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The map label for this gene is amy1 [H]

Identifier: 28376997

GI number: 28376997

Start: 29167

End: 30984

Strand: Direct

Name: amy1 [H]

Synonym: lp_0025

Alternate gene names: 28376997

Gene position: 29167-30984 (Clockwise)

Preceding gene: 28376996

Following gene: 28376998

Centisome position: 0.88

GC content: 49.17

Gene sequence:

>1818_bases
ATGCAGGTAACGTATAACTCATTTTTACCCACGTATAAGACACCCTTTGGTGCCGTGACCCGCCAGACACCCGTTCAATT
CACAATCAGTGTTCAGACAGTAACGCCGGTTAAGCAGATTGATTTATGCGTGGCTCATGATGGTCAGTGGGATGATGAAC
AGGCCTTACCGTTACATCCAATCAGTCCCACAATGTATACGGCCACGTTTATACCGACCCAGGTTGGCCTGTACTTCTAC
TATTTTCGGATTGAAACGGCGACCATGACCGCTTATTATGGTTGCGTTGATGGTGGTTATGGTGGCCCGGGCGTTCAATA
CGTCTTGCGCGAGCATGTTCAAATGTATCAATTGACAATCTTGAAAGCAGTAGAAACATTGCCACAATGGTATCGTGAAG
GGGTTGCTTATCAGATCTTTGTTGACCGCTTCAATAATGGCAACGCGGATGGCCACGTTAATGCGCCCAAGCCAAATAGC
TTTATTTATGGTCAACTAACGGATCGCCCACTATACGTGCGTGATGAACACCGGGCGATTTTACGCTGGGATTTTTACGG
TGGTAATTTGCGCGGTATTAGTGCTAAGATTCCATATTTACAGCGACTCGGTGTGACAATTATTTATCTGACGCCGATCT
TTGAGGCCAGCAGTAATCATCGGTATGATACGGGTGACTATCTGAAGATTGATCCGGTCGTCGGCGATTTGACGGACTTT
GATAACCTGGTCGCGGCCGTACACCGGGCCGGTATGCGACTGATCTTAGACGGTGTTTTTAACCATGTTGGTGTGGATTC
TAAGTATTTCAATCAGGCGGGCCATTACCCGACGGTTGGGGCAACTCAGTCGACCACGAGTCCGTACTACCCGTGGTTCA
CATTCATTCATTATCCCCACGATTACGATAGCTGGTGGGGCATCCGTGATCTGCCGACAGTTGATAAAGACGATCCAACA
TACCGCGATTTTATCTTTGGTAAACCCGATTCGGTCATTGACTATTGGACGCAACGCGGTGTGGATGGCTGGCGCCTAGA
TGTCGCGGATGAGTTGCCAGACGATTTCATTGCTGGAATTCGACGGACCCTTGACCGTTATCCGGATAAGGTTTTGATCG
GTGAAGTTTGGGAGGATGCCTCTCATAAATTAGCGTATGGGATGCGCCGGCACTACTTAGAGGGCGGCGGCTTACAGGCT
GTCATGAATTATCCGCTCCGCCGCTTGATTATTCACGTTTTAAACGGCACGCTAACACCGGCCGAATGGTGGCGTGAACT
AATGACCCTCAAGGAAAATTATCCCGCTACGACGTTTCGCTTTAACTTCAATAACATCGGTAGCCATGATACGCCCAGAA
TCCTGACTATGTTAGCCGAAGATCGCCAACGCCTACGTTTGGCCGTCCAACTACTCTTGACGCTTCCAGGTGTACCGTGC
CTGTATTACGGTGATGAGGCGTTGATGATGGGGGGGAAAGACCCTGATAACCGTGCGTTTTATCCGTGGCAGGACGCAGA
TCAGGCGCAAGTTCAGTTTGTCGCTGACTGGGCGAATTGGCGTCAACGGCACCCGTGGTTAAACACAGCCCACTTTGTGC
CGTTTTACTTGGCAGATTATGGCATTGGCTACCTTTATTGGCAGGCTGATCAGACCGTGATGGTGATTGTCAATGTGACT
ACTGAGCCGCGAACCATGACGAGTCACGATTTAAAGTTGAGTGGTGTGCCCATAAAATTAGCACAAGCGATTCAATGCTG
CCTTGTGACACAGACGCTGGGTGGTGAACAGTTGCGCCTTATTGAAAACTTTACCTAA

Upstream 100 bases:

>100_bases
CTTCGGGACACTTTGCCGCGGACTTAGCAGTGGCGCGGTATGCTGACGATATTTGGCAAGTGCCCCACGAACGACTGAAT
TAAGAAAGGAAGGGTGACGG

Downstream 100 bases:

>100_bases
ATTTTACAGGAACTTGATGATTGTTAGTACGGTTCAGCCGTATAGTATAATTGTACTTTTTAATAGCACCCCGCTATTGA
TTATGTATTACGAAGTACTC

Product: alpha-amylase

Products: NA

Alternate protein names: Alpha-amylase/pullulanase; Alpha-amylase; 1,4-alpha-D-glucan glucanohydrolase; Pullulanase; 1,4-alpha-D-glucan glucanohydrolase; Alpha-dextrin endo-1,6-alpha-glucosidase [H]

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MQVTYNSFLPTYKTPFGAVTRQTPVQFTISVQTVTPVKQIDLCVAHDGQWDDEQALPLHPISPTMYTATFIPTQVGLYFY
YFRIETATMTAYYGCVDGGYGGPGVQYVLREHVQMYQLTILKAVETLPQWYREGVAYQIFVDRFNNGNADGHVNAPKPNS
FIYGQLTDRPLYVRDEHRAILRWDFYGGNLRGISAKIPYLQRLGVTIIYLTPIFEASSNHRYDTGDYLKIDPVVGDLTDF
DNLVAAVHRAGMRLILDGVFNHVGVDSKYFNQAGHYPTVGATQSTTSPYYPWFTFIHYPHDYDSWWGIRDLPTVDKDDPT
YRDFIFGKPDSVIDYWTQRGVDGWRLDVADELPDDFIAGIRRTLDRYPDKVLIGEVWEDASHKLAYGMRRHYLEGGGLQA
VMNYPLRRLIIHVLNGTLTPAEWWRELMTLKENYPATTFRFNFNNIGSHDTPRILTMLAEDRQRLRLAVQLLLTLPGVPC
LYYGDEALMMGGKDPDNRAFYPWQDADQAQVQFVADWANWRQRHPWLNTAHFVPFYLADYGIGYLYWQADQTVMVIVNVT
TEPRTMTSHDLKLSGVPIKLAQAIQCCLVTQTLGGEQLRLIENFT

Sequences:

>Translated_605_residues
MQVTYNSFLPTYKTPFGAVTRQTPVQFTISVQTVTPVKQIDLCVAHDGQWDDEQALPLHPISPTMYTATFIPTQVGLYFY
YFRIETATMTAYYGCVDGGYGGPGVQYVLREHVQMYQLTILKAVETLPQWYREGVAYQIFVDRFNNGNADGHVNAPKPNS
FIYGQLTDRPLYVRDEHRAILRWDFYGGNLRGISAKIPYLQRLGVTIIYLTPIFEASSNHRYDTGDYLKIDPVVGDLTDF
DNLVAAVHRAGMRLILDGVFNHVGVDSKYFNQAGHYPTVGATQSTTSPYYPWFTFIHYPHDYDSWWGIRDLPTVDKDDPT
YRDFIFGKPDSVIDYWTQRGVDGWRLDVADELPDDFIAGIRRTLDRYPDKVLIGEVWEDASHKLAYGMRRHYLEGGGLQA
VMNYPLRRLIIHVLNGTLTPAEWWRELMTLKENYPATTFRFNFNNIGSHDTPRILTMLAEDRQRLRLAVQLLLTLPGVPC
LYYGDEALMMGGKDPDNRAFYPWQDADQAQVQFVADWANWRQRHPWLNTAHFVPFYLADYGIGYLYWQADQTVMVIVNVT
TEPRTMTSHDLKLSGVPIKLAQAIQCCLVTQTLGGEQLRLIENFT
>Mature_605_residues
MQVTYNSFLPTYKTPFGAVTRQTPVQFTISVQTVTPVKQIDLCVAHDGQWDDEQALPLHPISPTMYTATFIPTQVGLYFY
YFRIETATMTAYYGCVDGGYGGPGVQYVLREHVQMYQLTILKAVETLPQWYREGVAYQIFVDRFNNGNADGHVNAPKPNS
FIYGQLTDRPLYVRDEHRAILRWDFYGGNLRGISAKIPYLQRLGVTIIYLTPIFEASSNHRYDTGDYLKIDPVVGDLTDF
DNLVAAVHRAGMRLILDGVFNHVGVDSKYFNQAGHYPTVGATQSTTSPYYPWFTFIHYPHDYDSWWGIRDLPTVDKDDPT
YRDFIFGKPDSVIDYWTQRGVDGWRLDVADELPDDFIAGIRRTLDRYPDKVLIGEVWEDASHKLAYGMRRHYLEGGGLQA
VMNYPLRRLIIHVLNGTLTPAEWWRELMTLKENYPATTFRFNFNNIGSHDTPRILTMLAEDRQRLRLAVQLLLTLPGVPC
LYYGDEALMMGGKDPDNRAFYPWQDADQAQVQFVADWANWRQRHPWLNTAHFVPFYLADYGIGYLYWQADQTVMVIVNVT
TEPRTMTSHDLKLSGVPIKLAQAIQCCLVTQTLGGEQLRLIENFT

Specific function: May Play A Role In Regulating The Intracellular Level Of Maltotriose. Cleaves Glucose From The Reducing End Of Maltotriose And Longer Maltodextrins With A Chain Length Of Up To 7 Glucose Units. [C]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 fibronectin type-III domains [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=423, Percent_Identity=22.4586288416076, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI1786604, Length=412, Percent_Identity=34.2233009708738, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI1789995, Length=552, Percent_Identity=24.2753623188406, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1790687, Length=417, Percent_Identity=24.4604316546763, Blast_Score=93, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324416, Length=261, Percent_Identity=26.4367816091954, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322241, Length=261, Percent_Identity=26.4367816091954, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322021, Length=261, Percent_Identity=26.4367816091954, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321726, Length=253, Percent_Identity=26.0869565217391, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321731, Length=447, Percent_Identity=23.489932885906, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6319776, Length=447, Percent_Identity=23.489932885906, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6322245, Length=250, Percent_Identity=24.4, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24586591, Length=479, Percent_Identity=24.6346555323591, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24583745, Length=439, Percent_Identity=23.4624145785877, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24586587, Length=373, Percent_Identity=23.5924932975871, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI221330053, Length=213, Percent_Identity=29.5774647887324, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24586599, Length=377, Percent_Identity=23.8726790450928, Blast_Score=76, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24583749, Length=372, Percent_Identity=22.8494623655914, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24583747, Length=372, Percent_Identity=22.8494623655914, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24586589, Length=367, Percent_Identity=23.7057220708447, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24586597, Length=188, Percent_Identity=27.6595744680851, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013784
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR002044
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N; PF00686 CBM_20; PF00041 fn3 [H]

EC number: =3.2.1.1; =3.2.1.41 [H]

Molecular weight: Translated: 69506; Mature: 69506

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVTYNSFLPTYKTPFGAVTRQTPVQFTISVQTVTPVKQIDLCVAHDGQWDDEQALPLHP
CEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEECCHHHEEEEEECCCCCCCCCCCCCCC
ISPTMYTATFIPTQVGLYFYYFRIETATMTAYYGCVDGGYGGPGVQYVLREHVQMYQLTI
CCCCEEEEEECCHHHCEEEEEEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
LKAVETLPQWYREGVAYQIFVDRFNNGNADGHVNAPKPNSFIYGQLTDRPLYVRDEHRAI
HHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEECCCCCCCEEEEEECCCEEEEECCCCEE
LRWDFYGGNLRGISAKIPYLQRLGVTIIYLTPIFEASSNHRYDTGDYLKIDPVVGDLTDF
EEEEEECCCEEEEEECCCHHHHCCEEEEEEEEEEECCCCCCCCCCCEEEECCCCCCHHHH
DNLVAAVHRAGMRLILDGVFNHVGVDSKYFNQAGHYPTVGATQSTTSPYYPWFTFIHYPH
HHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCC
DYDSWWGIRDLPTVDKDDPTYRDFIFGKPDSVIDYWTQRGVDGWRLDVADELPDDFIAGI
CCCCCCCCCCCCCCCCCCCCHHHEECCCCCHHHHHHHHCCCCCEEECCHHHCCHHHHHHH
RRTLDRYPDKVLIGEVWEDASHKLAYGMRRHYLEGGGLQAVMNYPLRRLIIHVLNGTLTP
HHHHHHCCCEEEEHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHCCCCCH
AEWWRELMTLKENYPATTFRFNFNNIGSHDTPRILTMLAEDRQRLRLAVQLLLTLPGVPC
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LYYGDEALMMGGKDPDNRAFYPWQDADQAQVQFVADWANWRQRHPWLNTAHFVPFYLADY
EEECCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCCCCHHHHHHHHC
GIGYLYWQADQTVMVIVNVTTEPRTMTSHDLKLSGVPIKLAQAIQCCLVTQTLGGEQLRL
CCEEEEEECCCEEEEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCEEEE
IENFT
EECCC
>Mature Secondary Structure
MQVTYNSFLPTYKTPFGAVTRQTPVQFTISVQTVTPVKQIDLCVAHDGQWDDEQALPLHP
CEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEECCHHHEEEEEECCCCCCCCCCCCCCC
ISPTMYTATFIPTQVGLYFYYFRIETATMTAYYGCVDGGYGGPGVQYVLREHVQMYQLTI
CCCCEEEEEECCHHHCEEEEEEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
LKAVETLPQWYREGVAYQIFVDRFNNGNADGHVNAPKPNSFIYGQLTDRPLYVRDEHRAI
HHHHHHHHHHHHCCCEEEEEEEECCCCCCCCEECCCCCCCEEEEEECCCEEEEECCCCEE
LRWDFYGGNLRGISAKIPYLQRLGVTIIYLTPIFEASSNHRYDTGDYLKIDPVVGDLTDF
EEEEEECCCEEEEEECCCHHHHCCEEEEEEEEEEECCCCCCCCCCCEEEECCCCCCHHHH
DNLVAAVHRAGMRLILDGVFNHVGVDSKYFNQAGHYPTVGATQSTTSPYYPWFTFIHYPH
HHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCC
DYDSWWGIRDLPTVDKDDPTYRDFIFGKPDSVIDYWTQRGVDGWRLDVADELPDDFIAGI
CCCCCCCCCCCCCCCCCCCCHHHEECCCCCHHHHHHHHCCCCCEEECCHHHCCHHHHHHH
RRTLDRYPDKVLIGEVWEDASHKLAYGMRRHYLEGGGLQAVMNYPLRRLIIHVLNGTLTP
HHHHHHCCCEEEEHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHCCCCCH
AEWWRELMTLKENYPATTFRFNFNNIGSHDTPRILTMLAEDRQRLRLAVQLLLTLPGVPC
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LYYGDEALMMGGKDPDNRAFYPWQDADQAQVQFVADWANWRQRHPWLNTAHFVPFYLADY
EEECCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCCCCHHHHHHHHC
GIGYLYWQADQTVMVIVNVTTEPRTMTSHDLKLSGVPIKLAQAIQCCLVTQTLGGEQLRL
CCEEEEEECCCEEEEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCEEEE
IENFT
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2391488; 3260488; 1409594 [H]