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The map label for this gene is sacA [H]

Identifier: 28377126

GI number: 28377126

Start: 167690

End: 169195

Strand: Direct

Name: sacA [H]

Synonym: lp_0187

Alternate gene names: 28377126

Gene position: 167690-169195 (Clockwise)

Preceding gene: 28377123

Following gene: 28377127

Centisome position: 5.07

GC content: 48.94

Gene sequence:

>1506_bases
ATGATATGGAATCGTAAAACCCGTTATACCCCTTATGAACAGTGGCCAGCAACTAAGCTACCACAGCTCGTTGCCCAGGT
ACGCCAGTCTAAGTGGCGGATGCAACATCATATTCAGCCTACATCAGGTTTGTTAAATGACCCCAATGGCTTTTCATACT
TTGATGGTCAGTGGCACCTGTTCTACCAAGTCTTTCCGTTCGGTCCGGTACACGGACTGAAGTCATGGCAACACGTGACC
TCTAAGAACTTGGTGGATTGGCATGATGAGGGCTTAGCGATTCGACCAGATACGCCGTACGACTCACACGGCGCGTACAC
GGGTACAGCACTACCAATTGATGATCAGTTATTTATTATGTATACGGGTAATGTCCGTACTGCCGACTGGCAACGTGAAT
CGTATCAATTAGGTGCCTGGATGGACACGGATAATCACATTAAAAAGCTGTCCCGGCCACTAATTGCGCATGCTCCGGCT
GGGTATACGAGTTCGTTTCGTGATCCGGACTTGATCCGGACTGACCACGGCTACTACGCGCTAATCGGTGCGCAGACGAC
TACTGAGATCGGTGCTATCTTGGTTTATTTCTCAAAGGATCTGACCACCTGGACGTGTCAAGGCGAGTTGAACGTTCCCG
CAAATGCGCGGGGTTATATGATTGAGTGTCCAAATCTGGTCTGGATCGATCAACAGCCCGTCTTATTGTTCTGTCCCCAA
GGGTTATCACAAACAACGACCCCGTATCAGAATATTTATCCGAACATGTACCTAGTTGCCGATCAATTGGATCTGGCTCA
GGCACAATTCACTGAACCCCACGCCCTGACGCAATTGGATGACGGCTTTGACGTGTATGCCACGCAGGCAATCAATGCGC
CGGATGGCCGCGCGTTAGCCGTCAGCTGGATTGGGTTACCTGAAATTTCCTATCCAACTGATCGTGAAAATTGGGCCCAT
TGTTTGAGCTTAGTTAAGGAACTAACGCTTAAAGATGGGCACCTCTATCAAAATCCAGTGGCAGCGGTGGATGATTTGCG
GACGACGGCGCATGACCTAGTCTTTGAGCAACAACGGGCGACGGTGGCCGCTTTGAATGGCAGCTTTGAACTCTTACTGA
CGGTTCCCGCTGATAAAACGGTCACGGTTAACATTGCTGATCAGCAGGAAAGTGGCCAGCTGCAGGTGACTGTTGATGCC
AACCATGGTCAGGTTATGATTGATCGGCGGCATACGGGGAATTCGTTTGCTGAAGATTATGGTCAGACTCGTCAAGTTGA
GTTAACGGCCCATAAGACGATAAAAATCCGGCTAATCATAGATGTGTCAGTCTTTGAATGCTATATTGATAATGGCTATT
CCGTGATGACCGGCCGTTTCTTCTTAAACGCTACCCCGAGTCGCCTTAACGTTCAAGGTGACACTACGGCAGTTACCGGT
AAAGTCTGGGAATGGCGCCAATCAGAGCATACTGGAGTCGATAACAATGAAACCAAAATTAAATGA

Upstream 100 bases:

>100_bases
ATGAAAATATAATATAAAAATGTCAAACGATTGACATATCAGTGTAAGCGGTTTTATAATAGTGACAATTAAGTCGAACG
TTAAAAAGGAGCTCATCGTC

Downstream 100 bases:

>100_bases
TGTTGCGAAGCTAGCGGGGGTCTCAGCGACAACCGTTTCTCGGGTTATTAATAACCATGGCTACCTGAGTAGTCAGACTA
AAGAAAAAGTTTTCGCGGCG

Product: beta-fructofuranosidase

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MIWNRKTRYTPYEQWPATKLPQLVAQVRQSKWRMQHHIQPTSGLLNDPNGFSYFDGQWHLFYQVFPFGPVHGLKSWQHVT
SKNLVDWHDEGLAIRPDTPYDSHGAYTGTALPIDDQLFIMYTGNVRTADWQRESYQLGAWMDTDNHIKKLSRPLIAHAPA
GYTSSFRDPDLIRTDHGYYALIGAQTTTEIGAILVYFSKDLTTWTCQGELNVPANARGYMIECPNLVWIDQQPVLLFCPQ
GLSQTTTPYQNIYPNMYLVADQLDLAQAQFTEPHALTQLDDGFDVYATQAINAPDGRALAVSWIGLPEISYPTDRENWAH
CLSLVKELTLKDGHLYQNPVAAVDDLRTTAHDLVFEQQRATVAALNGSFELLLTVPADKTVTVNIADQQESGQLQVTVDA
NHGQVMIDRRHTGNSFAEDYGQTRQVELTAHKTIKIRLIIDVSVFECYIDNGYSVMTGRFFLNATPSRLNVQGDTTAVTG
KVWEWRQSEHTGVDNNETKIK

Sequences:

>Translated_501_residues
MIWNRKTRYTPYEQWPATKLPQLVAQVRQSKWRMQHHIQPTSGLLNDPNGFSYFDGQWHLFYQVFPFGPVHGLKSWQHVT
SKNLVDWHDEGLAIRPDTPYDSHGAYTGTALPIDDQLFIMYTGNVRTADWQRESYQLGAWMDTDNHIKKLSRPLIAHAPA
GYTSSFRDPDLIRTDHGYYALIGAQTTTEIGAILVYFSKDLTTWTCQGELNVPANARGYMIECPNLVWIDQQPVLLFCPQ
GLSQTTTPYQNIYPNMYLVADQLDLAQAQFTEPHALTQLDDGFDVYATQAINAPDGRALAVSWIGLPEISYPTDRENWAH
CLSLVKELTLKDGHLYQNPVAAVDDLRTTAHDLVFEQQRATVAALNGSFELLLTVPADKTVTVNIADQQESGQLQVTVDA
NHGQVMIDRRHTGNSFAEDYGQTRQVELTAHKTIKIRLIIDVSVFECYIDNGYSVMTGRFFLNATPSRLNVQGDTTAVTG
KVWEWRQSEHTGVDNNETKIK
>Mature_501_residues
MIWNRKTRYTPYEQWPATKLPQLVAQVRQSKWRMQHHIQPTSGLLNDPNGFSYFDGQWHLFYQVFPFGPVHGLKSWQHVT
SKNLVDWHDEGLAIRPDTPYDSHGAYTGTALPIDDQLFIMYTGNVRTADWQRESYQLGAWMDTDNHIKKLSRPLIAHAPA
GYTSSFRDPDLIRTDHGYYALIGAQTTTEIGAILVYFSKDLTTWTCQGELNVPANARGYMIECPNLVWIDQQPVLLFCPQ
GLSQTTTPYQNIYPNMYLVADQLDLAQAQFTEPHALTQLDDGFDVYATQAINAPDGRALAVSWIGLPEISYPTDRENWAH
CLSLVKELTLKDGHLYQNPVAAVDDLRTTAHDLVFEQQRATVAALNGSFELLLTVPADKTVTVNIADQQESGQLQVTVDA
NHGQVMIDRRHTGNSFAEDYGQTRQVELTAHKTIKIRLIIDVSVFECYIDNGYSVMTGRFFLNATPSRLNVQGDTTAVTG
KVWEWRQSEHTGVDNNETKIK

Specific function: Unknown

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=504, Percent_Identity=22.8174603174603, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 56724; Mature: 56724

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWNRKTRYTPYEQWPATKLPQLVAQVRQSKWRMQHHIQPTSGLLNDPNGFSYFDGQWHL
CCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCEEE
FYQVFPFGPVHGLKSWQHVTSKNLVDWHDEGLAIRPDTPYDSHGAYTGTALPIDDQLFIM
EEEEECCCCCHHHHHHHHHHCCCCEEECCCCEEECCCCCCCCCCCCCCEECCCCCEEEEE
YTGNVRTADWQRESYQLGAWMDTDNHIKKLSRPLIAHAPAGYTSSFRDPDLIRTDHGYYA
EECCEEECCCCCCCEEEEEEECCHHHHHHHCCCEEEECCCCCCCCCCCCCCEEECCCEEE
LIGAQTTTEIGAILVYFSKDLTTWTCQGELNVPANARGYMIECPNLVWIDQQPVLLFCPQ
EEECCCCCCCEEEEEEEECCCEEEEEECEECCCCCCCEEEEECCCEEEECCCCEEEECCC
GLSQTTTPYQNIYPNMYLVADQLDLAQAQFTEPHALTQLDDGFDVYATQAINAPDGRALA
CCCCCCCCHHHHCCCEEEEECCCHHHHHHCCCCCHHEECCCCCEEEEEEEECCCCCCEEE
VSWIGLPEISYPTDRENWAHCLSLVKELTLKDGHLYQNPVAAVDDLRTTAHDLVFEQQRA
EEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHCCC
TVAALNGSFELLLTVPADKTVTVNIADQQESGQLQVTVDANHGQVMIDRRHTGNSFAEDY
EEEEECCCEEEEEECCCCCEEEEEEECCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHC
GQTRQVELTAHKTIKIRLIIDVSVFECYIDNGYSVMTGRFFLNATPSRLNVQGDTTAVTG
CCCEEEEEEEEEEEEEEEEEEEEEEEEEECCCEEEEEEEEEEECCCCEEEECCCCEEEEC
KVWEWRQSEHTGVDNNETKIK
CEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIWNRKTRYTPYEQWPATKLPQLVAQVRQSKWRMQHHIQPTSGLLNDPNGFSYFDGQWHL
CCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCEEE
FYQVFPFGPVHGLKSWQHVTSKNLVDWHDEGLAIRPDTPYDSHGAYTGTALPIDDQLFIM
EEEEECCCCCHHHHHHHHHHCCCCEEECCCCEEECCCCCCCCCCCCCCEECCCCCEEEEE
YTGNVRTADWQRESYQLGAWMDTDNHIKKLSRPLIAHAPAGYTSSFRDPDLIRTDHGYYA
EECCEEECCCCCCCEEEEEEECCHHHHHHHCCCEEEECCCCCCCCCCCCCCEEECCCEEE
LIGAQTTTEIGAILVYFSKDLTTWTCQGELNVPANARGYMIECPNLVWIDQQPVLLFCPQ
EEECCCCCCCEEEEEEEECCCEEEEEECEECCCCCCCEEEEECCCEEEECCCCEEEECCC
GLSQTTTPYQNIYPNMYLVADQLDLAQAQFTEPHALTQLDDGFDVYATQAINAPDGRALA
CCCCCCCCHHHHCCCEEEEECCCHHHHHHCCCCCHHEECCCCCEEEEEEEECCCCCCEEE
VSWIGLPEISYPTDRENWAHCLSLVKELTLKDGHLYQNPVAAVDDLRTTAHDLVFEQQRA
EEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHCCC
TVAALNGSFELLLTVPADKTVTVNIADQQESGQLQVTVDANHGQVMIDRRHTGNSFAEDY
EEEEECCCEEEEEECCCCCEEEEEEECCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHC
GQTRQVELTAHKTIKIRLIIDVSVFECYIDNGYSVMTGRFFLNATPSRLNVQGDTTAVTG
CCCEEEEEEEEEEEEEEEEEEEEEEEEEECCCEEEEEEEEEEECCCCEEEECCCCEEEEC
KVWEWRQSEHTGVDNNETKIK
CEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA