Click here to switch to the map view.

The map label for this gene is brnQ1 [H]

Identifier: 28377307

GI number: 28377307

Start: 362398

End: 363774

Strand: Direct

Name: brnQ1 [H]

Synonym: lp_0399

Alternate gene names: 28377307

Gene position: 362398-363774 (Clockwise)

Preceding gene: 28377306

Following gene: 28377308

Centisome position: 10.95

GC content: 49.38

Gene sequence:

>1377_bases
ATGCAAATAACGCGAAAATTAACAATCAAACATTACATCATTATTGGATCAATGCTCTTTGGGATGTTTTTTGGGGCTGG
CAACTTGATTTTCCCAATTCACTTAGGGCAGCTAGCGGGGGCCAATTGGTTGACAGCTGGTTGGGGATTCTTGCTGACAG
GGACACTATTGCCACTGTTAGGAATTATCGCCATCAGTGTGACGCACAGTAGTGGGATTTATGAGCTAGCGCGGCCAATT
GGTAAACGCTACGCGACGATTTTTATGGTACTGACTTGTGCGACTTTGGGACCCTTGTTCGCGACGCCGCGGACAGCCAC
TACGCCGTTTCAAATTGGCATCGCTAGCCATGTGAGTTCGGCACAGGCACAGTGGTATTTATTAGCTTACTCATTGATTT
TCTTCAGTATCACTTACTGGGCAGCTCGTAGGCCCACCCGGATCGTCGATACGATCGGTAAATTGTTAAACCCAGCTTTT
CTGATTTTGCTCGCCTTAATTTTTGGGATTGCCTTCAGCCGGCCGTTAGGGACCACGAATGCGACACCGACCGCGGCCTA
CCAACACGGCGCGTTCTTGAACGGCTTTTTGCAAGGGTATAATACGATGGATGCGTTAGCTGCCTTGTTGTTCGGAATTG
CAGTTGTCACCACGATTCGGGGCCTTGGTCAACGGGATGCCGGAGCGATTGCTAAGACGGCCGCCAAGTCTAGTTTGTTG
AGTATTAGTTTAGAAGCAGTCATTTATCTTGGCCTGATCTGGATTGGCGCGACGTCGTTATCGCAATTTAAGTTGTCTGC
GGATGGTGGCATTGCCTTTGGTCAGATTGCCAATGCTTTTATGGGCACGCCGGGTGCGATTGTTTTAGCGATTATGGCGA
CCTTGACGTGTCTGACAACGGCAGTGGGTTTGGCGACGTCGTTTGCGGAAGCCTTGCACGCTAAATTTCCTAAAATCAGT
TATCGCGCTTGGAACCGCTTGGCCTGTGGGGCCTCATTTCTCATCGCTAATATTGGGTTGGATCAAATTATTGCATGGTC
GACGCCGATGCTGATGTTCCTGTATCCGTTGGCGATTACACTGATTATCTTAGCGATTGCCTCACCGCTATTTCATCGTG
ATCCGCTCGTTTATCGATGGACGACGGCGTTAACTTTGATTCCAGCGTTCATTGATGGTCTAGGTGCTGCACCAGCCCTG
ATTACTCGGTTAGGATGGGTTCAAACGATTCTTGCATGGGATCAACGCTACTTACCATTAGCTAAACTAGGATTTGATTG
GGCACTTCCCGCCATTATTGGTCTACTGATCGGCTTGGGCTGGCACTATGCGCGCCGCTTCCAGAACGCCCATGCTTACA
AGATGAGCCAGGACTAA

Upstream 100 bases:

>100_bases
TGCGCTTAACAAGTAGTGATGGTGGTTTGTATCGAGTGCTCTTACTAGTGATTGTTCAAACTGGTTGATGAGAACGACGC
TTAACGGAGGAGAGACGACT

Downstream 100 bases:

>100_bases
AGGTGTATGATAGATGGTAAGACTTTTGATGGATGGGTGAGCACATGGATTTTTTGGGAATACTTTGTTTGTTGATTTTG
GCGACAACCCTCGCTGGGCA

Product: branched-chain amino acid transport protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MQITRKLTIKHYIIIGSMLFGMFFGAGNLIFPIHLGQLAGANWLTAGWGFLLTGTLLPLLGIIAISVTHSSGIYELARPI
GKRYATIFMVLTCATLGPLFATPRTATTPFQIGIASHVSSAQAQWYLLAYSLIFFSITYWAARRPTRIVDTIGKLLNPAF
LILLALIFGIAFSRPLGTTNATPTAAYQHGAFLNGFLQGYNTMDALAALLFGIAVVTTIRGLGQRDAGAIAKTAAKSSLL
SISLEAVIYLGLIWIGATSLSQFKLSADGGIAFGQIANAFMGTPGAIVLAIMATLTCLTTAVGLATSFAEALHAKFPKIS
YRAWNRLACGASFLIANIGLDQIIAWSTPMLMFLYPLAITLIILAIASPLFHRDPLVYRWTTALTLIPAFIDGLGAAPAL
ITRLGWVQTILAWDQRYLPLAKLGFDWALPAIIGLLIGLGWHYARRFQNAHAYKMSQD

Sequences:

>Translated_458_residues
MQITRKLTIKHYIIIGSMLFGMFFGAGNLIFPIHLGQLAGANWLTAGWGFLLTGTLLPLLGIIAISVTHSSGIYELARPI
GKRYATIFMVLTCATLGPLFATPRTATTPFQIGIASHVSSAQAQWYLLAYSLIFFSITYWAARRPTRIVDTIGKLLNPAF
LILLALIFGIAFSRPLGTTNATPTAAYQHGAFLNGFLQGYNTMDALAALLFGIAVVTTIRGLGQRDAGAIAKTAAKSSLL
SISLEAVIYLGLIWIGATSLSQFKLSADGGIAFGQIANAFMGTPGAIVLAIMATLTCLTTAVGLATSFAEALHAKFPKIS
YRAWNRLACGASFLIANIGLDQIIAWSTPMLMFLYPLAITLIILAIASPLFHRDPLVYRWTTALTLIPAFIDGLGAAPAL
ITRLGWVQTILAWDQRYLPLAKLGFDWALPAIIGLLIGLGWHYARRFQNAHAYKMSQD
>Mature_458_residues
MQITRKLTIKHYIIIGSMLFGMFFGAGNLIFPIHLGQLAGANWLTAGWGFLLTGTLLPLLGIIAISVTHSSGIYELARPI
GKRYATIFMVLTCATLGPLFATPRTATTPFQIGIASHVSSAQAQWYLLAYSLIFFSITYWAARRPTRIVDTIGKLLNPAF
LILLALIFGIAFSRPLGTTNATPTAAYQHGAFLNGFLQGYNTMDALAALLFGIAVVTTIRGLGQRDAGAIAKTAAKSSLL
SISLEAVIYLGLIWIGATSLSQFKLSADGGIAFGQIANAFMGTPGAIVLAIMATLTCLTTAVGLATSFAEALHAKFPKIS
YRAWNRLACGASFLIANIGLDQIIAWSTPMLMFLYPLAITLIILAIASPLFHRDPLVYRWTTALTLIPAFIDGLGAAPAL
ITRLGWVQTILAWDQRYLPLAKLGFDWALPAIIGLLIGLGWHYARRFQNAHAYKMSQD

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=446, Percent_Identity=33.4080717488789, Blast_Score=224, Evalue=8e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 49387; Mature: 49387

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITRKLTIKHYIIIGSMLFGMFFGAGNLIFPIHLGQLAGANWLTAGWGFLLTGTLLPLL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHH
GIIAISVTHSSGIYELARPIGKRYATIFMVLTCATLGPLFATPRTATTPFQIGIASHVSS
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHH
AQAQWYLLAYSLIFFSITYWAARRPTRIVDTIGKLLNPAFLILLALIFGIAFSRPLGTTN
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCC
ATPTAAYQHGAFLNGFLQGYNTMDALAALLFGIAVVTTIRGLGQRDAGAIAKTAAKSSLL
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SISLEAVIYLGLIWIGATSLSQFKLSADGGIAFGQIANAFMGTPGAIVLAIMATLTCLTT
HHHHHHHHHHHHHHHCCCCHHHEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AVGLATSFAEALHAKFPKISYRAWNRLACGASFLIANIGLDQIIAWSTPMLMFLYPLAIT
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHH
LIILAIASPLFHRDPLVYRWTTALTLIPAFIDGLGAAPALITRLGWVQTILAWDQRYLPL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCH
AKLGFDWALPAIIGLLIGLGWHYARRFQNAHAYKMSQD
HHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQITRKLTIKHYIIIGSMLFGMFFGAGNLIFPIHLGQLAGANWLTAGWGFLLTGTLLPLL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHH
GIIAISVTHSSGIYELARPIGKRYATIFMVLTCATLGPLFATPRTATTPFQIGIASHVSS
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHH
AQAQWYLLAYSLIFFSITYWAARRPTRIVDTIGKLLNPAFLILLALIFGIAFSRPLGTTN
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCC
ATPTAAYQHGAFLNGFLQGYNTMDALAALLFGIAVVTTIRGLGQRDAGAIAKTAAKSSLL
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SISLEAVIYLGLIWIGATSLSQFKLSADGGIAFGQIANAFMGTPGAIVLAIMATLTCLTT
HHHHHHHHHHHHHHHCCCCHHHEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AVGLATSFAEALHAKFPKISYRAWNRLACGASFLIANIGLDQIIAWSTPMLMFLYPLAIT
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHH
LIILAIASPLFHRDPLVYRWTTALTLIPAFIDGLGAAPALITRLGWVQTILAWDQRYLPL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCH
AKLGFDWALPAIIGLLIGLGWHYARRFQNAHAYKMSQD
HHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8544834 [H]