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The map label for this gene is pbuG [H]

Identifier: 28377691

GI number: 28377691

Start: 785694

End: 787001

Strand: Direct

Name: pbuG [H]

Synonym: lp_0848

Alternate gene names: 28377691

Gene position: 785694-787001 (Clockwise)

Preceding gene: 28377689

Following gene: 28377692

Centisome position: 23.75

GC content: 44.04

Gene sequence:

>1308_bases
ATGAATAAAATTGCGGCATATTTTAATTTTTCAGAATTGAAAACGTCAATGCGAACCGAAGTGCTGGCTGGCTTGACCAC
GTTTGTTTCGATGGCATACATCCTATTTGTTAATCCGAGCGTCCTCGGGGCTTCGGGGATGGATAAGGGCGCCGTCTTCA
CCGCGACGGCCTTAGCGTCAGCGTTAGGCTGTTTATTGATGGGGTTAGTTGCTAAGTACCCGATAGCAATTGCACCAGGA
TTAGGCGTTAACGCGTTCTTTACTTATTCCGTAGTTATCGGAATGGGCATTAAATGGCAAACTGCTCTGGCTGGGGTGTT
CGTTGCAGCCGTGATTTTTATCTTGATTACGATTTTTAAGCTCCGGGAAATGATTATTGATGCGATTCCGCGGGATATGA
AGTTAGCAATTTCGGCGGGGATCGGGTTCTTCATTGCTTTTATTGGTCTTCATGGTGGTGGCTTAATTGTTGCCAACAAA
TCAACGGTTGTTGGTCTGGGATCATTGACAGTTGGAACGACCTGGTTAACTATTTTTGGATTGATCGTCACGTTGATTTT
GATGAGTCGCAAAGTTCCCGGTGGGATTTTTATCGGGATGGTTTTAACCTCCATTTTAGGGCTAGCAACTGGCTTGATTA
AGATGCCATCCACAATTGTTTCGGCAGCACCTTCGTTGAAACCAACGTTTGGTGTGGCGCTTGGTCATATTGGTGATATT
AATTCGTTACAATTGATTATTGTTGTTTTGACGTTCTTATTAGTGACATTCTTCGATACGGCCGGAACGCTAGTTGGATT
GGCAGAACAGGCTGGATTCATGAAAAATAATAAAATGCCGCGGGTTGGTAAAGCGTTGATGGCCGATTCAACTTCGATGT
TAGTTGGGTCAGTGTTGGGAACCTCACCAACTTCAGCTTATGTTGAATCTTCGGCCGGAATTGCGGTCGGTGGTCGCTCT
GGGATGAGTGCAGTTGTTACTGGTGCGCTATTCATTCTTGGATTATTTTTCTCACCGTTATTAAACGTCGTGACGGATCA
AGTTACTGCACCTGCACTGATTATTGTGGGTGTGTTGAGGGCAGAATCTTTAAAAGAGATCAGTTGGGAACGGTTTGAAA
TTGCCGTACCCGCATTTCTAACTGTTATCGGGATGCCGTTGACTTATAGTATTTCTGATGGGATTGCTTTAGGCTTCATT
GCATACGTCATTACGATGGTCGCAACTAAGCATGCCAAGGAAGTTCATCCATTAATGTACGTGCTGTTCTTCATCTTCAT
TATCTTCTTGTGGATATTAAATGTCTGA

Upstream 100 bases:

>100_bases
AAATAAACAAAATGATACGATTAAATACGTTAAAAGAACACTAAAACACGAACGTTTACAATTGTGCAATTGTAATTTAG
GCGAATATTTGGAGGGAATC

Downstream 100 bases:

>100_bases
CAGTTAAAGATGGTCAGCTCGACGTTATTGGCTAGTTACAGATAATTTAAATGACTTAATTCTAAACGGACTACTGACGC
ACATGACGTTGGTAGTCTTT

Product: transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MNKIAAYFNFSELKTSMRTEVLAGLTTFVSMAYILFVNPSVLGASGMDKGAVFTATALASALGCLLMGLVAKYPIAIAPG
LGVNAFFTYSVVIGMGIKWQTALAGVFVAAVIFILITIFKLREMIIDAIPRDMKLAISAGIGFFIAFIGLHGGGLIVANK
STVVGLGSLTVGTTWLTIFGLIVTLILMSRKVPGGIFIGMVLTSILGLATGLIKMPSTIVSAAPSLKPTFGVALGHIGDI
NSLQLIIVVLTFLLVTFFDTAGTLVGLAEQAGFMKNNKMPRVGKALMADSTSMLVGSVLGTSPTSAYVESSAGIAVGGRS
GMSAVVTGALFILGLFFSPLLNVVTDQVTAPALIIVGVLRAESLKEISWERFEIAVPAFLTVIGMPLTYSISDGIALGFI
AYVITMVATKHAKEVHPLMYVLFFIFIIFLWILNV

Sequences:

>Translated_435_residues
MNKIAAYFNFSELKTSMRTEVLAGLTTFVSMAYILFVNPSVLGASGMDKGAVFTATALASALGCLLMGLVAKYPIAIAPG
LGVNAFFTYSVVIGMGIKWQTALAGVFVAAVIFILITIFKLREMIIDAIPRDMKLAISAGIGFFIAFIGLHGGGLIVANK
STVVGLGSLTVGTTWLTIFGLIVTLILMSRKVPGGIFIGMVLTSILGLATGLIKMPSTIVSAAPSLKPTFGVALGHIGDI
NSLQLIIVVLTFLLVTFFDTAGTLVGLAEQAGFMKNNKMPRVGKALMADSTSMLVGSVLGTSPTSAYVESSAGIAVGGRS
GMSAVVTGALFILGLFFSPLLNVVTDQVTAPALIIVGVLRAESLKEISWERFEIAVPAFLTVIGMPLTYSISDGIALGFI
AYVITMVATKHAKEVHPLMYVLFFIFIIFLWILNV
>Mature_435_residues
MNKIAAYFNFSELKTSMRTEVLAGLTTFVSMAYILFVNPSVLGASGMDKGAVFTATALASALGCLLMGLVAKYPIAIAPG
LGVNAFFTYSVVIGMGIKWQTALAGVFVAAVIFILITIFKLREMIIDAIPRDMKLAISAGIGFFIAFIGLHGGGLIVANK
STVVGLGSLTVGTTWLTIFGLIVTLILMSRKVPGGIFIGMVLTSILGLATGLIKMPSTIVSAAPSLKPTFGVALGHIGDI
NSLQLIIVVLTFLLVTFFDTAGTLVGLAEQAGFMKNNKMPRVGKALMADSTSMLVGSVLGTSPTSAYVESSAGIAVGGRS
GMSAVVTGALFILGLFFSPLLNVVTDQVTAPALIIVGVLRAESLKEISWERFEIAVPAFLTVIGMPLTYSISDGIALGFI
AYVITMVATKHAKEVHPLMYVLFFIFIIFLWILNV

Specific function: Involved in the uptake of the purine bases hypoxanthine and guanine [H]

COG id: COG2252

COG function: function code R; Permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. AzgA purine transporter (TC 2.A.1.40) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082309, Length=415, Percent_Identity=42.1686746987952, Blast_Score=330, Evalue=7e-92,
Organism=Escherichia coli, GI1790150, Length=425, Percent_Identity=42.8235294117647, Blast_Score=325, Evalue=5e-90,
Organism=Escherichia coli, GI1790499, Length=423, Percent_Identity=34.7517730496454, Blast_Score=208, Evalue=7e-55,
Organism=Escherichia coli, GI48994909, Length=422, Percent_Identity=34.8341232227488, Blast_Score=206, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 45730; Mature: 45730

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIAAYFNFSELKTSMRTEVLAGLTTFVSMAYILFVNPSVLGASGMDKGAVFTATALAS
CCCEEHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
ALGCLLMGLVAKYPIAIAPGLGVNAFFTYSVVIGMGIKWQTALAGVFVAAVIFILITIFK
HHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LREMIIDAIPRDMKLAISAGIGFFIAFIGLHGGGLIVANKSTVVGLGSLTVGTTWLTIFG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHH
LIVTLILMSRKVPGGIFIGMVLTSILGLATGLIKMPSTIVSAAPSLKPTFGVALGHIGDI
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHCCCCC
NSLQLIIVVLTFLLVTFFDTAGTLVGLAEQAGFMKNNKMPRVGKALMADSTSMLVGSVLG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCHHHHHHHHCCHHHHHHHHHC
TSPTSAYVESSAGIAVGGRSGMSAVVTGALFILGLFFSPLLNVVTDQVTAPALIIVGVLR
CCCCHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AESLKEISWERFEIAVPAFLTVIGMPLTYSISDGIALGFIAYVITMVATKHAKEVHPLMY
HHHHHHCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLFFIFIIFLWILNV
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKIAAYFNFSELKTSMRTEVLAGLTTFVSMAYILFVNPSVLGASGMDKGAVFTATALAS
CCCEEHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
ALGCLLMGLVAKYPIAIAPGLGVNAFFTYSVVIGMGIKWQTALAGVFVAAVIFILITIFK
HHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LREMIIDAIPRDMKLAISAGIGFFIAFIGLHGGGLIVANKSTVVGLGSLTVGTTWLTIFG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHH
LIVTLILMSRKVPGGIFIGMVLTSILGLATGLIKMPSTIVSAAPSLKPTFGVALGHIGDI
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHCCCCC
NSLQLIIVVLTFLLVTFFDTAGTLVGLAEQAGFMKNNKMPRVGKALMADSTSMLVGSVLG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCHHHHHHHHCCHHHHHHHHHC
TSPTSAYVESSAGIAVGGRSGMSAVVTGALFILGLFFSPLLNVVTDQVTAPALIIVGVLR
CCCCHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AESLKEISWERFEIAVPAFLTVIGMPLTYSISDGIALGFIAYVITMVATKHAKEVHPLMY
HHHHHHCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLFFIFIIFLWILNV
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377; 11591660 [H]