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The map label for this gene is birA1 [H]

Identifier: 28377257

GI number: 28377257

Start: 308883

End: 309854

Strand: Direct

Name: birA1 [H]

Synonym: lp_0337

Alternate gene names: 28377257

Gene position: 308883-309854 (Clockwise)

Preceding gene: 28377256

Following gene: 28377258

Centisome position: 9.34

GC content: 48.35

Gene sequence:

>972_bases
ATGACAACTAAAGAACAGGTCTTAACAATTTTATTGCGTCATCATGGTGAGTGGCAATCGGGGGACCAGCTTGCCCAATC
AGTTGGCTGTAGTCGTGAGAGTATTTGGAAGGCAATCAATGCTTTGAGAAAGCAAGGACACCAGATTGAGAGTCGTAAAA
ACCGGGGCTACTGTTATCTGGGAAGTCGTAATTTGGATGCCAAAGCCATTCAAATCTATGGGCAACGTCAATTTACGGGG
CCCCTTGAAGTGGTCGCCCAGACGCCGTCCACTCAGATTCTAGCTAAGAGCTTTTTAAGTCATCATCAGCCACAGTTTGC
CGCCTTCTTCGCGGACGAACAGACGGCTGGCTATGGCCGTTTGGGCCGGGACTTCTATTCACCGGCCAAAACTGGACTGT
ATTTTAGCTTGGTTGTTCCTAATCCCACGAATGCATTGGTCAATGTGGGTTTACTGACAACTGGTGTCGCGGTGAGTGTC
TTGAATGTTTTACAGCAATTTTATCCTAATAAGAACTTTGGGTTGAAATGGGTCAATGATATTTATCTTGATCAGCATAA
GGTCGGGGGCATTATCACGGAAGCAAGTTTGGAATTAGAATCGATTTCCGCAACGGCCTTTATTGTCGGTGTGGGGCTCA
ATTTAACGACCACCGATTTTCCACGGGAATTGCAAGCTCGTGCCCAAGCGGTCGACCCGAGCATGTCAGTGGATCGCAAT
CGGTTAGCAGCGGCCTTATTATCAGCACTAGTAGCGCTATCAAAACGCTACGCTACGGCCGACTTTTTGCCAGCATATCG
GGCTAAATCATTAGTGTTGGGTCAACAAGTGACACTTCAAGTGGGCGATTCCGTGGTGAATGGGGTGGCTGAAGATATCG
ATGAACATGGTGGCTTAGTGGTCCGCATGTTGACCGGGGAGCGGCGGACATTTACGAGTGGTGAAGTGATCAGGGTAGGG
TTACCAGTATGA

Upstream 100 bases:

>100_bases
ACTTTGGCCGTATTTATTGCCCGGCGTGTGCGGGCCATCGTTGATTTTGGATTGCCAGCTTAGTAAATAGCTAATGTGAT
TGGCAGGTCAGGAGGTCATA

Downstream 100 bases:

>100_bases
CTGAGTGGGTACCAGTGATTTACGGCATCGGTTTAGACGCTGCCGGTCGGTGCCAACATTATCACCTTGAAACTGATATC
GCGGCATTGTGGTGTCGGCG

Product: biotin--[acetyl-CoA-carboxylase] ligase and biotin operon repressor

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTTKEQVLTILLRHHGEWQSGDQLAQSVGCSRESIWKAINALRKQGHQIESRKNRGYCYLGSRNLDAKAIQIYGQRQFTG
PLEVVAQTPSTQILAKSFLSHHQPQFAAFFADEQTAGYGRLGRDFYSPAKTGLYFSLVVPNPTNALVNVGLLTTGVAVSV
LNVLQQFYPNKNFGLKWVNDIYLDQHKVGGIITEASLELESISATAFIVGVGLNLTTTDFPRELQARAQAVDPSMSVDRN
RLAAALLSALVALSKRYATADFLPAYRAKSLVLGQQVTLQVGDSVVNGVAEDIDEHGGLVVRMLTGERRTFTSGEVIRVG
LPV

Sequences:

>Translated_323_residues
MTTKEQVLTILLRHHGEWQSGDQLAQSVGCSRESIWKAINALRKQGHQIESRKNRGYCYLGSRNLDAKAIQIYGQRQFTG
PLEVVAQTPSTQILAKSFLSHHQPQFAAFFADEQTAGYGRLGRDFYSPAKTGLYFSLVVPNPTNALVNVGLLTTGVAVSV
LNVLQQFYPNKNFGLKWVNDIYLDQHKVGGIITEASLELESISATAFIVGVGLNLTTTDFPRELQARAQAVDPSMSVDRN
RLAAALLSALVALSKRYATADFLPAYRAKSLVLGQQVTLQVGDSVVNGVAEDIDEHGGLVVRMLTGERRTFTSGEVIRVG
LPV
>Mature_322_residues
TTKEQVLTILLRHHGEWQSGDQLAQSVGCSRESIWKAINALRKQGHQIESRKNRGYCYLGSRNLDAKAIQIYGQRQFTGP
LEVVAQTPSTQILAKSFLSHHQPQFAAFFADEQTAGYGRLGRDFYSPAKTGLYFSLVVPNPTNALVNVGLLTTGVAVSVL
NVLQQFYPNKNFGLKWVNDIYLDQHKVGGIITEASLELESISATAFIVGVGLNLTTTDFPRELQARAQAVDPSMSVDRNR
LAAALLSALVALSKRYATADFLPAYRAKSLVLGQQVTLQVGDSVVNGVAEDIDEHGGLVVRMLTGERRTFTSGEVIRVGL
PV

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=317, Percent_Identity=28.0757097791798, Blast_Score=101, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 35319; Mature: 35188

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKEQVLTILLRHHGEWQSGDQLAQSVGCSRESIWKAINALRKQGHQIESRKNRGYCYL
CCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHCCCCCEEEE
GSRNLDAKAIQIYGQRQFTGPLEVVAQTPSTQILAKSFLSHHQPQFAAFFADEQTAGYGR
CCCCCCCEEEEEECCCCCCCHHHHEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCH
LGRDFYSPAKTGLYFSLVVPNPTNALVNVGLLTTGVAVSVLNVLQQFYPNKNFGLKWVND
HCHHHHCCCCCCEEEEEEECCCCHHHEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHH
IYLDQHKVGGIITEASLELESISATAFIVGVGLNLTTTDFPRELQARAQAVDPSMSVDRN
HHHCHHHHCCEEEECCCCHHHHCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCHH
RLAAALLSALVALSKRYATADFLPAYRAKSLVLGQQVTLQVGDSVVNGVAEDIDEHGGLV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHCCCEE
VRMLTGERRTFTSGEVIRVGLPV
EEEECCCCCEECCCCEEEEECCC
>Mature Secondary Structure 
TTKEQVLTILLRHHGEWQSGDQLAQSVGCSRESIWKAINALRKQGHQIESRKNRGYCYL
CCHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHCCCCCEEEE
GSRNLDAKAIQIYGQRQFTGPLEVVAQTPSTQILAKSFLSHHQPQFAAFFADEQTAGYGR
CCCCCCCEEEEEECCCCCCCHHHHEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCH
LGRDFYSPAKTGLYFSLVVPNPTNALVNVGLLTTGVAVSVLNVLQQFYPNKNFGLKWVND
HCHHHHCCCCCCEEEEEEECCCCHHHEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHH
IYLDQHKVGGIITEASLELESISATAFIVGVGLNLTTTDFPRELQARAQAVDPSMSVDRN
HHHCHHHHCCEEEECCCCHHHHCEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCHH
RLAAALLSALVALSKRYATADFLPAYRAKSLVLGQQVTLQVGDSVVNGVAEDIDEHGGLV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHCCCEE
VRMLTGERRTFTSGEVIRVGLPV
EEEECCCCCEECCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]