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The map label for this gene is ybbD [H]

Identifier: 28211770

GI number: 28211770

Start: 2287150

End: 2288367

Strand: Direct

Name: ybbD [H]

Synonym: CTC02158

Alternate gene names: 28211770

Gene position: 2287150-2288367 (Clockwise)

Preceding gene: 28211764

Following gene: 28211771

Centisome position: 81.71

GC content: 26.03

Gene sequence:

>1218_bases
TTGAAAAAATTTAATTTATTTATAGTTTTATTATTAATAAATTTTGCATTTTTACTAGGCTGTGGTAAAAATAATAACTC
TAATAATAGAAATATTAATAATGAAACTTCCAAAGCTCCAAAACTAAATAATGATAACTCTATGCAAGTTAAACTTAATA
GTATGAGCTTAGATGAGAAAATTGGTCAACTGCTTATTGTAGGTTTTGATGGATATGAAGCAAATAAGAATATAGAAAGT
TTAATTAAAAAAAATTACGTTGGTGGTGTTATTTTATTTGGAAACAATATAAGAAGTGCAGAACAAACAATGAAATTAAC
TAATTCATTAAAATCTATTAATTCAAAGAATAAAATTCCATTATTTATATCTGTTGATGAAGAAGGTGGACGGGTTAGCA
GAATGCCTAAAGAATTAGAAAAACTTCCTAGTAATAAGATTATAGGTGAAATAAATAATAGTAACCTTAGTTATAATATA
GGCAAAATAATTTCAAAAGAACTTACATGCCTTGGATTTAATATGAATTTTGCACCGATTCTAGATATAAATAGTAACCT
GCAAAATCCTGTAATTGGGGATAGATCCTTTGGTAATAATCCAAATTTAGTTACTAGACTTGGTATTAAAACTATGGAAG
GATTAAGAGAGGGTAATATTATTCCTGTTATAAAACATTTTCCAGGACATGGTGATACCTCAGTGGATTCTCATGTGGGA
CTTCCTTTAATTAATCATGATATGAAAAGATTAAAGGAGTTCGAATTAATCCCCTTTAAAGAGGCGATAAATAATAATGC
AGATGTAGTTATGATATCTCATATATTGTTACCTAAAATAGACTCCTCCTATCCTGCAACTATGTCAAAAATCATAATAA
AAGATGTTTTACGAAATGATTTAAAGTTTAATGGAGTGGTTATTACAGATGATATGACCATGAGTGCCATTACAAAAAAT
TTTGATATTTCTAATGCAGCTATTAAATCTATAAATGCTGGAACGGATATTATTTTAATATGTCATGGCTATGATAATGA
AATATACGTAATTAATTCTATAAAAGAAGCAGTAGAAAACAATATAATAACGGAAGATAAAATTAATGAAAGTGTTTATA
GAATACTAAAATTAAAGGAAAAGTATAAAATAAGTAACGATACAGTGGATAATATAGATGTTGACAAAATAAATAGTGAA
ATTAAAAGTATTTTTTAA

Upstream 100 bases:

>100_bases
TAAAATATATTTCATCTTTTATGACATATTGCTTTTATATAAGGCAATAATTGGATATAATTAAACAATATCAGTACCTT
TTTGGGAAAGGAAGATTTAA

Downstream 100 bases:

>100_bases
TATAAACTCTTTTCATATATTTTTATCCTATGGTAAAATCTTTATATAGAAAGGTTGTGATACTTTGAATATTGACTTTG
AAAAAATAGTAAATGAAGTT

Product: putative anhydromuramoyl-peptide exo-beta-N-acetylglucosaminidase

Products: N-acetylglucosamin; anhydro-N-acetylmuramic acid [C]

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MKKFNLFIVLLLINFAFLLGCGKNNNSNNRNINNETSKAPKLNNDNSMQVKLNSMSLDEKIGQLLIVGFDGYEANKNIES
LIKKNYVGGVILFGNNIRSAEQTMKLTNSLKSINSKNKIPLFISVDEEGGRVSRMPKELEKLPSNKIIGEINNSNLSYNI
GKIISKELTCLGFNMNFAPILDINSNLQNPVIGDRSFGNNPNLVTRLGIKTMEGLREGNIIPVIKHFPGHGDTSVDSHVG
LPLINHDMKRLKEFELIPFKEAINNNADVVMISHILLPKIDSSYPATMSKIIIKDVLRNDLKFNGVVITDDMTMSAITKN
FDISNAAIKSINAGTDIILICHGYDNEIYVINSIKEAVENNIITEDKINESVYRILKLKEKYKISNDTVDNIDVDKINSE
IKSIF

Sequences:

>Translated_405_residues
MKKFNLFIVLLLINFAFLLGCGKNNNSNNRNINNETSKAPKLNNDNSMQVKLNSMSLDEKIGQLLIVGFDGYEANKNIES
LIKKNYVGGVILFGNNIRSAEQTMKLTNSLKSINSKNKIPLFISVDEEGGRVSRMPKELEKLPSNKIIGEINNSNLSYNI
GKIISKELTCLGFNMNFAPILDINSNLQNPVIGDRSFGNNPNLVTRLGIKTMEGLREGNIIPVIKHFPGHGDTSVDSHVG
LPLINHDMKRLKEFELIPFKEAINNNADVVMISHILLPKIDSSYPATMSKIIIKDVLRNDLKFNGVVITDDMTMSAITKN
FDISNAAIKSINAGTDIILICHGYDNEIYVINSIKEAVENNIITEDKINESVYRILKLKEKYKISNDTVDNIDVDKINSE
IKSIF
>Mature_405_residues
MKKFNLFIVLLLINFAFLLGCGKNNNSNNRNINNETSKAPKLNNDNSMQVKLNSMSLDEKIGQLLIVGFDGYEANKNIES
LIKKNYVGGVILFGNNIRSAEQTMKLTNSLKSINSKNKIPLFISVDEEGGRVSRMPKELEKLPSNKIIGEINNSNLSYNI
GKIISKELTCLGFNMNFAPILDINSNLQNPVIGDRSFGNNPNLVTRLGIKTMEGLREGNIIPVIKHFPGHGDTSVDSHVG
LPLINHDMKRLKEFELIPFKEAINNNADVVMISHILLPKIDSSYPATMSKIIIKDVLRNDLKFNGVVITDDMTMSAITKN
FDISNAAIKSINAGTDIILICHGYDNEIYVINSIKEAVENNIITEDKINESVYRILKLKEKYKISNDTVDNIDVDKINSE
IKSIF

Specific function: Hydrolyses the beta-1,4-glycosidic bond between N-acetylglucosamine and anhydro-N-acetylmuramic acid in cell wall degradation products following their importation into the cell during the process for recycling cell wall muropeptides [C]

COG id: COG1472

COG function: function code G; Beta-glucosidase-related glycosidases

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787350, Length=290, Percent_Identity=27.5862068965517, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1788453, Length=357, Percent_Identity=22.96918767507, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019800
- InterPro:   IPR002772
- InterPro:   IPR001764
- InterPro:   IPR017853 [H]

Pfam domain/function: PF00933 Glyco_hydro_3; PF01915 Glyco_hydro_3_C [H]

EC number: NA

Molecular weight: Translated: 45231; Mature: 45231

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFNLFIVLLLINFAFLLGCGKNNNSNNRNINNETSKAPKLNNDNSMQVKLNSMSLDEK
CCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHC
IGQLLIVGFDGYEANKNIESLIKKNYVGGVILFGNNIRSAEQTMKLTNSLKSINSKNKIP
CCEEEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCE
LFISVDEEGGRVSRMPKELEKLPSNKIIGEINNSNLSYNIGKIISKELTCLGFNMNFAPI
EEEEECCCCCCHHHCHHHHHHCCCCCEEEEECCCCCEEEHHHHHHHCCEEEEECCCCEEE
LDINSNLQNPVIGDRSFGNNPNLVTRLGIKTMEGLREGNIIPVIKHFPGHGDTSVDSHVG
EECCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
LPLINHDMKRLKEFELIPFKEAINNNADVVMISHILLPKIDSSYPATMSKIIIKDVLRND
CCHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEEEEHHCCCCCCCCHHHHHHHHHHHHHCC
LKFNGVVITDDMTMSAITKNFDISNAAIKSINAGTDIILICHGYDNEIYVINSIKEAVEN
CEECCEEEECCCCHHHHHCCCCCCHHHHHHCCCCCCEEEEEECCCCCEEEEHHHHHHHHC
NIITEDKINESVYRILKLKEKYKISNDTVDNIDVDKINSEIKSIF
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKKFNLFIVLLLINFAFLLGCGKNNNSNNRNINNETSKAPKLNNDNSMQVKLNSMSLDEK
CCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHC
IGQLLIVGFDGYEANKNIESLIKKNYVGGVILFGNNIRSAEQTMKLTNSLKSINSKNKIP
CCEEEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCE
LFISVDEEGGRVSRMPKELEKLPSNKIIGEINNSNLSYNIGKIISKELTCLGFNMNFAPI
EEEEECCCCCCHHHCHHHHHHCCCCCEEEEECCCCCEEEHHHHHHHCCEEEEECCCCEEE
LDINSNLQNPVIGDRSFGNNPNLVTRLGIKTMEGLREGNIIPVIKHFPGHGDTSVDSHVG
EECCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
LPLINHDMKRLKEFELIPFKEAINNNADVVMISHILLPKIDSSYPATMSKIIIKDVLRND
CCHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEEEEHHCCCCCCCCHHHHHHHHHHHHHCC
LKFNGVVITDDMTMSAITKNFDISNAAIKSINAGTDIILICHGYDNEIYVINSIKEAVEN
CEECCEEEECCCCHHHHHCCCCCCHHHHHHCCCCCCEEEEEECCCCCEEEEHHHHHHHHC
NIITEDKINESVYRILKLKEKYKISNDTVDNIDVDKINSEIKSIF
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cell Wall [C]

Specific reaction: Cell Wall = N-acetylglucosamin + anhydro-N-acetylmuramic acid [C]

General reaction: Hydrolyse [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8011666; 9274029; 9384377 [H]