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The map label for this gene is thiM

Identifier: 28377062

GI number: 28377062

Start: 99433

End: 100224

Strand: Direct

Name: thiM

Synonym: lp_0113

Alternate gene names: 28377062

Gene position: 99433-100224 (Clockwise)

Preceding gene: 28377060

Following gene: 28377063

Centisome position: 3.01

GC content: 49.12

Gene sequence:

>792_bases
ATGGAATTACAATTATTGAATACTTTACGGAATCACAATCCGATTGTTTTTAACATTGCCAATTTTGTTACGGTTCAAGA
CGTTGCCAATGGGCTAAACGCGTTAGGGGCCTCGCCAATTATGTCAGCTGAAGTCCAAGAAGCGGAGACGATGGTCCAAA
TTGCCGGGGCTGTTTGCATTAATTTAGGAACCCTGACCACCACCCAGATTGATCAAATGCGGGTGGTTGGTAAGCTTGCT
AATCAGTATCATAAGCCAGTCGTTCTTGATCCGGTGGCCGTCGGGGCGGTCCCTTATCGAAAAGAAGTCGCGCTAGCATT
ACTATCTGATTTTCAAGTCGATGTTATTCGGGGCAATGCTGGTGAAATTGCGGCATTGGCAGGCATGGATTGGCAAGCGA
AGGGAATTGATGCGGGGAGCGGTCAGGGCGACCTGGTTGAAATTGCTCAAGCTTGTGCACAGCAATTCCAATGCTGTGTC
ATATTGTCGGGACCTACTGATGTTATTACGGATGGTCAGCGCGTAGTTAAAGTCGCTAATGGTACCCCGCTCTTCCAACG
TCACGTGGGCTCGGGCGATTTACTTTCTAGTATCGTCGCGGCTTTTACAGCCGTTAGTCCAACCGACGTTTACCAGGCGG
CGCAAGTGGCTTGCTTAGTCTTAGCGGGAGCGGGGGAACTTGTCGCCAGTCAGCTGACGGCCGATCGTCCCGCCACATTT
GCCATTGATTTGATTGACCGGCTGAGCTTAGTGACAGTGTCAGAAATTCAAACAATTGCTAAAGTTGATTAA

Upstream 100 bases:

>100_bases
AGGAAAGTGTCACAGCTAATGTTAGCGGCCACTGGATCAATGCATGTTGATTGGTGGCCGCTTTTAATTGGCGTGACGGT
CAATGAGGAGGAGAATTGCT

Downstream 100 bases:

>100_bases
ATATAATGAAGGGTCGCGAGATTTAATGAATGAATTTCCACAAGTGGCAACGATTGCTGGTACGGACAGTGGTGGCGGTG
CCGGTGTCATGGCCGATTTA

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MELQLLNTLRNHNPIVFNIANFVTVQDVANGLNALGASPIMSAEVQEAETMVQIAGAVCINLGTLTTTQIDQMRVVGKLA
NQYHKPVVLDPVAVGAVPYRKEVALALLSDFQVDVIRGNAGEIAALAGMDWQAKGIDAGSGQGDLVEIAQACAQQFQCCV
ILSGPTDVITDGQRVVKVANGTPLFQRHVGSGDLLSSIVAAFTAVSPTDVYQAAQVACLVLAGAGELVASQLTADRPATF
AIDLIDRLSLVTVSEIQTIAKVD

Sequences:

>Translated_263_residues
MELQLLNTLRNHNPIVFNIANFVTVQDVANGLNALGASPIMSAEVQEAETMVQIAGAVCINLGTLTTTQIDQMRVVGKLA
NQYHKPVVLDPVAVGAVPYRKEVALALLSDFQVDVIRGNAGEIAALAGMDWQAKGIDAGSGQGDLVEIAQACAQQFQCCV
ILSGPTDVITDGQRVVKVANGTPLFQRHVGSGDLLSSIVAAFTAVSPTDVYQAAQVACLVLAGAGELVASQLTADRPATF
AIDLIDRLSLVTVSEIQTIAKVD
>Mature_263_residues
MELQLLNTLRNHNPIVFNIANFVTVQDVANGLNALGASPIMSAEVQEAETMVQIAGAVCINLGTLTTTQIDQMRVVGKLA
NQYHKPVVLDPVAVGAVPYRKEVALALLSDFQVDVIRGNAGEIAALAGMDWQAKGIDAGSGQGDLVEIAQACAQQFQCCV
ILSGPTDVITDGQRVVKVANGTPLFQRHVGSGDLLSSIVAAFTAVSPTDVYQAAQVACLVLAGAGELVASQLTADRPATF
AIDLIDRLSLVTVSEIQTIAKVD

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=252, Percent_Identity=37.6984126984127, Blast_Score=145, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6325042, Length=271, Percent_Identity=27.6752767527675, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_LACPL (Q890C2)

Other databases:

- EMBL:   AL935252
- RefSeq:   NP_783954.1
- ProteinModelPortal:   Q890C2
- SMR:   Q890C2
- GeneID:   1061358
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0113
- NMPDR:   fig|220668.1.peg.88
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- ProtClustDB:   PRK09355
- BioCyc:   LPLA220668:LP_0113-MONOMER
- BRENDA:   2.7.1.50
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 27521; Mature: 27521

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: NA

Important sites: BINDING 41-41 BINDING 117-117 BINDING 163-163 BINDING 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELQLLNTLRNHNPIVFNIANFVTVQDVANGLNALGASPIMSAEVQEAETMVQIAGAVCI
CCHHHHHHHHCCCCEEEEHHHHEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
NLGTLTTTQIDQMRVVGKLANQYHKPVVLDPVAVGAVPYRKEVALALLSDFQVDVIRGNA
CCCCCCHHHHHHHHHHHHHHHHHCCCEEECCHHHCCCCCHHHHHHHHHHCCEEEEEECCC
GEIAALAGMDWQAKGIDAGSGQGDLVEIAQACAQQFQCCVILSGPTDVITDGQRVVKVAN
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEEECC
GTPLFQRHVGSGDLLSSIVAAFTAVSPTDVYQAAQVACLVLAGAGELVASQLTADRPATF
CCCHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCHHHHHHHHCCCCCCCH
AIDLIDRLSLVTVSEIQTIAKVD
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MELQLLNTLRNHNPIVFNIANFVTVQDVANGLNALGASPIMSAEVQEAETMVQIAGAVCI
CCHHHHHHHHCCCCEEEEHHHHEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
NLGTLTTTQIDQMRVVGKLANQYHKPVVLDPVAVGAVPYRKEVALALLSDFQVDVIRGNA
CCCCCCHHHHHHHHHHHHHHHHHCCCEEECCHHHCCCCCHHHHHHHHHHCCEEEEEECCC
GEIAALAGMDWQAKGIDAGSGQGDLVEIAQACAQQFQCCVILSGPTDVITDGQRVVKVAN
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEEECC
GTPLFQRHVGSGDLLSSIVAAFTAVSPTDVYQAAQVACLVLAGAGELVASQLTADRPATF
CCCHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCHHHHHHHHCCCCCCCH
AIDLIDRLSLVTVSEIQTIAKVD
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566