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The map label for this gene is sacR [H]

Identifier: 28377127

GI number: 28377127

Start: 169176

End: 170156

Strand: Direct

Name: sacR [H]

Synonym: lp_0188

Alternate gene names: 28377127

Gene position: 169176-170156 (Clockwise)

Preceding gene: 28377126

Following gene: 28377128

Centisome position: 5.11

GC content: 43.43

Gene sequence:

>981_bases
ATGAAACCAAAATTAAATGATGTTGCGAAGCTAGCGGGGGTCTCAGCGACAACCGTTTCTCGGGTTATTAATAACCATGG
CTACCTGAGTAGTCAGACTAAAGAAAAAGTTTTCGCGGCGATGCGAGAACTGCATTATCAGCCGAATAATATGGCACGCT
CACTGCAAGGAAAGAATACGCGCTTAATTGGCGTCATCTTTTCAGATATTAGTAATCCGTTCTTTGGTGAACTGGTGTCC
CGGATTGAAAAAATCTTATTTGCTAAAAATTATAAGGTGATTTTATGTAATAGTGCGGATGATCCGCAAAAAGAACGCGA
CTACCTACAAATGTTGATGGCTAATCAGGTCGATGGGATTATTGCTGGTGCCCACAATTTAGGTATTGAAGAATATCAAC
AATACGGTCTTCCGATTATTTCATTTGACCGGTACTTATCCGACAATATTCCCATCGTTAGTTCGGATAATTACCAGGGG
GGCTGGCTAGCAACCCAGACATTGCATCAGGCGGGGGCGACTAACGTGGCTATTTTTACTGGTAAGTCACACGCTGGTTC
GCCAACGAATGGTCGCCGTGAAGGATATGAAGCCTACTTGACTGCCCAACAGCTAACACCCCATGTCCATGAATTACCGT
TTGAATTGACCCCCGCGCTGAAGATGATGGAAATTAAGACGATTATGACACAACATCAGTATGATGGTATTTTTTGTAGT
GATGATTTGACCGCTTTACTAGTGCTCAACGTCGCGCGACAACTGTCATTAACGGTTCCTGAGCAATTACGCGTTGTTGG
TTATGATGGCACGGCGCTCATACGTGATTATCATTCTGAATTAACAACAGTAGAACAACCGTTAGCGGATATTAGCACGT
TACTGGTTTCTCTACTATTGCAGCGGATTGAGGATGCGAATTGTACACTGGAATCAAAATATACGTTACCAGTTAAGCTC
ATCAAGGGCCTTACCGCGTAA

Upstream 100 bases:

>100_bases
AACGCTACCCCGAGTCGCCTTAACGTTCAAGGTGACACTACGGCAGTTACCGGTAAAGTCTGGGAATGGCGCCAATCAGA
GCATACTGGAGTCGATAACA

Downstream 100 bases:

>100_bases
CTAAGGAGTTAATCGTAATTATGGCAGCAACAATCAAGTGGTGGCAACAAGCCGTTGTCTATCAGGTCTATCCACGGAGT
TTTCAAGATACCAATCATGA

Product: sucrose operon repressor

Products: NA

Alternate protein names: Scr operon regulatory protein [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKPKLNDVAKLAGVSATTVSRVINNHGYLSSQTKEKVFAAMRELHYQPNNMARSLQGKNTRLIGVIFSDISNPFFGELVS
RIEKILFAKNYKVILCNSADDPQKERDYLQMLMANQVDGIIAGAHNLGIEEYQQYGLPIISFDRYLSDNIPIVSSDNYQG
GWLATQTLHQAGATNVAIFTGKSHAGSPTNGRREGYEAYLTAQQLTPHVHELPFELTPALKMMEIKTIMTQHQYDGIFCS
DDLTALLVLNVARQLSLTVPEQLRVVGYDGTALIRDYHSELTTVEQPLADISTLLVSLLLQRIEDANCTLESKYTLPVKL
IKGLTA

Sequences:

>Translated_326_residues
MKPKLNDVAKLAGVSATTVSRVINNHGYLSSQTKEKVFAAMRELHYQPNNMARSLQGKNTRLIGVIFSDISNPFFGELVS
RIEKILFAKNYKVILCNSADDPQKERDYLQMLMANQVDGIIAGAHNLGIEEYQQYGLPIISFDRYLSDNIPIVSSDNYQG
GWLATQTLHQAGATNVAIFTGKSHAGSPTNGRREGYEAYLTAQQLTPHVHELPFELTPALKMMEIKTIMTQHQYDGIFCS
DDLTALLVLNVARQLSLTVPEQLRVVGYDGTALIRDYHSELTTVEQPLADISTLLVSLLLQRIEDANCTLESKYTLPVKL
IKGLTA
>Mature_326_residues
MKPKLNDVAKLAGVSATTVSRVINNHGYLSSQTKEKVFAAMRELHYQPNNMARSLQGKNTRLIGVIFSDISNPFFGELVS
RIEKILFAKNYKVILCNSADDPQKERDYLQMLMANQVDGIIAGAHNLGIEEYQQYGLPIISFDRYLSDNIPIVSSDNYQG
GWLATQTLHQAGATNVAIFTGKSHAGSPTNGRREGYEAYLTAQQLTPHVHELPFELTPALKMMEIKTIMTQHQYDGIFCS
DDLTALLVLNVARQLSLTVPEQLRVVGYDGTALIRDYHSELTTVEQPLADISTLLVSLLLQRIEDANCTLESKYTLPVKL
IKGLTA

Specific function: Negative regulator of scrB expression [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=309, Percent_Identity=29.7734627831715, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1790194, Length=327, Percent_Identity=32.1100917431193, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1788474, Length=314, Percent_Identity=27.7070063694268, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1789202, Length=300, Percent_Identity=29.3333333333333, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1790369, Length=307, Percent_Identity=28.0130293159609, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789068, Length=310, Percent_Identity=27.0967741935484, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790715, Length=319, Percent_Identity=27.5862068965517, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI48994940, Length=313, Percent_Identity=24.9201277955272, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787580, Length=293, Percent_Identity=27.3037542662116, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787906, Length=255, Percent_Identity=30.9803921568627, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786268, Length=316, Percent_Identity=27.2151898734177, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1786540, Length=340, Percent_Identity=28.5294117647059, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790689, Length=295, Percent_Identity=24.7457627118644, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36232; Mature: 36232

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPKLNDVAKLAGVSATTVSRVINNHGYLSSQTKEKVFAAMRELHYQPNNMARSLQGKNT
CCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
RLIGVIFSDISNPFFGELVSRIEKILFAKNYKVILCNSADDPQKERDYLQMLMANQVDGI
EEEEEEEHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCE
IAGAHNLGIEEYQQYGLPIISFDRYLSDNIPIVSSDNYQGGWLATQTLHQAGATNVAIFT
EECHHHCCHHHHHHHCCCEEEHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCEEEEEE
GKSHAGSPTNGRREGYEAYLTAQQLTPHVHELPFELTPALKMMEIKTIMTQHQYDGIFCS
CCCCCCCCCCCCCCCHHHEEEHHHHCCHHHHCCCHHCCHHHHHHHHHHHHHHHCCCEEEC
DDLTALLVLNVARQLSLTVPEQLRVVGYDGTALIRDYHSELTTVEQPLADISTLLVSLLL
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRIEDANCTLESKYTLPVKLIKGLTA
HHHCCCCCEEECCCCCHHHHHCCCCC
>Mature Secondary Structure
MKPKLNDVAKLAGVSATTVSRVINNHGYLSSQTKEKVFAAMRELHYQPNNMARSLQGKNT
CCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
RLIGVIFSDISNPFFGELVSRIEKILFAKNYKVILCNSADDPQKERDYLQMLMANQVDGI
EEEEEEEHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCE
IAGAHNLGIEEYQQYGLPIISFDRYLSDNIPIVSSDNYQGGWLATQTLHQAGATNVAIFT
EECHHHCCHHHHHHHCCCEEEHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCEEEEEE
GKSHAGSPTNGRREGYEAYLTAQQLTPHVHELPFELTPALKMMEIKTIMTQHQYDGIFCS
CCCCCCCCCCCCCCCHHHEEEHHHHCCHHHHCCCHHCCHHHHHHHHHHHHHHHCCCEEEC
DDLTALLVLNVARQLSLTVPEQLRVVGYDGTALIRDYHSELTTVEQPLADISTLLVSLLL
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRIEDANCTLESKYTLPVKLIKGLTA
HHHCCCCCEEECCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA