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The map label for this gene is pnp

Identifier: 28210966

GI number: 28210966

Start: 1372995

End: 1375112

Strand: Direct

Name: pnp

Synonym: CTC01280

Alternate gene names: 28210966

Gene position: 1372995-1375112 (Clockwise)

Preceding gene: 341904758

Following gene: 28210967

Centisome position: 49.05

GC content: 32.67

Gene sequence:

>2118_bases
ATGAGTCACGTACTTGAAACTGATATAGCAGGAAGAACCCTTAAAGTAGAATATGGAAAAGTAGGAATGCTTTCCAATTG
TGCTATATTAGTAAGCTATGGAGATACAGTGGTACTTATAAATGCAAATGCGTCTAAAGAACCAAGAGATGGAATAGATT
TCTTTCCATTAAGTGTAGAATATGAGGAGAGACTTTATTCTGTTGGAAAAATACCTGGAGGATTTATAAAAAGAGAGGGA
AGACCTACAGAGAATGCTATATTACATGCTAGAGCTATAGATAGACCCCTAAGACCTCTATTTCCAAAAGGATATAGAAA
TGATGTTCAAATAGTATGCACTGTCCTATCTGTAGAATCTGATAATCAACCAGATATATTAGCTATGAATGGAGCATCTT
TAGCTCTATGTCTATCTAGCATACCTTTTACGACTCCAGTAGCTAATGTTTCCGTAGGGCTAATTAATGGAGACTTTATA
TTAAATCCAACTCAAAGTCAAAGAGAAGAAAGTATCTTAAATTTAACTGTTTCAGCTACAAAGGAAAGAGTTATGATGAT
TGAAGCTGGTGCTGAAGAAGTAGATGAAGATACTATGTATAATGCAATAAGATTTGGTTTCGAACAATGTCAAAAAATAG
TGGAATTCCAAGAAGAGGCAATGAGAAGATTTGGGAAAGAAAAAGTAGAACCAGTTTTATACTTAGTGGATAAAGATATA
GAAAAAGAAGTAAGAGAATTCGCATTTCCTATGGTTAAAGAAGCTATGTACATTTCAGATAAAGACCTTAGAAATGAAAA
AATAGATGAAGTAAAAGAAAAAGTGAAAGAGGAGTTTGAAGAAAAGTATCCAGAGAATGATTCAGATATTTCAGAAGCTT
TATATAAAATGCAAAAAGAAGTAGTAAGAAATATGATACTTAGAGAAAAAAGACGTCCAGATGAAAGGGCTTTTGACGAA
ATAAGAAAAATAAGCTGTGAAGTAGGATTACTTCCAAGAACTCATGGTACAGGACTATTTACAAGAGGATTAACTCAAGT
AATGACAGCTGCTACCATTGGACCTTTAGCAGATGTACAAATTCTCGATAGTATAGAAGATGAAGAATTTAAAAGATATA
TGCATCACTATAACTTTCCTTCTTACAGTGTAGGGGAAACTAGACCTTTAAGAGGACCAGGTAGAAGAGAAATAGGTCAT
GGAGCTTTAGCAGAAAAAGCATTAGAACCATTAATACCATCTGAAGAAGATTTCCCATATACTATAAGACTTGTATCCGA
AGTTTTAAGTTCAAATGGATCTACATCACAGGCAAGTGTTTGTGGGAGTACACTAGCATTATTAGATGCAGGTGTACCTA
TAAAAAGACCAGCAGCAGGAATAGCTATGGGGCTTGTTACTAATGATGATTTAACAGAAGAAAAAATTTTAACTGATATA
CAAGGCATAGAAGACTTTTTTGGAGATATGGACTTTAAAGTTGCGGGTACAGAAAAAGGAATAACCGCTCTACAAGTAGA
TACAAAGATAAGTGGATTATCAGACAACTGTATAAAAGAAACCTTAATAAAAGCAAAAGATGCTAGAATGTATATATTAG
GTAAAATTAAAGAGTGTATACCAGAGCACAGAGCAGAACTTTCACCTTATGCACCAAGAGTCTATACTATGACTATAGCA
CCAGAAAAAATAAGAGATGTAATAGGTGCTGGTGGTAAAACAATAAATAAAATAATAGGTGAAACAGGAGTTCAAATAGA
CATAAAAGAAGATGGAAAAATATATGTTATGTCTTCTGATAGTGTAGGAGCTAATAGAGCTTTAAAGATGATAAATGATT
TAACTAAAGATGTGGAAGCAGGAGAAATTTATTTAGGTAAAGTTACTAGAACAACTAATTTTGGGGCTTTTGTTGAAATA
CTTCCAGGAAAAGAAGGATTAGTACACATTTCAAAATTAGATTTTACAAGAGTAAATAAGGTTGAAGATGTGGTTTCAGT
TGGAGATGAAATATTAGTAAAAGTAACAGATATAGATGATCAAGGAAGAATAAATCTTTCAAGAAGAGATGCAATAAAGA
AAGAAGAAGACGAAAAAACTAAAGAAAATAATCATTAA

Upstream 100 bases:

>100_bases
GGTAGAGCGGCTTTGACCGCTCTATTATTTTGAACAATGTATTTTATATAAAATTAATTTTAGTTTTAATCAAAATTATG
GTTTCAAAAGGAGGTAAACT

Downstream 100 bases:

>100_bases
AATAGAAGGGCTTTGTGCCTTTTTATTTTTTAAATGGAATATTATAAAATTTATGGAGGAATTTATGTATAAACTATATA
GCCTCAATAACGGATTAAGA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 705; Mature: 704

Protein sequence:

>705_residues
MSHVLETDIAGRTLKVEYGKVGMLSNCAILVSYGDTVVLINANASKEPRDGIDFFPLSVEYEERLYSVGKIPGGFIKREG
RPTENAILHARAIDRPLRPLFPKGYRNDVQIVCTVLSVESDNQPDILAMNGASLALCLSSIPFTTPVANVSVGLINGDFI
LNPTQSQREESILNLTVSATKERVMMIEAGAEEVDEDTMYNAIRFGFEQCQKIVEFQEEAMRRFGKEKVEPVLYLVDKDI
EKEVREFAFPMVKEAMYISDKDLRNEKIDEVKEKVKEEFEEKYPENDSDISEALYKMQKEVVRNMILREKRRPDERAFDE
IRKISCEVGLLPRTHGTGLFTRGLTQVMTAATIGPLADVQILDSIEDEEFKRYMHHYNFPSYSVGETRPLRGPGRREIGH
GALAEKALEPLIPSEEDFPYTIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLVTNDDLTEEKILTDI
QGIEDFFGDMDFKVAGTEKGITALQVDTKISGLSDNCIKETLIKAKDARMYILGKIKECIPEHRAELSPYAPRVYTMTIA
PEKIRDVIGAGGKTINKIIGETGVQIDIKEDGKIYVMSSDSVGANRALKMINDLTKDVEAGEIYLGKVTRTTNFGAFVEI
LPGKEGLVHISKLDFTRVNKVEDVVSVGDEILVKVTDIDDQGRINLSRRDAIKKEEDEKTKENNH

Sequences:

>Translated_705_residues
MSHVLETDIAGRTLKVEYGKVGMLSNCAILVSYGDTVVLINANASKEPRDGIDFFPLSVEYEERLYSVGKIPGGFIKREG
RPTENAILHARAIDRPLRPLFPKGYRNDVQIVCTVLSVESDNQPDILAMNGASLALCLSSIPFTTPVANVSVGLINGDFI
LNPTQSQREESILNLTVSATKERVMMIEAGAEEVDEDTMYNAIRFGFEQCQKIVEFQEEAMRRFGKEKVEPVLYLVDKDI
EKEVREFAFPMVKEAMYISDKDLRNEKIDEVKEKVKEEFEEKYPENDSDISEALYKMQKEVVRNMILREKRRPDERAFDE
IRKISCEVGLLPRTHGTGLFTRGLTQVMTAATIGPLADVQILDSIEDEEFKRYMHHYNFPSYSVGETRPLRGPGRREIGH
GALAEKALEPLIPSEEDFPYTIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLVTNDDLTEEKILTDI
QGIEDFFGDMDFKVAGTEKGITALQVDTKISGLSDNCIKETLIKAKDARMYILGKIKECIPEHRAELSPYAPRVYTMTIA
PEKIRDVIGAGGKTINKIIGETGVQIDIKEDGKIYVMSSDSVGANRALKMINDLTKDVEAGEIYLGKVTRTTNFGAFVEI
LPGKEGLVHISKLDFTRVNKVEDVVSVGDEILVKVTDIDDQGRINLSRRDAIKKEEDEKTKENNH
>Mature_704_residues
SHVLETDIAGRTLKVEYGKVGMLSNCAILVSYGDTVVLINANASKEPRDGIDFFPLSVEYEERLYSVGKIPGGFIKREGR
PTENAILHARAIDRPLRPLFPKGYRNDVQIVCTVLSVESDNQPDILAMNGASLALCLSSIPFTTPVANVSVGLINGDFIL
NPTQSQREESILNLTVSATKERVMMIEAGAEEVDEDTMYNAIRFGFEQCQKIVEFQEEAMRRFGKEKVEPVLYLVDKDIE
KEVREFAFPMVKEAMYISDKDLRNEKIDEVKEKVKEEFEEKYPENDSDISEALYKMQKEVVRNMILREKRRPDERAFDEI
RKISCEVGLLPRTHGTGLFTRGLTQVMTAATIGPLADVQILDSIEDEEFKRYMHHYNFPSYSVGETRPLRGPGRREIGHG
ALAEKALEPLIPSEEDFPYTIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLVTNDDLTEEKILTDIQ
GIEDFFGDMDFKVAGTEKGITALQVDTKISGLSDNCIKETLIKAKDARMYILGKIKECIPEHRAELSPYAPRVYTMTIAP
EKIRDVIGAGGKTINKIIGETGVQIDIKEDGKIYVMSSDSVGANRALKMINDLTKDVEAGEIYLGKVTRTTNFGAFVEIL
PGKEGLVHISKLDFTRVNKVEDVVSVGDEILVKVTDIDDQGRINLSRRDAIKKEEDEKTKENNH

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=710, Percent_Identity=37.3239436619718, Blast_Score=451, Evalue=1e-126,
Organism=Escherichia coli, GI145693187, Length=686, Percent_Identity=44.8979591836735, Blast_Score=606, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI115534063, Length=704, Percent_Identity=34.5170454545455, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI281362905, Length=703, Percent_Identity=38.4068278805121, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651641, Length=703, Percent_Identity=38.4068278805121, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651643, Length=703, Percent_Identity=38.4068278805121, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI161079377, Length=648, Percent_Identity=39.1975308641975, Blast_Score=421, Evalue=1e-118,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_CLOTE (Q895J3)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781910.1
- HSSP:   P05055
- ProteinModelPortal:   Q895J3
- SMR:   Q895J3
- GeneID:   1059391
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC01280
- NMPDR:   fig|212717.1.peg.1132
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q895J3
- ProtClustDB:   PRK11824
- BioCyc:   CTET212717:CTC_01280-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 78552; Mature: 78421

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHVLETDIAGRTLKVEYGKVGMLSNCAILVSYGDTVVLINANASKEPRDGIDFFPLSVE
CCCCCCCCCCCCEEEEECCCCCEECCCEEEEEECCEEEEEECCCCCCCCCCCCEEEEEEE
YEERLYSVGKIPGGFIKREGRPTENAILHARAIDRPLRPLFPKGYRNDVQIVCTVLSVES
HHHHHHHHCCCCCCHHCCCCCCCHHHEEEHHHHCCCCCCCCCCCCCCCCEEEEEEEEECC
DNQPDILAMNGASLALCLSSIPFTTPVANVSVGLINGDFILNPTQSQREESILNLTVSAT
CCCCCEEEECCCHHHHHHHCCCCCCCCCCCEEEEEECCEEECCCHHHHHHHHHEEEEECC
KERVMMIEAGAEEVDEDTMYNAIRFGFEQCQKIVEFQEEAMRRFGKEKVEPVLYLVDKDI
CCEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCEEEEECCHH
EKEVREFAFPMVKEAMYISDKDLRNEKIDEVKEKVKEEFEEKYPENDSDISEALYKMQKE
HHHHHHHHHHHHHHHHHCCCCHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VVRNMILREKRRPDERAFDEIRKISCEVGLLPRTHGTGLFTRGLTQVMTAATIGPLADVQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
ILDSIEDEEFKRYMHHYNFPSYSVGETRPLRGPGRREIGHGALAEKALEPLIPSEEDFPY
HHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCH
TIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLVTNDDLTEEKILTDI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHH
QGIEDFFGDMDFKVAGTEKGITALQVDTKISGLSDNCIKETLIKAKDARMYILGKIKECI
HHHHHHHCCCCEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHH
PEHRAELSPYAPRVYTMTIAPEKIRDVIGAGGKTINKIIGETGVQIDIKEDGKIYVMSSD
HHHHHCCCCCCCEEEEEEECHHHHHHHHCCCCHHHHHHHCCCCCEEEEECCCEEEEEECC
SVGANRALKMINDLTKDVEAGEIYLGKVTRTTNFGAFVEILPGKEGLVHISKLDFTRVNK
CCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCEEEEEECCCCCEEEEEECCCHHHHH
VEDVVSVGDEILVKVTDIDDQGRINLSRRDAIKKEEDEKTKENNH
HHHHHHCCCEEEEEEECCCCCCCEECHHHHHHCCHHHHHHHCCCC
>Mature Secondary Structure 
SHVLETDIAGRTLKVEYGKVGMLSNCAILVSYGDTVVLINANASKEPRDGIDFFPLSVE
CCCCCCCCCCCEEEEECCCCCEECCCEEEEEECCEEEEEECCCCCCCCCCCCEEEEEEE
YEERLYSVGKIPGGFIKREGRPTENAILHARAIDRPLRPLFPKGYRNDVQIVCTVLSVES
HHHHHHHHCCCCCCHHCCCCCCCHHHEEEHHHHCCCCCCCCCCCCCCCCEEEEEEEEECC
DNQPDILAMNGASLALCLSSIPFTTPVANVSVGLINGDFILNPTQSQREESILNLTVSAT
CCCCCEEEECCCHHHHHHHCCCCCCCCCCCEEEEEECCEEECCCHHHHHHHHHEEEEECC
KERVMMIEAGAEEVDEDTMYNAIRFGFEQCQKIVEFQEEAMRRFGKEKVEPVLYLVDKDI
CCEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCEEEEECCHH
EKEVREFAFPMVKEAMYISDKDLRNEKIDEVKEKVKEEFEEKYPENDSDISEALYKMQKE
HHHHHHHHHHHHHHHHHCCCCHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VVRNMILREKRRPDERAFDEIRKISCEVGLLPRTHGTGLFTRGLTQVMTAATIGPLADVQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
ILDSIEDEEFKRYMHHYNFPSYSVGETRPLRGPGRREIGHGALAEKALEPLIPSEEDFPY
HHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCH
TIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLVTNDDLTEEKILTDI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHH
QGIEDFFGDMDFKVAGTEKGITALQVDTKISGLSDNCIKETLIKAKDARMYILGKIKECI
HHHHHHHCCCCEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHH
PEHRAELSPYAPRVYTMTIAPEKIRDVIGAGGKTINKIIGETGVQIDIKEDGKIYVMSSD
HHHHHCCCCCCCEEEEEEECHHHHHHHHCCCCHHHHHHHCCCCCEEEEECCCEEEEEECC
SVGANRALKMINDLTKDVEAGEIYLGKVTRTTNFGAFVEILPGKEGLVHISKLDFTRVNK
CCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCEEEEEECCCCCEEEEEECCCHHHHH
VEDVVSVGDEILVKVTDIDDQGRINLSRRDAIKKEEDEKTKENNH
HHHHHHCCCEEEEEEECCCCCCCEECHHHHHHCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129