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The map label for this gene is glpF3 [H]

Identifier: 28377287

GI number: 28377287

Start: 342261

End: 342983

Strand: Direct

Name: glpF3 [H]

Synonym: lp_0372

Alternate gene names: 28377287

Gene position: 342261-342983 (Clockwise)

Preceding gene: 28377286

Following gene: 28377288

Centisome position: 10.35

GC content: 51.45

Gene sequence:

>723_bases
ATGATGAAAGATCCATTAGCACTACAATTGTTGGGGGAATTTCTCGGGACCTTTATCTTGATTCTGTTAGGTGATGGGGT
TGTAGCTGGGGTAACACTCAATAAATCCAAAGCGCAAAATGCGGGCTGGGTCGCGATTACCTTAGGTTGGGGATTTGCAG
TCACGATGGGCGTCTATGCGTCCAGCTTTATGAGCCCCGCACATCTTAATCCGGCGGTCTCACTAGGAATGGCAGTGGCC
GGTAAGTTCCCATGGGCCTACGTGATTCCATATAGTGCGGCTCAGATTGCTGGTGGGGTGATTGGTGGCCTCGTGGTTTG
GTTGCACTACTACCCACACTGGCAAGCAACTAAGGATGCTGGCGCGATTCTCGGCATTTTTGCAACCGGTCCTGGTATCC
GGCGGTATTTCTGGAACTTTATCAGTGAAGTGATTGGAACGTTCGTCCTAGTCTTTGGCTTGTTAGCCTTCACGAAAGGC
CAATTTACGGCGGGATTGAACCCGATTGTTGTCGGAATTCTGATTATCGCTATCGGGCTGTCTCTGGGTGGAACGACCGG
GTATGCAATTAACCCGGCCCGGGATTTAGGGCCCCGCATTGCGCACGCGGTATTGCCCATCGCCAATAAAGGAACTTCTG
ATTGGGCCTACAGTTGGGTGCCAATCGCTGGACCATTGGTTGGTGGCGCGTTAGGCGCACTGTTGTTTAATGTATTGCCA
TAA

Upstream 100 bases:

>100_bases
GGGATGCTGCAACGGTCAAAGCACAAACCGAATTATTGAATCAGACGATTGCTGAATCGGACTTAACAGCACTGAAGCAG
GGCTAAGGAATGGGGTGGTC

Downstream 100 bases:

>100_bases
TTTTATGACTGATTTGATATAAACCGTGAGGACTGTTGGAATTGACAACAGTCCTCACGGTTTTATTTGTGAGTAATGGT
TGAAATGCATGCTTTCATTA

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MMKDPLALQLLGEFLGTFILILLGDGVVAGVTLNKSKAQNAGWVAITLGWGFAVTMGVYASSFMSPAHLNPAVSLGMAVA
GKFPWAYVIPYSAAQIAGGVIGGLVVWLHYYPHWQATKDAGAILGIFATGPGIRRYFWNFISEVIGTFVLVFGLLAFTKG
QFTAGLNPIVVGILIIAIGLSLGGTTGYAINPARDLGPRIAHAVLPIANKGTSDWAYSWVPIAGPLVGGALGALLFNVLP

Sequences:

>Translated_240_residues
MMKDPLALQLLGEFLGTFILILLGDGVVAGVTLNKSKAQNAGWVAITLGWGFAVTMGVYASSFMSPAHLNPAVSLGMAVA
GKFPWAYVIPYSAAQIAGGVIGGLVVWLHYYPHWQATKDAGAILGIFATGPGIRRYFWNFISEVIGTFVLVFGLLAFTKG
QFTAGLNPIVVGILIIAIGLSLGGTTGYAINPARDLGPRIAHAVLPIANKGTSDWAYSWVPIAGPLVGGALGALLFNVLP
>Mature_240_residues
MMKDPLALQLLGEFLGTFILILLGDGVVAGVTLNKSKAQNAGWVAITLGWGFAVTMGVYASSFMSPAHLNPAVSLGMAVA
GKFPWAYVIPYSAAQIAGGVIGGLVVWLHYYPHWQATKDAGAILGIFATGPGIRRYFWNFISEVIGTFVLVFGLLAFTKG
QFTAGLNPIVVGILIIAIGLSLGGTTGYAINPARDLGPRIAHAVLPIANKGTSDWAYSWVPIAGPLVGGALGALLFNVLP

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=251, Percent_Identity=37.0517928286853, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI22538420, Length=254, Percent_Identity=35.8267716535433, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI4826645, Length=253, Percent_Identity=35.5731225296443, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI4502187, Length=254, Percent_Identity=32.2834645669291, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI310133356, Length=181, Percent_Identity=30.939226519337, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI310114181, Length=181, Percent_Identity=30.939226519337, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI4502181, Length=235, Percent_Identity=28.0851063829787, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI4755125, Length=235, Percent_Identity=28.0851063829787, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1790362, Length=248, Percent_Identity=33.4677419354839, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71994009, Length=245, Percent_Identity=33.469387755102, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17533613, Length=243, Percent_Identity=32.0987654320988, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI32564052, Length=243, Percent_Identity=32.0987654320988, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17544068, Length=249, Percent_Identity=33.7349397590361, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71992966, Length=251, Percent_Identity=28.2868525896414, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17531429, Length=251, Percent_Identity=31.0756972111554, Blast_Score=100, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17531431, Length=251, Percent_Identity=31.0756972111554, Blast_Score=100, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71992961, Length=251, Percent_Identity=27.4900398406374, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71993722, Length=235, Percent_Identity=29.3617021276596, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17558372, Length=232, Percent_Identity=27.5862068965517, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6321054, Length=212, Percent_Identity=34.4339622641509, Blast_Score=108, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6322985, Length=291, Percent_Identity=27.8350515463918, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24652747, Length=234, Percent_Identity=29.9145299145299, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45551084, Length=234, Percent_Identity=29.9145299145299, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 25054; Mature: 25054

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKDPLALQLLGEFLGTFILILLGDGVVAGVTLNKSKAQNAGWVAITLGWGFAVTMGVYA
CCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHH
SSFMSPAHLNPAVSLGMAVAGKFPWAYVIPYSAAQIAGGVIGGLVVWLHYYPHWQATKDA
HHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GAILGIFATGPGIRRYFWNFISEVIGTFVLVFGLLAFTKGQFTAGLNPIVVGILIIAIGL
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SLGGTTGYAINPARDLGPRIAHAVLPIANKGTSDWAYSWVPIAGPLVGGALGALLFNVLP
HCCCCCCCEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMKDPLALQLLGEFLGTFILILLGDGVVAGVTLNKSKAQNAGWVAITLGWGFAVTMGVYA
CCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHH
SSFMSPAHLNPAVSLGMAVAGKFPWAYVIPYSAAQIAGGVIGGLVVWLHYYPHWQATKDA
HHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GAILGIFATGPGIRRYFWNFISEVIGTFVLVFGLLAFTKGQFTAGLNPIVVGILIIAIGL
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SLGGTTGYAINPARDLGPRIAHAVLPIANKGTSDWAYSWVPIAGPLVGGALGALLFNVLP
HCCCCCCCEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8436953; 9579061; 9384377; 2127799 [H]