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The map label for this gene is pts2A [H]

Identifier: 28377162

GI number: 28377162

Start: 212878

End: 213327

Strand: Direct

Name: pts2A [H]

Synonym: lp_0232

Alternate gene names: 28377162

Gene position: 212878-213327 (Clockwise)

Preceding gene: 28377161

Following gene: 28377163

Centisome position: 6.43

GC content: 44.44

Gene sequence:

>450_bases
ATGGAAGCTTTAGATAAGAAAATGATTGCGTTGAACCAGCAAGTGGCTACGCAAGAAGAAGGCATCCGCTTAGCCGGTCA
ACTATTGGTCGATGGCGGTTGTGTCGAACCAGAATACATTGATGCAATGCAGGCTCGTAATCAGGATGTCTCCGTATATA
TGGGTAATTTCATTGCCATCCCCCATGGTACTGAAGATGGCATGAAGTATATTAAAAAGACCGGGATTTCCGTGGTCCAA
GTACCAATGGGTGTGAGCTGGGGTAATCCAGATGATAACGATGATGATAAGACTGTAACGGTTATCTTCGGGATCGCTGG
GTTGAACGGTGAACACTTGAACCTCTTGTCTCAGATTGCCATATACTGCAGCGACGTTGCCAACGTTGCTAAGTTAGCGG
ACGCTCAATCTGAAGATGAAATCATAAATCTATTAAAGGAAGTTGATTAG

Upstream 100 bases:

>100_bases
CCAATACGCAGACGTTTATGACTGGCGATCGCCAGCAACTTTATCAATTAATTAGTAAAGCATTTTTGAACGAACTAACG
AAATAGCGTAGGTGAAAACA

Downstream 100 bases:

>100_bases
CATGTTAGACGTACATTTTGGCGCAGGGAATATTGGTCGGGGCTTCATTGGCGAAACCTTGGCTGACAACGGGTTTAAGA
TTACTTTCGTTGATGTTAAC

Product: mannitol PTS, EIIA

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Mtl; EIII-Mtl; PTS system mannitol-specific EIIA component [H]

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MEALDKKMIALNQQVATQEEGIRLAGQLLVDGGCVEPEYIDAMQARNQDVSVYMGNFIAIPHGTEDGMKYIKKTGISVVQ
VPMGVSWGNPDDNDDDKTVTVIFGIAGLNGEHLNLLSQIAIYCSDVANVAKLADAQSEDEIINLLKEVD

Sequences:

>Translated_149_residues
MEALDKKMIALNQQVATQEEGIRLAGQLLVDGGCVEPEYIDAMQARNQDVSVYMGNFIAIPHGTEDGMKYIKKTGISVVQ
VPMGVSWGNPDDNDDDKTVTVIFGIAGLNGEHLNLLSQIAIYCSDVANVAKLADAQSEDEIINLLKEVD
>Mature_149_residues
MEALDKKMIALNQQVATQEEGIRLAGQLLVDGGCVEPEYIDAMQARNQDVSVYMGNFIAIPHGTEDGMKYIKKTGISVVQ
VPMGVSWGNPDDNDDDKTVTVIFGIAGLNGEHLNLLSQIAIYCSDVANVAKLADAQSEDEIINLLKEVD

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790027, Length=137, Percent_Identity=38.6861313868613, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1788494, Length=128, Percent_Identity=40.625, Blast_Score=93, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16138; Mature: 16138

Theoretical pI: Translated: 4.00; Mature: 4.00

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEALDKKMIALNQQVATQEEGIRLAGQLLVDGGCVEPEYIDAMQARNQDVSVYMGNFIAI
CCCHHHHHHHHHHHHHHHHCCHHHHHHEEECCCCCCHHHHHHHHHCCCCEEEEECCEEEE
PHGTEDGMKYIKKTGISVVQVPMGVSWGNPDDNDDDKTVTVIFGIAGLNGEHLNLLSQIA
CCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
IYCSDVANVAKLADAQSEDEIINLLKEVD
HHHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MEALDKKMIALNQQVATQEEGIRLAGQLLVDGGCVEPEYIDAMQARNQDVSVYMGNFIAI
CCCHHHHHHHHHHHHHHHHCCHHHHHHEEECCCCCCHHHHHHHHHCCCCEEEEECCEEEE
PHGTEDGMKYIKKTGISVVQVPMGVSWGNPDDNDDDKTVTVIFGIAGLNGEHLNLLSQIA
CCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
IYCSDVANVAKLADAQSEDEIINLLKEVD
HHHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1904856; 12663927 [H]