTAVV
VAQTLQQLFAVDVSLKWVNDVLVNHHKVVGILSEGITSLETGQISAVIIGIGINLTTTAFPALLAHKAGAVVANPNGVTR
NQVICELLRRFFNSYGTYRSGTFMDAYRKLSMVIDQQVELKSGTQKYRGVVTDIDAHGALVVKLETGQSRHFSSGEITKV
NLLSGGYHG
>Mature_328_residues
ANQVAVLRLLIQAAPAYCSGDWLAQRLAISRTAIWKLVQGLQAAGHTIESRHGRGYRYVANNQLSAPVIEYALTALPPFD
LTVLPTVDSTNAYLRRLVTQTPLALPKIVVADTQTAGYGRSGRAFYSPAQTGIYMSIGLPLSDQKALDAGLLTTSTAVVV
AQTLQQLFAVDVSLKWVNDVLVNHHKVVGILSEGITSLETGQISAVIIGIGINLTTTAFPALLAHKAGAVVANPNGVTRN
QVICELLRRFFNSYGTYRSGTFMDAYRKLSMVIDQQVELKSGTQKYRGVVTDIDAHGALVVKLETGQSRHFSSGEITKVN
LLSGGYHG

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=325, Percent_Identity=29.2307692307692, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 35408; Mature: 35277

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANQVAVLRLLIQAAPAYCSGDWLAQRLAISRTAIWKLVQGLQAAGHTIESRHGRGYRYV
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEE
ANNQLSAPVIEYALTALPPFDLTVLPTVDSTNAYLRRLVTQTPLALPKIVVADTQTAGYG
ECCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
RSGRAFYSPAQTGIYMSIGLPLSDQKALDAGLLTTSTAVVVAQTLQQLFAVDVSLKWVND
CCCCEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLVNHHKVVGILSEGITSLETGQISAVIIGIGINLTTTAFPALLAHKAGAVVANPNGVTR
HHHCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCH
NQVICELLRRFFNSYGTYRSGTFMDAYRKLSMVIDQQVELKSGTQKYRGVVTDIDAHGAL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHCCHHHHCCEEEEECCCCEE
VVKLETGQSRHFSSGEITKVNLLSGGYHG
EEEEECCCCCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure 
ANQVAVLRLLIQAAPAYCSGDWLAQRLAISRTAIWKLVQGLQAAGHTIESRHGRGYRYV
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEE
ANNQLSAPVIEYALTALPPFDLTVLPTVDSTNAYLRRLVTQTPLALPKIVVADTQTAGYG
ECCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
RSGRAFYSPAQTGIYMSIGLPLSDQKALDAGLLTTSTAVVVAQTLQQLFAVDVSLKWVND
CCCCEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLVNHHKVVGILSEGITSLETGQISAVIIGIGINLTTTAFPALLAHKAGAVVANPNGVTR
HHHCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCH
NQVICELLRRFFNSYGTYRSGTFMDAYRKLSMVIDQQVELKSGTQKYRGVVTDIDAHGAL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHCCHHHHCCEEEEECCCCEE
VVKLETGQSRHFSSGEITKVNLLSGGYHG
EEEEECCCCCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]