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The map label for this gene is ispG

Identifier: 28210956

GI number: 28210956

Start: 1359571

End: 1360620

Strand: Direct

Name: ispG

Synonym: CTC01270

Alternate gene names: 28210956

Gene position: 1359571-1360620 (Clockwise)

Preceding gene: 28210955

Following gene: 28210957

Centisome position: 48.57

GC content: 31.71

Gene sequence:

>1050_bases
TTGGATAGAAAAGAAACTATAAAGATAAAGGTAGGAGACAAATTTATAGGGGGGGATTCTCCTATAACAGTTCAATCCAT
GACCAATACAGATACAAGAGATGTAGAAAAAACTGTTGAGCAAATAAAAAAATTAGAAGAAGTTGATTGCGATATAATAA
GATGTGCAGTTCCAGATGATGAAGCTTGCCAAGCTTTAGGCAAAATAACAAAAAAAATTGCAATACCATTAGTAGCAGAT
ATACATTTTGATTATAGATTAGCTATAAAAGCTATAGATAATGGGATATCTGCCCTAAGAATAAACCCTGGCAATATAGG
AAATGAAGAAAAGGTTAATTTAGTTTCAAAAAAAGCTAAAGAAAAAGGAATACCTATAAGAATAGGAGTAAACTCTGGTT
CTTTAGAAAAGGATATATTAAATAAATATGGTGGAGTTTGTTCAGATGCTTTAGTGGAAAGTGCATTAAGACATGTTGAA
ATTTTAAATAAAGTGGATTTTCATGATATAGTTATATCCATTAAGTCTTCAAATGTAAACCAAATGATAGAAAGCTATAA
AAAAATATCTTCAAAAGTAAATTATCCTCTACACTTAGGTGTAACGGAAGCTGGAACACCTTGGAGGGGTATAATAAAAT
CTAGTGTAGGTATAGGAACTTTACTATCTCAAGGTATAGGGGATACTATAAGGGTATCTTTGACAGGAGACCCTATAGAA
GAAATTAAAGCAGGAAAAGAGATCTTAAGAGCTTTGAATTTATATAGTAAGGGCGTAGAATTTATTTCTTGCCCTACTTG
TGGAAGAACAGAGATAAATTTAATAAAAATAGCCAAAGAAGTTGAAAAAAGACTTGATGGCTTTGATAAAAATATTAAAG
TAGCTATTATGGGTTGCGTGGTAAATGGACCTGGAGAAGCTAGGGATGCAGACATAGGCATTGCAGGTGGAAAGGGCTAT
GGTTTAATATTTAAAAAGGGAGAAGTAGTAAGAAAGCTTAAAGAGGAAGATTTAGTAGAAGGGCTTATAGAAGAAATAAA
AAATATATAG

Upstream 100 bases:

>100_bases
TGTAATAAATTATATAGGATTTGCCATACTAATGACATTGATGGTACTAGTAACGGTAAAAGATATACTATATCCTATAA
ACTTTTAGGAGTGAATTTTA

Downstream 100 bases:

>100_bases
GATGTGAAGGGTATGAGTTGGTATGGAATTAGCTAAAATATTAGAAGACAATACTTTTTCACAAGATAGTTTAGAGGGCA
TAAGAGTCCATAGAATACAG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MDRKETIKIKVGDKFIGGDSPITVQSMTNTDTRDVEKTVEQIKKLEEVDCDIIRCAVPDDEACQALGKITKKIAIPLVAD
IHFDYRLAIKAIDNGISALRINPGNIGNEEKVNLVSKKAKEKGIPIRIGVNSGSLEKDILNKYGGVCSDALVESALRHVE
ILNKVDFHDIVISIKSSNVNQMIESYKKISSKVNYPLHLGVTEAGTPWRGIIKSSVGIGTLLSQGIGDTIRVSLTGDPIE
EIKAGKEILRALNLYSKGVEFISCPTCGRTEINLIKIAKEVEKRLDGFDKNIKVAIMGCVVNGPGEARDADIGIAGGKGY
GLIFKKGEVVRKLKEEDLVEGLIEEIKNI

Sequences:

>Translated_349_residues
MDRKETIKIKVGDKFIGGDSPITVQSMTNTDTRDVEKTVEQIKKLEEVDCDIIRCAVPDDEACQALGKITKKIAIPLVAD
IHFDYRLAIKAIDNGISALRINPGNIGNEEKVNLVSKKAKEKGIPIRIGVNSGSLEKDILNKYGGVCSDALVESALRHVE
ILNKVDFHDIVISIKSSNVNQMIESYKKISSKVNYPLHLGVTEAGTPWRGIIKSSVGIGTLLSQGIGDTIRVSLTGDPIE
EIKAGKEILRALNLYSKGVEFISCPTCGRTEINLIKIAKEVEKRLDGFDKNIKVAIMGCVVNGPGEARDADIGIAGGKGY
GLIFKKGEVVRKLKEEDLVEGLIEEIKNI
>Mature_349_residues
MDRKETIKIKVGDKFIGGDSPITVQSMTNTDTRDVEKTVEQIKKLEEVDCDIIRCAVPDDEACQALGKITKKIAIPLVAD
IHFDYRLAIKAIDNGISALRINPGNIGNEEKVNLVSKKAKEKGIPIRIGVNSGSLEKDILNKYGGVCSDALVESALRHVE
ILNKVDFHDIVISIKSSNVNQMIESYKKISSKVNYPLHLGVTEAGTPWRGIIKSSVGIGTLLSQGIGDTIRVSLTGDPIE
EIKAGKEILRALNLYSKGVEFISCPTCGRTEINLIKIAKEVEKRLDGFDKNIKVAIMGCVVNGPGEARDADIGIAGGKGY
GLIFKKGEVVRKLKEEDLVEGLIEEIKNI

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=347, Percent_Identity=48.4149855907781, Blast_Score=349, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_CLOTE (Q895K3)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781900.1
- ProteinModelPortal:   Q895K3
- GeneID:   1059132
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC01270
- NMPDR:   fig|212717.1.peg.1122
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- PhylomeDB:   Q895K3
- ProtClustDB:   PRK00366
- BioCyc:   CTET212717:CTC_01270-MONOMER
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 37991; Mature: 37991

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRKETIKIKVGDKFIGGDSPITVQSMTNTDTRDVEKTVEQIKKLEEVDCDIIRCAVPDD
CCCCEEEEEEECCEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCH
EACQALGKITKKIAIPLVADIHFDYRLAIKAIDNGISALRINPGNIGNEEKVNLVSKKAK
HHHHHHHHHHHHHHHHEEEEECCCCEEEEEEHHCCCEEEEECCCCCCCHHHHHHHHHHHH
EKGIPIRIGVNSGSLEKDILNKYGGVCSDALVESALRHVEILNKVDFHDIVISIKSSNVN
HCCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCHH
QMIESYKKISSKVNYPLHLGVTEAGTPWRGIIKSSVGIGTLLSQGIGDTIRVSLTGDPIE
HHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHCCCHHHHHHCCCCCEEEEEECCCCHH
EIKAGKEILRALNLYSKGVEFISCPTCGRTEINLIKIAKEVEKRLDGFDKNIKVAIMGCV
HHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
VNGPGEARDADIGIAGGKGYGLIFKKGEVVRKLKEEDLVEGLIEEIKNI
ECCCCCCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDRKETIKIKVGDKFIGGDSPITVQSMTNTDTRDVEKTVEQIKKLEEVDCDIIRCAVPDD
CCCCEEEEEEECCEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCH
EACQALGKITKKIAIPLVADIHFDYRLAIKAIDNGISALRINPGNIGNEEKVNLVSKKAK
HHHHHHHHHHHHHHHHEEEEECCCCEEEEEEHHCCCEEEEECCCCCCCHHHHHHHHHHHH
EKGIPIRIGVNSGSLEKDILNKYGGVCSDALVESALRHVEILNKVDFHDIVISIKSSNVN
HCCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCHH
QMIESYKKISSKVNYPLHLGVTEAGTPWRGIIKSSVGIGTLLSQGIGDTIRVSLTGDPIE
HHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHCCCHHHHHHCCCCCEEEEEECCCCHH
EIKAGKEILRALNLYSKGVEFISCPTCGRTEINLIKIAKEVEKRLDGFDKNIKVAIMGCV
HHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
VNGPGEARDADIGIAGGKGYGLIFKKGEVVRKLKEEDLVEGLIEEIKNI
ECCCCCCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129