Click here to switch to the map view.

The map label for this gene is dnaN [H]

Identifier: 28376976

GI number: 28376976

Start: 1546

End: 2685

Strand: Direct

Name: dnaN [H]

Synonym: lp_0002

Alternate gene names: 28376976

Gene position: 1546-2685 (Clockwise)

Preceding gene: 28376975

Following gene: 28376977

Centisome position: 0.05

GC content: 42.54

Gene sequence:

>1140_bases
ATGAAATTCACGATCAATCGATCTGCCTTCATCAAAGAATTAAATAACGTCCAACGGGCCATTTCTTCAAAAACGACCAT
TCCAATTTTGACTGGTCTTAAACTTGACGTTAATACTGACGCTATTACCTTAACTGGTAGTGATGCTGATATTTCTATTG
AAACCACCATTCCAGCAAGTGATGACAACAATACGTTAGTCGTTGAAGATGCCGGTTCAATCGTTTTACCTGCCCGTTTC
TTTAGTGAAATCGTAAAAAAATTACCAGAAGACACCATGACTGTTAACGTGGTCGACGGTTTTCAAACACAAATTACTTC
AGGGGCCGCTTCCTTTACAATCAACGGCTTAGACCCTGAAAACTACCCACACTTACCTGAAATTGATACGACTAATACCA
TCACGTTAGCTGGGGATGTACTCAAAGAGTTGATTGGTCAGACCGTTATTGCCGTTTCCAATCAGGAAAGTCGACCAATT
CTGACCGGGGTTCATTTTATCTTAGCTAACGGTGAGTTCTTAGCGGTCGCAACCGATTCACACCGCCTAAGTCAACGCCG
AATCAAGTTGCCAGAAGCCAATAATGCCAATTATGATGTAATCATTCCTGGTAAAAGTTTGACAGAATTATCTCGGATGA
TCGGCGACAATAATCCTGACGTGCAGATGCGGTTATCAGAAAACCAAGTCCTATTTGTGCTGGGGAATACGTCCTTCTAT
TCTCGCTTACTCGAAGGAAATTATCCCGACACGTCACGGCTGATTCCTAAAGAATCTAATACGACTGTTGAAATTTCAGC
ACCCGCACTTTCAGCAGCAATTGAACGGGCATCCCTGTTATCCCACGAAAGTCGCAATAATGTGGTTCGCTTCTCCGTTA
ACCCAACGGACAAAACGATTACGATTTTCGGTAACTCACCAGATGTCGGGGAAGTAACGGAACAACTACAGCCCACTGAT
TTGTCAGGTGACGAACTTGAAATTTCATTTAACCCAGACTACATGAAAGAAGCGTTACGTTCGTTTGGGCAAGCTATGAT
CAAAATCTCATTCACGATGGCATTGCGCCCATTCACTTTAGTGCCAACTGAAGAAGGCGAAAACTTTATTCAGTTGATTA
CACCAGTTCGAACGTTTTAA

Upstream 100 bases:

>100_bases
ACTCGGTTTTCCACAGTACCCACTGCTCTACTATTACTACGAACTTTTATTATTAATATATATCTAGTGCCCGTCGCTAG
AATAAAGAGGGGATTAACTT

Downstream 100 bases:

>100_bases
TTAGCTGAATAAATATACAATTCTAAATAAAAGGCCCGCATTTCACCGTGAAATGCGGGCCTTTTATGACGGCTAAAACT
AGCTAAAGATGGGGCAATGA

Product: DNA-directed DNA polymerase III, beta chain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKFTINRSAFIKELNNVQRAISSKTTIPILTGLKLDVNTDAITLTGSDADISIETTIPASDDNNTLVVEDAGSIVLPARF
FSEIVKKLPEDTMTVNVVDGFQTQITSGAASFTINGLDPENYPHLPEIDTTNTITLAGDVLKELIGQTVIAVSNQESRPI
LTGVHFILANGEFLAVATDSHRLSQRRIKLPEANNANYDVIIPGKSLTELSRMIGDNNPDVQMRLSENQVLFVLGNTSFY
SRLLEGNYPDTSRLIPKESNTTVEISAPALSAAIERASLLSHESRNNVVRFSVNPTDKTITIFGNSPDVGEVTEQLQPTD
LSGDELEISFNPDYMKEALRSFGQAMIKISFTMALRPFTLVPTEEGENFIQLITPVRTF

Sequences:

>Translated_379_residues
MKFTINRSAFIKELNNVQRAISSKTTIPILTGLKLDVNTDAITLTGSDADISIETTIPASDDNNTLVVEDAGSIVLPARF
FSEIVKKLPEDTMTVNVVDGFQTQITSGAASFTINGLDPENYPHLPEIDTTNTITLAGDVLKELIGQTVIAVSNQESRPI
LTGVHFILANGEFLAVATDSHRLSQRRIKLPEANNANYDVIIPGKSLTELSRMIGDNNPDVQMRLSENQVLFVLGNTSFY
SRLLEGNYPDTSRLIPKESNTTVEISAPALSAAIERASLLSHESRNNVVRFSVNPTDKTITIFGNSPDVGEVTEQLQPTD
LSGDELEISFNPDYMKEALRSFGQAMIKISFTMALRPFTLVPTEEGENFIQLITPVRTF
>Mature_379_residues
MKFTINRSAFIKELNNVQRAISSKTTIPILTGLKLDVNTDAITLTGSDADISIETTIPASDDNNTLVVEDAGSIVLPARF
FSEIVKKLPEDTMTVNVVDGFQTQITSGAASFTINGLDPENYPHLPEIDTTNTITLAGDVLKELIGQTVIAVSNQESRPI
LTGVHFILANGEFLAVATDSHRLSQRRIKLPEANNANYDVIIPGKSLTELSRMIGDNNPDVQMRLSENQVLFVLGNTSFY
SRLLEGNYPDTSRLIPKESNTTVEISAPALSAAIERASLLSHESRNNVVRFSVNPTDKTITIFGNSPDVGEVTEQLQPTD
LSGDELEISFNPDYMKEALRSFGQAMIKISFTMALRPFTLVPTEEGENFIQLITPVRTF

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=353, Percent_Identity=29.4617563739377, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41521; Mature: 41521

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTINRSAFIKELNNVQRAISSKTTIPILTGLKLDVNTDAITLTGSDADISIETTIPAS
CEEEECHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCEEEEECCCCCEEEEEEECCC
DDNNTLVVEDAGSIVLPARFFSEIVKKLPEDTMTVNVVDGFQTQITSGAASFTINGLDPE
CCCCEEEEECCCCEEECHHHHHHHHHHCCCCCEEEEEECCCHHHHCCCCCEEEEECCCCC
NYPHLPEIDTTNTITLAGDVLKELIGQTVIAVSNQESRPILTGVHFILANGEFLAVATDS
CCCCCCCCCCCCEEEEHHHHHHHHHCCEEEEECCCCCCCEEEEEEEEEECCCEEEEEECC
HRLSQRRIKLPEANNANYDVIIPGKSLTELSRMIGDNNPDVQMRLSENQVLFVLGNTSFY
HHHHHHEEECCCCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEEECCCEEEEEEECHHHH
SRLLEGNYPDTSRLIPKESNTTVEISAPALSAAIERASLLSHESRNNVVRFSVNPTDKTI
HHHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEE
TIFGNSPDVGEVTEQLQPTDLSGDELEISFNPDYMKEALRSFGQAMIKISFTMALRPFTL
EEECCCCCHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHEEEEEEEEEEECCEEE
VPTEEGENFIQLITPVRTF
EECCCCCCEEEEECHHCCC
>Mature Secondary Structure
MKFTINRSAFIKELNNVQRAISSKTTIPILTGLKLDVNTDAITLTGSDADISIETTIPAS
CEEEECHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCEEEEECCCCCEEEEEEECCC
DDNNTLVVEDAGSIVLPARFFSEIVKKLPEDTMTVNVVDGFQTQITSGAASFTINGLDPE
CCCCEEEEECCCCEEECHHHHHHHHHHCCCCCEEEEEECCCHHHHCCCCCEEEEECCCCC
NYPHLPEIDTTNTITLAGDVLKELIGQTVIAVSNQESRPILTGVHFILANGEFLAVATDS
CCCCCCCCCCCCEEEEHHHHHHHHHCCEEEEECCCCCCCEEEEEEEEEECCCEEEEEECC
HRLSQRRIKLPEANNANYDVIIPGKSLTELSRMIGDNNPDVQMRLSENQVLFVLGNTSFY
HHHHHHEEECCCCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEEECCCEEEEEEECHHHH
SRLLEGNYPDTSRLIPKESNTTVEISAPALSAAIERASLLSHESRNNVVRFSVNPTDKTI
HHHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEE
TIFGNSPDVGEVTEQLQPTDLSGDELEISFNPDYMKEALRSFGQAMIKISFTMALRPFTL
EEECCCCCHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHEEEEEEEEEEECCEEE
VPTEEGENFIQLITPVRTF
EECCCCCCEEEEECHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377; 2846289 [H]