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The map label for this gene is napA1 [H]

Identifier: 28377308

GI number: 28377308

Start: 363819

End: 365003

Strand: Direct

Name: napA1 [H]

Synonym: lp_0400

Alternate gene names: 28377308

Gene position: 363819-365003 (Clockwise)

Preceding gene: 28377307

Following gene: 28377309

Centisome position: 11.0

GC content: 46.41

Gene sequence:

>1185_bases
ATGGATTTTTTGGGAATACTTTGTTTGTTGATTTTGGCGACAACCCTCGCTGGGCACTTTGCTCACCGTGCGGGGATTCC
TGCCGTGATTGGTGAGATTCTGGTCGGGGTGATATTAGGGCCGGCACTACTTAACTGGGTCCATTTAACGACTTTAGTTC
AAACGTTCTCAGATATTGGGGTCGTGGTACTGATGTTTATCGGTGGACTGGAAAGCAACTTAGCACTTTTACGTAAGTAT
TTACGCCCTGCCATTATCGTGGCTATTATTGGAGTGATCTTTCCAGTCGTTATTATGGGGCTTGCGAGTCGCTTCTTTCA
GTTTAGCTGGTTTGAATCACTGTTTATCGGTGTCATCTTTTCAGCCACGTCGGTTTCGATTTCCGTGGAGGTACTAAAGG
AATTTAAAGTGTTGGATACTAAGGAAGGAGCCACGATTCTAGGGGCAGCGGTCGCTGACGACATTATCGGGGTGATTCTG
TTGAGTATCATGATTGCAATGATGGGTGGTCAGAGTGGCGTTGCCCAGACTAATCTCGGTATCGTGCTCTTGGAACAGGT
TGGCTTCTTCATAGGGGTCTTCATCGTGATTAAGTGGGTTGCCCCGTATCTGATGCGCTTGAGTGATCATCTGTTGATGG
CTGCCAGTCCAACTATTATGGCGATTATTATTTGTTTAGCGTTGGCATGGTTGGCAGAACTAGTGGGCTTGAGTGGTGCT
ATAGGTGCTTTCTTCGCCGGAGTCGCAATTGCACAAACTGATTACCAACACGTGGTGGACGTTAGTATTGAACCAGTGGG
CTACACGACGTTTATTCCATTATTCTTCGTCAGTGTGGGTTTAAATATGACGTTCAAAGGGGTCTGGCAATCGCTACCGT
TCATCGTGGTCATGACGGTGCTCGGTGTGTTAACTAAGTTGTGGGGTTGTGGACTGGGCGCGAAGTTAAATGGGTTCAGT
CGCCATAGTAATTACTTAGTCGGTGCCGGTATGATTTCGCGTGGTGAAATGGCATTGATAACGGCACAAATTGGGTTTTC
AGCGCACTTACTATCAAACGATTACTATTCGGATATTATTTTGGTCATCATTGTGGTGACGATGATTGCCCCGTTCGTCT
TAAAGAATGCCATTCATCGTGCCGGTCAGGTGACTGCAACACATAGCATCATAGAAGAGCATTAA

Upstream 100 bases:

>100_bases
GGCACTATGCGCGCCGCTTCCAGAACGCCCATGCTTACAAGATGAGCCAGGACTAAAGGTGTATGATAGATGGTAAGACT
TTTGATGGATGGGTGAGCAC

Downstream 100 bases:

>100_bases
CCACATAGATTGGGCGGAAGTTAAGAGCCGCTTGGACGCCGTAGTATGGACTACGGCGTTTTTTTGAACCTGTGTGATTT
AACGGAATGAGCGAGGGTGG

Product: Na(+)/H(+) antiporter

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MDFLGILCLLILATTLAGHFAHRAGIPAVIGEILVGVILGPALLNWVHLTTLVQTFSDIGVVVLMFIGGLESNLALLRKY
LRPAIIVAIIGVIFPVVIMGLASRFFQFSWFESLFIGVIFSATSVSISVEVLKEFKVLDTKEGATILGAAVADDIIGVIL
LSIMIAMMGGQSGVAQTNLGIVLLEQVGFFIGVFIVIKWVAPYLMRLSDHLLMAASPTIMAIIICLALAWLAELVGLSGA
IGAFFAGVAIAQTDYQHVVDVSIEPVGYTTFIPLFFVSVGLNMTFKGVWQSLPFIVVMTVLGVLTKLWGCGLGAKLNGFS
RHSNYLVGAGMISRGEMALITAQIGFSAHLLSNDYYSDIILVIIVVTMIAPFVLKNAIHRAGQVTATHSIIEEH

Sequences:

>Translated_394_residues
MDFLGILCLLILATTLAGHFAHRAGIPAVIGEILVGVILGPALLNWVHLTTLVQTFSDIGVVVLMFIGGLESNLALLRKY
LRPAIIVAIIGVIFPVVIMGLASRFFQFSWFESLFIGVIFSATSVSISVEVLKEFKVLDTKEGATILGAAVADDIIGVIL
LSIMIAMMGGQSGVAQTNLGIVLLEQVGFFIGVFIVIKWVAPYLMRLSDHLLMAASPTIMAIIICLALAWLAELVGLSGA
IGAFFAGVAIAQTDYQHVVDVSIEPVGYTTFIPLFFVSVGLNMTFKGVWQSLPFIVVMTVLGVLTKLWGCGLGAKLNGFS
RHSNYLVGAGMISRGEMALITAQIGFSAHLLSNDYYSDIILVIIVVTMIAPFVLKNAIHRAGQVTATHSIIEEH
>Mature_394_residues
MDFLGILCLLILATTLAGHFAHRAGIPAVIGEILVGVILGPALLNWVHLTTLVQTFSDIGVVVLMFIGGLESNLALLRKY
LRPAIIVAIIGVIFPVVIMGLASRFFQFSWFESLFIGVIFSATSVSISVEVLKEFKVLDTKEGATILGAAVADDIIGVIL
LSIMIAMMGGQSGVAQTNLGIVLLEQVGFFIGVFIVIKWVAPYLMRLSDHLLMAASPTIMAIIICLALAWLAELVGLSGA
IGAFFAGVAIAQTDYQHVVDVSIEPVGYTTFIPLFFVSVGLNMTFKGVWQSLPFIVVMTVLGVLTKLWGCGLGAKLNGFS
RHSNYLVGAGMISRGEMALITAQIGFSAHLLSNDYYSDIILVIIVVTMIAPFVLKNAIHRAGQVTATHSIIEEH

Specific function: This is a Na(+)/H(+) antiporter. It can mediate sodium uptake when a transmembrane pH gradient is applied [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family [H]

Homologues:

Organism=Escherichia coli, GI1789749, Length=380, Percent_Identity=26.3157894736842, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786232, Length=286, Percent_Identity=27.972027972028, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786685, Length=373, Percent_Identity=27.6139410187668, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322367, Length=403, Percent_Identity=25.5583126550869, Blast_Score=106, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771 [H]

Pfam domain/function: PF00999 Na_H_Exchanger [H]

EC number: NA

Molecular weight: Translated: 42176; Mature: 42176

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1312090; 1325937 [H]