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The map label for this gene is hicD1

Identifier: 28377267

GI number: 28377267

Start: 320360

End: 321319

Strand: Direct

Name: hicD1

Synonym: lp_0350

Alternate gene names: NA

Gene position: 320360-321319 (Clockwise)

Preceding gene: 28377265

Following gene: 28377268

Centisome position: 9.68

GC content: 45.0

Gene sequence:

>960_bases
ATGCGAAAATATGCGATTATTGGGGTCGGCCACGTTGGGGCGACCATTGCTTATACATTAGTTTGTAAAGGAATTGCGGA
TGAATTAGTCTTGATTGATACTAATGCGGCTAAAGCTCGCGCAGAGCAATTAGATTTACAAGACGCTCAAGCCCGTTTAG
ATAGTCGAACGATTATTAAAATCAATGACTATCATGAATTAGACGACACGGATATTTTATTTGTTACTAGCGGCAACATT
CACGCGTTAGACCATGCTTCCGGTAACCGATGGGCTGAATTTGAATATACGAAGCAAATCGTTCAAGATATCGCTCCGAA
AGTTAAAGCAACCAAATTTAATGGCGTGGTCATCGATACAATGAATCCGTGCGACGCAATTACTCACTATTTCCAACGCG
CAACGGGATTATCACGCCAACAGGTCTTTGGGACTGGGACTTTCTTGGATACGGCGCGCATGCAAAAGGTTGTCGCAGAA
GCCTTCGACTGTGATCCAAAAAACATTAGCGGGTATGTTTACGGTGAACACGGCGAATCACAATTCAGCGCGTGGTCAAC
GGTTCAGGTTAATGGTATTCCAATTACATCCTTGGTTGATAAGTATCATTTGGACTTAGATGCACTTGAGGCAGCTGCAC
GCCATGGTGGCTGGGCGGTTCATTCTGGTAAGGGTTATACCAGTTTTGCCATTGCCACTTGCGCGGTTAAATTAAGTGAA
GCAGTCTTTGCAAATGCTCGCTTAGCATGCCCGGTCTCTGCGTATAGTGAGACCTTTGAGACGTATGTTGGTCAGCCAGC
AATCATTGGCAAAGATGGGGTCGAGAGTGTGACGACGCTTGCTCTGACTGCTGATGAACAAGCTAAGTTCCGTAATTCAG
CGGACACGATTATCGAGAAATTCCACGCCTTTGATGACGTCTTAAGCGATCAATCAGTAAAAAAACGTCAACAAGCCTAA

Upstream 100 bases:

>100_bases
TGACCATTACTAACTAGAGCAGTAAGGTTATCACCACATAAATTAAAAGACGATGAGATTATTACATAACGTCTTAATAA
TTAAAACGGGGGTTCAGCTT

Downstream 100 bases:

>100_bases
AAAGTGACGGTTGTTAATTGTCTTGTTTGGCAAACTTCTACCAAAATGTTAAAATTTTACTAAGCAGAACGTTGACTGCA
TTAACTATATGGTGGGATGA

Product: L-2-hydroxyisocaproate dehydrogenase

Products: NA

Alternate protein names: L-HicDH [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MRKYAIIGVGHVGATIAYTLVCKGIADELVLIDTNAAKARAEQLDLQDAQARLDSRTIIKINDYHELDDTDILFVTSGNI
HALDHASGNRWAEFEYTKQIVQDIAPKVKATKFNGVVIDTMNPCDAITHYFQRATGLSRQQVFGTGTFLDTARMQKVVAE
AFDCDPKNISGYVYGEHGESQFSAWSTVQVNGIPITSLVDKYHLDLDALEAAARHGGWAVHSGKGYTSFAIATCAVKLSE
AVFANARLACPVSAYSETFETYVGQPAIIGKDGVESVTTLALTADEQAKFRNSADTIIEKFHAFDDVLSDQSVKKRQQA

Sequences:

>Translated_319_residues
MRKYAIIGVGHVGATIAYTLVCKGIADELVLIDTNAAKARAEQLDLQDAQARLDSRTIIKINDYHELDDTDILFVTSGNI
HALDHASGNRWAEFEYTKQIVQDIAPKVKATKFNGVVIDTMNPCDAITHYFQRATGLSRQQVFGTGTFLDTARMQKVVAE
AFDCDPKNISGYVYGEHGESQFSAWSTVQVNGIPITSLVDKYHLDLDALEAAARHGGWAVHSGKGYTSFAIATCAVKLSE
AVFANARLACPVSAYSETFETYVGQPAIIGKDGVESVTTLALTADEQAKFRNSADTIIEKFHAFDDVLSDQSVKKRQQA
>Mature_319_residues
MRKYAIIGVGHVGATIAYTLVCKGIADELVLIDTNAAKARAEQLDLQDAQARLDSRTIIKINDYHELDDTDILFVTSGNI
HALDHASGNRWAEFEYTKQIVQDIAPKVKATKFNGVVIDTMNPCDAITHYFQRATGLSRQQVFGTGTFLDTARMQKVVAE
AFDCDPKNISGYVYGEHGESQFSAWSTVQVNGIPITSLVDKYHLDLDALEAAARHGGWAVHSGKGYTSFAIATCAVKLSE
AVFANARLACPVSAYSETFETYVGQPAIIGKDGVESVTTLALTADEQAKFRNSADTIIEKFHAFDDVLSDQSVKKRQQA

Specific function: Catalyzes the NADP dependent reversible and stereospecific interconversion between 2-ketocarboxylic acids and L-2-hydroxy-carboxylic acids. 2-ketoacids with medium chain length (five to six C-atoms) are the best substrates [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI5031857, Length=305, Percent_Identity=29.1803278688525, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI260099723, Length=305, Percent_Identity=29.1803278688525, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI47059044, Length=307, Percent_Identity=27.0358306188925, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI221136809, Length=307, Percent_Identity=27.0358306188925, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=29.1803278688525, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=29.1803278688525, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI291575128, Length=305, Percent_Identity=29.5081967213115, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI4557032, Length=305, Percent_Identity=29.5081967213115, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI15082234, Length=307, Percent_Identity=27.0358306188925, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI207028494, Length=305, Percent_Identity=25.5737704918033, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI260099727, Length=204, Percent_Identity=29.9019607843137, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI260099725, Length=204, Percent_Identity=29.9019607843137, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17535107, Length=295, Percent_Identity=27.7966101694915, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI45550422, Length=313, Percent_Identity=30.6709265175719, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI17136226, Length=305, Percent_Identity=25.5737704918033, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: NA

Molecular weight: Translated: 34819; Mature: 34819

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKYAIIGVGHVGATIAYTLVCKGIADELVLIDTNAAKARAEQLDLQDAQARLDSRTIIK
CCEEEEEEECHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHCCHHHHHHHCCCCEEEE
INDYHELDDTDILFVTSGNIHALDHASGNRWAEFEYTKQIVQDIAPKVKATKFNGVVIDT
EECCCCCCCCCEEEEECCCEEEEECCCCCCEEHHHHHHHHHHHHCCCCEEEEECCEEEEC
MNPCDAITHYFQRATGLSRQQVFGTGTFLDTARMQKVVAEAFDCDPKNISGYVYGEHGES
CCHHHHHHHHHHHHCCCCHHHEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
QFSAWSTVQVNGIPITSLVDKYHLDLDALEAAARHGGWAVHSGKGYTSFAIATCAVKLSE
CCCCEEEEEECCEEHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHH
AVFANARLACPVSAYSETFETYVGQPAIIGKDGVESVTTLALTADEQAKFRNSADTIIEK
HHHCCCEEECCHHHHHHHHHHHCCCCCEECCCCCCHHEEEEEECCHHHHHCCHHHHHHHH
FHAFDDVLSDQSVKKRQQA
HHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MRKYAIIGVGHVGATIAYTLVCKGIADELVLIDTNAAKARAEQLDLQDAQARLDSRTIIK
CCEEEEEEECHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHCCHHHHHHHCCCCEEEE
INDYHELDDTDILFVTSGNIHALDHASGNRWAEFEYTKQIVQDIAPKVKATKFNGVVIDT
EECCCCCCCCCEEEEECCCEEEEECCCCCCEEHHHHHHHHHHHHCCCCEEEEECCEEEEC
MNPCDAITHYFQRATGLSRQQVFGTGTFLDTARMQKVVAEAFDCDPKNISGYVYGEHGES
CCHHHHHHHHHHHHCCCCHHHEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
QFSAWSTVQVNGIPITSLVDKYHLDLDALEAAARHGGWAVHSGKGYTSFAIATCAVKLSE
CCCCEEEEEECCEEHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHH
AVFANARLACPVSAYSETFETYVGQPAIIGKDGVESVTTLALTADEQAKFRNSADTIIEK
HHHCCCEEECCHHHHHHHHHHHCCCCCEECCCCCCHHEEEEEECCHHHHHCCHHHHHHHH
FHAFDDVLSDQSVKKRQQA
HHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2684788; 7643402 [H]