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The map label for this gene is pox1 [H]

Identifier: 28377692

GI number: 28377692

Start: 787619

End: 789358

Strand: Direct

Name: pox1 [H]

Synonym: lp_0849

Alternate gene names: 28377692

Gene position: 787619-789358 (Clockwise)

Preceding gene: 28377691

Following gene: 28377693

Centisome position: 23.81

GC content: 45.06

Gene sequence:

>1740_bases
ATGGCTAAAATTAGTGGTTCAGATGCCGTATTAAAAGTAATTCAGAAGTGGGGCGTAAAACATATTTATGGTTTGCCCGG
TGGGTCGTTCGATTCAACGATGAACGCCATCTATAATCAACGGAAAACGTTGAAGTACATCCAAGTACGTCACGAAGAAG
CTGGTGCGATTGCCGCTTCCGCTGATTATAAGCTAACTGGAAAGATCGGCGTCTGCTTTGGTTCCGCTGGCCCTGGTGCT
GTCCACTTACTGAACGGATTGTACGATGCTAAGGAAGATGGTATTCCAATGTTAGCAATCGTTGCACAAGTTCCTACTAA
GCGGATGAACATGGACTTCTTCCAAGCAATGAATGAAGAACCGATTTTTGACGATGTGGCTGTTTGGAATCGGACTGCGA
TGACTGCTGAGAGTTTACCGATGATGACTGATGAAGCCATTCGTCAAGCGTATGCCCACAATGGTGTAGCTGTCTTAACG
ATCCCTAAGGACTTTGGTTGGGCTGAAATTGAAGACAACTTTGAAACGAACGCTAGCGTGCACACTGTTAACTATCCTGC
ACCAACCGCTGAATCAGTTGCTGATGCGGTTAAATTAATCAAAGCTGCTAAGTCACCAATGATTTACTTTGGCGTTGGCG
CTAAGGACGCGGCTGAAGAATTAAAAGCTGCTTCTGAAAAGTTTAAGATGCCACTTGTCTCTTCAGTATTAGCGAAGGGG
ATCATTGAAGACGACTATCCCGCTTACTTAGGTTCCACGGGACGGGTCGCACCTAAGCCGGGTGCCGAGATTGGCTTTAG
TACGGATCTAATTCTCTGGGTTGGTAACAATGTTCCATTCAGTATCTTCTTATTTAATAAGAAGGCTAAGGTCATTCAAA
TCGATATTGATAGTGAAAAATTTGGTAAGCGCCACCATACGAACGTTGCTATTCAGGCGGATGCGAAAAAAGCTTTAGCG
GCGATCAATGCAGCTGGTGAAGCGCGCGATGATTCAGCCTTTTATGACGCTGCGGTTGCTGATAAAAAGAACTGGGACAA
ATGGCAAGCCAGCTTCAACGATAGCACTGAATCACCAGTTCGCCCCGAACCAATCTTTGATGTCTTGAACCAAGAAGCTA
GTGACAAAGCTGTTTGGGCTATCGATGTCGGCAATGTTAATATTAACTTTGAACGCTTGATCCGAATGCACGATGACCAG
AAATGGGCAACTTCTGGGATTTATGCAACAATGGGCTTTGGGGTTCCTGCTGCACTGGCTGCTAAAGTCAACTACCCAGA
TCGGGACGTCTACAGTTTGAGCGGTGACGGCGCTTTCGCAATGCTGTCAGAAGAAATCTTGGCACAAGTTAAGTATAACT
TGCACATTATCAACATTGTCTTCAGTAATGAGACCTTAGGCTTTATTGAAGCTGAACAGACTGATGATAGCCATCAACCA
CTCTCTGGTGTCGATTTGCCAGATACTGATTGGGCCAAGGTTGGCGAAGGCTATGGTGCCGTTGGTTATACCGTTCGGAC
CAAGGCTGAATTCAAGCAAGCCCTTGAAGACGCTAAGAAGACGGATAAGCCAGTTGTCATTGATGTGAAGTTAACGCATG
CAATGCCATTTACAACGGAACATATGTATCTCGATGACGCATGGCAAGATAAAGACAAGATTGCTGAATTTGTCAAGAAG
TATGATGCTCAAGCGTTGAAGCCGTTCAGCTACTTCTTAAAACAAGCTCAAACAAACTAA

Upstream 100 bases:

>100_bases
GATAAAATCTATCGATAAAAGCGTTTACATTTTAAAAAATCGTGATTACAATAAAGTCGTAAAGTTGATAATTAAAATAT
ATCAGAGGAGGACATTACTA

Downstream 100 bases:

>100_bases
TTTGGATAGATAATTAACGAAAGAGGACGCGATTATCGCGGCTCTTTTGGTACATAGTATTAAGTGTCGCTTGCCATTGT
GGCGGTGACAACATACCAAG

Product: pyruvate oxidase

Products: NA

Alternate protein names: Pyruvic oxidase; POX [H]

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MAKISGSDAVLKVIQKWGVKHIYGLPGGSFDSTMNAIYNQRKTLKYIQVRHEEAGAIAASADYKLTGKIGVCFGSAGPGA
VHLLNGLYDAKEDGIPMLAIVAQVPTKRMNMDFFQAMNEEPIFDDVAVWNRTAMTAESLPMMTDEAIRQAYAHNGVAVLT
IPKDFGWAEIEDNFETNASVHTVNYPAPTAESVADAVKLIKAAKSPMIYFGVGAKDAAEELKAASEKFKMPLVSSVLAKG
IIEDDYPAYLGSTGRVAPKPGAEIGFSTDLILWVGNNVPFSIFLFNKKAKVIQIDIDSEKFGKRHHTNVAIQADAKKALA
AINAAGEARDDSAFYDAAVADKKNWDKWQASFNDSTESPVRPEPIFDVLNQEASDKAVWAIDVGNVNINFERLIRMHDDQ
KWATSGIYATMGFGVPAALAAKVNYPDRDVYSLSGDGAFAMLSEEILAQVKYNLHIINIVFSNETLGFIEAEQTDDSHQP
LSGVDLPDTDWAKVGEGYGAVGYTVRTKAEFKQALEDAKKTDKPVVIDVKLTHAMPFTTEHMYLDDAWQDKDKIAEFVKK
YDAQALKPFSYFLKQAQTN

Sequences:

>Translated_579_residues
MAKISGSDAVLKVIQKWGVKHIYGLPGGSFDSTMNAIYNQRKTLKYIQVRHEEAGAIAASADYKLTGKIGVCFGSAGPGA
VHLLNGLYDAKEDGIPMLAIVAQVPTKRMNMDFFQAMNEEPIFDDVAVWNRTAMTAESLPMMTDEAIRQAYAHNGVAVLT
IPKDFGWAEIEDNFETNASVHTVNYPAPTAESVADAVKLIKAAKSPMIYFGVGAKDAAEELKAASEKFKMPLVSSVLAKG
IIEDDYPAYLGSTGRVAPKPGAEIGFSTDLILWVGNNVPFSIFLFNKKAKVIQIDIDSEKFGKRHHTNVAIQADAKKALA
AINAAGEARDDSAFYDAAVADKKNWDKWQASFNDSTESPVRPEPIFDVLNQEASDKAVWAIDVGNVNINFERLIRMHDDQ
KWATSGIYATMGFGVPAALAAKVNYPDRDVYSLSGDGAFAMLSEEILAQVKYNLHIINIVFSNETLGFIEAEQTDDSHQP
LSGVDLPDTDWAKVGEGYGAVGYTVRTKAEFKQALEDAKKTDKPVVIDVKLTHAMPFTTEHMYLDDAWQDKDKIAEFVKK
YDAQALKPFSYFLKQAQTN
>Mature_578_residues
AKISGSDAVLKVIQKWGVKHIYGLPGGSFDSTMNAIYNQRKTLKYIQVRHEEAGAIAASADYKLTGKIGVCFGSAGPGAV
HLLNGLYDAKEDGIPMLAIVAQVPTKRMNMDFFQAMNEEPIFDDVAVWNRTAMTAESLPMMTDEAIRQAYAHNGVAVLTI
PKDFGWAEIEDNFETNASVHTVNYPAPTAESVADAVKLIKAAKSPMIYFGVGAKDAAEELKAASEKFKMPLVSSVLAKGI
IEDDYPAYLGSTGRVAPKPGAEIGFSTDLILWVGNNVPFSIFLFNKKAKVIQIDIDSEKFGKRHHTNVAIQADAKKALAA
INAAGEARDDSAFYDAAVADKKNWDKWQASFNDSTESPVRPEPIFDVLNQEASDKAVWAIDVGNVNINFERLIRMHDDQK
WATSGIYATMGFGVPAALAAKVNYPDRDVYSLSGDGAFAMLSEEILAQVKYNLHIINIVFSNETLGFIEAEQTDDSHQPL
SGVDLPDTDWAKVGEGYGAVGYTVRTKAEFKQALEDAKKTDKPVVIDVKLTHAMPFTTEHMYLDDAWQDKDKIAEFVKKY
DAQALKPFSYFLKQAQTN

Specific function: Important for the aerobic growth. Decarboxylates pyruvate in four steps. The energy released is partially stored in acetyl phosphate [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=570, Percent_Identity=25.2631578947368, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI21361361, Length=583, Percent_Identity=21.4408233276158, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1787096, Length=524, Percent_Identity=29.0076335877863, Blast_Score=254, Evalue=9e-69,
Organism=Escherichia coli, GI1790104, Length=540, Percent_Identity=27.7777777777778, Blast_Score=214, Evalue=9e-57,
Organism=Escherichia coli, GI1786717, Length=560, Percent_Identity=26.6071428571429, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI87081685, Length=578, Percent_Identity=25.0865051903114, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1788716, Length=571, Percent_Identity=25.0437828371278, Blast_Score=122, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17531299, Length=585, Percent_Identity=23.4188034188034, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17531301, Length=585, Percent_Identity=23.4188034188034, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17542570, Length=553, Percent_Identity=23.3273056057866, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323755, Length=575, Percent_Identity=24.3478260869565, Blast_Score=163, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6320816, Length=579, Percent_Identity=23.8341968911917, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19922626, Length=562, Percent_Identity=25.2669039145907, Blast_Score=149, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014092
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.3.3 [H]

Molecular weight: Translated: 63498; Mature: 63367

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKISGSDAVLKVIQKWGVKHIYGLPGGSFDSTMNAIYNQRKTLKYIQVRHEEAGAIAAS
CCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHEEEHEEEECCCCEEEEC
ADYKLTGKIGVCFGSAGPGAVHLLNGLYDAKEDGIPMLAIVAQVPTKRMNMDFFQAMNEE
CCEEEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCC
PIFDDVAVWNRTAMTAESLPMMTDEAIRQAYAHNGVAVLTIPKDFGWAEIEDNFETNASV
CCCCHHHHHCCHHCCHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCCEE
HTVNYPAPTAESVADAVKLIKAAKSPMIYFGVGAKDAAEELKAASEKFKMPLVSSVLAKG
EEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHC
IIEDDYPAYLGSTGRVAPKPGAEIGFSTDLILWVGNNVPFSIFLFNKKAKVIQIDIDSEK
CCCCCCCHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCEEEEEEECHHH
FGKRHHTNVAIQADAKKALAAINAAGEARDDSAFYDAAVADKKNWDKWQASFNDSTESPV
HCCCCCCCEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCCCCCCC
RPEPIFDVLNQEASDKAVWAIDVGNVNINFERLIRMHDDQKWATSGIYATMGFGVPAALA
CCCHHHHHHCCCCCCCEEEEEEECCEEECHHHHHHHCCCCCHHHCCEEEEECCCCCCHHE
AKVNYPDRDVYSLSGDGAFAMLSEEILAQVKYNLHIINIVFSNETLGFIEAEQTDDSHQP
EECCCCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEECCCCCCCCC
LSGVDLPDTDWAKVGEGYGAVGYTVRTKAEFKQALEDAKKTDKPVVIDVKLTHAMPFTTE
CCCCCCCCCCHHHHCCCCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCC
HMYLDDAWQDKDKIAEFVKKYDAQALKPFSYFLKQAQTN
EEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure 
AKISGSDAVLKVIQKWGVKHIYGLPGGSFDSTMNAIYNQRKTLKYIQVRHEEAGAIAAS
CCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHEEEHEEEECCCCEEEEC
ADYKLTGKIGVCFGSAGPGAVHLLNGLYDAKEDGIPMLAIVAQVPTKRMNMDFFQAMNEE
CCEEEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCC
PIFDDVAVWNRTAMTAESLPMMTDEAIRQAYAHNGVAVLTIPKDFGWAEIEDNFETNASV
CCCCHHHHHCCHHCCHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCCEE
HTVNYPAPTAESVADAVKLIKAAKSPMIYFGVGAKDAAEELKAASEKFKMPLVSSVLAKG
EEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHC
IIEDDYPAYLGSTGRVAPKPGAEIGFSTDLILWVGNNVPFSIFLFNKKAKVIQIDIDSEK
CCCCCCCHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCEEEEEEECHHH
FGKRHHTNVAIQADAKKALAAINAAGEARDDSAFYDAAVADKKNWDKWQASFNDSTESPV
HCCCCCCCEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCCCCCCC
RPEPIFDVLNQEASDKAVWAIDVGNVNINFERLIRMHDDQKWATSGIYATMGFGVPAALA
CCCHHHHHHCCCCCCCEEEEEEECCEEECHHHHHHHCCCCCHHHCCEEEEECCCCCCHHE
AKVNYPDRDVYSLSGDGAFAMLSEEILAQVKYNLHIINIVFSNETLGFIEAEQTDDSHQP
EECCCCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEECCCCCCCCC
LSGVDLPDTDWAKVGEGYGAVGYTVRTKAEFKQALEDAKKTDKPVVIDVKLTHAMPFTTE
CCCCCCCCCCHHHHCCCCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCC
HMYLDDAWQDKDKIAEFVKKYDAQALKPFSYFLKQAQTN
EEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12566566; 8145244; 8438155 [H]