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The map label for this gene is thiD [H]

Identifier: 28377063

GI number: 28377063

Start: 100250

End: 101074

Strand: Direct

Name: thiD [H]

Synonym: lp_0114

Alternate gene names: 28377063

Gene position: 100250-101074 (Clockwise)

Preceding gene: 28377062

Following gene: 28377064

Centisome position: 3.03

GC content: 51.52

Gene sequence:

>825_bases
ATGAATGAATTTCCACAAGTGGCAACGATTGCTGGTACGGACAGTGGTGGCGGTGCCGGTGTCATGGCCGATTTAAAAAC
GATGCAGGCACGTCACGTTTTTGGTACCGCGGTAGTCGTTGCCGTGACAGCTCAAAATACATTGGGGGTCCAAGATTTTA
TGGCAATGCCGACGAAGCTCATTGACGAACAATTTGCTTCGTTAGCAGCTGACTTAGACATTCGCGCTTGTAAAACGGGG
ATGCTTGCGGATGCTGAACATGTCCATGCCGTTGTCGAGAACTTGAAACGAGTTGATTTTGGGCCGCTGACAGTTGATCC
AGTCATGATTGCGAAAGGCGGGGCGGCGTTATTAGCCGCGGATGCGATTAAAACGGTTCGCGACGAACTGCTCCCGTTAG
CGACTGTTGTGACGCCGAATTTGCCGGAAGCAGAACGATTAACCGGTCAGGCAATCACTAGTAATCAAGCGATGGTTACA
GCTGGGCATGCGCTACAGGGGTTAGGTGCTGACAATGTCATTATTAAAGGTGGGCATGGTGATAATCCTGATTTAGCTAA
TGACTTCGTATTGTTAGCTGACGGGACCGCCTTTTGGCTTTCGGCTCCACGGATTGATACGGTGCGGACCCACGGCACGG
GAGATACCCTGTCGGCATGTATTACCGCCGAATTAGCAAAAGGACGGTCAATGGCTGCGGCCATTAAAACGGCTAAGGCG
TACGTTGCTGGGACGATTCAGGATGGTATCCAGGTCGGGCATGGTCATGGCCCGTTGAATCATTGGGCAACCCCAAGTGA
GCAGGTGATCGTTCATGACGCTTAA

Upstream 100 bases:

>100_bases
TTTGCCATTGATTTGATTGACCGGCTGAGCTTAGTGACAGTGTCAGAAATTCAAACAATTGCTAAAGTTGATTAAATATA
ATGAAGGGTCGCGAGATTTA

Downstream 100 bases:

>100_bases
ATTTGAACCCACACAATTACGTGCTTATTTTGTGTGCGGGACACAAGACGTGCCGGGACAGGATCTAAATATGGTCGTCC
AAACGGCCTTGGATGCAGGC

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MNEFPQVATIAGTDSGGGAGVMADLKTMQARHVFGTAVVVAVTAQNTLGVQDFMAMPTKLIDEQFASLAADLDIRACKTG
MLADAEHVHAVVENLKRVDFGPLTVDPVMIAKGGAALLAADAIKTVRDELLPLATVVTPNLPEAERLTGQAITSNQAMVT
AGHALQGLGADNVIIKGGHGDNPDLANDFVLLADGTAFWLSAPRIDTVRTHGTGDTLSACITAELAKGRSMAAAIKTAKA
YVAGTIQDGIQVGHGHGPLNHWATPSEQVIVHDA

Sequences:

>Translated_274_residues
MNEFPQVATIAGTDSGGGAGVMADLKTMQARHVFGTAVVVAVTAQNTLGVQDFMAMPTKLIDEQFASLAADLDIRACKTG
MLADAEHVHAVVENLKRVDFGPLTVDPVMIAKGGAALLAADAIKTVRDELLPLATVVTPNLPEAERLTGQAITSNQAMVT
AGHALQGLGADNVIIKGGHGDNPDLANDFVLLADGTAFWLSAPRIDTVRTHGTGDTLSACITAELAKGRSMAAAIKTAKA
YVAGTIQDGIQVGHGHGPLNHWATPSEQVIVHDA
>Mature_274_residues
MNEFPQVATIAGTDSGGGAGVMADLKTMQARHVFGTAVVVAVTAQNTLGVQDFMAMPTKLIDEQFASLAADLDIRACKTG
MLADAEHVHAVVENLKRVDFGPLTVDPVMIAKGGAALLAADAIKTVRDELLPLATVVTPNLPEAERLTGQAITSNQAMVT
AGHALQGLGADNVIIKGGHGDNPDLANDFVLLADGTAFWLSAPRIDTVRTHGTGDTLSACITAELAKGRSMAAAIKTAKA
YVAGTIQDGIQVGHGHGPLNHWATPSEQVIVHDA

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=257, Percent_Identity=39.6887159533074, Blast_Score=174, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6325378, Length=272, Percent_Identity=28.3088235294118, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324517, Length=273, Percent_Identity=30.03663003663, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324997, Length=273, Percent_Identity=29.6703296703297, Blast_Score=102, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28325; Mature: 28325

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEFPQVATIAGTDSGGGAGVMADLKTMQARHVFGTAVVVAVTAQNTLGVQDFMAMPTKL
CCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCEEEEEEEECCCCCCHHHHHHHHHHH
IDEQFASLAADLDIRACKTGMLADAEHVHAVVENLKRVDFGPLTVDPVMIAKGGAALLAA
HHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEECCEEEECCCCHHHHH
DAIKTVRDELLPLATVVTPNLPEAERLTGQAITSNQAMVTAGHALQGLGADNVIIKGGHG
HHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEECCCEEEEECCHHHCCCCCCEEEECCCC
DNPDLANDFVLLADGTAFWLSAPRIDTVRTHGTGDTLSACITAELAKGRSMAAAIKTAKA
CCCCCCCCEEEEECCCEEEEECCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
YVAGTIQDGIQVGHGHGPLNHWATPSEQVIVHDA
HHEEEHHHCEEECCCCCCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MNEFPQVATIAGTDSGGGAGVMADLKTMQARHVFGTAVVVAVTAQNTLGVQDFMAMPTKL
CCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCEEEEEEEECCCCCCHHHHHHHHHHH
IDEQFASLAADLDIRACKTGMLADAEHVHAVVENLKRVDFGPLTVDPVMIAKGGAALLAA
HHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEECCEEEECCCCHHHHH
DAIKTVRDELLPLATVVTPNLPEAERLTGQAITSNQAMVTAGHALQGLGADNVIIKGGHG
HHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEECCCEEEEECCHHHCCCCCCEEEECCCC
DNPDLANDFVLLADGTAFWLSAPRIDTVRTHGTGDTLSACITAELAKGRSMAAAIKTAKA
CCCCCCCCEEEEECCCEEEEECCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
YVAGTIQDGIQVGHGHGPLNHWATPSEQVIVHDA
HHEEEHHHCEEECCCCCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]