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The map label for this gene is yvdE [H]

Identifier: 28377113

GI number: 28377113

Start: 148314

End: 149276

Strand: Direct

Name: yvdE [H]

Synonym: lp_0173

Alternate gene names: 28377113

Gene position: 148314-149276 (Clockwise)

Preceding gene: 28377112

Following gene: 28377114

Centisome position: 4.48

GC content: 45.59

Gene sequence:

>963_bases
ATGATAACGCTAAACGATGTCGCGAAAAAAGCTAATGTCTCAAAAATGACAGTTTCACGAGTCATTAATCATCCGGAACA
GGTTCGTCCTGAGCTTCGAGAGATGATTTTAAATGTGATGACACAGCTAGACTATCATCCGAATGCGGCAGCGCGAGCGC
TAGTAGGGCGGAGAACCCGGGTTGTAAAATTAACGATTTTTGAAGACATGGACACGACGGAACCCTATTACATGATGTTG
CTCGCGGGGATTGCGAATGAATTGAATCAACATCAATACGCCTTGCAGATTGCGACGCGCAATAGCTATGAGGTTGGCGA
ATGTGATGGCTATATTTTGACTGGTATTCGGCAAGCCGATTACGCATGGGTCGAACGTCTTAATCAGCCAGTCGTCTTTG
TTGGTAGCAATCGTCAGGGATACGACTACGTTGATACTAATAATTATCAGGCGGTCTGGGAAAGTACACGTTATGCCGCG
GAACAGGGCTATACGCATCTTGTCTTTATTGGTATCGCTATTGCCGAACCGTTTGAGCTAGATCGTGAACGAGGTTTTCG
AGAGTACGTCACGACACATCATATTAGCGCTCAGATTATTCGATTAGCCAATCATTCACACGTTGCAACTGCGTATGTGA
AGAGCCATTGGTCTGGTTTTCCACGTAATACGGCGTTTATTTGTGCAAGTGATCGGTTGGCGATCGGCGTTGAAACGGCA
ATTATTAATCAAGGTGGTCGCGTTCCCATAGAATATGGCATTATCGGTTTTGATGGCGTGTTATTAAATCAGATTGCAGC
CAAACGGTTGACAACGATGAAGCAACCGCTTGAAGCAATGGGACGAGCTGCTGCACGGCTATTATTGGCTAATATCAACC
ATACTGATCGTTCTCGCGGGCGGCATGATCAAGAAAAAATCATCATTAATTCACGGTTGTCGGTGGCTGAATCGACCCGT
TAA

Upstream 100 bases:

>100_bases
AATTATCAACGGCAGTAATTGTGCCTCATCAAATTGTTAAACGCCATTCCGTCCACCAAATCAAGTCCTTACAGTAGTAA
GTTAGGGGGGTATTTTGATT

Downstream 100 bases:

>100_bases
AGAGGAGTCTGGATAGATGAGCAAGCATTGGTATGATCAACAAACGATTTATCAAATATATCCTAAGAGTTTTAACGATA
GTAATCACGATGGCATTGGT

Product: transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MITLNDVAKKANVSKMTVSRVINHPEQVRPELREMILNVMTQLDYHPNAAARALVGRRTRVVKLTIFEDMDTTEPYYMML
LAGIANELNQHQYALQIATRNSYEVGECDGYILTGIRQADYAWVERLNQPVVFVGSNRQGYDYVDTNNYQAVWESTRYAA
EQGYTHLVFIGIAIAEPFELDRERGFREYVTTHHISAQIIRLANHSHVATAYVKSHWSGFPRNTAFICASDRLAIGVETA
IINQGGRVPIEYGIIGFDGVLLNQIAAKRLTTMKQPLEAMGRAAARLLLANINHTDRSRGRHDQEKIIINSRLSVAESTR

Sequences:

>Translated_320_residues
MITLNDVAKKANVSKMTVSRVINHPEQVRPELREMILNVMTQLDYHPNAAARALVGRRTRVVKLTIFEDMDTTEPYYMML
LAGIANELNQHQYALQIATRNSYEVGECDGYILTGIRQADYAWVERLNQPVVFVGSNRQGYDYVDTNNYQAVWESTRYAA
EQGYTHLVFIGIAIAEPFELDRERGFREYVTTHHISAQIIRLANHSHVATAYVKSHWSGFPRNTAFICASDRLAIGVETA
IINQGGRVPIEYGIIGFDGVLLNQIAAKRLTTMKQPLEAMGRAAARLLLANINHTDRSRGRHDQEKIIINSRLSVAESTR
>Mature_320_residues
MITLNDVAKKANVSKMTVSRVINHPEQVRPELREMILNVMTQLDYHPNAAARALVGRRTRVVKLTIFEDMDTTEPYYMML
LAGIANELNQHQYALQIATRNSYEVGECDGYILTGIRQADYAWVERLNQPVVFVGSNRQGYDYVDTNNYQAVWESTRYAA
EQGYTHLVFIGIAIAEPFELDRERGFREYVTTHHISAQIIRLANHSHVATAYVKSHWSGFPRNTAFICASDRLAIGVETA
IINQGGRVPIEYGIIGFDGVLLNQIAAKRLTTMKQPLEAMGRAAARLLLANINHTDRSRGRHDQEKIIINSRLSVAESTR

Specific function: Repressor Of The Galactose Operon. Binds Galactose As An Inducer. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=308, Percent_Identity=26.9480519480519, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790369, Length=318, Percent_Identity=27.6729559748428, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788474, Length=309, Percent_Identity=25.2427184466019, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI48994940, Length=306, Percent_Identity=25.8169934640523, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787948, Length=339, Percent_Identity=26.2536873156342, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787580, Length=309, Percent_Identity=24.2718446601942, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790715, Length=323, Percent_Identity=25.077399380805, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789456, Length=338, Percent_Identity=27.2189349112426, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789068, Length=311, Percent_Identity=22.8295819935691, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790689, Length=328, Percent_Identity=23.1707317073171, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36192; Mature: 36192

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLNDVAKKANVSKMTVSRVINHPEQVRPELREMILNVMTQLDYHPNAAARALVGRRTR
CEEHHHHHHHCCCHHHHHHHHHCCHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEE
VVKLTIFEDMDTTEPYYMMLLAGIANELNQHQYALQIATRNSYEVGECDGYILTGIRQAD
EEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCEEEECCCHHH
YAWVERLNQPVVFVGSNRQGYDYVDTNNYQAVWESTRYAAEQGYTHLVFIGIAIAEPFEL
HHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHH
DRERGFREYVTTHHISAQIIRLANHSHVATAYVKSHWSGFPRNTAFICASDRLAIGVETA
HHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEE
IINQGGRVPIEYGIIGFDGVLLNQIAAKRLTTMKQPLEAMGRAAARLLLANINHTDRSRG
EECCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCC
RHDQEKIIINSRLSVAESTR
CCCCCEEEEECCCCHHCCCC
>Mature Secondary Structure
MITLNDVAKKANVSKMTVSRVINHPEQVRPELREMILNVMTQLDYHPNAAARALVGRRTR
CEEHHHHHHHCCCHHHHHHHHHCCHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEE
VVKLTIFEDMDTTEPYYMMLLAGIANELNQHQYALQIATRNSYEVGECDGYILTGIRQAD
EEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCEEEECCCHHH
YAWVERLNQPVVFVGSNRQGYDYVDTNNYQAVWESTRYAAEQGYTHLVFIGIAIAEPFEL
HHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHH
DRERGFREYVTTHHISAQIIRLANHSHVATAYVKSHWSGFPRNTAFICASDRLAIGVETA
HHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEE
IINQGGRVPIEYGIIGFDGVLLNQIAAKRLTTMKQPLEAMGRAAARLLLANINHTDRSRG
EECCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCC
RHDQEKIIINSRLSVAESTR
CCCCCEEEEECCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]