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The map label for this gene is metS

Identifier: 28377354

GI number: 28377354

Start: 406690

End: 408741

Strand: Direct

Name: metS

Synonym: lp_0454

Alternate gene names: 28377354

Gene position: 406690-408741 (Clockwise)

Preceding gene: 28377353

Following gene: 28377355

Centisome position: 12.29

GC content: 44.1

Gene sequence:

>2052_bases
ATGGCAGAAACAAAACCAACTTATTATATTACGACGCCGATCTACTATCCTTCGGGGAAGTTGCACATCGGTAATTCATA
CACGACAATTGCGTGTGATACTTTAGCACGTTACAAACGTGCGATGGGTTACGATGTTTACTTCTTAACCGGGACGGATG
AACACGGCCTCAAAATTGAAGAAAAAGCAGAAAAATTGAACACTGATCCGAAGTCATACGTTGATGGGATGGCAAAACAA
ATCAAGGATTTATGGCAATTGCTAGAAATCTCTAATGACAAGTTTATTCGGACCACCGATGATTATCATGAACGCGCGGT
CCAAGAAATTTTCGATCGGTTACTTAAAAATGGTGACATCTACTTAGGTGAGTACGAAGGCTGGTACTCCGTTGATGACG
AAGAATACTTCACTGAAACGCAGCTGGCTGAAGTTTTCCGTGATGATAACGGCAAGGTTATTGGTGGTAAGGCGCCCAGT
GGTCATGAAGTCGAACTTGTTAAAGAACAGTCCTACTTCTTTAAGATGAGTAAGTACGCAGATTGGTTGTTAGATTATTA
TCAAAGTCATCCTGACTTTATTGAACCAGCCAATCGGATGACCGAAATGATCAATAATTTTATTAAACCAGGTCTGGAAG
ACTTGGCCGTTTCTCGGACGAGCTTTACTTGGGGTGTCCCTGTCAAGAGTGATCCTAAGCACGTCGTTTACGTGTGGATT
GATGCCCTGACAAACTATATTACGGCACTGGGTTACGCAACCGGCGACTCTGAAGACTTGTTTAACAAGTTCTGGCCTGC
CGATGTTCAAATGGTTGGTAAAGAAATCGTGCGCTTCCATACGATTTACTGGCCAATTATTTTGCATGCACTCGGGTTAC
CATTACCTAAGAAGGTTTTTGGTCATGGTTGGTTATTGATGAAGGACGGCAAGATGTCCAAGTCTAAGGGTAACGTGATT
TATCCTGAAACATTGGTTGAACGTTACGGCTTGGATGCGCTTCGTTATTATTTAGTTAAAGCCATGCCTTATGGTAACGA
TGGTTTATTCACACCAGAGGACTTTGTGGCGCGGGTTAACTATGACTTGGCTAATGACTTGGGAAACTTGCTGAACCGGA
CCATTGCGATGATCAATAAATATGAAGATGGTCAATTACCAGCATTCAAAGCCGGTGTGACTGAATTTGACGCTGACTTA
GAAGCCACAGCTGCCACGACTATTAAGAACTTCAACGCTTGCATGGACAGCCTGCATTTATCGGATGCGCTGGCTGAAGT
GTGGAAGTTGGTCAGTCGGACGAACAAGTATATTGATGAAACGGCACCTTGGCAATTAGCTAAGAGTGACGATGCGAATG
ATGCTGATAAGTTAGCCAGTGTGATGGCCCATTTAGCAGCTAGTTTGCGGGTGATTGCTAGTTTGATCAGTCCGGTCATG
ACGCATGCACCTAAGGAAATCTTTACCCAATTAGGGTTGGACCCAGCAACTTTAGCAATTGCCGACTTACAACTGGCTGA
TTTGCCGGCTGGCGCACAAGTCGTTGCTAAGGGAACCCCAATCTTCCCACGGGTCGATATGGATGCCGAAGTTGAATTCT
TGAAGGGTAAGATGACGAAATCCGACAAACAGAAGGGGCGTAAAGCCATGGAAAACGCGAAACATGAAGCTGAAGTCGAA
CAAGGCTGGAACCCAGCTGAGACTAATTTGAACCTCACTAAACCAGCAATTACCATTGATGATTTTGACAAGGTCGAATT
GAAAGTTGCTGAAGTCATTACGGTGCAGAAATTGAAAGGGGCTGACAAACTCCTACAATTCCGTTTAGATGCAGGAGATG
CTGATCATCGCCAAATCCTTTCAGGTATTGCTAAGTGGTATCCAGAACCGGAAGAACTGATTGGCAAGAAGGTTATCATC
GTTGGTAACTTAAAGCCACGGAAGCTTCGTGGTGAAATGAGCCAAGGCATGTTACTGTCAGCAGAACACGACGGTCAGGT
GCAATTAATTACGGTACCAGATAATATGGTAAACGGATCATTGATTTCGTAA

Upstream 100 bases:

>100_bases
CCAAGACGTGTTGTTTGGACGCGTGTGATTTGTTCATGGATTTTAATCAAAGACATGCTTAACGAGTTAATCATAAACCA
GTTTGAAGGGAATTTACGTC

Downstream 100 bases:

>100_bases
AAACTAAATGTTGAGTAAAAAATCGGGCGCACTGAATAGTGAGCTCGACTTTTTTATTTGTAATTCAATTGTTATTAGTG
GCTTTTGACAAATGGGTTAA

Product: methionine--tRNA ligase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 683; Mature: 682

Protein sequence:

>683_residues
MAETKPTYYITTPIYYPSGKLHIGNSYTTIACDTLARYKRAMGYDVYFLTGTDEHGLKIEEKAEKLNTDPKSYVDGMAKQ
IKDLWQLLEISNDKFIRTTDDYHERAVQEIFDRLLKNGDIYLGEYEGWYSVDDEEYFTETQLAEVFRDDNGKVIGGKAPS
GHEVELVKEQSYFFKMSKYADWLLDYYQSHPDFIEPANRMTEMINNFIKPGLEDLAVSRTSFTWGVPVKSDPKHVVYVWI
DALTNYITALGYATGDSEDLFNKFWPADVQMVGKEIVRFHTIYWPIILHALGLPLPKKVFGHGWLLMKDGKMSKSKGNVI
YPETLVERYGLDALRYYLVKAMPYGNDGLFTPEDFVARVNYDLANDLGNLLNRTIAMINKYEDGQLPAFKAGVTEFDADL
EATAATTIKNFNACMDSLHLSDALAEVWKLVSRTNKYIDETAPWQLAKSDDANDADKLASVMAHLAASLRVIASLISPVM
THAPKEIFTQLGLDPATLAIADLQLADLPAGAQVVAKGTPIFPRVDMDAEVEFLKGKMTKSDKQKGRKAMENAKHEAEVE
QGWNPAETNLNLTKPAITIDDFDKVELKVAEVITVQKLKGADKLLQFRLDAGDADHRQILSGIAKWYPEPEELIGKKVII
VGNLKPRKLRGEMSQGMLLSAEHDGQVQLITVPDNMVNGSLIS

Sequences:

>Translated_683_residues
MAETKPTYYITTPIYYPSGKLHIGNSYTTIACDTLARYKRAMGYDVYFLTGTDEHGLKIEEKAEKLNTDPKSYVDGMAKQ
IKDLWQLLEISNDKFIRTTDDYHERAVQEIFDRLLKNGDIYLGEYEGWYSVDDEEYFTETQLAEVFRDDNGKVIGGKAPS
GHEVELVKEQSYFFKMSKYADWLLDYYQSHPDFIEPANRMTEMINNFIKPGLEDLAVSRTSFTWGVPVKSDPKHVVYVWI
DALTNYITALGYATGDSEDLFNKFWPADVQMVGKEIVRFHTIYWPIILHALGLPLPKKVFGHGWLLMKDGKMSKSKGNVI
YPETLVERYGLDALRYYLVKAMPYGNDGLFTPEDFVARVNYDLANDLGNLLNRTIAMINKYEDGQLPAFKAGVTEFDADL
EATAATTIKNFNACMDSLHLSDALAEVWKLVSRTNKYIDETAPWQLAKSDDANDADKLASVMAHLAASLRVIASLISPVM
THAPKEIFTQLGLDPATLAIADLQLADLPAGAQVVAKGTPIFPRVDMDAEVEFLKGKMTKSDKQKGRKAMENAKHEAEVE
QGWNPAETNLNLTKPAITIDDFDKVELKVAEVITVQKLKGADKLLQFRLDAGDADHRQILSGIAKWYPEPEELIGKKVII
VGNLKPRKLRGEMSQGMLLSAEHDGQVQLITVPDNMVNGSLIS
>Mature_682_residues
AETKPTYYITTPIYYPSGKLHIGNSYTTIACDTLARYKRAMGYDVYFLTGTDEHGLKIEEKAEKLNTDPKSYVDGMAKQI
KDLWQLLEISNDKFIRTTDDYHERAVQEIFDRLLKNGDIYLGEYEGWYSVDDEEYFTETQLAEVFRDDNGKVIGGKAPSG
HEVELVKEQSYFFKMSKYADWLLDYYQSHPDFIEPANRMTEMINNFIKPGLEDLAVSRTSFTWGVPVKSDPKHVVYVWID
ALTNYITALGYATGDSEDLFNKFWPADVQMVGKEIVRFHTIYWPIILHALGLPLPKKVFGHGWLLMKDGKMSKSKGNVIY
PETLVERYGLDALRYYLVKAMPYGNDGLFTPEDFVARVNYDLANDLGNLLNRTIAMINKYEDGQLPAFKAGVTEFDADLE
ATAATTIKNFNACMDSLHLSDALAEVWKLVSRTNKYIDETAPWQLAKSDDANDADKLASVMAHLAASLRVIASLISPVMT
HAPKEIFTQLGLDPATLAIADLQLADLPAGAQVVAKGTPIFPRVDMDAEVEFLKGKMTKSDKQKGRKAMENAKHEAEVEQ
GWNPAETNLNLTKPAITIDDFDKVELKVAEVITVQKLKGADKLLQFRLDAGDADHRQILSGIAKWYPEPEELIGKKVIIV
GNLKPRKLRGEMSQGMLLSAEHDGQVQLITVPDNMVNGSLIS

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI24308436, Length=549, Percent_Identity=32.9690346083789, Blast_Score=284, Evalue=2e-76,
Organism=Homo sapiens, GI14043022, Length=568, Percent_Identity=26.2323943661972, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI4507947, Length=99, Percent_Identity=38.3838383838384, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI7661872, Length=196, Percent_Identity=30.1020408163265, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788432, Length=702, Percent_Identity=27.7777777777778, Blast_Score=253, Evalue=4e-68,
Organism=Escherichia coli, GI1789455, Length=98, Percent_Identity=36.734693877551, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1786861, Length=204, Percent_Identity=29.9019607843137, Blast_Score=66, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI212645096, Length=523, Percent_Identity=29.8279158699809, Blast_Score=264, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI71992781, Length=413, Percent_Identity=27.3607748184019, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17540794, Length=614, Percent_Identity=25.4071661237785, Blast_Score=162, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6321610, Length=514, Percent_Identity=31.3229571984436, Blast_Score=256, Evalue=8e-69,
Organism=Saccharomyces cerevisiae, GI6321704, Length=555, Percent_Identity=26.1261261261261, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6323414, Length=203, Percent_Identity=29.064039408867, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21358483, Length=506, Percent_Identity=31.6205533596838, Blast_Score=254, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24655311, Length=576, Percent_Identity=26.7361111111111, Blast_Score=165, Evalue=9e-41,
Organism=Drosophila melanogaster, GI21355409, Length=186, Percent_Identity=29.5698924731183, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24651924, Length=102, Percent_Identity=32.3529411764706, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_LACPL (Q88Z97)

Other databases:

- EMBL:   AL935253
- RefSeq:   NP_784246.1
- ProteinModelPortal:   Q88Z97
- GeneID:   1064149
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0454
- NMPDR:   fig|220668.1.peg.380
- HOGENOM:   HBG721667
- OMA:   APHIGHA
- ProtClustDB:   PRK12267
- BioCyc:   LPLA220668:LP_0454-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_01228
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 76797; Mature: 76666

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD; PS00013 PROKAR_LIPOPROTEIN

Important sites: BINDING 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETKPTYYITTPIYYPSGKLHIGNSYTTIACDTLARYKRAMGYDVYFLTGTDEHGLKIE
CCCCCCCEEEECCEEECCCEEEECCCEEEEHHHHHHHHHHHCCCEEEEEECCCCCCCEEH
EKAEKLNTDPKSYVDGMAKQIKDLWQLLEISNDKFIRTTDDYHERAVQEIFDRLLKNGDI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHCCCCE
YLGEYEGWYSVDDEEYFTETQLAEVFRDDNGKVIGGKAPSGHEVELVKEQSYFFKMSKYA
EEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECHHHHHHHHHHH
DWLLDYYQSHPDFIEPANRMTEMINNFIKPGLEDLAVSRTSFTWGVPVKSDPKHVVYVWI
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCEEECCCCCCCCCEEEEEEH
DALTNYITALGYATGDSEDLFNKFWPADVQMVGKEIVRFHTIYWPIILHALGLPLPKKVF
HHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
GHGWLLMKDGKMSKSKGNVIYPETLVERYGLDALRYYLVKAMPYGNDGLFTPEDFVARVN
CCCEEEEECCCCCCCCCCEECHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHHHHCC
YDLANDLGNLLNRTIAMINKYEDGQLPAFKAGVTEFDADLEATAATTIKNFNACMDSLHL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
SDALAEVWKLVSRTNKYIDETAPWQLAKSDDANDADKLASVMAHLAASLRVIASLISPVM
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
THAPKEIFTQLGLDPATLAIADLQLADLPAGAQVVAKGTPIFPRVDMDAEVEFLKGKMTK
HHCHHHHHHHCCCCHHHHEEEEEEHCCCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCC
SDKQKGRKAMENAKHEAEVEQGWNPAETNLNLTKPAITIDDFDKVELKVAEVITVQKLKG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEECCCCCHHHHHHHHHHHHHHCC
ADKLLQFRLDAGDADHRQILSGIAKWYPEPEELIGKKVIIVGNLKPRKLRGEMSQGMLLS
HHHHHHEEECCCCCHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCHHHHHHHCCCEEEE
AEHDGQVQLITVPDNMVNGSLIS
ECCCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
AETKPTYYITTPIYYPSGKLHIGNSYTTIACDTLARYKRAMGYDVYFLTGTDEHGLKIE
CCCCCCEEEECCEEECCCEEEECCCEEEEHHHHHHHHHHHCCCEEEEEECCCCCCCEEH
EKAEKLNTDPKSYVDGMAKQIKDLWQLLEISNDKFIRTTDDYHERAVQEIFDRLLKNGDI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHCCCCE
YLGEYEGWYSVDDEEYFTETQLAEVFRDDNGKVIGGKAPSGHEVELVKEQSYFFKMSKYA
EEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECHHHHHHHHHHH
DWLLDYYQSHPDFIEPANRMTEMINNFIKPGLEDLAVSRTSFTWGVPVKSDPKHVVYVWI
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCEEECCCCCCCCCEEEEEEH
DALTNYITALGYATGDSEDLFNKFWPADVQMVGKEIVRFHTIYWPIILHALGLPLPKKVF
HHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
GHGWLLMKDGKMSKSKGNVIYPETLVERYGLDALRYYLVKAMPYGNDGLFTPEDFVARVN
CCCEEEEECCCCCCCCCCEECHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHHHHCC
YDLANDLGNLLNRTIAMINKYEDGQLPAFKAGVTEFDADLEATAATTIKNFNACMDSLHL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
SDALAEVWKLVSRTNKYIDETAPWQLAKSDDANDADKLASVMAHLAASLRVIASLISPVM
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
THAPKEIFTQLGLDPATLAIADLQLADLPAGAQVVAKGTPIFPRVDMDAEVEFLKGKMTK
HHCHHHHHHHCCCCHHHHEEEEEEHCCCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCC
SDKQKGRKAMENAKHEAEVEQGWNPAETNLNLTKPAITIDDFDKVELKVAEVITVQKLKG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEECCCCCHHHHHHHHHHHHHHCC
ADKLLQFRLDAGDADHRQILSGIAKWYPEPEELIGKKVIIVGNLKPRKLRGEMSQGMLLS
HHHHHHEEECCCCCHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCHHHHHHHCCCEEEE
AEHDGQVQLITVPDNMVNGSLIS
ECCCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566